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Denis Wirtz 265 Garland Hall 3400 N. Charles Street Baltimore, MD 21218 Office: (410) 516-8094 wirtz@jhu.edu Languages Spoken: Fluent in English and French Citizenship: United States of America and Belgium

I. EDUCATION

1993-1994 Postdoctoral Fellow, ESPCI, Paris, France 1989-1993 Ph.D., Chemical Engineering, Stanford University, CA

Thesis Advisor: Professor Gerald. G. Fuller 1988-1989 M.Sc., Chemical Engineering, Stanford University, CA 1983-1988 Eng. Deg., Physics Engineering, Ecole Polytechnique,

Université libre de Bruxelles, Belgium Research Advisor: Professor Jean-Claude Legros

II. PROFESSIONAL EXPERIENCE

February 2014-present Vice Provost for Research, Johns Hopkins University December 2012-present Professor (Joint Appointment), Department of Pathology,

Johns Hopkins School of Medicine September 2010-present Founder and Director, National Cancer Institute R25

Predoctoral Training Program in Nanotechnology for Cancer Medicine

December 2009-present Theophilus Halley Smoot Professor of Engineering Science, Whiting School of Engineering, The Johns Hopkins University

October 2009-present Founder and Director, Physical Sciences in Oncology Center, National Cancer Institute U54 center grant, The Johns Hopkins University

July 2008-present Professor (Joint Appointment), Department of Oncology, Johns Hopkins School of Medicine

July 2008-present Member, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine

July 2008-present Founder and Director, National Cancer Institute T32 postdoctoral training program in nanotechnology for cancer medicine, The Johns Hopkins University

July 2008-present Director, NSF REU program in nanotechnology for biology, The Johns Hopkins University

May 2008-present Visiting Professor, National University of Singapore, Institute of Mechanobiology, NUS, Singapore

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May 2006-present Co-Founder and co-Director, Johns Hopkins Institute for Nanobiotechnology (INBT)

December 2005-2010 Founder and Director, HHMI graduate training program in nanobiotechnology, The Johns Hopkins University

April 2004-present Professor (Joint Appointment), Department of Materials Science and Engineering, The Johns Hopkins University

April 2004-2009 Professor, Department of Chemical and Biomolecular Engineering, The Johns Hopkins University

January 2001-April 2004 Associate Professor, Department of Chemical and Biomolecular Engineering, The Johns Hopkins University

January 2001-2003 Associate Professor (Joint Appointment), Department of Materials Science and Engineering, The Johns Hopkins University

November 1998-J. 2001 Assistant Professor (Joint Appointment), Department of Materials Science and Engineering, The Johns Hopkins University

July 1994- January 2001 Assistant Professor, Department of Chemical Engineering, The Johns Hopkins University

April 1993-May 1994 European Union Post-Doctoral Fellow, Ecole Supérieure de Physique et de Chimie Industrielles (ESPCI), Paris, France

III. AWARDS AND HONORS

2017 Distinguished lecture, Perlmann Comprehensive Cancer Center, University of Pennsylvania

2017 Visiting Scholar, Israel 2015 Plenary talk, Materials Research Society (MRS)

Meeting, Boston, MA 2015 Plenary talk, 2015 Cell Mechanics, Morphogenesis and

Pattern Formation Workshop, Isaac Newton Institute, University of Cambridge

2015 Keynote talk, The American Society of Mechanical Engineers (ASME), Global Congress on NanoEngineering for Medicine and Biology, Minneapolis, MN

2015 Plenary talk, American Society for Cell Biology (ASCB), San Diego, CA

2014 Plenary talk, Annual Longrifles Meeting, Johns Hopkins School of Medicine, Departments of Oncology and Pathology

2014 Plenary talk, International Union of Theoretical and

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Applied Mechanics (IUTAM) Symposium on Mechanics of Soft Active Materials

2013 Plenary talk, The Center for Neuroscience and Cell Biology (CNC), annual conference, Coimbra, Portugal

2013 Plenary talk, The American Society for Mechanical Engineers, Global Congress on NanoEngineering for Medicine and Biology, Boston

2012 Distinguished Lecturer, Cancer Nanotechnology Training Center (CNTC), University of Kentucky

2011 Section 15c/d Prize of the American Institute of Chemical Engineering (AIChE)

2011-present Elected member of the Belgian American Educational Foundation (BAEF)

2010-present Elected Fellow, American Physical Society (APS) 2009-present Theophilus H. Smoot Professorship in Engineering

Science, Whiting School of Engineering, Johns Hopkins University; Selected by President Ronald J. Daniels and the Board of Trustees

2009-present Elected Fellow, American Association for the Advancement of Science (AAAS)

2009 Schwarz Lectureship, Johns Hopkins University 2008-present “Nikon Partner in Science”, Nikon Inc. 2008 Member, National Cancer Institute Advisory Panel on

Physics of Cancer 2008 Member, National Science Foundation Advisory Panel on

Mechanobiology 2006-present Member, Tumor Microenvironment Study Section, NIH 2005-present Elected Fellow, American Institute for Medical and

Biological Engineering (AIMBE) 2004-present Member, American Society for Microbiology 2004 Outstanding Invention of 2004, Office of Technology

Commercialization, University of Maryland, “Cell sensor based pathogen detection”

2002-2006 NSE/NIRT Award, National Science Foundation 2000-present Member, American Society for Cell Biology 2000-present Member, American Physical Society 1998-present Member, Biophysical Society 1996 CAREER Award, National Science Foundation 1995-present Member, American Institute of Chemical Engineers

(AIChE) 1995-present Member, Society of Rheology 1993-1994 Fellow, Human Capital and Mobility program, European

Union 1989 Claes Prize, for best Engineering/Science Dissertation,

Royal Academy of Sciences, Letters, and Arts, Belgium

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1988-1989 Hoover Fellow, Belgian American Educational Foundation

IV. EDITORIAL BOARDS, EDITING

2018-present Editorial Board Member, Science Advances, a AAAS journal

2016-present Editorial Board Member, Cancer Convergence, a Springer- Nature journal

2013-present Editorial Board Member, Technology 2012-present Editorial Board Member, Journal of the Royal Society

Interface Focus 2012-2015 Expert Advisor to the study panel of the international

Assessment of Physical sciences and Engineering advances in Life sciences and Oncology (APHELION), commissioned by the NCI and NSF

2012 Editor, Comprehensive Biophysics, Volume 7: Cell Biophysics, Elsevier B.V.

2011-present Editorial Board Member, Scientific Reports, a Nature journal

2011 Guest Editor of a Special Issue of Physical Biology on Physical Oncology

2010 Ad Hoc Editor, PNAS 2009-2012 Editorial Board Member, Physical Biology 2008-2015 Editor-in-Chief, Cell Health and the Cytoskeleton 2007-2013 Editorial Board Member, Biophysical Journal 2006-2014 Editorial Board Member, Cell Adhesion & Migration 2006-2009 Editorial Board Member, Nanomedicine: Nanotechnology,

Biology and Medicine V. MAJOR CONTRIBUTIONS TO SCIENCE (last 5 years)

• 2D vs. 3D Cell Migration We have identified fundamental differences between conventional 2D migration and more physiologically relevant 3D migration. In particular, we have found that the nucleus, focal adhesion molecules, the lamina, LINC complexes, and MMPs play a central role in 3D migration (migration in 3D collagen-rich matrices) that is not predicted or extrapolated from the better known 2D case.

• Cell and Nuclear Mechanics We have developed novel tools and concepts to study the role of nucleus and nuclear connections to the cytoskeleton and key cell functions, including 2D and 3D cell migration and adhesion, mechanosensation, and mechanotransduction. In particular, we have identified LINC complexes and the so-called perinuclear actin cap as key mediators of physical signaling between the cytoplasm and the nuclear

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interior. More recently, we gave discovered that the nuclear lamina of adherent cells in polarized through the actin cap, which in turn polarizes hyper-acetylated forms of histones in the nucleus.

VI. MAJOR CONTRIBUTIONS TO ADMINISTRATION and CURRICULUM DEVELOPMENT 2017-present Johns Hopkins Office for Undergraduate Research (HOUR)

Founded, staffed, and oversee the Johns Hopkins Office for Undergraduate Research (HOUR), which launched the STAR program for undergraduate research at Hopkins during the summer with programming on the scientific method, research integrity and ethics; the CIRCUIT program for summer undergraduate research at the Applied Physics Lab (APL); increased greatly the number and size of Provost Undergraduate Research Awards (PURA) for Johns Hopkins undergraduate students during the year, founded the annual day of research, arts, medicine and science DREAMS, a one-day celebration of undergraduate research and performance involving >300 undergraduate students; greatly increased access to undergraduate research at Hopkins to URM and first-generation students through outreach. New HOUR website which list opportunities and awards; new HOUR Office for advising of students

2017-present Research Development Team (RDT) Founded, staffed, and oversee the Research Development Team (RDT) to administratively support teams of faculty to apply for large center/projects grants

2017-2018 JHU Research Administration (JHURA) Major re-organization, re-staffing, signature authorities of Johns Hopkins Research Administration (JHURA), which has 28 professional staff overseeing the submission of ~2000 grants and contracts a year by 9 schools at Hopkins, APL, and JHPiego

2017 Website of the VP Research office Created the website for the VP Research office, which maintains extensive lists of external and internal funding opportunities, and describes JHURA, HOUR, and RDT, a well as research compliance and integrity.

2015-present The Catalyst Award program Founded and oversee the Catalyst Award program, which supports the research of 35 early-career faculty at Hopkins for a total of $3M a year. Promotes new high-risk research and retention of star junior faculty. Programming on mentorship, lab staffing and direction, etc.

2015-present The Discovery Award program Founded and oversee the Discovery Award program, which every funds the multidisciplinary research of 23 teams of faculty for a total of $2M a year. Coordinates the administrative support of Discovery teams by the RDT.

2015-present Databases of funding opportunities and weekly emails Created large, searchable databases of funding opportunities for undergraduate students, PhD students, postdoctoral students, early-career faculty, large center/project grants, and internal funding opportunities. Weekly emails to 15,000 Johns Hopkins

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Deans, chairs, faculty, staff, and students/fellows; also listed on Research website. Managed access to previously successful grants

2014-present Bloomberg Distinguished Professorship program Oversee the $250M Bloomberg Distinguished Professorship program, which recruits and supports 50 new cross-divisionally appointed professors at Hopkins.

2014-present Signature Initiatives Oversee the administration of the $20M Signature Initiatives, which are seven large multi-divisional research programs and institutes, including 21 Century Cities, Space, the Alliance for a Healthier World, and Brain Sciences

VII. PUBLICATIONS Google Scholar, citations >17,274; h-index 74; Scopus: citations >12,454, h-index 64 (209) P.-H. Wu, D.M. Gilkes, and D. Wirtz, “The biophysics of 3D cell migration”,

Annual Review in Biophysics (2018), to appear (208) P.-H. Wu, D. Raz-Ben Aroush, A. Asnacios, W.-C. Chen, M.E. Dokukin, B.L.

Doss, P. Durand, A. Ekpenyong, J. Guck, N. Guz, P.A. Janmey, N.M. Moore, A. Ott, Y.-C. Poh, R. Ros, M. Sander, I. Sokolov, J.R. Staunton, N. Wang, G. Whyte, and D. Wirtz, “Comparative study of cell mechanics methods”, Nature Methods (2018), to appear

(207) Y‐A. Lin, M. Kang, W.‐C. Chen, Y.‐C. Ou, A.G. Cheetham, P.‐ H. Wu, D. Wirtz, S.M. Loverde, and H. Cui , “Isomeric Control of the mechanical properties of supramolecular filament hydrogels”, Biomaterials Science, to appear (2018)

(206) B. Koons, P. Sharma, Z. Ye, A. Mukherjee, M.H. Lee, D. Wirtz, B. Behkam, A. Singh Nain, “Cancer protrusions on a tightrope: Nanofiber curvature contrast quantitates single protrusion dynamics”, ACS Nano, to appear (2017)

(205) H. Jayatilaka, A. Giri, M. Karl, I. Aifuwa, N.J. Trenton, J.M. Phillip, S. Khatau, and D. Wirtz, “EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient three-dimensional cell migration”, FASEB Journal, to appear (2017)

(204) J.-K. Kim, A. Louhghalam, G. Lee, B.W. Schafer, D. Wirtz, and D.H. Kim. “Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology”, Nature Communications 8:2123-2135 (2017)

(203) J.S. Sarnecki, L.D. Wood, C.L. Wolfgang, R.H. Hruban, A. Maitra, D. Wirtz, and P.-H. Wu

, “Computational discovery of tissue morphology biomarkers in very long-term survivors with pancreatic ductal adenocarcinoma”, Biorxiv 2017

(202) Y. Li, L. He, N.A.P. Gonzalez, J. Graham, C. Wolgemuth, D. Wirtz, and S.X. Sun, “Going with the flow: Water flux and cell shape during cytokinesis”, Biophysical Journal 113, 2487–2495 (2017)

(201) L. He, A. Sneider, W. Chen, M. Karl, V. Prasath, P.-H. Wu, G. Mattson, and D. Wirtz, “Mammalian cell Division in 3D matrices via quantitative confocal reflection microscopy”, Journal of Visual Experiments (2017)

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(200) J.M. Phillip, P.-H. Wu, D.M. Gilkes, W. Williams, S. McGovern, J. Daya, J.S.H. Lee, J. Chen, I. Aifuwa, R. Fan, J. Walston, and D. Wirtz, “Determination of cellular age in human health and disease”, Nature Biomedical Engineering 1: 93-105 (2017)

(199) T. Perestrelo, W. Chen, M. Correia, C. Le, S. Pereira, A.S. Rodrigues, M.I. Sousa J. Ramalho-Santos, and D. Wirtz, “Pluri-IQ: Quantification of Embryonic Stem Cell Pluripotency Through an Image Based Analysis Software”, Stem Cell Reports 9: 697-709 (2017).

(198) Y. Wang, J. Gong, D. Wirtz, and B.W. Schafer, “Affine and non-affine deformations quantified in cytoskeletal networks through three-dimensional form-finding model”, Journal of the Mechanical Behavior of Biomedical Materials, 72: 52-65 (2017)

(197) D.M. Gilkes and D. Wirtz, “Tumour mechanopathology: Cutting the stress out”, Nature Biomedical Engineering 1, 12-14 (2017)

(196) H. Ahmadzadeh, M.R. Webster, R. Behera, A.M. Jimenez Valencia, D. Wirtz, A.T. Weeraratna, and V.B. Shenoy “Modeling the two-way feedback between contractility and matrix realignment reveals a nonlinear mode of cancer cell invasion”, Proceedings of the National Academy of Sciences E1617-E1626 (2017)

(195) H. Jayatilaka, P.Tyle, J.J. Chen, M. Kwak, J.A. Ju, H.J. Kim, J.S.H. Lee, P.-H. Wu, D.M. Gilkes, R. Fan, and D. Wirtz , “Novel synergistic IL6/8 paracrine signaling pathway infers new strategy to inhibit tumor cell migration”, Nature Communications 8: 15584-15596 (2017)

(194) J.A Ju, I. Godet, I.C. Ye, J. Byun, H. Jayatilaka, S.J. Lee, L. Xiang, D. Samanta, M.H. Lee, P-H. Wu, D. Wirtz, G.L. Semenza, and D.M. Gilkes, “Hypoxia selectively enhances integrin receptor expression to promote metastasis”, Molecular Cancer Research 0338.2016 (2017)

(193) A. Agarwal, P.-H. Wu, E.G. Hughes, M.A. Tischfield, M. Fukaya, J. Nathans, D. Wirtz, and D.E. Bergles, “Mitochondria generate microdomain Ca2+ transients in astrocytes”, Neuron 93: 587-605 (2017)

(192) M.Y. Shah, E. Martinez-Garcia, J.M. Phillip, A.B. Chambliss, R. Popovic, T. Esponda, E.C. Small, C. Will, M.P. Phillip, P. Neri, N.J. Bahlis, D. Wirtz, J.D. Licht, “MMSET/WHSC1 enhances DNA damage repair leading to an increase in resistance to chemotherapeutic agents”, Oncogene 35: 5905-5915 (2016)

(191) W.-C. Wang, J.R. Yang, C.L. Yankaskas, B.S. Wong, P.-H. Wu, C. Pardo-Pastor, S.A. Sierra, M.-J. Chiang, Z. Gu, D. Wirtz, M.A. Valverde, J.T. Yang, J. Zhang, and K. Konstantopoulos, “Confinement Sensing and Signal Optimization via Piezo1/PKA and Myosin II Pathways”, Cell Reports 15:1430-1441 (2016)

(190) V.C. Hecht, L.B. Sullivan, R.J. Kimmerling, D.-H. Kim, A.M. Hosios, M.A. Stockslager, M.M. Stevens, J.H. Kang, D. Wirtz, M.G. Vander Heiden, and S.R. Manalis, “Biophysical changes reduce energetic demand in growth factor–deprived lymphocytes”, Journal of Cell Biology 212: 439-447 (2016)

(189) C.D. Paul, W.-C. Hung, D. Wirtz, and K. Konstantopoulos, “Engineered models of confined migration”, Annual Review of Biomedical Engineering 18: 159-180 (2016)

(188) Y. Gu, S.-L. Chiu, B. Liu, P.-H. Wu, M. Delannoy, D.-T. Lin, D. Wirtz, and R.L.

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Huganir, “Differential vesicular sorting of AMPA and GABA receptors”, Proceedings of the National Academy of Sciences 113: E922-E931 (2016)

(187) J.S. Sarnecki, K.H. Burns, L. D. Wood, K.M. Waters, R.H. Hruban, D. Wirtz, and P. Wu, “A robust non-linear tissue-component discrimination method for computational pathology”, Laboratory Investigation 96: 450-458 (2016)

(186) L. He, W. Chen, P.-H. Wu, A. Jimenez, B.S. Wong, A. San, K. Konstantopoulos, D. Wirtz, “Local 3D matrix confinement determines division axis through cell shape”, Oncotarget 7: 6994–7011 (2016)

(185) J.M. Phillip, I. Aifuwa, and D. Wirtz, “The mechanobiology of aging”, Annual Review of Biomedical Engineering 17: 113-141 (2015)

(184) A.M. Jimenez, P.-H. Wu, O. Yogurtcu, P. Rao, J. DiGiacomo, I. Godet, L. He, M.-H. Lee, D. Gilkes, S.X. Sun, and D. Wirtz, “Collective cancer cell invasion induced by coordinated contractile stress generated by multicellular spheroids”, Oncotarget (2015)

(183) L. He, W. Chen, P.-H. Wu, A. Jimenez, B.S. Wong, A. San, K. Konstantopoulos, D. Wirtz, “Local 3D matrix confinement determines division axis through cell shape”, Oncotarget (2015).

(182) D.J. Shea, D. Wirtz, K.J. Stebe, and K. Konstantopoulos “Distinct kinetic and mechanical properties govern mucin 16- and podocalyxin-mediated tumor cell adhesion to E- and L-selectin in shear flow”, Oncotarget (2015)

(181) S.I. Fraley, P.-H. Wu, L. He, Y. Feng, R. Krisnamurthy, G.D. Longmore, and D. Wirtz, “Three-dimensional matrix fiber alignment modulates cell migration and MT1-MMP utility by spatially and temporally directing protrusions”, Scientific Reports 5, 14580-14593 (2015)

(180) I. Aifuwa, A. Giri, N. Longe, S.H. Lee, S. An, and D. Wirtz, “Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility”, Oncotarget 6, 30515-30531 (2015).

(179) D.-H. Kim, B. Li, F. Si, J.M. Phillips, D. Wirtz*, and S.X. Sun*, “Volume regulation and shape bifurcation in the cell nucleus”, Journal of Cell Science 128, 3375-3385 (2015) (*=corresponding authors)

(178) M.-H. Lee, P.-H. Wu, D. Gilkes, I. Aifuwa, and D. Wirtz, “Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions”, Oncotarget 6, 32634-32645 (2015)

(177) B. Palmieri, Y. Bresler, D. Wirtz, and M. Grant, “M model for cell migration in monolayers: elastic mismatch between cells enhanced motility”, Scientific Reports 5: 11745-11757 (2015)

(176) Y. Yu, S. Gaillard, J.M. Phillip, T.-C. Huang, S.M Pinto, N.G. Tessarollo, Z. Zhang, A. Pandey, D. Wirtz, A. Ayhan, B. Davidson, T.-L. Wang, and I.-M. Shih, “Inhibition of spleen tyrosine kinase potentiates paclitaxel-induced cytotoxicity in ovarian cancer cells by stabilizing microtubules”, Cancer Cell 28: 82-96 (2015)

(175) A.-R. Yoon, I. Stasinopoulos, J.H. Kim, H.M. Yong, O. Kilic, D. Wirtz, Z. Bhujwalla, and S.S. An. “COX-2 dependent regulation of mechanotransduction in human breast cancer cells” Cancer Biology and Therapy 16: 430-437 (2015).

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(174) P.-H. Wu, A. Giri, and D. Wirtz, “Statistical analysis of cell migration in 3D using the anisotropic persistent random walk model”, Nature Protocols 10: 517–527 (2015).

(173) D.H. Kim and D. Wirtz, “Cytoskeletal tension induces the 3D polarized architecture of the nucleus”, Biomaterials 48: 161–172 (2015).

(172) L. He and D. Wirtz, “Switching from protease-independent to protease-dependent cancer cell invasion”, Biophysical Journal 107: 2484-2485 (2014)

(171) M. Castillo, R. Ebensperger, D. Wirtz, M. Walczak, D.E. Hurtado, and A. Celedon, “Local mechanical response of cells to the controlled rotation of magnetic rods”, Journal of Biomedical Materials Research Part B: Applied Biomaterials 102B: 1779–1785 (2014).

(170) D. Gilkes, G. Semenza, and D. Wirtz, “Hypoxia and the extracellular matrix: drivers of tumor metastasis”, Nature Reviews Cancer 14: 430-439 (2014)

(169) D.-H. Kim, S. Cho, and D. Wirtz, “Tight coupling between nucleus and cell migration through the perinuclear actin cap”, Journal of Cell Science127: 2528-2541 (2014).

(168) K.M. Stroka, H. Jiang, Z. Tong, D. Wirtz, S.X. Sun, and K. Konstantopoulos, “Water permeation drives tumor cell migration in confined microenvironments”, Cell 157: 611-623 (2014)

(167) P.-H. Wu, A. Giri, S.X. Sun, and D. Wirtz, “Three-dimensional cell migration does not follow a random walk”, Proceedings of the National Academy of Sciences 111: 3949-3954 (2014)

(166) D.M. Gilkes, L. Xiang, S.J. Lee, M.E. Hubbi, P. Chaturvedi, D. Wirtz, and G.L. Semenza, “Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 Eexpression and signaling in breast cancer cells”, Proceedings of the National Academy of Sciences 111: E384-E393 (2014)

(165) M.A Yong, Y. Zeng, D. Vindvich, J.M. Phillip, P.H. Wu, D. Wirtz, and R.H. Getzenberg “Morphological effects on expression of Growth Differentiation Factor 15 (GDF15), a marker of metastasis”, American Journal of Pathology 229: 362-373 (2014)

(164) D. Razafsky, D. Wirtz*, and D. Hodzic*, “Nuclear envelope in nuclear positioning and cell migration”, Advances in Experimental Medicine and Biology 773: 471-490 (2014) (*=corresponding authors)

(163) T.M. Dobrowsky, S.A. Rabi, R. Nedellec, B.R. Daniels, J.I. Mullin, D.E. Mosier, R.F. Siliciano, and D. Wirtz, “Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins”, Scientific Reports (Nature) 3: 3014 -3023 (2013)

(162) J. Gong, D. Zhang, Y. Tseng, B. Li, D. Wirtz, and B.W. Schafer. "Form-finding model shows how cytoskeleton network stiffness is realized”, PLOS One 8: e77417 (2013)

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(161) S. Bajpai, Y. Feng, D. Wirtz*, and G.D. Longmore*, “β-catenin serves as a clutch between low and high intercellular E-cadherin bond strengths ”, Biophysical Journal 105: 2289–2300 (2013) (*=corresponding authors)

(160) Q. Guo, J.M. Phillip, S. Majumdar, P.-H. Wu, J. Chen, S. Calderón-Colón, O. Schein, B.J. Smith, M.M. Trexler, D. Wirtz, and J.H. Elisseeff, “Collagen nanoarchitecture modulates keratocyte phenotype in vitrigel membranes for corneal reconstruction”, Biomaterials 34: 9365–9372 (2013)

(159) A. Giri, S. Bajpai, N. Trenton, H. Jayatilaka, G.D. Longmore, and D. Wirtz, “Multi-generation dendritic protrusions mediate three-dimensional cell migration”, FASEB Journal 27: 4089-99 (2013)

(158) Y. Lu, J.J. Chen, L. Mu, Q. Xue, Y. Wu, P.-H. Wu, J. Li, A. O. Vortmeyer, K. Miller-Jensen, D. Wirtz, and R. Fan , “High-Throughput secretomic analysis of single Cells to assess functional cellular heterogeneity”, Analytical Chemistry 85, 2548-2556 (2013)

(157) E.B. Stukalin, I. Aifuwa, J.S. Kim, D. Wirtz, and S.X. Sun, “Age-dependent stochastic models for understanding population fluctuations in continuously cultured cells”, Journal Royal Society Interface 10: 2013032z (2013)

(156) D.H. Kim, A.B. Chambliss, and D. Wirtz, “The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction”, Soft Matter 9: 5516-5523 (2013)

(155) D.H. Kim and D. Wirtz. “Predicting how cells spread and migrate: Focal adhesion size does matter”, Cell Adhesion and Migration 7:1-4 (2013)

(154) D.M Gilkes, P. Chaturvedi, S. Bajpai, C.C. Wong, H. Wei, S. Pitcairn, M.E. Hubbi, D. Wirtz, and S.L. Semenza, “Collagen prolyl hydroxylases are essential for breast cancer metastasis”, Cancer Research 73: 3285-3296 (2013)

(153) The PS-OC Cell-Line Project Team and the PS-OC Network, “A physical sciences network characterization of non-tumorigenic and metastatic cells”, Scientific Reports 3:1449-1461 (2013)

(152) A.B. Chambliss, P.-H. Wu, W.-C. Chen, S.X. Sun, and D. Wirtz, “Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution”, FASEB Journal 27:2667-2676 (2013)

(151) D.M Gilkes, S. Bajpai, P. Chaturvedi, D. Wirtz, and G.L. Semenza, “HIF-1 promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts”, Journal of Biological Chemistry 288: 10820-10829 (2013)

(150) D. Gilkes, S. Bajpai, C. C.-L. Wong, P. Chaturvedi, M.E. Hubbi, D. Wirtz, and G.L. Semenza, “Procollagen lysyl hydroxylase 2 is essential for hypoxia- induced breast cancer metastasis”, Molecular Cancer Research 11:456-466 (2013)

(149) D. Wirtz, “Interstitial friction greatly impacts membrane mechanics”, Biophysical Journal 104: 1217-1218 (2013)

(148) K. Konstantopoulos, P.H. Wu, and D. Wirtz, “Dimensional control of cancer cell migration”, Biophysical Journal 104: 279-280 (2013)

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(147) A.B. Chambliss, S.B. Khatau, P.-H. Wu, N. Erdenberger, D.K. Robinson, D. Hodzic, G.D. Longmore, and D. Wirtz, “The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction”, Scientific Reports (Nature) 3: 1087-1095 (2013)

(146) W.-C. Chen, P.-H. Wu, J.M. Phillip, S.B. Khatau, J.M. Choi, M.R. Dallas, K. Konstantopoulos, S.X. Sun, J.S.H. Lee, D. Hodzic, and D. Wirtz, “Functional interplay between cell cycle and cell phenotypes”, Integrative Biology 5: 523-534 (2013)

(145) D.-H. Kim and D. Wirtz, “Focal adhesion size uniquely predicts cell migration”, FASEB Journal 27: 1351-61 (2012)

(144) E. Chau, J.F. Galloway, A. Nelson, P.N. Breysse, D. Wirtz, P.C. Searson, and V.K. Sidhaye, “Effect of modifying quantum dot surface charge on airway epithelial cell uptake in vitro”, Nanotoxicology 7:1143-1151 (2012)

(143) K.L. Yap, S.I. Fraley, M.M. Thiaville, N. Jinawath, K. Nakayama, J.-L. Wang, T.-L. Wang, D. Wirtz, and I.-M. Shih, “NAC1 is an actin-binding protein that is essential for effective cytokinesis in cancer cells”, Cancer Research 72: 4085–4096 (2012)

(142) P.S. Raman, C.S. Alves, D. Wirtz, and K. Konstantopoulos, “Distinct Kinetic and molecular requirements govern CD44 binding to hyaluronan versus fibrin(ogen)”, Biophysical Journal 103: 415–423 (2012)

(141) D.H. Kim, S.B. Khatau, Y. Feng, S. Walcott, S.X. Sun, G.D. Longmore, and D. Wirtz, “Actin cap associated focal adhesions and their distinct role in cellular mechanosensing”, Scientific Reports (Nature) 2:555-568 (2012)

(140) S.B. Khatau, R.J. Bloom, S. Bajpai, D. Razafsky, S. Zang, A. Giri, P.-H. Wu, J. Marchand, A. Celedon, C.M. Hale, S.X. Sun, D. Hodzic, and D. Wirtz, “The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration”, Scientific Report (Nature) 2: 488-499 (2012)

(139) M.-H. Lee, P.-H. Wu, J.R. Staunton, R. Ros, G.D. Longmore, and D. Wirtz “Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer”, Biophysical Journal 102: 2731-41 (2012)

(138) S.B. Khatau, S. Kusuma, D. Hanjaya-Putra, P. Mali, L. Cheng, J.S.H. Lee, S. Gerecht, and D. Wirtz, “The differential formation of the LINC-mediated perinuclear actin cap in pluripotent and somatic cells”, PLOS One, e36689 (2012)

(137) M.R. Dallas, G. Liu, W.-C. Chen, S.N. Thomas, D. Wirtz, D.L. Huso, K. Konstantopoulos, "Divergent roles of CD44 and carcinoembryonic antigen in colon carcinoma metastasis", FASEB Journal 16: 2648-56 (2012)

(136) S.I. Fraley, Y. Feng, G.D. Longmore, and D. Wirtz, "Dimensional and temporal controls of cell migration by zyxin and binding partners in three-dimensional matrix”, Nature Communications 3:719-731 (2012)

(135) L.S.-L. Cheung, P.S. Raman, D. Wirtz and K. Konstantopoulos, “Biophysics of selectin-mediated cell–cell adhesion”, Comprehensive biophysics, Cell Press, Elsevier (2012)

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(134) D. Wirtz, “Biophysics of molecular cell mechanics”, Chapter 7, Comprehensive biophysics, Cell Press, Elsevier (2012)

(133) D. Wirtz, Cell Biophysics, Introduction, Comprehensive Biophysics, Elsevier (2012)

(132) P.-H. Wu, C.M. Hale, J.S.H. Lee, Y. Tseng, and D. Wirtz, “High-throughput ballistic injection nanorheology (htBIN) to measure cell mechanics”, Nature Protocols 7: 155–170 (2012)

(131) C.M. Hale, W.-C. Chen, S.B. Khatau, B.R. Daniels, J.S.H. Lee, and D. Wirtz, “SMRT analysis of MTOC and nuclear positioning reveals the role of EB1 and LIC1 in single-cell polarization”, Journal of Cell Science 124: 4267-4285 (2011)

(130) S. Walcott, D.-H. Kim, D. Wirtz, S.X. Sun, “Nucleation and decay initiation are the stiffness-sensitive phases of focal adhesion maturation, Biophysical Journal 101: 2919-2929 (2011)

(129) A. Celedon, C.M. Hale, and D. Wirtz, “Magnetic manipulation of nanorods in the nucleus of living cells”, Biophysical Journal 101 1880-1886 (2011)

(128) C.C.-L Wong, D. Gilkes, H. Zhang, J. Chen, H. Wei, P. Chaturvedi, S.I. Fraley, C.M. Wong U.-S. Khoo, I.O.L. Ng, D. Wirtz, and G.L. Semenza, “Hypoxia-Inducible Factor 1 is a master regulator of breast cancer metastatic niche formation" Proceedings of the National Academy of Sciences 108: 16369-13374 (2011)

(127) D. Wirtz, K. Konstantopoulos, and P.C. Searson, “Physics of cancer: the role of physical interactions and mechanical forces in cancer metastasis”, Nature Reviews Cancer 11: 512-522 (2011)

(126) D.H. Kim and D. Wirtz, "Recapitulating cancer cell invasion in vitro", Proceedings of the National Academy of Sciences 108: 6693-6694 (2011)

(125) S.I. Fraley, C.M. Hale, R.J. Bloom, A. Celedon, J.S.H. Lee, and D. Wirtz, “Intra- and extracellular microrheology of endothelial cells in a 3D matrix”, Biophysical Regulation of Vascular Differentiation and Assembly; Biological and Medical Physics, Biomedical Engineering, Springer Verlag (2011)

(124) P.S. Raman, C.S. Alves, D. Wirtz, K. Konstantopoulos, “Single molecule binding of CD44 to fibrin versus P-selectin predicts their distinct shear-dependent interactions in cancer, Journal of Cell Science 124: 1904-1910 (2011)

(123) J.S.H. Lee and D. Wirtz, “Physical Oncology”, Preface, Physical Biology (2011) (122) L.S.-L. Cheung, P.S. Raman, E.M. Balzer, D. Wirtz, and K. Konstantopoulos,

“Biophysics of selectin-ligand interactions in inflammation and cancer”, Physical Biology 8: 015013 (2011)

(121) S. I. Fraley, Y. Feng, D. Wirtz and G.D. Longmore, “Reply: reducing background fluorescence reveals adhesions in 3D matrices”, Nature Cell Biology 13: 5-7 (2011)

(120) A. Celedon, D. Wirtz, and Sean X. Sun, “Torsional mechanics of DNA are regulated by small-molecule intercalation”, Journal of Physical Chemistry B 114: 16929-16935 (2010)

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(119) T.M. Dobrowsky and D. Wirtz, “Single-molecule analysis of cell-virus binding interactions”, Cell Signalling Reactions: Single-Molecular Kinetic Analysis, Springer Verlag, 153-166 (2010)

(118) B.R. Daniels, C.M. Hale, S.B. Khatau, S. Kusuma, T.M. Dobrowsky, S. Gerecht, and D. Wirtz, “The difference in microrheology between human induced pluripotent stem (iPS) cells and human embryonic stem (hES) cells”, Biophysical Journal 99: 3563-3570 (2010)

(117) P. Mali, D. Wirtz, and P.C. Searson, “Interplay of RhoA and motility in the programmed spreading of daughter cells post mitosis”, Biophysical Journal 99: 3526-3534 (2010)

(116) D. Wirtz and S.B. Khatau, “Bundles from boundaries”, Nature Materials 9: 788-790 (2010)

(115) A. Dajkovic, S. Pichoff, J. Lutkenhaus, and D Wirtz, “Cross-linking FtsZ polymers into coherent Z rings”, Molecular Microbiology 78, 651–668 (2010)

(114) P.J. Stahl, N.H. Romano, D. Wirtz, and S.M. Yu, “PEG-based hydrogels with collagen mimetic peptide-mediated and tunable physical cross-links”, Biomacromolecules 11: 2336–2344 (2010).

(113) B.R. Daniels, T.M. Dobrowsky, E.M. Perkins, S.X. Sun, and D. Wirtz, “MEX-5 enrichment in C. elegans early embryos mediated by differential diffusion”, Development 137: 2579-2585 (2010)

(112) S. I. Fraley, Y. Feng, D.H. Kim, A. Celedon, G.D. Longmore, and D. Wirtz, “The distinctive role of focal adhesion proteins in three-dimensional cell motility”, Nature Cell Biology 12: 598-604 (2010)

(111) T.M. Dobrowsky, B.R. Daniels, R.F. Siliciano, S.X. Sun, and D. Wirtz, “Organization of cellular receptors into a nanoscale junction during HIV-1 adhesion”, PLOS Computational Biology 6: e1000855 (2010)

(110) B. Wildt, D. Wirtz*, and P.C. Searson, “Triggering cell detachment from patterned electrode arrays by programmed subcellular release”, Nature Protocols 5: 1273-1280 (2010) (*=corresponding author)

(109) S.B. Khatau, D.H. Kim, C.M. Hale, and D. Wirtz, “The perinuclear actin cap in health and disease”, Nucleus 1: 337-342 (2010).

(108) E. Esue, L. Ruppercht, D. Wirtz, “Dynamics of the bacterial intermediate filament crescentin in vitro and in vivo”, PLOS One e8855 (2010)

(107) S.B. Khatau, C.M. Hale, P.J. Stewart-Hutchinson, M.S. Patel, C.L. Stewart, P.C. Searson, D. Hodzic, and D Wirtz, “A perinuclear actin cap regulates nuclear shape”, Proceedings of the National Academy of Sciences 106: 19017-19022 (2009)

(106) C.M. Hale, S.X. Sun, and D. Wirtz, “Resolving the role of actomyosin contractility in cell microrheology”, PLOS One e7054 (2009)

(105) J.F. Galloway, J. Park, K.H. Lee, D. Wirtz, and P.C. Searson, “Exploiting nucleation and growth in the synthesis and electrical passivation of CdSe quantum dots”, Science of Advanced Materials 1: 93-100 (2009)

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(104) S. Bajpai, Y. Feng, R. Krishnamurthy, G.D. Longmore, and D. Wirtz, “Loss of α-catenin decreases the strength of single E-cadherin bonds between human cancer cells”, Journal of Biological Chemistry 284: 18252-18259 (2009)

(103) D. Wirtz, “Particle tracking microrheology of living cells: principles and applications”, Annual Review of Biophysics 38: 301-326 (2009)

(102) A. Celedon, I.M. Nodelman, B. Wildt, R. Dewan, P.C. Searson, D. Wirtz, G. D. Bowman, and S.X. Sun, “Single molecule torque measurements using nanorods in magnetic tweezers”, Nano Letters 9: 1720-1725 (2009)

(101) B. Wildt, D. Wirtz*, and P.C. Searson*, “Programmed sub-cellular release for studying the dynamics of cell detachment”, Nature Methods 6: 211-213 (2009) (*=corresponding author)

(100) B.R. Daniels, E.M. Perkins, T.M. Dobrowsky, S.X. Sun, and D. Wirtz, "Asymmetric enrichment of PIE-1 in the C. elegans zygote is mediated by binary counter-diffusion", Journal of Cell Biology 184: 473-479 (2009)

(99) O. Esue, Y. Tseng, and D. Wirtz, “α-Actinin and filamin cooperatively enhance the stiffness of actin filament networks “, PLOS One 4: e4411 (2009).

(98) S. K. Lai, Y. Wang, R. Cone, D. Wirtz, and J. Hanes,, “Selectively altering mucus rheology to "solidify" human mucus at the nanoscale”, PLOS One 4: e4294 (2009)

(97) G. Lan, B.R. Daniels, T.M. Dobrowsky, D. Wirtz, and S.X. Sun, ”Condensation of FtsZ filaments drives bacterial cell division”, Proceedings of the National Academy of Sciences 106: 121-126 (2009)

(96) S.K. Lai, Y.-Y. Wang, D. Wirtz, J. Hanes, “Micro- and macrorheology of mucus Advanced Drug Delivery Reviews 61: 86-100(2008)

(95) M.S. Thompson and D. Wirtz, “Sensing cytoskeletal mechanics by ballistic intracellular nanorheology (BIN) coupled with cell transfection”, Methods in Cell Biology 89: 467-486 (2008)

(94) T.M. Dobrowsky, P. Panorchan, K. Konstantopoulos, and D. Wirtz, “Live-cell single-molecule force spectroscopy”, Methods in Cell Biology 89: 411-432 (2008)

(93) S. Bajpai, J. Correia, Y. Feng, J. Figueiredo, S.X. Sun, G.D. Longmore, G. Suriano, and D. Wirtz, “α-Catenin mediates initial E-cadherin-dependent cell-cell recognition and subsequent bond strengthening”, Proceedings of the National Academy of Sciences 105: 18331–18336 (2008)

(92) C.M. Hale, A.L. Shrestha, S.B. Khatau, P.J. Stewart-Hutchinson, L. Hernandez, C.L. Stewart, D. Hodzic, and D. Wirtz, “Dysfunctional connections between the nucleus and the actin and microtubule networks in laminopathic models”, Biophysical Journal 95: 5462–5475 (2008)

(91) O. Esue, E.S. Harris, H.N. Higgs, and D. Wirtz, “The F-actin crosslinking/bundling activity of mammalian formins”, Journal of Molecular Biology 384: 324 –334 (2008)

(90) G. Lan, A. Dajkovic, D. Wirtz, and S.X. Sun, “Polymerization and bundling kinetics of FtsZ filaments”, Biophysical Journal 95: 4045-4056 (2008)

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(89) R.J. Bloom, J. P. George, A. Celedon, S.X. Sun, and D. Wirtz, “Mapping local matrix remodeling induced by a migrating tumor cell using 3-D multiple-particle tracking”, Biophysical Journal 95: 4077-4088 (2008)

(88) T.M. Dobrowsky, Y. Zhou, S.X. Sun, R.F. Siliciano, and D. Wirtz, “Monitoring early fusion dynamics of human immunodeficiency virus type 1 at single-molecule resolution”, Journal of Virology 82: 7022–7033 (2008)

(87) X. Zhou et al., “Fibronectin fibrillogenesis regulates 3-dimensional neovessel formation”, Genes and Development 22: 1231-43 (2008)

(86) P.J. Stewart-Hutchinson, C.M Hale, D. Wirtz, and D. Hodzic, “Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness”, Experimental Cell Research 314: 1892-905 (2008)

(85) A. Dajkovic, G. Lan, S.X. Sun, D. Wirtz*, and J. Lutkenhaus, “MinC spatially controls bacterial cytokinesis by antagonizing the scaffolding function of FtsZ”, Current Biology 18: 235–244 (2008) (*=corresponding author)

(84) J.S.H. Lee, C.M. Hale, P. Panorchan, S.B. Khatau, J.P. George, C. L. Stewart, D. Hodzic, and D. Wirtz, “Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization and migration”, Biophysical Journal 93: 2542-2552 (2007)

(83) J.Y., Xu, T. Chang, G.E. Inglett, S. Kim, Y. Tseng, and D. Wirtz “Micro-heterogeneity and micro-rheological properties of high-viscosity oat beta-glucan solutions”, Food Chemistry 103: 1192-1198 (2007)

(82) P. Panorchan, J.S.H. Lee, T.P. Kole, Y. Tseng, and D. Wirtz, “Probing cell mechanics responses to stimuli using ballistic intracellular nanorheology”, Methods in Cell Biology 83: 113-140 (2007)

(81) O. Esue, A.A. Carson, Y. Tseng, and D. Wirtz, “A direct interaction between actin and vimentin filaments mediated by the tail domain of vimentin”, Journal of Biological Chemistry 281: 30393-30399 (2006)

(80) P. Panorchan, J.S.H. Lee, T.P. Kole, Y. Tseng, and D. Wirtz, “Microrheology and ROCK signaling of human endothelial cells embedded in a 3D matrix”, Biophysical Journal 91: 3499-3507 (2006)

(79) B.R. Daniels, B.C. Masi, and D. Wirtz, “Probing single-cell micromechanics in vivo: the microrheology of C. elegans developing embryos”, Biophysical Journal 90: 4712-4719 (2006)

(78) P. Panorcham, J.P. George, and D. Wirtz, “Probing intercellular interactions between vascular endothelial cadherin pairs at single-molecule resolution and in living cells” Journal of Molecular Biology 358: 665-674 (2006)

(77) J. S.H. Lee, P. Panorchan, C.M. Hale, S.B. Khatau, Y. Tseng, and D. Wirtz, “Ballistic intracellular nanorheology reveals ROCK-hard stiffening response in to fluid flow”, Journal of Cell Science 119: 1760-1768 (2006)

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(76) O. Esue, D. Wirtz, and Y. Tseng, “GTPase activity, structure and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB”, Journal of Bacteriology 188: 968-976 (2006)

(75) P. Panorchan, M. S. Thompson, K. J. Davis, Y. Tseng, K. Konstantopoulos, and D. Wirtz, “Single-molecule analysis of cadherin-mediated cell-cell adhesion”, Journal of Cell Science 119: 66-73 (2006)

(74) S. X. Sun and D. Wirtz, “Mechanics of enveloped virus entry into host cell”, Biophysical Journal 90: L10-L12 (2006)

(73) E. Atilgan, D. Wirtz, and S. X. Sun, “Mechanics and dynamics of actin-driven thin protrusions”, Biophysical Journal 90: 65-76 (2006)

(72) M.I. Chang, P. Panorchan, T. M. Dobrowsky, Y. Tseng, and D. Wirtz, “Single-molecule analysis of HIV-1 gp120-receptor interactions in living cells”, Journal of Virology 79: 14748-14755 (2005)

(71) E. Atilgan, D. Wirtz and S. X. Sun, “Morphology of the lamellipodium and the organization of actin at the leading edge of crawling cells”, Biophysical Journal 89:3589-3602 (2005)

(70) O. Esue, Y. Tseng, and D. Wirtz, “Mechanical shear can accelerate the gelation of actin filament networks”, Physical Review Letters 95: 048301 (2005)

(69) Y. Tseng, T. P. Kole, J. S. H. Lee, E. Fedorov, S. C. Almo, B. W. Schafer, and D. Wirtz, “How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response”, Biochemical and Biophysical Research Communications 334: 183-192 (2005)

(68) O. Esue, Y. Tseng, and D. Wirtz, “The rapid onset of elasticity during the assembly of the bacterial cell-division protein FtsZ”, Biochemical and Biophysical Research Communications 333: 508-516 (2005)

(67) S.L. Gupton, R.S. Fischer, T.P. Kole, A. Ponti, G. Danuser, S. E. Hitchcock-DeGregori, V.M. Fowler, D. Wirtz, D. Hanein, and C.M. Waterman-Storer, “Cell migration without a lamellipodium: Translation of actin dynamics into cell movement mediated by tropomyosin”, Journal of Cell Biology 168:619-631 (2005)

(66) J.S.H. Lee, Y. Tseng, and D. Wirtz, “Cdc42 mediates nucleus movement and MTOC polarization in Swiss 3T3 fibroblasts under shear”, Molecular Biology of the Cell 16: 871-880 (2005)

(65) O. Esue, M. Cordero, Y. Tseng, and D. Wirtz, “The assembly of MreB, a prokaryotic homolog of actin", Journal of Biological Chemistry 280: 2628-2635 (2005)

(64) T.P. Kole, Y. Tseng, J.L. Katz, and D. Wirtz, “Intracellular mechanics of migrating fibroblasts”, Molecular Biology of the Cell 16: 328-338 (2005)

(63) T.P. Kole, Y. Tseng, and D. Wirtz, “Intracellular microrheology as a tool for the measurement of the local mechanical properties of live cells”, Methods in Cell Biology 79: 48-64 (2004)

(62) Y. Tseng and D. Wirtz, “Dendritic branching and homogenization of actin network mediated by Arp2/3 complex”, Physical Review Letters 93: 258104 (2004)

(61) P. Panorchan, D. Wirtz, and Y. Tseng, “Structure-function relation of biological gels

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revealed by multiple-particle tracking and DIC microscopy: the case of human lamin networks”, Physical Review E 70: 041906 (2004)

(60) P. Panorchan, B. W. Schafer, D. Wirtz, and Y. Tseng, “Nuclear envelope breakdown requires overcoming the mechanical integrity of the nuclear lamina”, Journal of Biological Chemistry 278:43462-43467 (2004)

(59) M.G. Klein, W. Shi, Y. Tseng, D. Wirtz, D.R. Kovar, C.J. Straiger, and S.C. Almo, “Structure of the actin-crosslinking core of fimbrin”, Structure 12: 999-1013 (2004)

(58) T.P. Kole, Y. Tseng, L. Huang, J.L. Katz, and D. Wirtz, “Rho kinase regulates the micromechanical response of adherent cells to Rho activation”, Molecular Biology of the Cell 15: 3475-3484 (2004)

(57) W.D. Hanley, D. Wirtz*, and K. Konstantopoulos*, “Distinct kinetic and mechanical properties govern selectin-leukocyte interactions, Journal of Cell Science 117: 2503-2511 (2004) (*=corresponding authors)

(56) M. Dawson, E. Krauland, D. Wirtz, and J. Hanes, “Transport of polymeric nanoparticles gene carriers in gastric mucus”, Biotechnology Progress 20: 851-857 (2004)

(55) Y. Tseng, J.S.H. Lee, T.P. Kole, I. Jiang, and D. Wirtz, “Micro-organization and visco-elasticity of the nucleus revealed by particle nanotracking”, Journal of Cell Science 117: 2159-2167 (2004)

(54) S.R. Heidemann and D. Wirtz, “Towards a regional approach to cell mechanics”, Trends in Cell Biology 14: 160-166 (2004)

(53) J. Suh, D. Wirtz*, and J. Hanes*, “Real-time intracellular transport phenomena of gene nanocarriers studied by multiple particle tracking”, Biotechnology Progress 20: 598-602 (2004)

(52) Y. Tseng, K.M. An, O. Esue, and D. Wirtz “The bimodal role of filamin in controlling the architecture and mechanics F-actin networks”, Journal of Biological Chemistry (2004) 279: 1819-1826

(51) M. Dawson, D. Wirtz*, and J. Hanes*, “Enhanced viscoelasticity of human cystic fibrotic sputum correlates with increasing microheterogeneity in particle transport”, Journal of Biological Chemistry 278: 50393-50401 (2003) (*=corresponding authors)

(50) K. Konstantopoulos, W. Hanley, and D. Wirtz, “Receptor/ligand binding: “catch” bonds finally captured experimentally”, Current Biology 13: R611-615 (2003)

(49) Y. Zhou, D. Wirtz*, and G. Chirikjian*, “Determining the Gaussian probability distribution of the best-fit ellipsoid of revolution for a polymer chain from planar projections”, Inverse Problems 19: 725-738 (2003)(*=corresponding authors)

(48) S. Yamada, D. Wirtz*, and P.A. Coulombe*, “The mechanical properties of simple epithelial keratin 8 and 18: Discriminating between interfacial and bulk elasticities”, Journal of Structural Biology 143: 45-55 (2003) (*=corresponding authors)

(47) W. Hanley, O. McCarty, S. Jadhav, Y. Tseng, D. Wirtz*, and K. Konstantopoulos*, “Single-molecule characterization of P-selectin/ligand binding”, Journal of Biological Chemistry 278: 10556-10561 (2003) (*=corresponding authors)

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(46) H.Y. Li, D. Wirtz, and Y. Zheng, “A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo”, Journal of Cell Biology 160: 635-644 (2003)

(45) J. Suh, D. Wirtz*, and J. Hanes*, “Effective active transport of gene nanocarriers to the cell nucleus”, Proceedings of the National Academy of Sciences USA 100: 3878-3882 (2003) (*=corresponding authors)

(44) Y. Tseng, T. P. Kole, and D. Wirtz, “Micromechanical mapping of live cells by multiple particle tracking microrheology”, Biophysical Journal 83: 3162-3176 (2002)

(43) Y. Tseng, T. P. Kole, S.-H. J. Lee, and D. Wirtz, “Local dynamics and viscoelastic properties of cell biological systems”, Current Opinion in Colloid & Interface Science 7: 210-217 (2002)

(42) A. Goodman, Y. Tseng, and D. Wirtz, “Effect of length, topology, and concentration on the microviscosity and microheterogeneity of DNA solutions”, Journal of Molecular Biology 323: 199-215 (2002)

(41) A. Rahman, Y. Tseng, and D. Wirtz, “Micromechanical coupling between surface cell receptors and RGD peptides”, Biochemical and Biophysical Research Comm. 296: 771-778 (2002)

(40) Y. Tseng, B.W. Schafer, S.C. Almo, and D. Wirtz, “Functional synergy of actin filament crosslinking proteins”, Journal of Biological Chemistry 277: 25609-25616 (2002)

(39) Y. Tseng, K.M. An, and D. Wirtz, “Microheterogeneity controls the rate of gelation of actin filament networks”, Journal of Biological Chemistry 277:18143-18150 (2002)

(38) J. Xu, Y. Tseng, C.J. Carriere, and D. Wirtz, “Microheterogeneity and microrheology of wheat gliadin suspensions studied by multiple particle tracking”, Biomacromolecules 3: 92-99 (2002)

(37) S. Yamada, D. Wirtz, and P.A. Coulombe, “Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments”, Molecular Biology of the Cell 13: 382-391 (2002)

(36) O. Bousquet, L. Ma, S. Yamada, C. Gu, T. Idei, K. Takahashi, D. Wirtz, and P.A. Coulombe, “The non-helical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro”, Journal of Cell Biology 155: 747-754 (2001)

(35) Y. Tseng and D. Wirtz, “Mechanics and multiple particle tracking micro-heterogeneity of α-actinin crosslinked actin filament networks”, Biophysical Journal 81: 1643-1656 (2001)

(34) Y. Tseng, E. Fedorov, M. McCaffery, S.C. Almo, and D. Wirtz, “Micromechanics and ultrastructure of actin filament networks crosslinked by fascin: A comparison with α-actinin”, Journal of Molecular Biology 310: 351-366 (2001)

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(33) L. Ma, S. Yamada, D. Wirtz, and P.A. Coulombe, “A "hot-spot" mutation alters the mechanical properties of keratin filament networks”, Nature Cell Biology 3: 503-506 (2001)

(32) J. Xu, J.A. Bietz, F.C. Felker, and D. Wirtz, “Rheological properties of vital wheat gluten suspensions”, Cereal Chemistry 78: 181-185 (2001)

(31) C. Haber and D. Wirtz, “Magnetic tweezers for DNA micromanipulation”, Review of Scientific Instruments 71: 4561-4570 (2000)

(30) P.A. Coulombe, O. Bousquet, L. Ma, S. Yamada, and D. Wirtz, “The "ins" and "outs" of intermediate filament organization”, Trends in Cell Biology 10: 420-428 (2000)

(29) J. Xu, Y. Tseng, and D. Wirtz, “Strain-hardening of actin filament networks: regulation by the dynamic crosslinking protein α-actinin”, Journal of Biological Chemistry 275: 35886-35892 (2000)

(28) C. Haber, S.A. Ruiz, and D. Wirtz, “Shape anisotropy of a single random-walk polymer”, Proceedings of the National Academy of Sciences USA 97: 10792-10795 (2000)

(27) C. Haber and D. Wirtz, “Shear-induced λ-phage DNA assembly”, Biophysical Journal 79: 1530-1536 (2000)

(26) J. Apgar, Y. Tseng, E. Fedorov, M.B. Herwig, S.C. Almo, D. Wirtz, “Multiple-particle tracking measurements of the heterogeneities of solutions of actin filaments and actin bundles”, Biophysical Journal 79: 1095-1106 (2000)

(25) S. Yamada, D. Wirtz, and S.C. Kuo, “Mechanics of living cells measured by laser tracking microrheology (LTM)”, Biophysical Journal 78: 1736-1747 (2000)

(24) L. Ma, J. Xu, P.A. Coulombe, and D. Wirtz, “Epidermal keratin suspensions show unique micromechanical properties”, Journal of Biological Chemistry 274: 19145-19151 (1999)

(23) P. Leduc, C. Haber, G. Bao, and D. Wirtz, “Dynamics of individual flexible polymers in a shear flow”, Nature 399: 564-566 (1999)

(22) A. Palmer, J. Xu, S.C. Kuo, and D. Wirtz, “Diffusing wave spectroscopy microrheology of actin filament networks”, Biophysical Journal 76: 1063-1071 (1999)

(21) K. Rufener, A. Palmer, J. Xu, and D. Wirtz, “High-frequency dynamics and microrheology of macromolecular solutions measured by diffusing wave spectroscopy: the case of actin filament networks”, Journal of Non-Newtonian Fluid Mechanics 82: 303-314 (1999)

(20) J. Xu, D. Wirtz, and T.D. Pollard, “Dynamic crosslinking by α-actinin determines the mechanical properties of actin filament networks”, Journal of Biological Chemistry 273: 9570-9576 (1998)

(19) A. Palmer, B. Cha, and D. Wirtz, “Effect of Latrunculin A on the rheology of actin filament networks”, Journal of Polymer Science. B: Phys. Ed. 36: 3007-3015 (1998)

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(18) J. Xu, A. Palmer, and D. Wirtz, “Rheology and microrheology of semiflexible polymer solutions”, Macromolecules 31: 6486-6492 (1998)

(17) W. Petka, J.H. Harden, J. McGrath, D. Wirtz, and D.A. Tirrell, “Reversible hydrogels from self-assembling artificial proteins”, Science 281: 389-392 (1998)

(16) A. Silva and D. Wirtz “Dominant mode of diffusion in the self-similar phase separation of a magnetic fluid”, Langmuir 14: 578-581 (1998)

(15) J. Xu, V. Viasnoff, and D. Wirtz, “Compliance of actin filament networks measured by diffusing wave spectroscopy”, Rheologica Acta 37: 387-398 (1998)

(14) A. Palmer, J. Xu, and D. Wirtz, “High-frequency viscoelasticity of crosslinked actin filament networks measured by diffusing wave spectroscopy”, Rheologica Acta 37: 97-106 (1998)

(13) T.G. Mason, A. Dhople, and D. Wirtz, “Linear viscoelastic moduli of concentrated DNA solutions”, Macromolecules 31: 3600-3603 (1998)

(12) T.G. Mason, A. Dhople, and D. Wirtz, “Concentrated DNA rheology and microrheology”, MRS Proceedings 463: 153-158 (1997)

(11) T.G. Mason, K. Ganesan, J. van Zanten, D. Wirtz, S.C. Kuo, “Particle-tracking microrheology of complex fluids”, Physical Review Letters 79: 3282-3285 (1997)

(10) A. Silva, Bond, R., Plouraboue, F., and Wirtz, D., “The fluctuation dynamics of a single magnetic chain”, Physical Review E 54: 5502-5510 (1996)

(9) G.G. Fuller, J.W. van Egmond, D. Wirtz, E. Peuvrel-Didier, E. Wheeler, and H. Takahashi, “Enhancement of concentration fluctuations by external fields”, ACS Symposium Series 597: 22-34 (1995)

(8) D. Wirtz, “Direct measurement of the transport properties of a single DNA molecule”, Physical Review Letters 75: 2236-2439 (1995)

(7) D. Wirtz and M. Fermigier, “Periodic structures and substructures in magnetic suspensions” Langmuir 11: 398-400 (1995)

(6) D. Wirtz and M. Fermigier, “One-dimensional patterns and wavelength selection in magnetic fluids”, Physical Review Letters 72: 2294-2297 (1994)

(5) D. Wirtz, “Flow-induced scattering peak in the structure factor of polymer solutions”, Physical Review E 50: R1755-R1758 (1994)

(4) D. Wirtz, “Fluctuation enhancement and reduction in sheared polymer solutions”, Macromolecules 27: 5639-5642 (1994)

(3) D. Wirtz, D.E. Werner, and G.G. Fuller, “Structure and optical anisotropies of critical polymer solutions in electric fields”, Journal of Chemical Physics 101: 1679-1686 (1994)

(2) D. Wirtz and G.G. Fuller, “Phase transitions induced by electric fields in near-critical polymer solutions”, Physical Review Letters 71: 2236-2239 (1993)

(1) D. Wirtz, K. Berend, and G.G. Fuller, “Electric-field-induced structure in polymer solutions near the critical point”, Macromolecules 25: 7234-7246 (1992)

Book/Special Issue

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(4) D. Wirtz, Comprehensive Biophysics, “Cell Biophysics”, Elsevier B.V. (2012) (3) J.S.H. Lee and D. Wirtz, Guest Editors, “Physical Oncology”, Physical Biology 8,

February issue (2011) (2) D. Wirtz and E. Evans, Guest Editors, “Materials Science of the Cell”, MRS Bulletin

24, October issue (1999) (1) D. Wirtz and T.C. Halsey, Editors of “Statistical Mechanics in Physics and Biology”,

MRS vol 254 (1997) VIII. FUNDING Ongoing Research Support T32CA153952 (Wirtz) 09/01/15 – 08/31/20 NIH/NCI T32: Predoctoral and Postdoctoral Training Program in Nanotechnology for Cancer Research. Objective: This grant will support an integrated interdisciplinary predoctoral and postdoctoral training program in nanotechnology for cancer medicine. DBI-1460716 (Wirtz) 02/01/15 – 01/31/18 NSF REU – Research Experiences for Undergraduates: Site program in Nanotechnology for biology and bioengineering Objective: The major goal is to provide undergraduate students, including high school students, with challenging research experiences in the exciting and rapidly growing area of nanotechnology. R01CA174388 (Wirtz) 09/01/12 – 06/31/17 NIH/NCI Single-cell phenotyping for therapeutic stratification in pancreatic cancer Objective: Use a recently developed high throughput automated platform high-throughput cell phenotyping htCP assay, which can assess cellular phenotypes and ascribe accurate predictive and prognostic parameters to human cancer specimens. R25CA153952 (Wirtz) 09/03/10 – 07/31/16 NIH/NCI The Johns Hopkins Cancer Nanotechnology Training Center Objective: To create a predoctoral training program in nanotechnology for cancer medicine that will be positioned at this interface and will establish a unique national resource with interdisciplinary and synergistic programs in fundamental cancer biology and translational research in cancer medicine. MCB-1157687 (Hristova/Wirtz) 07/08/04 – 06/30/16 NSF Lateral Interactions between Proteins in Membranes Objective: to test the biological relevance of cadherin TM domain interactions by measuring the adhesion properties of cadherins with TM domains that are mutated to have altered lateral interactions. Recently Completed Research Support DBI-1062975 (Wirtz) 02/15/11 – 01/31/16 NSF

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REU: Site program in Nanotechnology for biology and bioengineering Objective: The major goal is to provide undergraduate students with challenging research experiences in the exciting and rapidly growing area of nanotechnology. U54CA143868 (Wirtz) 09/28/09 – 01/31/15 NIH/NCI Center for Cancer Physics Objective: The Center will focus its efforts on establishing molecular and cellular mechanisms by which cancer cells respond to mechanical forces during metastasis T32CA130840 (Wirtz) 07/01/08 – 06/30/14 NIH/NCI T-32 NIH Postdoctoral Training Program in Nanotechnology for Cancer Medicine NTCM Objective: To create a postdoctoral training program in nanotechnology for cancer medicine (NTCM) that will be positioned at this interface and will establish a unique national resource with interdisciplinary and synergistic programs in fundamental research in cancer biology, industrial collaboration, and translational research in cancer medicine. IX. RESEARCH SUPERVISED

Number of PhD students placed in tenure-track/tenured positions: 9 (UC Davis, Ohio State University, Rice University, Brown University, Carnegie Mellon University, UC San Diego (2), Korea University, IIT Madras)

Number of postdocs placed in tenure-track/tenured positions: 4 (UCLA, University of Ottawa, University of Florida, Johns Hopkins University,) Current PhD students (5): (1) Nicolas Perez (Catolica University, Santiago, Chile, 2014-present) (2) Michelle Karl (North Dakota State U., 2016-present) (3) Alexandra Sneider (Harvard U, 2017-present)

Current Postdoctoral Fellows (4) (1) Pei-Hsun Lee, PhD (U. Florida; 2010-present) (2) Meng Horn Lee, PhD (SUNY Buffalo, 2010-present) Past PhD students (29) Hasini Jayatilaka (Johns Hopkins University, 2012-2016), Current position:

Postdoctoral fellow, Stanford University School of Medicine, Stanford, CA (28) Jacob Sarnecki (Univ. Maryland Baltimore County, 2012-2015), Current

position: , MD (27) Ivie Aifuwa (SUNY Buffalo, 2011-2015), Current position: Engineer, Seattle,

WA (26) Jude Phillip (City College of New York, 2010-2015). Current position:

Postdoctoral fellow, Weil Cornell University Medical School, New York City, NY. (25) Sarita Koride (IIT, Calcutta, 2010-2015). Current position: Postdoctoral

researcher, Pfizer.

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(24) Angela Jimenez (City College of New York, 2009-2015). Current position: Postdoctoral fellow, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD.

(23) Anjil Giri (University of Idaho, 2009-2014). Current position: Development Scientist, Alexion Pharmaceuticals, NY.

(22) Allison Chambliss (Virginia Tech. University, 2009-2014). Current position: Postdoctoral fellow, Johns Hopkins Hospital, MD.

(21) Wei-Chiang Chen (University of Florida, 2009-2014). Current position: Scientist, ImmunoGen, Inc., MA.

(20) Dong-Hwee Kim (University of Seoul, 2008-2012). Thesis topic: “Biophysics of the actin cap”. Current position: Assistant Professor, Korea University, Korea.

(19) Stephanie M. Fraley (University of Kentucky; 2009-2012). NSF fellow. Siebel Award. Current position: Assistant Professor, UC San Diego, CA.

(18) Shyam B. Khatau (Johns Hopkins University; 2008-2011). NSF-IGERT Fellow. Thesis topic: “Molecular regulation of nuclear shape in health and disease.” Current position: Managing Consultant, Navigant, IL.

(17) Matthew Keuss, (University of Missouri, 2006-2012). HHMI fellow. Thesis topic: “Biophysics of neuron dynamics.” Current position: Postdoctoral fellow, University of Dundee, Scotland.

(16) Ryan Braun (University of Minnesota; 2002-2011). Thesis topic: “Multiple-particle tracking of soft materials”. Current position: Advanced Manufacturing Technology Specialist, 3M, MN.

(15) Saumendra Bajpai (IIT Calcutta, India, 2006-2011). Thesis topic: “Single-molecule analysis of cadherin-mediated cell-cell adhesion.” Current position: Assistant Professor, IIT Madras, India.

(14) Christopher M. Hale (Johns Hopkins University; 2006-2011). Thesis topic: “Biophysical approaches to the problem of aging and laminopathies”. Current position: Scientist, Amgen, CA.

(13) Terrence M. Dobrowsky (Notre Dame University, 2004-2010). HHMI fellow. Thesis topic: “Single-molecule analysis of HIV entry”. Current position: Senior Engineer, Biogen Idec, MA.

(12) Brian R. Daniels, (Johns Hopkins University; 2003-2009). Thesis topic: “Molecular control of embryonic cell polarization”. AAAS Fellow. Current position: Alliance Manager, Broad Institute, MA.

(11) Melissa S. Thompson, (North Carolina State University; 2003-2008). Thesis topic: “Role of GTPases in ovarian cancer motility and invasion”. Current position: Associate Director, University of Texas Health Science Center, TX.

(10) Jerry S.H. Lee (Johns Hopkins University; 2002-2006). Thesis topic: “Role of small GTPases in cell polarization”, Current position: Health Sciences Director, National Cancer Institute, NIH, MD.

(9) Porntula “Paul” Panorchan (University of Delaware; 2002-2006). Thesis topic: “The structural function of lamin in health and disease”, Current position: Director, Vertex Pharmaceuticals, MA.

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(8) Osigwe Esue (University of Maryland BC; 2001-2006). Thesis topic: “The role of cytoskeleton proteins in determining cell shape and migration”, Current position: Senior Manager, Genentech, CA.

(7) Michelle Dawson, (Johns Hopkins, 2000-2005). Co-advised with Prof. J. Hanes. Current position: Assistant Professor, Department of Chemical & Biomolecular Engineering, Georgia Institute of Technology, GA.

(6) Junghae Suh (Johns Hopkins, 1999-2004), Co-advised with Prof. J. Hanes. Current position: Associate Professor, Department of Bioengineering, Rice University, TX.

(5) Thomas P. Kole (Johns Hopkins; 1999-2005). Thesis topic: “Cell mechanics and motility and the activation of small GTPases”. Current position: Attending Radiation Oncologist, Winthrop-University Hospital, NY.

(4) Soichiro Yamada (Rutgers University; 1997-2001). Thesis topic: “Microrheology of cytoskeleton: F-actin and keratin filaments”. Current position: Associate Professor, Department of Biomedical Engineering, UC Davis, CA.

(3) Charbel Haber (Johns Hopkins; 1995-1999). Thesis topic: “Magnetic tweezers: micromanipulation of DNA molecules”. Current position: Head, Global Regulatory Affairs – Immunology & Neurology, EMD Serano, Inc., MA.

(2) Adriana Silva (University of Rio de Janeiro; Ph.D. 1995-1999). Thesis topic: “Kinetics and dynamics of magnetic suspensions under applied magnetic fields”. Current position: Staff Engineer, ExxonMobil Chemical Co., TX.

(1) Andre F. Palmer (Howard University; Ph.D. 1995-1998). Thesis topic: “Structure and kinetics of actin networks in the presence of auxiliary proteins”. Current position: Professor and Chair, Department of Chemical & Biomolecular Engineering, Ohio State University, OH. Recipient of an NSF CAREER Award

Past Master Students (6) Jenna Graham (Catholic University, 2012-present), PhD Student, ETH Zurich (5) Nitish Gandhi (IIT Calcutta, India, 2009-2011). (4) Karen Leslie (Washington University; 2001-2003). Thesis topic: “In vitro models

of angiogenesis”. Current position: General Manager, Viva Generik, Indonesia. (3) Matthew B. Herwig (University of Maryland BC; 2000). Thesis topic: “Role of

focal adhesion molecules in cell morphology, adhesion, and survival”. Current position: Senior Project Manager, Medimmune, MD.

(2) Karl Rufener (University of Illinois; M.Sc. 1998, co-Advisor: J. van Zanten). Thesis topic: “Diffusing wave spectroscopy and the rheology of a model wormlike micelle system”. Current position: Research Engineer, LyondellBasell, PA.

(1) Robert Bond (University of Pennsylvania; M.Sc., 1996). Thesis topic: “An experimental study of the kinetics of aggregation in magnetic suspensions”. Current position: Solutions Architect/ Pre-Sales, Qlik, PA.

Past Postdoctoral Fellows / Associate Research Scientists (9) Dr. Lijuan He (Johns Hopkins University; 2011-2015), Patent reviewer, CDC, MD (8) Dr. Yu-Tai Chang (UC Davis; 2013-2014); postdoctoral fellow, Johns Hopkins

School of Medicine, Baltimore, MD

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(7) Dr. Daniele Gilkes, PhD (Moffit Cancer Center; 2009-2014). Current position: Assistant Professor, Department of Oncology, The Johns Hopkins University, MD.

(6) Dr. Jeffrey Stirman, PhD (Georgia Tech, 2012). Current position: Postdoctoral Fellow, UNC – Chapel Hill, NC.

(5) Dr. Alex Dajkovic (U. Kansas; postdoc, 2007-2009) Current position: Biology Research Project Leader, INRA, France.

(4) Dr. Yiider Tseng (Johns Hopkins U.; 1999-2005). Current position: Associate Professor, Chemical Engineering, Florida University, FL.

(3) Dr. Jingyuan “James” Xu (Johns Hopkins U.; Postdoc, 1997-1999). Current position: Physical Scientist, Plant Polymer Research, U.S. Department of Agriculture, IL.

(2) Dr. James L. Harden (UC-Santa Barbara; Postdoc, 1997-1998). Current position: Professor, Department of Physics, University of Ottawa, Canada.

(1) Dr. Thomas G. Mason (Princeton U.; Postdoc, 1997-1998). Current position: Professor, Department of Chemistry and Biochemistry, UCLA, CA.

IX. Presentations

Invited Talks at Universities (104) Cornell University, Chemical Engineering, March 2016 (103) Washington State University, School of Molecular Biosciences, Feb. 2016 (102) Cambridge University, Isaac Newton Institute, September 2015 (101) Rockefeller University, March 2015 (100) Princeton University, Biophysics Program, October 2014 (99) Virginia Tech, Department of Mechanical Engineering, March 2014 (98) University of Michigan, Department of Chemical Engineering, February 2014 (97) University of Arizona, Department of Physics, February 2014 (96) Johns Hopkins School of Medicine, Departments of Oncology and Pathology,

annual meeting, Longrifles, January 2014 (95) Johns Hopkins School of Medicine, Department of Cell Biology, September

2013. (94) Indiana University, Department of Chemistry, April 2013 (93) Carnegie Mellon University, Department of Biomedical Engineering, March 2013 (92) MIT, Koch Institute for Integrative Cancer Research, Oncology/PSOC, March

2013 (91) Rice University, Department of Bioengineering, February 2013 (90) University of Virginia, Department of Cell Biology, January 2013 (89) Syracuse University, Physics Department, December 2012 (88) Georges Washington University, Physics Department, October 25, 2012 (87) Cedars-Sinai Hospital, Department of Oncology, Los Angeles, CA, April 2012 (86) MIT, Department of Bioengineering, PSOC, March 2012 (85) Northwestern University, Physical Science-Oncology Center, Evanston, IL,

February 2012 (84) University of Southern California, Physical Science-Oncology Center, Los

Angeles, CA, January 2012 (83) Institut Curie, Paris, France, January 2012

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(82) California Institute of Technology, Caltech Bioengineering Lecture Series, Pasadena, CA, November 2011

(81) Johns Hopkins University School of Public Health, Program in Respiratory Biology & Lung Disease, October 2011.

(80) University of Basel, Department of Biochemistry, Switzerland, October 2011 (79) University of Kansas, Center for Nanotechnology Training Center, KA, October

2011 (78) Johns Hopkins University, Department of Pathology, Pathological Rounds,

Baltimore, MD, July 2011 (77) Pontifica Universidad Catolica de Chile, School of Biology, Santiago, Chile, July

2011 (76) Pontifica Universidad Catolica de Chile, School of Engineering, Santiago, Chile,

July 2011 (75) UC Santa Barbara, Kavli Institute for Theoretical Physics, “Soft Matter in

Biology: Experiments and Theory”, Santa Barbara, CA, May 2011 (74) The State University of New York (SUNY) at Buffalo, NY, April 2011 (73) Purdue University, Department of Chemical Engineering, Lafayette, IN,

December 2010 (72) University of Minnesota, Department of Biomedical Engineering, Minneapolis,

MN, November 2010 (71) University of North Carolina, Department of Physics, Chapel Hill, NC, October

2010 (70) Georgia Tech, Department of Chemical Engineering, Atlanta, GA, October 2010 (70) Vanderbilt University, Department of Chemical Engineering, Memphis, TN,

September 2010 (69) University of Southern California, USC Physical Sciences - Oncology Center, Los

Angeles, CA, June 2010 (68) University of Virginia, NanoStar Institute, Charlottesville, VA, May 2010 (67) Northwestern University, H Foundation and Robert Lurie Cancer Center,

Evanston, IL, April 2010 (66) Catholic University of America, Department of Physics, Washington DC,

February 2010 (65) Institut Curie, Paris, France, January 2010 (64) Illinois Institute of Technology, Department of Chemical Engineering, Chicago,

IL, March 2009 (63) University of Illinois at Urbana-Champaign, Department of Chemical

Engineering, Champaign, IL, February 2009 (62) Northwestern University, Department of Cell Biology, Medical School, Chicago,

IL, December 2008 (61) National University of Singapore, Singapore, November 2008 (60) Johns Hopkins School of Medicine, Department of Urology, Baltimore, MD,

October 2008 (59) Duke University, Department of Biomedical Engineering, Durham, NC, October

2008 (58) U.C. Berkeley, Department of Bioengineering, Berkeley, CA, September 2008

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(57) Warwick University, Department of Physics, Warwick, England, April 2008 (56) National University of Singapore, Department of Biological Sciences, Singapore,

February 2008 (55) Durham University, Department of Biological Sciences, Durham, England,

Keynote Talk, November 2007 (54) Cambridge University, Cavendish Laboratory, Cambridge, England, from

biophysics to medicine, Keynote Talk, June 2007 (53) University of Texas, Department of Chemical Engineering, IGERT seminar

series, Austin, TX, April 2007 (52) University of Connecticut, Health Center, Department of Cell Biology, Physics

and Biology seminar series, Farmington, CT, April 2007 (51) University of Connecticut, Health Center, Center for Computational Biology,

Farmington, CT, April 2007 (50) Washington University, Biomedical Engineering Department, St Louis, MO,

April 2007. (49) MIT, Department of Chemical Engineering, Cambridge, MA, October 2005 (48) University of Florida, Department of Chemical Engineering, Gainesville, FL,

October 2005 (47) Stanford University, Department of Chemical Engineering, Stanford, CA,

October 2005 (46) Ecole Federale Polytechnique de Lausanne (EPFL), Division of Life Sciences,

Lausanne, Switzerland, May 2005 (45) Johns Hopkins University, Department of Biology, symposium on the interface of

biology and engineering, Baltimore, MD, January 2005 (44) Johns Hopkins Medical School Department of Internal Medicine, Johns Hopkins

Medical School, Baltimore, MD, October 2004 (43) University of Pennsylvania, Institute of Medicine and Engineering, Philadelphia,

PA, March 2004 (42) The Scripps Research Institute, Department of Cell Biology, La Jolla, CA,

January 2004 (41) Notre Dame University, Department of Chemical and Biomolecular Engineering,

November 2003 (40) National Institutes of Health, Institute of Childs Health and Development,

Bethesda, MD, June 2003 (39) Johns Hopkins University, Department of Civil engineering, Baltimore, MD,

November 2002 (38) The Carnegie Institute of Washington, Department of Embryology, Baltimore,

MD, October 2002 (37) The Institute of Genomic Research, Gaithersburg, MD, January 2000 (36) Inc. R&D Labs, Columbia, MD, December 1999 (35) Columbia University, Biomedical Engineering Department, New York City, NY,

April 1999 (34) Stanford University, Chemical Engineering Department, Stanford, CA, April

1999

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(33) Columbia University, Department of Chemical Engineering, New York City, NY, February 1999

(32) University of Pennsylvania, Department of Physiology and Pennsylvania Muscle Institute, School of Medicine, Philadelphia, PA, January 1999

(31) National Institutes of Health, Department of Physical Biology, Bethesda, MD, December 1998

(29) Albert Einstein College of Medicine, Department of Physiology and Biophysics, Bronx, NY, November 1998

(28) National Institutes of Health, Structural Biology, Bethesda, MD, October 1998 (27) Rutgers University, Chemical Engineering Department, New Brunswick, NJ,

October 1998 (26) Johns Hopkins University, Department of Biology, Baltimore, MA, October 1998 (25) Texas A&M University, Department of Chemical Engineering, College Station,

TX, October 1998 (24) Johns Hopkins University, Department of Biological Chemistry, School of

Medicine, Baltimore, MD, May 1998 (23) University of California at San Diego, Bioengineering Department, La Jolla, CA,

April 1998 (22) University of Maryland at Baltimore County, Chemical Engineering Department,

Baltimore, MD, April 1998 (21) Vanderbilt University, Department of Biological Chemistry, School of Medicine,

Nashville, TN, April 1998 (20) National Institutes of Health, Biotechnology group, Bethesda, MD, March 1998 (19) University of Massachusetts, Polymer Science and Engineering, Amherst, MA,

March 1998 (18) City College of New York, Department of Chemical Engineering, New York

City, NY, February 1998 (17) Penn State University, Chemical Engineering Department, College Station, PA,

February 1998 (16) Institut Charles Sadron, Polymer Science and Engineering, Strasbourg, France,

December 1997 (15) Johns Hopkins University, Department of Mechanical Engineering, Baltimore,

MD, April 1997 (14) Merck Corporation, West Point Research Labs, PA, March 1997 (13) Rockefeller Institute, Physical Sciences, New York City, NY, January 1997 (12) University of North Carolina, Department of Chemistry, Rayleigh, NC,

September 1996 (11) NIST, Gaithersburg, MD, March 1996 (10) Institut Curie, Paris, France, December 1995 (9) ETH Zurich, Institute of Mechanics, Switzerland, December 1995 (8) Johns Hopkins University, Biophysics Department, Baltimore, MD, December

1995 (7) NIST, Gaithersburg, MD, March 1995 (6) 3M Corporate Research Laboratories, St. Paul, MN, February 1995 (5) ESPCI, Groupe de Physico-Chimie Théorique, Paris, France, April 1994

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(4) ESPCI, Groupe de Physico-Chimie Théorique, Paris, March 1994 (3) Johns Hopkins University, Department of Chemical Engineering, Baltimore, MD,

April 1993 (2) University of Southern California, Department of Chemical Engineering, Los

Angeles, CA, March 1993 (1) Yale University, Department of Chemical Engineering, New Haven, CT, March

1993

Invited Talks at Conferences (62) Aspen Center for Physics, March 2016 (61) ASCB meeting, San Diego, December 2015 (60) NCI PI Meeting, nanotechnology in cancer, November 2015 (59) MRS meeting, Boston, November 2015, (58) Cell-Matrix Mechanobiology: Current State and Future Directions, University of

Illinois at Urbana Champaign, October 2015. (57) Cell Mechanics, Morphogenetics and Pattern Formation: perspectives from

experimental and theoretical points of view, Isaac Newton Institute in Cambridge University, September 2015.

(56) ASME meeting, NEMB, Minneapolis, April 2015 (55) PSOC meeting, July 2014 (54) BMES meeting, Seattle, 2013 (53) ESIFB, Amsterdam, September 2013. (52) Gordon Conference on Motile and Contractile Systems, Colby College, August

2013. (51) Institute for Cancer Research and Treatment, Strada Provinciale, EMBO

conference, Italy, March 2013 (50) ASME, Nanoengineering for Medicine and Biology, February 2013. (50) ASCB annual meeting, Physics of Cancer, San Francisco, December 2012. (49) BioImaging, Porto, Portugal, September 2012 (48) IMEC annual conference, Brussels, Belgium, May 2012 (47) Experimental Biology annual meeting, San Diego, April 2012 (46) Tutorial on Physics of Cancer, APS annual meeting, February 2012 (45) NCI "Integrating the Physical Sciences Perspective to Open a New Frontier in

Oncology" Meeting, Bethesda, MD, February 2012 (44) NCI Physical Science Oncology Centers, Directors retreat, December 2011 (43) AIChE annual meeting, Plenary talk Section 15c/d, Minneapolis, MN, October

2011 (42) Physics of Cancer, Leipzig, Germany, October 13-15 (2011) (41) Banff International Research Station (BIRS), “Mathematical Biology of the Cell:

Cytoskeleton and Motility”, Banff, Canada, July 31 - August 5, 2011 (41) International Biophysics Conference, San Diego, July 2011 (40) Kavli Institute for Theoretical Physics, “Soft Matter in Biology: Experiments and

Theory”, UC Santa Barbara, CA, May 23-27, 2011 (39) Biophysical Society Workshop on Actin, the cytoskeleton, and the nucleus,

Singapore, November 2010

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(38) Physical Sciences in Oncology meeting, tutorial on particle tracking microrheology, New Harbor, MD, March 2010

(37) GEM4 short course, University of Texas, Austin, TX, January 2010 (36) American Society for Cell Biology, San Diego, CA, December 2009 (35) Biological and Soft Matter, Warwick, England, April 2009 (34) American Physical Society, Physics of cancer, March 2009 (33) Mid-Atlantic, Soft Condensed Matter Workshop, NIST, February 2009 (32) American Chemical Society, Philadelphia, August 2008. (31) American Physical Society, Responsive and adapatable polymeric materials,

Denver, CO, March 2007 (30) 13th Yonsei International Symposium, Keynote Talk, Seoul, Korea, November

2006 (29) Biomedical Engineering Society meeting, Cellular and Molecular Engineering -

Mechanobiology of the cell-Substrate Interface, Chicago, IL, October 2006 (28) Biomedical Engineering Society meeting, Cardiovascular Engineering -

Mechanobiology of the Cytoskeleton II, Chicago, IL, October 2006 (27) Biomedical Engineering Society meeting, Cellular and Molecular Engineering -

Mechanics of single molecules and small assemblies, Chicago, IL, October 2006 (26) 2nd Annual Meeting of the American Academy of Nanomedicine, cellular

nanomedicine, Washington DC, September 2006 (25) 5th World Congress of Biomechanics, Mechanobiology at micro- and nano-scale

levels, Munich, Germany, July 2006 (24) 5th World Congress of Biomechanics, Molecular Biomechanics: Biomechanics at

micro- and nanoscale Levels, Munich, Germany, July 2006 (23) Gordon Research Conferences, Biointerfaces, Les Diablerets, Switzerland,

October 2006 (22) AIChE meeting, keynote, Fluids, Cincinnati, OH, November 2005 (21) Society of Rheology meeting , symposium on rheology of biological systems,

Vancouver, Canada, October 2005 (20) Biomedical Engineering Society meeting, symposium on new frontiers in cancer

bioengineering, Baltimore, MD, October 2005 (19) ACS meeting, Symposium on new frontiers in ultrasensitive analysis:

nanobiotech, single molecule detection, and single cell analysis, Washington DC, September 2005

(18) 2005 Pacific-Rim Congress on Rheology, Shanghai, China, August 2005 (17) International Congress on Rheology, Seoul, Korea, August 2004 (16) Workshop on Physics of Biological Polymers, Institute of Physics, London,

England, August 4, 2003 (15) Workshop Cytomechanics Modules, Friday Harbor Labs, University of

Washington, July 10, 2003 (14) 3rd Chemical Engineering Conference for Collaborative Research in Eastern

Mediterranean, Nanoscience in Chemical Engineering, Thessaloniki, Greece, May 14-16, 2003

(13) American Society for Cell Biology, Mini-symposium on Actin in the Nucleus, December 13-17, 2002

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(12) Gordon Research Conference “Biophysical approach to Intermediate filament biology”, Roger Williams University, June 30-July 5, 2002

(11) AIChE annual meeting, Special session “Nanotools", Reno, NE, Nov. 4, 2001. (10) Society for Experimental Biology, annual meeting, Miami, FL, April 1, 2001 (9) Biomedical Engineering Society, BMES/AIChE/ACS joint meeting, Snowbird,

UT, August 1, 2001 (8) England Society of Microscopy, annual meeting, Woods Hole, MA, May 1 2001 (7) AIChE annual meeting, Special session on novel flows, Dallas, TX, November

1999 (absent, given by M. Pasquali) (6) Society of Rheology, Symposium in honor of Prof. Ferry, WN, October 1999 (5) American Society of Biomedical Engineering, Annual meeting, Cleveland, OH,

October 1998 (absent, given by S.C. Kuo) (4) European Research Conference, Biophysics of Cytoskeleton, Obernai, France,

April 1998 (3) Fine Particle Society, Annual meeting, Dallas, TX, March 1998 (2) American Physical Society, Annual meeting, Los Angeles, CA, March 1998 (1) Biophysical Society, symposium on biotechnology, Annual meeting, Kansas City,

MS, March 1998

Articles Reviewed for the Following Journals (last 2 years) Nature, Science, PNAS, Nature Cell Biology, Journal of Cell Biology, Journal of

Cell Science, Molecular Biology of the Cell, Journal of Molecular Biology, Journal of Biological Chemistry, Physical Review Letters, and Biophysical Journal, Physical Biology

Proposals Reviewed for the Following Agencies (last 4 years)

National Institutes of Health, National Science Foundation, Science Foundation Ireland, Science Foundation Netherlands, U.S.-Israel Binational Science Foundation, funding agencies in Norway and Sweden.