dehart mabsa talk - mabsa provided by
TRANSCRIPT
Synthetic BiologySynthetic Biology
A Biohacker’s Perspective
[Modified from Sprinzak and Elowitz, 2005]
[Modified from Gardner et al., 2000]
A B Positive feedback loopNegative feedback loop
[Modified from Benner and Sismour, 2005]
[Modified from Purnick and Weiss, 2009]
[Mukherji and van Oudenaarden, 2009]
[Modified from Rothemund, 2006]
[Modified from Andersen et al., 2009] [Modified from Hung et al., 2009]
[Modified from Qian and Winfree, 2011]
Engineered
Natural
[Modified from Benner and Sismour, 2005]
[syntheticgenomics.com]
[Gibson et al., 2010]
MOTIVATIONS OF A BIOHACKER“What I cannot build, I cannot understand.” – Richard Feynman
MOTIVATIONS OF A BIOHACKER
[http://2010.igem.org/Image:TU_Delft_ethics_arabidopsis.jpg][http://www.newscientist.com/gallery/microbe‐art/7] [http://www.wired.com/wiredscience/2008/11/amid‐doom‐synth/]
[http://adambluestein.com/2009/11/visible‐results‐2009‐igem‐roundup/][http://adambluestein.com/2009/11/visible results 2009 igem roundup/]
http://www.nytimes.com/imagepages/2010/12/19/JP‐AMATEUR2.html
http://www.nytimes.com/2010/12/19/fashion/19amateur.html?_r=1&ref=fashion
[http://xeobits.com/wp‐content/uploads/2010/12/steve‐jobs‐Steve‐wozniak.jpg]
[http://rt.com/news/biohacking‐harmless‐hobby‐or‐global‐threat/]
[http://magazine.ucla.edu/features/outlaw_biology/]
[http://diybio.org/safety/biosafety‐resources]
[http://www.fas.org/blog/bio/tag/synthetic‐biology/] [http://oba.od.nih.gov/biosecurity/pdf/NSABB%20SynBio%20DRAFT%20Report‐FINAL%20(2)_6‐7‐10.pdf]
[http://www.bioethics.gov/documents/synthetic‐biology/PCSBI‐Synthetic‐Biology‐Report‐12.16.10.pdf]
HOW TO ENGINEER BIOLOGYHOW TO ENGINEER BIOLOGY
LAYER DEFINITION
System Multiple devices connected together to realize a goalSystem Multiple devices connected together to realize a goal
Device Multiple parts with a higher level function
Part A finite sequence of nucleotides with a specific function
[http://ocw.mit.edu/courses/biological‐engineering/20‐020‐introduction‐to‐biological‐engineering‐design‐spring‐2009/lecture‐and‐studio‐notes/wk07/]
DNA Sequence of nucleotides
[http://partsregistry.org/Main_Page]
[http://www.neb.com/nebecomm/products/productE0546.asp]
[http://ginkgobioworks.com/works.html]
[Modified from Martin et al., 2009]
Input Output
[Canton et al., 2008]
[http://ginkgobioworks.com/works.html]
P
GeneR R P
Promoter
Repressor Protein R P
Absent Present
Present Absent
0 1
1 0
Inverter
[Canton et al., 2008]
Plasmid DNA
Bacterial Cells Mammalian Cells
Yeast Cells
[Purnick and Weiss, 2009]
THE IGEM COMPETITIONTHE IGEM COMPETITION
The challenge: Use (or create) standard biological parts to construct a novel biological t bl f l i l ld isystem capable of resolving a real‐world issue
The rules:1) Undergraduate or high school team members only2) All progress must be meticulously documented in an online wiki lab notebook3) All work must be correctly attributed to the original researcher(s)4) All parts must be constructed to Registry standards and all part DNA must be sent to
the Registry (after being sequence verified)the Registry (after being sequence‐verified)5) All participants must adhere to the safety guidelines in order to minimize any
potential risks from newly constructed parts (e.g. adenovirus gene transfer vectors)
[http://2011.igem.org/Requirements]
[Modified from http://parts.mit.edu/wiki/index.php/Schools_Participating_in_iGEM_2006]
[http://ung.igem.org/Results?year=2010]
[http://2011.igem.org/Safety]