December 21, 2017 FINAL REPORT Type of Award: Orthodontic Faculty Development Fellowship Award (OFDFA) - A126712 Principal Investigator: Andrew Jheon Title of Project: Alterations in the subgingival microbiome during orthodontic treatment Period of AAOF Support: 07-01-15 to 06-30-16 (1st NCE to 6-30-17; 2nd NCE to 12-31-17) Amount of Funding: $20 000 Summary/Abstract of Completed Project Results (250 word maximum): Introduction: To evaluate changes in the subgingival microbiome before and during fixed appliance

orthodontic treatment using 16S rRNA-based high-throughput sequencing. Methods: Sixteen patients (10

females and 6 males; ages 12y1m-33y3m) were included in this study. Subgingival microbial samples were

collected from 4 teeth of each subject at three different times before and during full-fixed appliance treatment;

two patients received clear aligner therapy (i.e., Invisalign). DNA was extracted from the samples, and 16S

rRNA-based, next generation sequencing (NGS) was performed catalog global profiles of subgingival

microbiome during orthodontic treatment. Results: The frequency of T forsythia, C rectus, and P nigrescens

significantly increased after placement of orthodontic appliances. For the other species, the frequency tended

to increase but no statistically significant differences were noted. The frequency of the change, representing

microorganisms not existing at t0 but newly developing at t1 and t2, was higher at the molars than at the

incisors. Although sample size was limited, there also appeared to be significant changes in patients receiving

clear aligner therapy. Conclusion: Orthodontic treatment, whether utilizing full fixed appliances or clear

aligners significantly alters subgingival microbial composition and consensus bacterial species that were

altered were identified. Our experiments lay a solid foundation for further analysis of subgingival microbiome

during orthodontic therapy.

Response to the following questions: 1. Were the original, specific aims of the proposal realized? The original aim was “to profile changes in microbial composition during fixed orthodontic treatment using 16S rRNA next generation sequencing (NGS). Yes, this original aim has been realized. Our study was the first time that the oral microbiome was globally profiled during orthodontic treatment using NGS. 2. Were the results published?

No not yet. However, the manuscript is in preparation for submission to AJODO (I have attached the current draft). In addition, we will use our data as preliminary results to apply for NIDCR R21 funding for an expanded study. a. If so, cite reference/s for publication/s including titles, dates, author or co-authors, journal, issue and page numbers. Isaac Chen, Jennifer Chung, Yanjiao Zhao, Andrew H. Jheon (in preparation) Alterations in the subgingival microbiome during orthodontic treatment. AJODO. b. Was AAOF support acknowledged? Yes. c. If not, are there plans to publish? Yes, please see above. 3. Have the results of this proposal been presented? No a. If so, list titles, author or co-authors of these presentation/s, year and locations: N/A b. Was AAOF support acknowledged? N/A c. If not, are there plans to do so? If not, why not? No because we did not budget for any travel to conferences. 4. To what extent have you used, or how do you intend to use, AAOF funding to further your career? I have applied for BRA funding for 2018 and hope to continue to apply for AAOF funding in the future. It is a valuable source of funding outside of the NIH for my research and laboratory. Accounting for Project; i.e., any leftover funds, etc. There are no leftover funds. I apologize that this project took much longer than anticipated. This was largely due to this being my first foray into human microbiome research. Thus, I did not properly account for timing of human research approval and data analysis. Thank you for your patience. Please return to AAOF via email attachment to aaofevp@aaortho.org

Alterations in the subgingival microbiome during orthodontic treatment

Isaac Chen,1 Jennifer Chung,2 Yanjiao Zhao,2 Andrew H. Jheon3*

1Division of Orthodontics, University of California San Francisco (UCSF), San

Francisco, CA USA 94143

2Jackson Laboratory for Genomic Medicine, 10 Discovery Dr, Farmington, CT USA

06032

3Divisions of Orthodontics and Craniofacial Anomalies, UCSF, San Francisco, CA USA

94143

*Corresponding Author:

Andrew H. Jheon PhD, DDS, Ortho (cert.) Assistant Professor UCSF School of Dentistry Department of Orofacial Sciences Division of Craniofacial Anomalies 513 Parnassus Ave HSW815 San Francisco, CA 94143, Box 0422 Tel: 415.502.2730; Fax: 415.476.9513 Email: andrew.jheon@ucsf.edu

Highlights

1) Our study is the first to globally profile bacteria using 16S rRNA-based, next

generation sequencing (NGS) to catalog changes in subgingival microbiota during

orthodontic treatment.

2) There are dramatic alterations in subgingival microbiome during full fixed appliance

orthodontic treatment.

3) Although there is little consensus in microbial profile in teeth between subjects and

within the same subject, there are several consensus bacterial species that are

significantly changed during orthodontic treatment.

4) Although subjects were limited, the use of clear aligners (e.g., Invisalign) also

showed significant changes in subgingival microbiome during treatment, perhaps

surprising since a company-touted advantage of clear aligners is their perceived neutral

effect on oral hygiene.

5) Our study builds a strong foundation to further analyze the role of subgingival

microbiome in orthodontic tooth movement utilizing full fixed appliance and clear aligner

therapies.

ABSTRACT

Introduction: To evaluate changes in the subgingival microbiome before and during

fixed appliance orthodontic treatment using 16S rRNA-based high-throughput

sequencing. Methods: Sixteen patients (10 females and 6 males; ages 12y1m-33y3m)

were included in this study. Subgingival microbial samples were collected from 4 teeth

of each subject at three different times before and during full-fixed appliance treatment;

two patients received clear aligner therapy (i.e., Invisalign). DNA was extracted from the

samples, and 16S rRNA-based, next generation sequencing (NGS) was performed

catalog global profiles of subgingival microbiome during orthodontic treatment. Results:

The frequency of T forsythia, C rectus, and P nigrescens significantly increased after

placement of orthodontic appliances. For the other species, the frequency tended to

increase but no statistically significant differences were noted. The frequency of the

change, representing microorganisms not existing at t0 but newly developing at t1 and

t2, was higher at the molars than at the incisors. Although sample size was limited,

there also appeared to be significant changes in patients receiving clear aligner therapy.

Conclusion: Orthodontic treatment, whether utilizing full fixed appliances or clear

aligners significantly alters subgingival microbial composition and consensus bacterial

species that were altered were identified. Our experiments lay a solid foundation for

further analysis of subgingival microbiome during orthodontic therapy.

Introduction

In 2007, the National Institutes of Health (NIH) funded the human microbiome project

(HMP) to map microbial makeup of healthy humans in an effort to better understand

correlation of the microbiome with human health [1]. It was discovered that nearly

everyone routinely carries pathogens and microorganisms known to cause illnesses.

However, in healthy individuals pathogens cause no disease and simply coexist with

their host and the rest of the microbiome [1]. Recently, Sender et al. [2] published a

revised estimate of bacteria to human cell ratio to be nearly 1:1, an estimated 38 trillion

bacteria to 30 trillion human cells. Every human body contains personalized

microbiomes that are essential to maintain health, but also capable of eliciting disease.

For example, there is a concentration of 1011 bacteria/ml in the gut that provide both

beneficial and harmful effects [2]. This gut flora enhances our immune system, helps

absorb vitamins, and utilizes the food consumed. Conversely, the gut flora is also

associated with obesity, intestinal and systemic inflammation, and even cancer and

autism [3].

Bacterial diversity in dental plaque and oral biofilm is estimated to include at least

800 different species, consisting of a wide variety of gram-positive and gram-negative

bacteria [4]. The number of species is expected to rise into the thousands with the

application of next-generation sequencing (NGS) techniques [5, 6]. Within the oral

cavity, there are three main types of surfaces for bacteria to colonize: the hard surfaces

of teeth (i.e., supra- and sub-gingival), the soft tissues of the oral mucosa, and saliva

[7]. Furthermore, the oral microbiome is extremely dynamic because of the oral cavity’s

continual exposure with the external environment [8]. Saliva has a bacterial

concentration of 109 bacteria/ml whereas dental plaque has an equivalent bacterial

concentration as the colon (i.e., 1011 bacteria/ml) [2]. Studies have shown the oral

cavity’s microbiome to be a key source in the etiology of many oral and systemic

diseases [9, 10].

Dental plaque is a dynamic, complex biofilm and a microbial ecosystem [11].

Bacterial diversity in dental plaque or oral biofilm is estimated to include at least 800

different species, consisting of a wide variety of gram-positive and gram-negative

bacteria [4, 12-15]. The number of species is expected to rise into the thousands with

the application of next-generation sequencing (NGS) [5, 6]. In addition, many species in

kingdom Monera, also known as archaebacteria, have been associated with oral

disease such as chronic periodontitis [16, 17]. On top of the staggering number of

bacteria and archaebacteria, dental plaque comprises an assortment of micro-niches,

metabolic functions, and a web of inter- and intra-species interactions that accumulates

through sequential and ordered colonization by different bacterial strains and species

[18]. Biofilms develop under a wide range of different conditions, environments, and

factors (e.g., pH, availability of oxygen and nutrients, and interbacterial co-adhesion),

and these differences all have been demonstrated to lead to compositional changes in

oral biofilm [18]. Oral biofilm profiling has been hampered because it is extremely

difficult to define normal microbiota because of individual variation [19]. However, dental

health has been associated with the absence [20, 21] or elevation [22-24] of certain

species.

The composition of subgingival microbiota can be influenced by several factors

including oral hygiene regimens, dental restoratives, and orthodontic appliances. The

etiology of gingivitis and periodontitis is microbial infection, resulting in an imbalance

between the host and subgingival microorganisms [17]. Fixed appliances can change

the subgingival microbial environment by increasing plaque accumulation and

deepening the gingival sulcus [25, 26]. Orthodontic appliances generally increased the

level of periodontopathogens in subgingival plaques [27-29], however, several studies

have reported no significant difference [30] or a decrease in the level of

periodontopathogens decreased during orthodontic treatment due to metal corrosion,

which imposed toxic effects on the microorganism [31]. Thus, previous studies

regarding changes in periodontopathogens during orthodontic treatment have been

inconsistent.

All previous studies involving orthodontic full fixed appliances have used

microbial analysis methods such as candidate-based PCR or bacterial culture methods

[30], but these techniques suffer from selection biases or difficulty in growing cultures.

16S rRNA combined with next generation sequencing (NGS) has been used previously

to profile the microbiome in health and disease including periodontal diseases [32-36],

but never during orthodontic treatment. 16S rRNA/NGS catalogs all human and

microbial DNA in each of the samples, which is then sorted to identify specific genetic

signals found only in bacteria — such as the variable genes of bacterial ribosomal RNA

called 16S rRNA that can identify the presence of different microbial species [1]. The

purpose of this study is, for the first time, to track global changes in subgingival bacterial

composition before and after the placement of patients undergoing traditional full fixed

appliance orthodontic treatment.

Materials and Methods

Subjects

Sixteen subjects (10 Females and 6 Males; ages 16.2-17.5) were selected among

patients who arrived for orthodontic treatment at the Department of Orthodontics,

University of California San Francisco. Six patients failed to complete the entire study.

The study design was approved by the Ethics Committee (IRB 15-17868). Subjects

were enrolled according to the following criteria: (1) no known systemic disease; (2) no

use of antimicrobial, antifungal or anti-inflammatory drugs within 3 months before the

baseline examination; (3) no craniofacial anomalies; (4) not-pregnant; and (5) would be

undergoing treatment with fixed orthodontic appliances in the mandibular arch. Five

subjects (4 female and 1 male; Age ??) not undergoing orthodontic treatment were

selected as a control group.

Orthodontic bonding

All subjects received oral hygiene instructions by the same clinician in the form of both

verbal instructions and an instructional video by Dolphin Aquarium (Patterson

Technology). Subjects were instructed to continue their routine oral hygiene regiment.

All subjects received orthodontic therapy with fixed buccal appliances. For orthodontic

treatment, metal brackets (3M Unitek, GAC, Opal or GAC innovation) were bonded

directly with composite resin (find brand of composite) onto incisors and premolars.

Bands (Unitek and GAC) were cemented with polyacid-modified composite resin (Ultra

Band-Lok) onto molars. The arch wires were tied using o-chains (find brand of o-chains)

and in one case self-ligated.

Clear aligner therapy

Two patients received clear aligner trays (Invisalign) and treatment was administered

following instruction from Invisalign.

Collection of subgingival microbiota

Subgingival crevicular fluid samples were collected from the straight buccal gingival

crevice of the lower right first molar (30B), lower right central incisor (25B), lower left

first premolar (21B) and the straight lingual of lower left first premolar (21L). Samples

were collected at three different time points: before appliance placement (t0), 6 weeks

(t1) and 12 weeks (t2) into treatment. The sampling sites were isolated using full mouth

isolators (Dentsply, GAC) and the tongue held back with a mouth mirror when sampling

tooth 21L. Two sterile paper points size 30 (Dentsply, GAC) were inserted into the

gingival crevice and were left in situ for 60 s. These paper points were transferred

immediately into Eppendorf tubes containing 250 mL of 0.9% saline solution (Baxter,

Grainger) and frozen at -80 degrees F.

Periodontal Assessment

Immediately after sampling the sites, the probing depths (PD), GM-CEJ measurement,

clinical attachment loss (CAL), and bleeding on probing (BOP) were measured and

recorded in Axium periodontal charting system for all three time points. A sterilized

periodontal probe (find brand) was used to measurement PD and GM-CEJ. BOP was

observed and classified as present or not present after the periodontal measurements.

All sampling and measurements were performed by the same clinician. Once all the

samples were collected, they were sent to Jackson Laboratory in Bar Harbor, ME for

16S rRNA gene sequencing and analysis.

16S rRNA gene sequencing: Metagenomic DNA from the sampled paper points was

extracted using a PowerSoil ® DNA Isolation Kit. 16S rRNA V1-3 regions from the

metagenomic DNA were amplified using primers 27F and 534R (27F:5'-

AGAGTTTGATCCTGGCTCAG-3' and 534R: 5'- ATTACCGCGGCTGCTGG-3').

Sequencing reads were processed by removing the sequences with low quality

(average qual <35) and ambiguous nucleotides (N’s); chimeric amplicons were removed

using UChime software. OTU was generated from the processed reads using an

automated pipeline. Each OTU was classified from phylum to genus level using the

most updated RDP classifier and training set. A taxonomic abundance table was

generated with each row as bacterial taxonomic classification, each column as sample

ID, and each field with taxonomic abundance. The abundance of a given taxon in a

sample was presented as relative abundance (the read counts from a given taxon

divided by total reads in the sample).

Statistical Analysis

Each sample was subsampled to the lowest number of read counts among samples in

the dataset and rarefied to 5000 read counts. The abundance of a taxon in a sample

was indicated as the relative abundance, which was calculated by dividing the number

of reads for a taxon by the total read counts of the sample. Alpha diversity indices

including richness and Shannon diversity were calculated as previously described. To

examine difference between control and bracket samples, we performed non-metric

multidimensial scaling (NMDS) plots with Bray-curtis distance. Microbiome stability in

both groups was determined by calculating the Bray-curtis dissimilarity from the same

samples across different time points.

Results (in preparation)

We obtained a mean of 23,755 sequences per sample and aggregated to a total of 402

operational taxonomic units (OTUs). In our braces cohort, we observed that there was a

significant increase in microbial richness between samples obtained before braces

intervention and six weeks after intervention among three of the four sites. Richness

stayed at a similarly high microbial richness by 12 weeks. This increase in richness was

accompanied by the decrease in Streptococcus, the most abundant genera found

across all samples. Similar trends were observed when looking at diversity among

samples by Bray Curtis. Samples taken before bracket treatment clustered more tightly

and differently compared to the two time points after, which clustered similarly among

each other. In addition, over 30% of our bracket cohort developed periodontitis

compared to 15% of our controls by six weeks of intervention. Overall samples that

were associated with periodontitis had a lower relative abundance of Streptococcus. We

also observed that there were fewer OTUs shared among the four sites at t0 relative to

t1 and t2, suggesting that the whole oral microbiome becomes more uniformed with

bracket treatment. Interestingly, the unique OTUs to each site over time did not remain

the same, suggesting the uniqueness of each tooth’s bacterial profile may be transient.

Discussion (in preparation)

One of the major problems associated with phase II, fixed appliance treatment is

its long duration, on average of 29 months [37]. There is empirical, clinical evidence that

increased bacterial plaque may decrease the rate of orthodontic tooth movement and

overall treatment times. In addition, the microbiome has recently been shown to

possess critical, much larger roles in health and disease, most notably with studies on

the effects of the gut microbiome on obesity [38], intestinal cancer [39], diabetes [40],

and autism [3]. Finally, the biological and mechanical changes induced by orthodontic

treatment would likely lead to changes in subgingival dental plaque. Thus, the

contribution of the microbiome, and specifically subgingival plaque, during orthodontic

therapy is an exciting possibility that has yet to be tested. Ultimately, manipulation of the

microbiome may be a therapeutic option to accelerate orthodontic tooth movement and

shorten treatment times.

We observed significant microbial changes in the oral microbiome of patients

with bracket intervention and the composition of these communities may contribute to

periodontitis.

Acknowledgment

We would like to thank the American Association of Orthodontics Foundation for

supporting this work through the Orthodontic Faculty Development Fellowship Award

(OFDFA) to A.H.J..

Figure Legends

Figure 1. Subgingival microbial profiles pooled for control (no treatment), full

fixed appliance (bracket), and clear aligner (invisalign) therapy. For all groups,

OTU_1.streptococcus is the most common bacterial genus, followed by OTU_3.

Streptococcus and OTU_2. Veillonella. In the bracket group, tooth 30B showed an

overall decrease in OTU_1. Streptococcus, whereas 25B and 21B showed decreases at

t1 followed by increases at t2 although not to the same levels at t0. 21L profile remained

relatively the same at all three time points. In the control group, the levels of OTU_1.

Streptococcus had similar abundance at all three time points for 30B and 25B, an

overall decrease in 21B but an increase in 21L. The bracket group showed an overall

decrease in OTU_3. Streptococcus from t0 to t2, whereas the control group showed an

overall increase in OTU_3. Streptococcus, except for tooth 30B. OTU_2. Veillonella

increased for bracket tooth 25B and 21L but decreased in 30B and 21B. In the control

group, OTU_2. Veillonella increased in tooth 25B, decreased in 30B and 21B, and was

barely present in 21L. The amount of “other” bacteria was higher on the bracket group

than in the control group.

Figure. 2. Bray-Curtis, Richness, and Shannan diversity analyses. (A) The Bray-

Curtis analysis provides a measure of community composition differences between

samples based on OTU counts regardless of taxonomic assignment. Ordinations based

on this metric demonstrated a clear separation between control, bracket, and Invisalign

groups for tooth 30B. 25B and 21L were slightly more similar and 21B showed

significant separation between t0-t1 but were more similar at t1-t2. (B) The bracket

group demonstrated a significant increase in microbiome richness from t0 to t2,

whereas the control group only showed slight increases in microbiome richness from t0

to t2. (C) The Shannon diversity index (H) is commonly used to characterize species

diversity in a community. At t0 both bracket and control groups started with similar

diversity indexes. The bracket group showed an increase in diversity for tooth 30B and

21L at t1 and t2, but tooth 25B and 21B showed increases at t1 followed by decreases

at t3. In the control group, tooth 30B and 21B showed minimal changes in diversity, 25B

showed a decrease in diversity at t1 and an increase at t2, whereas tooth 21L showed a

decrease in diversity at t2.

Figure 3. Non-metric Multidimensial Scaling (NMDS) ordination analysis. To

evaluate the relationship between bacterial community composition and various

environmental factors, we performed NMDS analysis to represent pairwise Bray-Curtis

similarities between bacterial communities.

Figure 4. Global subgingival microbial profiles of individual subjects and teeth.

Figure 5. Venn diagram showing shared OTU or consensus bacterial species in

all teeth and time.

Figure 6. Venn diagram showing shared OTU or consensus bacterial species in

all teeth at the three time points.

Supplemental Figure 1. Top 10 up- and down-regulated bacterial species for each

tooth and subject at t0-t1 and t1-t2. Subjects are listed as A-O.

REFERENCES

1. Anon. NIH Human Microbiome Project defines normal bacterial makeup of the body. 2015.

2. Sender, R., S. Fuchs, and R. Milo, Revised Estimates for the Number of Human and Bacteria Cells in the Body. PLoS Biol, 2016. 14(8): p. e1002533.

3. Mulle, J.G., W.G. Sharp, and J.F. Cubells, The gut microbiome: a new frontier in autism research. Curr Psychiatry Rep, 2013. 15(2): p. 337.

4. Becker, M.R., et al., Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol, 2002. 40(3): p. 1001-9.

5. Dethlefsen, L., M. McFall-Ngai, and D.A. Relman, An ecological and evolutionary perspective on human-microbe mutualism and disease. Nature, 2007. 449(7164): p. 811-8.

6. Keijser, B.J., et al., Pyrosequencing analysis of the oral microflora of healthy adults. J Dent Res, 2008. 87(11): p. 1016-20.

7. Zaura, E., et al., Defining the healthy "core microbiome" of oral microbial communities. BMC Microbiol, 2009. 9: p. 259.

8. Parahitiyawa, N.B., et al., Exploring the oral bacterial flora: current status and future directions. Oral Dis, 2010. 16(2): p. 136-45.

9. Garcia, R.I., M.M. Henshaw, and E.A. Krall, Relationship between periodontal disease and systemic health. Periodontol 2000, 2001. 25: p. 21-36.

10. Scannapieco, F.A., G.D. Papandonatos, and R.G. Dunford, Associations between oral conditions and respiratory disease in a national sample survey population. Ann Periodontol, 1998. 3(1): p. 251-6.

11. Marsh, P.D., The significance of maintaining the stability of the natural microflora of the mouth. Br Dent J, 1991. 171: p. 174-177.

12. Aas, J.A., et al., Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol, 2008. 46(4): p. 1407-17.

13. Paster, B.J., et al., Bacterial diversity in human subgingival plaque. J Bacteriol, 2001. 183(12): p. 3770-83.

14. Paster, B.J., et al., The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000, 2006. 42: p. 80-7.

15. Preza, D., et al., Bacterial profiles of root caries in elderly patients. J Clin Microbiol, 2008. 46(6): p. 2015-21.

16. Armitage, G.C., Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000, 2010. 53: p. 70-88.

17. Perez-Chaparro, P.J., et al., Newly identified pathogens associated with periodontitis: a systematic review. J Dent Res, 2014. 93(9): p. 846-58.

18. Filoche, S., L. Wong, and C.H. Sissons, Oral biofilms: emerging concepts in microbial ecology. J Dent Res, 2010. 89(1): p. 8-18.

19. Aas, J.A., et al., Defining the normal bacterial flora of the oral cavity. J Clin Microbiol, 2005. 43(11): p. 5721-32.

20. Eriksen, H.M. and V. Dimitrov, Ecology of oral health: a complexity perspective. Eur J Oral Sci, 2003. 111(4): p. 285-90.

21. Ledder, R.G., et al., Molecular analysis of the subgingival microbiota in health and disease. Appl Environ Microbiol, 2007. 73(2): p. 516-23.

22. Griffen, A.L., et al., Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing. ISME J, 2012. 6(6): p. 1176-85.

23. Kumar, P.S., et al., New bacterial species associated with chronic periodontitis. J Dent Res, 2003. 82(5): p. 338-44.

24. Kumar, P.S., et al., Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing. J Clin Microbiol, 2006. 44(10): p. 3665-73.

25. Kim, S.H., et al., Microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. Angle Orthod, 2012. 82(2): p. 254-60.

26. Naranjo, A.A., et al., Changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement. Am J Orthod Dentofacial Orthop, 2006. 130(3): p. 275 e17-22.

27. Lee, S.M., et al., Prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in Korean orthodontic patients. J Microbiol, 2005. 43(3): p. 260-5.

28. Rego, R.O., et al., Clinical and microbiological studies of children and adolescents receiving orthodontic treatment. Am J Dent, 2010. 23(6): p. 317-23.

29. Petti, S., E. Barbato, and A. Simonetti D'Arca, Effect of orthodontic therapy with fixed and removable appliances on oral microbiota: a six-month longitudinal study. New Microbiol, 1997. 20(1): p. 55-62.

30. Guo, R., et al., The microbial changes in subgingival plaques of orthodontic patients: a systematic review and meta-analysis of clinical trials. BMC Oral Health, 2017. 17(1): p. 90.

31. Speer, C., et al., Investigations on the influencing of the subgingival microflora in chronic periodontitis. A study in adult patients during fixed appliance therapy. J Orofac Orthop, 2004. 65(1): p. 34-47.

32. Bik, E.M., et al., Bacterial diversity in the oral cavity of 10 healthy individuals. ISME J, 2010. 4(8): p. 962-74.

33. Ge, X., et al., Oral microbiome of deep and shallow dental pockets in chronic periodontitis. PLoS One, 2013. 8(6): p. e65520.

34. Laksmana, T., et al., Metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study. Open Dent J, 2012. 6: p. 255-61.

35. Junemann, S., et al., Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing. PLoS One, 2012. 7(8): p. e41606.

36. Casarin, R.C., et al., Subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis. J Periodontal Res, 2013. 48(1): p. 30-6.

37. Vu, C.Q., et al., Treatment complexity index for assessing the relationship of treatment duration and outcomes in a graduate orthodontics clinic. Am J Orthod Dentofacial Orthop, 2008. 133(1): p. 9 e1-13.

38. Ridaura, V.K., et al., Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science, 2013. 341(6150): p. 1241214.

39. Schulz, M.D., et al., High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity. Nature, 2014.

40. Suez, J., et al., Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature, 2014.

30B 25B 21B 21L

t0 t1 t2 t0 t1 t2 t0 t1 t2 t0 t1 t2

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OTU_27.Prevotella

OTU_18.Leptotrichia

OTU_28.Streptococcus

OTU_5.Kingella

OTU_61.Capnocytophaga

OTU_36.Leptotrichia

OTU_11.Neisseria

OTU_67.Streptococcus

OTU_9.Neisseria

OTU_26.Capnocytophaga

OTU_19.Saccharibacteria_genera_incertae_sedis

OTU_29.Leptotrichia

OTU_55.Prevotella

OTU_12.Streptococcus

OTU_20.Granulicatella

OTU_25.Neisseria

OTU_210.Fusobacterium

OTU_10.Fusobacterium

OTU_33.Porphyromonas

OTU_350.Streptococcus

OTU_37.unclassified_Prevotellaceae

OTU_17.Granulicatella

OTU_8.Rothia

OTU_389.Streptococcus

OTU_14.Streptococcus

OTU_15.Gemella

OTU_294.Streptococcus

OTU_7.Neisseria

OTU_13.Fusobacterium

OTU_4.unclassified_Burkholderiales

OTU_2.Veillonella

OTU_3.Streptococcus

Others

OTU_1.Streptococcus

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Figure 2

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t0 t1 t2

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Figure 3

AB

CD

EF

GH

OI

JK

LM

NP

t0t1

t2

0 25 50 75

1000 25 50 75

1000 25 50 75

1000 25 50 75

100

Relative abundance (%)

OTU_Organism

01_OTU

_8 : Rothia aeria

02_OTU

_55 : Prevotella sp.

03_OTU

_17 : Granulicatella adiacens

04_OTU

_19 : Candidatus S

accharibacteria

05_OTU

_389 : Streptococcus m

itis

06_OTU

_10 : Fusobacterium nucleatum

07_OTU

_14 : Streptococcus gordonii

08_OTU

_15 : Gam

ella sp.

09_OTU

_7 : Neisseria m

ucosa

10_OTU

_294 : Streptococcus m

itis

11_OTU

_4 : Lautropia mirabilis

12_OTU

_13 : Fusobacterium nucleatum

13_OTU

_2 : Veillonella parvula

14_OTU

_3 : Streptococcus sanguinis

15_OTU

_1 : Streptococcus sp.

t0t1

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LIGN

30B25B21B21L

Other

Figure 4

30B

25B 21B

21L

10

13 4

3

5

19

2

6

4

4

24

68

7

265

BRACKET only: Shared otu in among all teeth among all timepoints

Figure 5

30B25B 21B

21L

14

42 17

126

22

2

9

19

4

19

1010

16

130

BRACKET only

t1

30B25B 21B

21L

19

19 9

513

7

7

8

9

5

11

159

10

206

t2

30B25B 21B

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9

12 6

74

8

6

4

6

2

21

1311

10

201

t3

Figure 6

SUPPLEMENTAL TABLE 1SubjectTooth1:TP2-TP1(30B:t1-t0)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Veillonelladispar16.8259831 Veillonelladispar40.853791 Streptococcussanguinis17.705844 Streptococcuspneumoniae/mitis/pseudopneumoniae82.9024549 Streptococcuspneumoniae/mitis/pseudopneumoniae74.9945688 Neisseriamucosa/macacae13.143602 Streptococcussanguinis12.3940908 Streptococcuspneumoniae/mitis/pseudopneumoniae63.7844884 Veillonelladispar6.49576363 Streptococcuscristatus7.82098631 Streptococcussanguinis30.5018466 Streptococcuspneumoniae/mitis/pseudopneumoniae13.6106887 Streptococcuspneumoniae/mitis/pseudopneumoniae8.61394743 Streptococcuspneumoniae/mitis/pseudopneumoniae81.0341082 Alloprevotellarava12.69824032 Neisseriamucosa/macacae14.8164241 Streptococcussanguinis6.44145123 Veillonelladispar7.12578753 Streptococcussanguinis4.00825549 Streptococcusdentisani4.71431675 Streptococcuspneumoniae/mitis/pseudopneumoniae9.14620899 Streptococcusvestibularis10.7429937 Gemellahaemolysans7.41907452 Neisseriamucosa/macacae5.45296546 Gemellahaemolysans2.31370845 Rothiadentocariosa3.45426896 Sphingomonasphyllosphaerae/Erythrobacterflavus3.51944384 Porphyromonaspasteri3.18270693 Gemellahaemolysans13.2739518 Veillonelladispar6.104714323 Porphyromonaspasteri12.0464914 Streptococcusgordonii5.76797741 Streptococcusvestibularis4.14946774 Streptococcuscristatus1.90093417 Gemellahaemolysans2.58527048 Streptococcussanguinis8.49446013 Rothiamucilaginosa2.59613296 Veillonelladispar3.66065609 Streptococcusvestibularis4.36671736 Capnocytophagaleadbetteri0.80382359 Streptococcuscristatus1.69454703 Gemellahaemolysans1.74885944 Gemellahaemolysans1.50988486 Streptococcusdentisani1.06452314 Veillonellaratti5.148815994 Fusobacteriumnucleatum4.71431675 Streptococcusdentisani5.26830328 Streptococcusgordonii3.01976972 Streptococcusdentisani1.53160982 Streptococcusgordonii1.05366066 Prevotellaloescheii4.1168803 Haemophilusparainfluenzae2.11818379 Streptococcusvestibularis1.95524658 Gemellahaemolysans3.56289376 Cardiobacteriumhominis0.61916142 Actinomycesoris1.41212253 Porphyromonascatoniae1.70540952 Porphyromonaspasteri1.07538562 Cutibacteriumacnes0.6843363 Porphyromonaspasteri4.583966985 Streptococcuspneumoniae/mitis/pseudopneumoniae4.49706713 Actinomycesviscosus2.35715837 Granulicatellaadiacens2.25939605 Gemellahaemolysans0.69519878 Streptococcusinfantis0.33673691 Lautropiamirabilis2.33543341 Streptococcusparasanguinis1.89007169 Rothiadentocariosa1.83575929 Streptococcusdentisani2.32457093 Fusobacteriumnucleatum0.46708668 Neisseriamucosa/macacae1.10797306 Neisseriaperflava1.55333478 Abiotrophiadefectiva0.35846187 Alloprevotellarava0.47794916 Porphyromonaspasteri2.813382586 Streptococcussanguinis4.30154247 Granulicatellaadiacens2.1724962 Novosphingobiumcolocasiae/soli/panipatense1.65109711 Actinomycesviscosus0.4562242 Haemophilusparainfluenzae0.18466218 Leptotrichiahofstadii2.11818379 Rothiadentocariosa1.65109711 Streptococcusdentisani0.92331088 Rothiamucilaginosa0.99934825 Capnocytophagagingivalis0.36932435 Streptococcusvestibularis0.97762329 Porphyromonaspasteri1.5424723 Rothiamucilaginosa0.24983706 Streptococcusgordonii0.4562242 Granulicatellaelegans2.780795137 Streptococcusdentisani2.6830328 Caenispirillumdeserti1.84662177 Streptococcusparasanguinis1.29263524 Neisseriamucosa/macacae0.39104932 Porphyromonaspasteri0.07603737 Fusobacteriumnucleatum1.69454703 Haemophilusparaphrohaemolyticus1.61850967 Granulicatellaadiacens0.90158592 Streptococcussanguinis0.90158592 Granulicatellaelegans0.34759939 Abiotrophiadefectiva0.77123615 Campylobactergracilis01.46643493 Alloprevotellarava0.08689985 Veillonelladispar0.33673691 Neisseriamucosa/macacae2.62872048 Abiotrophiadefectiva2.33543341 Actinomycesoris1.12969802 Lautropiamirabilis1.28177276 Actinomycesoris0.38018683 Neisseriaperflava0.06517489 Porphyromonascatoniae1.32522268 Streptococcusparasanguinis1.56419726 Streptococcusgordonii0.89072344 Chryseobacteriumcarnis0.72778623 Bergeyellazoohelcum0.33673691 Lautropiamirabilis0.58657397 Leptotrichiashahii0.84727352 Chryseobacteriumcarnis0.0543124 Streptococcussanguinis0.27156202 Porphyromonaspasteri2.444058229 Prevotellamelaninogenica1.65109711 Lautropiamirabilis0.76037367 Chryseobacteriumhaifense1.22746035 Streptococcusinfantis0.33673691 Chryseobacteriumcarnis0.06517489 Streptococcusdentisani0.93417337 Gemellahaemolysans0.78209863 Streptococcusinfantis0.23897458 Streptococcusgordonii0.51053661 Tessaracoccusdefluvii0.30414947 Caenispirillumdeserti0.56484901 Streptococcusinfantis0.80382359 Campylobactergracilis00.04344992 Veillonellaratti0.26069954 Gemellahaemolysans2.3028459710 Granulicatellaadiacens1.61850967 Kingellaoralis0.47794916 Streptococcusparasanguinis1.17314795 Granulicatellaadiacens0.2281121 Granulicatellaelegans0.0543124 Oribacteriumasaccharolyticum0.54312405 Streptococcusinfantis0.70606126 Haemophilusparainfluenzae0.19552466 Streptococcusinfantis0.46708668 Leptotrichiahongkongensis0.29328699 Gemellahaemolysans0.53226157 Gemellamorbillorum0.73864871 Neisseriaoralis0.03258744 Campylobactergracilis00.19552466 Neisseriacinerea2.0204214611 Fusobacteriumnucleatum-16.98892 Streptococcuspneumoniae/mitis/pseudopneumoniae-59.330871 Neisseriabacilliformis-10.417119 Leptotrichiawadei-23.680209 Neisseriamucosa/macacae-16.369759 Neisseriaelongata-18.998479 Veillonelladispar-17.434282 Lautropiamirabilis-13.969151 Streptococcuspneumoniae/mitis/pseudopneumoniae-22.63741 Streptococcuspneumoniae/mitis/pseudopneumoniae-3.3782316 Veillonelladispar-18.487943 Veillonelladispar-17.640669 Streptococcussanguinis-5.2465783 Fusobacteriumnucleatum-51.48816 Streptococcuspneumoniae/mitis/pseudopneumoniae-46.69780612 Prevotellaoris-10.677819 Gemellahaemolysans-2.7156202 Leptotrichiahongkongensis-7.0932001 Corynebacteriummatruchotii-19.117967 Prevotellaoris-9.0701716 Capnocytophagasputigena-6.6478384 Streptococcuspneumoniae/mitis/pseudopneumoniae-5.8222898 Prevotellaoris-13.382577 Neisseriaperflava-2.2268086 Neisseriaperflava-2.259396 Streptococcuspneumoniae/mitis/pseudopneumoniae-11.38388 Neisseriaoralis-4.3232674 Lautropiamirabilis-2.3897458 Capnocytophagaochracea-30.469259 Streptococcussanguinis-1.010210713 Corynebacteriummatruchotii-4.7143167 Rothiadentocariosa-2.4657832 Streptococcusintermedius-5.9091897 Selenomonasnoxia-9.0918966 Fusobacteriumnucleatum-7.2018249 Veillonelladispar-4.9098414 Streptococcusgordonii-3.0414947 Fusobacteriumnucleatum-11.351293 Fusobacteriumnucleatum-1.0427982 Streptococcussanguinis-1.8792092 Campylobactergracilis0-4.6056919 Streptococcusgordonii-4.1929177 Neisseriamucosa/macacae-2.1616337 Eikenellacorrodens-4.5731045 Lautropiamirabilis-0.423636814 Selenomonassputigena-3.9539431 Neisseriamucosa/macacae-0.9450358 Capnocytophagasputigena-5.0401912 Corynebacteriummatruchotii-5.2465783 Streptococcusintermedius-6.6804258 Neisseriaoralis-3.5085814 Neisseriamucosa/macacae-2.4114708 Anaeroglobusgeminatus-10.384532 Porphyromonaspasteri-0.4670867 Streptococcusinfantis-1.5967847 Granulicatellaadiacens-4.1168803 Streptococcussanguinis-3.3782316 Streptococcusinfantis-1.0645231 Aggregatibacteractinomycetemcomitans-2.2268086 Neisseriaperflava-0.260699515 Actinomycesdentalis-3.3130567 Streptococcusinfantis-0.4996741 Fusobacteriumnucleatum-4.9967413 Leptotrichiahofstadii-3.3782316 Capnocytophagaleadbetteri-4.6708668 Granulicatellaadiacens-2.3788833 Prevotellahisticola-1.7271345 Dialisterinvisus-9.9500326 Abiotrophiadefectiva-0.3258744 Neisseriamucosa/macacae-1.4881599 Streptococcusdentisani-2.2268086 Streptococcusdentisani-1.44471 Streptococcuscristatus-0.6408864 Parvimonasmicra-1.9009342 Neisseriaoralis-0.152074716 Atopobiumparvulum-3.1827069 Eggerthiacatenaformis-0.3475994 Campylobactergracilis0-3.3673691 Lachnoanaerobaculumsaburreum-3.3021942 Veillonelladispar-4.5731045 Capnocytophagagingivalis-1.4555725 Granulicatellaadiacens-1.0862481 Solobacteriummoorei-4.019118 Caenispirillumdeserti7-0.2824245 Neisseriacinerea-1.2600478 Corynebacteriummatruchotii-0.3150119 Abiotrophiadefectiva-1.3252227 Haemophilusparainfluenzae-0.6191614 Johnsonellaignava-1.8248968 Fusobacteriumnucleatum-0.119487317 Oribacteriumasaccharolyticum-2.9111449 Corynebacteriummatruchotii-0.2281121 Campylobactergracilis-2.84597 Campylobactergracilis-3.0849446 Streptococcusanginosus-3.8779057 Granulicatellaelegans-0.6082989 Leptotrichiawadei-0.8689985 Atopobiumrimae-2.84597 Streptococcusintermedius-0.1737997 Streptococcusgordonii-0.5213991 Gemellamorbillorum-0.3041495 Granulicatellaadiacens-1.1405605 Capnocytophagasputigena-0.5322616 [Eubacterium]yurii-1.6510971 Prevotellaintermedia-0.119487318 Prevotellaloescheii-2.2050836 Haemophilusparainfluenzae-0.1520747 Fusobacteriumnucleatum-2.7047578 Actinomycesdentalis-3.0306322 Actinomycesodontolyticus-3.117532 Leptotrichiashahii-0.5757115 Neisseriabacilliformis-0.6951988 Prevotellaloescheii-2.4657832 Lautropiamirabilis-0.1412123 Streptococcusvestibularis-0.3367369 Porphyromonaspasteri-0.293287 Lautropiamirabilis-1.1405605 Rothiadentocariosa-0.271562 Campylobactershowae-1.2926352 Haemophilusparainfluenzae-0.108624819 Actinomycesnaeslundii-2.0747339 Streptococcusparasanguinis-0.1520747 Kingellaoralis -2.3680209 Prevotellaoulorum-3.0089072 Capnocytophagasputigena-2.6613078 Rothiadentocariosa-0.5213991 Veillonellaatypica-0.6517489 Prevotellahisticola-1.2709103 Prevotellamelaninogenica-0.1412123 Rothiadentocariosa-0.3367369 Actinobaculumschaalii3-0.271562 Streptococcusintermedius-0.7495112 Granulicatellaadiacens-0.2498371 Treponemasocranskii-0.9341734 Atopobiumrimae-0.076037420 Selenomonasnoxia-2.0747339 Rothiamucilaginosa-0.1303498 Corynebacteriummatruchotii-2.1073213 Veillonelladispar-2.6721703 Streptococcussanguinis-2.6613078 Qipengyuaniasediminis/Altererythrobactermarinus-0.4019118 Megasphaeramicronuciformis-0.586574 Campylobactergracilis0-1.1731479 Haemophilusparaphrohaemolyticus-0.1086248 Granulicatellaadiacens-0.3041495 Kingellaoralis -0.1846622 Rothiadentocariosa-0.7495112 Granulicatellaelegans-0.2389746 Brachymonasdenitrificans-0.5974365 Prevotellaveroralis-0.0760374Tooth1:TP3-TP2(30B:t2-t1)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Prevotellaoris7.34303715 Streptococcuspneumoniae/mitis/pseudopneumoniae61.9487291 Leptotrichiawadei19.400391 Streptococcuspneumoniae/mitis/pseudopneumoniae22.9306974 Neisseriaelongata18.9441668 Streptococcussanguinis16.5218336 Lautropiamirabilis13.9148381 Streptococcuspneumoniae/mitis/pseudopneumoniae10.4822942 Streptococcusdentisani4.88811645 Veillonelladispar32.6417554 Streptococcuspneumoniae/mitis/pseudopneumoniae19.0853791 Streptococcussanguinis4.07343037 Fusobacteriumnucleatum51.1079731 Streptococcuspneumoniae/mitis/pseudopneumoniae14.42537482 Fusobacteriumnucleatum6.14816424 Gemellahaemolysans2.67217032 Corynebacteriummatruchotii18.9441668 Streptococcusdentisani10.4497067 Veillonelladispar7.9404736 Veillonelladispar13.7736259 Prevotellaoris13.0241147 Haemophilusparainfluenzae3.67151857 Neisseriaperflava2.60699544 Granulicatellaadiacens8.99413426 Veillonelladispar9.17879644 Neisseriamucosa/macacae2.23767108 Capnocytophagaochracea30.4149468 Streptococcussanguinis0.988485773 Corynebacteriummatruchotii4.59482946 Rothiadentocariosa2.57440799 Selenomonasnoxia8.88550945 Veillonelladispar8.27721051 Capnocytophagasputigena6.61525092 Neisseriamucosa/macacae7.23441234 Fusobacteriumnucleatum11.0905931 Streptococcusdentisani2.08559635 Streptococcusinfantis2.18335868 Campylobactergracilis04.86639148 Streptococcusgordonii5.92005214 Lautropiamirabilis1.70540952 Eikenellacorrodens4.55137954 Neisseriaoralis0.065174894 Actinomycesdentalis3.22615685 Streptococcussanguinis1.30349772 Corynebacteriummatruchotii5.10536607 Streptococcusinfantis4.55137954 Streptococcussanguinis4.63827938 Streptococcusdentisani2.72648273 Anaeroglobusgeminatus10.3845318 Neisseriaperflava0.98848577 Neisseriamucosa/macacae2.04214643 Streptococcusdentisani4.55137954 Streptococcusdentisani3.01976972 Streptococcusdentisani0.87986096 Aggregatibacteractinomycetemcomitans2.1724962 Prevotellaintermedia0.065174895 Atopobiumparvulum3.19356941 Streptococcusdentisani1.29263524 Leptotrichiahofstadii3.37823159 Prevotelladenticola4.24723007 Lautropiamirabilis2.54182055 Prevotellahisticola1.66195959 Dialisterinvisus9.93917011 Haemophilusparaphrohaemolyticus0.69519878 Streptococcuscristatus1.29263524 Streptococcuscristatus4.21464262 Granulicatellaadiacens2.56354551 Granulicatellaadiacens0.57571149 Parvimonasmicra1.86834673 Atopobiumrimae0.05431246 Selenomonassputigena3.10666956 Streptococcusvestibularis0.64088638 Lachnoanaerobaculumsaburreum2.52009559 Prevotellamelaninogenica3.91049316 Granulicatellaadiacens1.98783402 Granulicatellaadiacens1.57505974 Solobacteriummoorei3.93221812 Fusobacteriumnucleatum0.6300239 Neisseriacinerea1.19487291 Corynebacteriummatruchotii0.4562242 Streptococcussanguinis2.46578318 Haemophilusparainfluenzae0.48881164 Johnsonellaignava1.82489681 Actinomycesoris0.043449927 Oribacteriumasaccharolyticum2.46578318 Streptococcusinfantis0.59743645 Campylobactergracilis2.46578318 Prevotellaoris1.93352162 Capnocytophagagingivalis1.42298501 Veillonellaatypica0.81468607 Atopobiumrimae2.84597002 Granulicatellaelegans0.54312405 Streptococcussanguinis1.0862481 Gemellamorbillorum0.30414947 Neisseriamucosa/macacae2.4006083 Streptococcusinfantis0.4562242 [Eubacterium]yurii1.65109711 Kingellaoralis0.032587448 Actinomycesnaeslundii2.04214643 Rothiamucilaginosa0.55398653 Actinomycesdentalis2.44405822 Atopobiumrimae1.60764719 Neisseriaoralis1.20573539 Neisseriabacilliformis0.6843363 Prevotellaloescheii2.33543341 Porphyromonaspasteri0.35846187 Rothiamucilaginosa0.58657397 Actinobaculumschaalii30.27156202 Abiotrophiadefectiva1.73799696 Streptococcuscristatus0.4019118 Campylobactershowae1.28177276 Leptotrichiahongkongensis0.032587449 Actinomycesviscosus1.9443841 Streptococcusgordonii0.39104932 Prevotellaoulorum2.30284597 Streptococcussanguinis1.59678471 Rothiadentocariosa0.87986096 Leptotrichiawadei0.60829894 Prevotellahisticola1.27091028 Caenispirillumdeserti70.24983706 Streptococcusgordonii0.55398653 Neisseriamucosa/macacae0.24983706 Rothiadentocariosa1.66195959 Capnocytophagasputigena0.34759939 Treponemasocranskii0.9124484 Streptococcusvestibularis0.0325874410 Selenomonasnoxia1.85748425 Eggerthiacatenaformis0.31501195 Veillonelladispar2.20508364 Campylobacterconcisus1.18401043 Fusobacteriumnucleatum0.84727352 Megasphaeramicronuciformis0.49967413 Prevotellamicans1.10797306 Gemellahaemolysans0.21724962 Granulicatellaadiacens0.52139909 Kingellaoralis0.23897458 Porphyromonaspasteri0.66261134 Streptococcuspneumoniae/mitis/pseudopneumoniae0.33673691 Brachymonasdenitrificans0.59743645 Cutibacteriumacnes0.0325874411 Klebsiellapneumoniae0-9.1353465 Veillonelladispar-13.502064 Alloprevotellatannerae-22.050836 Klebsiellapneumoniae0-22.800348 Streptococcuspneumoniae/mitis/pseudopneumoniae-29.165761 Streptococcuspneumoniae/mitis/pseudopneumoniae-15.58766 Streptococcuspneumoniae/mitis/pseudopneumoniae-48.099066 Rothiamucilaginosa-5.3334782 Streptococcuspneumoniae/mitis/pseudopneumoniae-15.479035 Streptococcuspneumoniae/mitis/pseudopneumoniae-36.019987 Campylobactergracilis0-16.445796 Fusobacteriumnucleatum-1.8574842 Veillonelladispar-32.891592 Gemellahaemolysans-2.878557512 Fusobacteriumnucleatum-6.5392136 Neisseriamucosa/macacae-10.460569 Leptotrichiashahii-11.459917 Fusobacteriumnucleatum-9.4938084 Prevotellaloescheii-7.9078862 Streptococcusgordonii-4.855529 Streptococcusvestibularis-12.296328 Veillonelladispar-2.7264827 Rothiadentocariosa-0.4236368 Streptococcusvestibularis-8.9506843 Prevotellaloescheii-7.8644362 Veillonelladispar-1.6836846 Prevotellamelaninogenica-10.525744 Neisseriacinerea-1.499022413 Neisseriaoralis-6.3219639 Fusobacteriumnucleatum-6.5609385 Magnetovibrioblakemorei-6.1807517 Prevotellapleuritidis-8.2554856 Fusobacteriumnucleatum-4.6600043 Magnetovibrioblakemorei-2.9545948 Granulicatellaadiacens-3.9539431 Streptococcusvestibularis-2.302846 Lautropiamirabilis-0.3801868 Gemellahaemolysans-4.6165544 Neisseriaoralis-5.5833152 Neisseriaperflava-1.1188355 Streptococcusgordonii-8.3641104 Campylobactergracilis0-1.390397614 Lautropiamirabilis-5.8222898 Kingelladenitrificans-6.1264393 Fusobacteriumnucleatum-4.5296546 Fusobacteriumnucleatum-5.9200521 Neisseriamucosa/macacae-2.3137085 Fusobacteriumnucleatum-2.5309581 Streptococcussanguinis-3.704106 Rothiadentocariosa-0.8038236 Abiotrophiadefectiva-0.3584619 Rothiadentocariosa-3.7366935 Lautropiamirabilis-3.2913317 Porphyromonascatoniae-0.6300239 Streptococcuspneumoniae/mitis/pseudopneumoniae-6.1155768 Neisseriaperflava-1.336085215 Fusobacteriumnucleatum-4.1277428 Klebsiellapneumoniae0-4.7686292 Selenomonassputigena-3.975668 Campylobactergracilis-4.7469042 Prevotellaoris-1.4772974 Gemellahaemolysans-1.6619596 Actinomycesviscosus-2.2050836 Streptococcusinfantis-0.6734738 Granulicatellaelegans-0.3475994 Streptococcussanguinis-2.552683 Fusobacteriumnucleatum-3.0306322 Porphyromonaspasteri-0.4670867 Streptococcusintermedius-3.3890941 Streptococcusgordonii-1.053660716 Caenispirillumdeserti-1.7705844 Abiotrophiadefectiva-3.8235933 Treponemamedium-3.2370193 Leptotrichiagoodfellowii-3.432544 Parvimonasmicra-1.2709103 Prevotelladenticola-1.3686726 Gemellamorbillorum-1.2709103 Streptococcussanguinis-0.586574 Leptotrichiahongkongensis-0.3258744 Neisseriaperflava-0.3584619 Leptotrichiahofstadii-1.5967847 Prevotellaloescheii-0.4453617 Leptotrichiawadei-3.2370193 Lautropiamirabilis-0.83641117 Leptotrichiahongkongensis-1.7705844 Prevotellamelaninogenica-3.725831 Prevotellaloescheii-3.0849446 Paludibacterpropionicigenes-2.4440582 Streptococcusdentisani-1.1948729 Prevotellahisticola-1.2600478 Veillonelladispar-1.2274604 Campylobactergracilis0-0.5757115 Fusobacteriumnucleatum-0.3150119 Rothiamucilaginosa-0.3150119 Corynebacteriummatruchotii-1.5098849 Alloprevotellatannerae-0.4453617 Leptotrichiagoodfellowi-3.2044319 Chryseobacteriumcarnis-0.640886418 Eikenellacorrodens-1.5316098 Leptotrichiashahii-2.84597 Caenispirillumdeserti-2.6721703 Fusobacteriumnucleatum-2.3354334 Gemellahaemolysans-0.7603737 Fusobacteriumnucleatum-1.0319357 Streptococcusinfantis-1.1405605 Leptotrichiawadei-0.5105366 Eggerthiacatenaformis-0.1846622 Lautropiamirabilis-0.2281121 Leptotrichiashahii-1.3686726 Alloprevotellarava-0.4344992 Atopobiumparvulum-2.1942212 Rothiamucilaginosa-0.630023919 Campylobactergracilis-1.3360852 Lautropiamirabilis-2.7807951 Prevotellaveroralis-2.4006083 Capnocytophagaochracea-1.9661091 Alloprevotellarava-0.5974365 Caenispirillumdeserti-1.0319357 Streptococcusgordonii-1.0753856 Stomatobaculumlongum-0.4996741 Actinomycesnaeslundii-0.1737997 Streptococcusparasanguinis-0.1194873 Corynebacteriummatruchotii-1.2491853 Prevotellamelaninogenica-0.293287 [Clostridium]amygdalinum-2.0530089 Porphyromonaspasteri-0.575711520 Leptotrichiawadei-1.1948729 Capnocytophagagingivalis-2.1507712 Selenomonassputigena-1.987834 Streptococcuscristatus-1.6402346 Caenispirillumdeserti-0.4996741 Rothiadentocariosa-0.9450358 Streptococcusdentisani-0.564849 Chryseobacteriumcarnis-0.4236368 Capnocytophagagingivalis-0.1629372 Eggerthiacatenaformis-0.0977623 Prevotellaintermedia-0.7603737 Neisseriaoralis -0.293287 Atopobiumrimae-1.737997 Prevotelladenticola-0.4670867Tooth2:TP2-TP1(25B:t1-t0)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Porphyromonaspasteri13.7410385 Streptococcuspneumoniae/mitis/pseudopneumoniae30.2194221 Eggerthiacatenaformis8.75515968 Streptococcuspneumoniae/mitis/pseudopneumoniae27.8079513 Streptococcussanguinis46.4914186 Streptococcuspneumoniae/mitis/pseudopneumoniae30.7190962 Gemellahaemolysans5.37692809 Abiotrophiadefectiva15.1857484 Porphyromonaspasteri10.7647187 Streptococcusintermedius21.4208125 Streptococcuspneumoniae/mitis/pseudopneumoniae63.4912014 Neisseriamucosa/macacae25.65718012 Streptococcuspneumoniae/mitis/pseudopneumoniae11.4164675 Streptococcusgordonii3.12839453 Fusobacteriumnucleatum8.61394743 Veillonellaratti5.6593526 Alloprevotellarava6.95198783 Streptococcussanguinis5.70280252 Granulicatellaadiacens3.23701934 Streptococcussanguinis7.49511188 Streptococcusintermedius8.84205953 Klebsiellapneumoniae015.7940474 Alloprevotellarava11.9270041 Veillonelladispar18.95502933 Neisseriamucosa/macacae9.0267217 Actinomycesodontolyticus2.60699544 Leptotrichiashahii5.44210298 Fusobacteriumperiodonticum3.43254399 Klebsiellapneumoniae04.1168803 Haemophilusparainfluenzae3.7692809 Abiotrophiadefectiva2.23767108 Rothiadentocariosa6.09385184 Capnocytophagasputigena6.76732566 Campylobactergracilis1.62937215 Gemellahaemolysans7.60373669 Porphyromonaspasteri4.736041714 Porphyromonaspasteri7.06061264 Fusobacteriumnucleatum1.05366066 Klebsiellapneumoniae02.47664567 Neisseriacinerea2.11818379 Streptococcusdentisani4.07343037 Aggregatibacteraphrophilus3.57375625 Veillonellaratti0.99934825 Neisseriaelongata4.47534217 Fusobacteriumnucleatum6.33282642 Parvimonasmicra0.858136 Veillonellaratti6.50662611 Fusobacteriumnucleatum2.183358685 Alloprevotellarava3.83445579 Veillonellaratti0.4562242 Paludibacterpropionicigenes2.16163372 Gemellahaemolysans1.56419726 Streptococcusintermedius2.8568325 Abiotrophiadefectiva2.20508364 Streptococcusdentisani0.96676081 Lautropiamirabilis2.88941994 Neisseriaelongata3.09580708 Porphyromonaspasteri0.69519878 Campylobactergracilis01.06452314 Actinomycesodontolyticus2.053008916 Leptotrichiashahii3.49771888 Porphyromonaspasteri0.31501195 Capnocytophagaleadbetteri2.08559635 Granulicatellaelegans1.30349772 Gemellamorbillorum1.29263524 Veillonelladispar1.67282207 Streptococcusvestibularis0.94503585 Aggregatibacteraphrophilus1.50988486 Gemellamorbillorum2.09645883 Prevotellaloescheii0.54312405 Chryseobacteriumpiscicola0.98848577 Porphyromonaspasteri2.042146437 Streptococcusdentisani2.75907017 Granulicatellaelegans0.27156202 Streptococcuspneumoniae/mitis/pseudopneumoniae2.06387139 Rothiadentocariosa0.86899848 Porphyromonascatoniae1.18401043 Streptococcusdentisani1.40126005 Granulicatellaelegans0.70606126 Streptococcusdentisani1.42298501 Eggerthiacatenaformis1.65109711 Leptotrichiagoodfellowii0.53226157 Streptococcusdentisani0.28242451 Alloprevotellarava1.955246588 Streptococcussanguinis2.02042146 Neisseriamucosa/macacae0.26069954 Neisseriaelongata1.73799696 Campylobactergracilis00.80382359 Porphyromonasendodontalis0.93417337 Neisseriamucosa/macacae1.24918531 Novosphingobiumcolocasiae/soli/panipatense0.54312405 Granulicatellaadiacens0.71692375 Leptotrichiashahii1.3686726 Eubacteriumbrachy0.52139909 Haemophilusinfluenzae0.1737997 Capnocytophagaleadbetteri1.151422999 Fusobacteriumnucleatum1.41212253 Haemophilusparainfluenzae0.2281121 Leptotrichiawadei1.46643493 Haemophilusinfluenzae0.77123615 Fusobacteriumnucleatum0.858136 Fusobacteriumnucleatum1.20573539 Micrococcusluteus0.33673691 Actinomycesodontolyticus0.51053661 Corynebacteriummatruchotii1.35781012 Actinomycesodontolyticus0.48881164 Actinomycesnaeslundii0.08689985 Aggregatibacteraphrophilus1.0210732110 Streptobacillusnotomytis1.18401043 Streptococcuscristatus0.16293721 Tannerellaforsythia1.40126005 Novosphingobiumcolocasiae/soli/panipatense0.28242451 Gemellahaemolysans0.67347382 Bergeyellazoohelcum1.1405605 Klebsiellapneumoniae0.29328699 Haemophilusparainfluenzae0.46708668 Leptotrichiashahii1.29263524 Veillonelladispar0.41277428 Actinobaculumschaalii0.08689985 Granulicatellaadiacens0.9558983311 Streptococcusinfantis-6.1807517 Veillonelladispar-19.758853 Magnetovibrioblakemorei-14.436237 Sphingomonasphyllosphaerae/Erythrobacterflavus-5.6050402 Leptotrichiashahii-13.708451 Lautropiamirabilis-47.349555 Streptococcuspneumoniae/mitis/pseudopneumoniae-12.404953 Streptococcuscristatus-19.096242 Veillonelladispar-18.726917 Streptococcuspneumoniae/mitis/pseudopneumoniae-23.408647 Veillonelladispar-19.356941 Streptococcuspneumoniae/mitis/pseudopneumoniae-20.16076512 Actinomycesdentalis-4.5405171 Streptococcussanguinis-7.6363241 Actinomycesdentalis-5.9526396 Caenispirillumdeserti-3.3673691 Prevotellaloescheii-12.948077 Microlunatussoli-3.5194438 Haemophilusparainfluenzae-2.1399088 Streptococcuspneumoniae/mitis/pseudopneumoniae-11.123181 Lautropiamirabilis-11.459917 Streptococcussanguinis-5.9417771 Leptotrichiawadei-9.6132957 Lautropiamirabilis-17.05409513 Veillonelladispar-4.3775798 Streptococcusdentisani-6.6152509 Veillonelladispar-5.7897024 Veillonelladispar-3.0958071 Veillonelladispar-8.3532479 Capnocytophagahaemolytica-1.1948729 Alloprevotellarava-1.9661091 Campylobactergracilis0-2.3354334 Streptococcussanguinis-10.319357 Gemellahaemolysans-2.281121 Caenispirillumdeserti-6.2459266 Streptococcussanguinis-7.505974414 Tannerellaforsythia-3.6606561 Streptococcusinfantis-0.6843363 Leptotrichiashahii-5.3769281 Lautropiamirabilis-2.9871823 Capnocytophagaleadbetteri-7.1257875 Cardiobacteriumhominis-0.7277862 Veillonelladispar-0.3041495 Gemellahaemolysans-1.8248968 Prevotellaloescheii-6.2133391 Rothiadentocariosa-2.1942212 Prevotellamelaninogenica-5.3117532 Prevotellaintermedia-2.748207715 Sphingomonasphyllosphaerae/Erythrobacterflavus-3.1827069 Prevotellamelaninogenica-0.4888116 Prevotelladenticola-4.7034543 Streptococcussanguinis-2.7699327 Fusobacteriumnucleatum-6.1047143 Leptotrichiagoodfellowii-0.6734738 Streptococcusinfantis-0.1412123 Granulicatellaelegans-1.6293721 Granulicatellaadiacens-2.8242451 Haemophilusinfluenzae-1.9769715 Streptococcusgordonii-4.5296546 Actinomycesnaeslundii-2.161633716 [Clostridium]methylpentosum-3.1501195 Selenomonassputigena-0.3801868 Prevotellahisticola-3.8561808 Fusobacteriumnucleatum-2.6069954 Caenispirillumdeserti-4.0734304 Actinobaculumschaalii9-0.4996741 Streptococcussanguinis-0.0760374 Bergeyellazoohelcum-0.6517489 Actinomycesodontolyticus-2.1507712 Lautropiamirabilis-1.5316098 Neisseriamucosa/macacae-3.4434065 Kingellaoralis -1.803171817 Selenomonasinfelix-2.9437323 Capnocytophagasputigena-0.271562 Alloprevotellatannerae-1.422985 Granulicatellaadiacens-2.574408 Lautropiamirabilis-2.0312839 Prevotellaloescheii-0.4996741 Porphyromonaspasteri-0.0760374 Kingellaoralis -0.4996741 Neisseriamucosa/macacae-2.0964588 Granulicatellaadiacens-1.3034977 Selenomonassputigena-2.9545948 Actinomycesoris-1.249185318 Gemellamorbillorum-2.8568325 Actinomycesviscosus-0.271562 Olsenellauli -1.4121225 Prevotellaloescheii-2.3571584 Actinomycesodontolyticus-1.9009342 Tessaracoccusdefluvii-0.4562242 Actinomycesodontolyticus-0.0651749 Porphyromonaspasteri-0.4127743 Klebsiellapneumoniae0-0.7277862 Haemophilusparainfluenzae-1.1622855 Atopobiumparvulum-2.2919835 Neisseriaperflava-1.227460419 Leptotrichiawadei-2.8133826 Atopobiumrimae-0.1955247 Caenispirillumdeserti-1.3578101 Qipengyuaniasediminis/Altererythrobactermarinus-1.987834 Neisseriamucosa/macacae-1.4664349 Rothiadentocariosa-0.3367369 Porphyromonaspasteri-0.0543124 Corynebacteriummatruchotii-0.3693244 Streptococcusdentisani-0.7277862 Alloprevotellarava-0.8146861 Selenomonassputigena-2.009559 Pseudorhodoferaxcaeni-1.021073220 Rothiamucilaginosa0-2.3137085 Rothiadentocariosa-0.1955247 Megasphaeramicronuciformis-1.2709103 Eggerthiacatenaformis-1.3252227 [Clostridium]xylanolyticum-1.3903976 Neisseriaoralis-0.2063871 Haemophilushaemolyticus-0.0543124 Corynebacteriumdurum-0.3584619 Eikenellacorrodens-0.586574 Streptococcusdentisani-0.5322616 Selenomonasnoxia-1.7814469 Gemellahaemolysans-0.9124484Tooth2:TP3-TP2(21B:t2-t1)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Fusobacteriumnucleatum18.1403433 Veillonelladispar28.6660873 Streptococcusintermedius16.011297 Magnetovibrioblakemorei11.7314795 Neisseriaelongata9.85227026 Streptococcuspneumoniae/mitis/pseudopneumoniae11.6337171 Prevotellaloescheii16.4023463 Lautropiamirabilis62.6439279 Granulicatellaelegans5.62676515 Streptococcuspneumoniae/mitis/pseudopneumoniae32.4245058 Veillonelladispar10.2215946 Streptococcuspneumoniae/mitis/pseudopneumoniae30.6973713 Veillonelladispar11.3838801 Lautropiamirabilis13.48033892 Veillonelladispar7.14751249 Streptococcusdentisani13.6541386 Caenispirillumdeserti14.588312 Actinomycesdentalis5.74625244 Streptococcussanguinis7.4842494 Alloprevotellarava5.04019118 Leptotrichiashahii10.840756 Microlunatussoli3.28046926 Alloprevotellarava2.67217032 Streptococcuscristatus20.8016511 Streptococcuspneumoniae/mitis/pseudopneumoniae8.95068434 Streptococcussanguinis3.4868564 Leptotrichiawadei6.84336302 Streptococcussanguinis9.504670873 Streptococcusinfantis6.14816424 Streptococcussanguinis5.19226591 Actinomycesnaeslundii12.2528786 Prevotelladenticola4.63827938 Streptococcuspneumoniae/mitis/pseudopneumoniae7.24527482 Lautropiamirabilis4.8012166 Fusobacteriumnucleatum7.9404736 Tessaracoccusdefluvii3.20443189 Streptococcusdentisani1.55333478 Campylobactergracilis01.62937215 Prevotellaloescheii6.58266348 Streptococcusintermedius2.78079513 Caenispirillumdeserti5.39865305 Prevotellaintermedia2.911144914 Actinomycesdentalis4.52965457 Actinomycesodontolyticus1.27091028 Eikenellacorrodens8.9724093 Fusobacteriumnucleatum3.81273083 Pseudopropionibacteriumpropionicum6.21333913 Sphingomonasphyllosphaerae/Erythrobacterflavus4.59482946 Capnocytophagaleadbetteri4.56224202 Leptotrichiashahii2.34629589 Granulicatellaadiacens1.22746035 Rothiadentocariosa1.3686726 Fusobacteriumnucleatum2.91114491 Haemophilusinfluenzae2.21594612 Neisseriamucosa/macacae3.44340647 Fusobacteriumnucleatum2.845970025 Tannerellaforsythia3.67151857 Fusobacteriumnucleatum0.38018683 Actinomycesnaeslundii4.24723007 Prevotellahisticola2.95459483 Streptococcusdentisani5.32261569 Porphyromonaspasteri1.28177276 Caenispirillumdeserti4.13860526 Neisseriaoralis1.93352162 Haemophilusparainfluenzae1.02107321 Granulicatellaelegans0.98848577 Neisseriaelongata2.52009559 Rothiadentocariosa2.07473387 Selenomonassputigena2.66130784 Actinomycesnaeslundii2.096458836 Sphingomonasphyllosphaerae/Erythrobacterflavus3.33478166 Selenomonassputigena0.38018683 Actinomycesviscosus2.52009559 Leptotrichiashahii2.75907017 Lautropiamirabilis3.67151857 Neisseriaelongata1.24918531 Streptococcuspneumoniae/mitis/pseudopneumoniae2.63958288 Capnocytophagahaemolytica1.27091028 Veillonellaratti0.47794916 Bergeyellazoohelcum0.87986096 Granulicatellaadiacens2.18335868 Gemellahaemolysans1.90093417 Atopobiumparvulum2.04214643 Kingellaoralis1.781446887 [Clostridium]methylpentosum3.10666956 Prevotellamelaninogenica0.35846187 Actinomycesoris2.48750815 Olsenellauli 1.29263524 Klebsiellapneumoniae03.64979361 Streptococcussanguinis1.16228547 Lautropiamirabilis1.9986965 Cardiobacteriumhominis0.94503585 Porphyromonaspasteri0.30414947 Granulicatellaadiacens0.71692375 Actinomycesodontolyticus2.11818379 Granulicatellaadiacens1.32522268 Selenomonassputigena1.79230936 Actinomycesoris1.151422998 Selenomonasinfelix2.95459483 Streptococcusinfantis0.33673691 Lautropiamirabilis2.08559635 Megasphaeramicronuciformis0.89072344 Veillonelladispar2.86769498 Selenomonassputigena1.1405605 [Clostridium]xylanolyticum1.79230936 Rothiadentocariosa0.74951119 Actinomycesodontolyticus0.06517489 Gemellahaemolysans0.6300239 Neisseriamucosa/macacae2.02042146 Haemophilusparainfluenzae0.69519878 Selenomonasnoxia1.59678471 Prevotellaloescheii1.097110589 Leptotrichiawadei2.84597002 Capnocytophagasputigena0.23897458 Corynebacteriumdurum1.82489681 Selenomonassputigena0.84727352 Fusobacteriumnucleatum1.24918531 Porphyromonaspasteri0.96676081 Capnocytophagasputigena1.50988486 Leptotrichiagoodfellowii0.64088638 Haemophilushaemolyticus0.0543124 Streptococcusdentisani0.4562242 Streptococcusintermedius1.57505974 Alloprevotellarava0.58657397 Selenomonasinfelix1.30349772 Leptotrichiashahii1.0427981810 Gemellamorbillorum2.42233326 Atopobiumrimae0.19552466 Actinomycesmassiliensis1.66195959 Porphyromonasendodontalis0.81468607 Eikenellacorrodens0.97762329 Granulicatellaadiacens0.96676081 Actinomycesodontolyticus1.40126005 Actinobaculumschaalii90.48881164 Prevotellamelaninogenica0.04344992 Kingellaoralis0.35846187 Gemellamorbillorum1.42298501 Porphyromonaspasteri0.48881164 Lachnoanaerobaculumsaburreum1.24918531 Pseudorhodoferaxcaeni1.0210732111 Streptococcuspneumoniae/mitis/pseudopneumoniae-54.02998 Streptococcuspneumoniae/mitis/pseudopneumoniae-28.742125 Veillonelladispar-44.48186 Caenispirillumdeserti-23.169672 Fusobacteriumnucleatum-18.39018 Prevotellaloescheii-7.8101238 Veillonelladispar-39.75668 Streptococcuspneumoniae/mitis/pseudopneumoniae-54.844667 Streptococcuspneumoniae/mitis/pseudopneumoniae-5.9417771 Streptococcussanguinis-11.861829 Streptococcussanguinis-12.459266 Neisseriaperflava-9.6458831 Streptococcusgordonii-41.092766 Streptococcuspneumoniae/mitis/pseudopneumoniae-37.60590912 Streptococcuscristatus-5.8548773 Streptococcusgordonii-24.136433 Streptococcussanguinis-5.3443406 Leptotrichiawadei-4.3667174 Alloprevotellarava-7.8752987 Eggerthiacatenaformis-6.593526 Streptococcussanguinis-8.1359983 Fusobacteriumnucleatum-6.7456007 Veillonelladispar-2.0204215 Lautropiamirabilis-6.1481642 Lautropiamirabilis-10.341082 Fusobacteriumnucleatum-7.7666739 Streptococcuspneumoniae/mitis/pseudopneumoniae-14.077775 Rothiadentocariosa-3.204431913 Campylobactergracilis0-5.0401912 Rothiadentocariosa-0.7495112 Fusobacteriumnucleatum-4.0517054 Alloprevotellatannerae-3.9322181 Campylobactergracilis0-3.139257 Caenispirillumdeserti-3.3565066 Streptococcusgordonii-7.6797741 Streptococcussanguinis-4.8446665 Streptococcuscristatus-1.0536607 Neisseriamucosa/macacae-5.5398653 Capnocytophagasputigena-7.2887247 Paludibacterpropionicigenes-6.2676515 Streptococcussanguinis-1.6185097 Campylobactergracilis0-2.031283914 Streptococcusdentisani-4.6165544 Gemellahaemolysans-0.2498371 Alloprevotellarava-3.7149685 Prevotellaloescheii-1.9552466 Leptotrichiashahii-2.6721703 Leptotrichiashahii-3.2370193 Alloprevotellarava-4.7251792 Streptococcusinfantis-3.2044319 Gemellahaemolysans-0.7495112 Streptococcusvestibularis-4.8446665 Actinomycesnaeslundii-4.7143167 Capnocytophagaleadbetteri-2.4875081 Prevotelladenticola-0.4344992 Streptococcusdentisani-1.292635215 Neisseriamucosa/macacae-4.2146426 Actinomycesviscosus-0.1412123 Streptococcusgordonii-3.6280687 Klebsiellapneumoniae0-1.3252227 Gemellamorbillorum-2.552683 Fusobacteriumnucleatum-2.8351075 Actinomycesmassiliensis-1.6185097 Alloprevotellarava-1.6619596 Streptococcussanguinis-0.543124 Leptotrichiashahii-2.9654573 Actinomycesmassiliensis-3.0958071 Caenispirillumdeserti-2.4006083 Leptotrichiashahii-0.3258744 Neisseriamucosa/macacae-1.064523116 Porphyromonaspasteri-3.0306322 Streptococcusparasanguinis-0.1194873 Neisseriamucosa/macacae-2.9871823 Prevotellaoulorum-1.1405605 Granulicatellaelegans9-1.7271345 Qipengyuaniasediminis/Altererythrobactermarinus-1.8357593 Porphyromonaspasteri-1.40126 Gemellamorbillorum-1.2057354 Rothiamucilaginosa-0.4996741 Eggerthiacatenaformis-2.5635455 Capnocytophagagingivalis-2.6830328 Klebsiellapneumoniae0-2.0964588 Granulicatellaadiacens-0.3041495 Porphyromonaspasteri-0.58657417 Granulicatellaelegans-1.9443841 Neisseriamucosa/macacae-0.0760374 Streptococcuspneumoniae/mitis/pseudopneumoniae-2.3571584 Prevotellasalivae-0.9667608 Campylobactershowae-1.6836846 Caenispirillumdeserti-1.1948729 Streptococcusdentisani-0.8689985 Streptococcusdentisani-0.9450358 Streptococcusinfantis-0.4670867 Neisseriaperflava-2.2376711 Actinomycesnaeslundii-1.6293721 Fusobacteriumnucleatum-1.1622855 Magnetovibrioblakemorei-0.2824245 Corynebacteriumdurum-0.54312418 Streptococcusgordonii-1.5967847 Streptococcusparasanguinis-0.0760374 Actinomycesdentalis-1.3252227 Leptotrichiashahii8-0.8472735 Leptotrichiashahii-1.3360852 Aggregatibacteraphrophilus-1.1405605 Kingellaoralis -0.7603737 Klebsiellapneumoniae0-0.8472735 Streptococcusgordonii-0.271562 Veillonelladispar-1.9769715 Rothiadentocariosa-0.9667608 Leptotrichiashahii-1.0645231 Streptococcusdentisani-0.271562 Neisseriacinerea-0.401911819 Porphyromonaspasteri-1.2709103 Campylobactergracilis0-0.0543124 Actinomycesodontolyticus-1.0319357 Prevotellaveroralis-0.6300239 Oscillibacterruminantium-1.1840104 Magnetovibrioblakemorei-1.0645231 Leptotrichiahongkongensis-0.293287 Veillonelladispar-0.8146861 Neisseriaperflava-0.1520747 Abiotrophiadefectiva-1.9226591 Haemophilusparainfluenzae-0.4019118 Alloprevotellatannerae-0.9015859 Prevotellasalivae-0.2172496 Chryseobacteriumcarnis-0.347599420 Gemellahaemolysans-1.0862481 Actinomycesoris-0.0434499 Prevotellamelaninogenica-0.9450358 Veillonellaatypica-0.5974365 Paludibacterpropionicigenes-0.9450358 Veillonelladispar-0.7820986 Neisseriaelongata-0.2281121 Parvimonasmicra-0.6843363 Neisseriaelongata-0.1520747 Rothiamucilaginosa-1.9009342 Qipengyuaniasediminis/Altererythrobactermarinus-0.3693244 Neisseriaelongata-0.9015859 OTU_233 -0.1955247 Bergeyellazoohelcum-0.3041495Tooth3:TP2-TP1(21B:t1-t0)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Porphyromonaspasteri19.9652401 Veillonelladispar50.7386487 Streptococcuspneumoniae/mitis/pseudopneumoniae87.2474473 Streptococcussanguinis20.4214643 Streptococcuspneumoniae/mitis/pseudopneumoniae21.5185748 Streptococcuspneumoniae/mitis/pseudopneumoniae54.1168803 Granulicatellaelegans7.85357376 Streptococcuspneumoniae/mitis/pseudopneumoniae20.8125136 Neisseriaoralis23.6041712 Gemellahaemolysans9.92830763 Neisseriaperflava8.73343472 Actinobaculumschaalii52.574408 Veillonelladispar7.02802522 Neisseriamucosa/macacae17.3908321 Actinomycesodontolyticus1.55333478 Streptococcusinfantis0.20638714 Fusobacteriumnucleatum4.14946774 Neisseriamucosa/macacae9.18965892 Streptococcusvestibularis6.26765153 Gemellahaemolysans4.61655442 Granulicatellaelegans7.08233761 Lautropiamirabilis3.04149468 Streptococcusintermedius7.44079948 Streptococcusinfantis5.74625244 Streptococcuspneumoniae/mitis/pseudopneumoniae25.9504671 Alloprevotellarava6.734738213 Veillonelladispar16.2394091 Granulicatellaadiacens1.03193569 Streptococcusdentisani0.13034977 Leptotrichiashahii3.57375625 Streptococcusvestibularis7.62546166 Haemophilusparainfluenzae2.15077124 Haemophilusparainfluenzae1.90093417 Neisseriaperflava0.73864871 Capnocytophagasputigena2.98718227 Veillonelladispar5.33347817 Gemellahaemolysans3.43254399 Gemellahaemolysans4.69259179 Porphyromonaspasteri4.605691944 Fusobacteriumnucleatum10.0803824 Abiotrophiadefectiva0.20638714 Novosphingobiumcolocasiae/soli/panipatense0.11948729 Lautropiamirabilis2.8025201 Streptococcussanguinis3.18270693 Veillonelladispar0.69519878 Streptococcusvestibularis1.24918531 Gemellahaemolysans0.59743645 Leptotrichiashahii1.47729741 Granulicatellaelegans2.86769498 Fusobacteriumnucleatum1.9986965 Actinomycesnaeslundii1.46643493 Streptococcusgordonii2.357158375 Streptococcussanguinis9.26569629 Capnocytophagasputigena0.14121225 Rothiamucilaginosa0.10862481 Porphyromonascatoniae2.32457093 Rothiamucilaginosa2.4549207 Streptococcusgordonii0.51053661 Haemophilusparaphrohaemolyticus1.04279818 Haemophilushaemolyticus0.39104932 Neisseriaelongata0.95589833 Neisseriaperflava1.69454703 Alloprevotellarava0.858136 Streptococcusdentisani0.42363676 Porphyromonaspasteri2.215946126 Streptococcuspneumoniae/mitis/pseudopneumoniae4.77949164 Lautropiamirabilis0.10862481 Granulicatellaelegans0.08689985 Neisseriamucosa/macacae1.5424723 Streptococcusparasanguinis2.36802086 Granulicatellaadiacens0.46708668 Rothiamucilaginosa0.6843363 Granulicatellaadiacens0.09776233 Abiotrophiadefectiva0.858136 Granulicatellaadiacens1.39039757 Veillonelladispar0.71692375 Chryseobacteriumpiscicola0.35846187 Porphyromonaspasteri2.031283957 Abiotrophiadefectiva3.4868564 Actinomycesmassiliensis0.08689985 Campylobactergracilis00.0543124 Capnocytophagasputigena1.50988486 Streptococcusinfantis1.91179665 Streptococcusdentisani0.27156202 Granulicatellaadiacens0.64088638 Leptotrichiashahii0.08689985 Capnocytophagagingivalis0.82554856 Streptococcusinfantis1.34694764 Porphyromonaspasteri0.47794916 Actinomycesviscosus0.33673691 Granulicatellaelegans2.020421468 Streptococcusdentisani2.37888334 Fusobacteriumnucleatum0.08689985 Rothiadentocariosa0.0543124 Corynebacteriummatruchotii1.29263524 Haemophilusparainfluenzae1.80317184 Haemophilushaemolyticus0.07603737 Chryseobacteriumcarnis0.53226157 Haemophilusparaphrohaemolyticus0.06517489 Tessaracoccusdefluvii0.61916142 Fusobacteriumnucleatum1.12969802 Leptotrichiashahii0.33673691 Pseudopropionibacteriumpropionicum0.24983706 Veillonellaratti1.596784719 Lachnoanaerobaculumsaburreum1.15142299 Actinomycesnaeslundii0.04344992 Neisseriacinerea0.04344992 Streptococcusdentisani1.22746035 Streptococcusparasanguinis1.73799696 Porphyromonascatoniae0.0543124 Streptococcusdentisani0.41277428 Corynebacteriummatruchotii0.0543124 Streptococcusdentisani0.53226157 Gemellamorbillorum1.01021073 Streptococcusparasanguinis0.31501195 Caenispirillumdeserti0.16293721 Neisseriamucosa/macacae1.4990223810 Leptotrichiashahii1.07538562 Bergeyellazoohelcum0.03258744 Paracoccusmarinus0.04344992 Porphyromonaspasteri1.17314795 Gemellahaemolysans1.3143602 Campylobacterconcisus0.01086248 Streptococcussanguinis0.16293721 Neisseriaelongata0.0543124 Granulicatellaadiacens0.39104932 Haemophilusparainfluenzae0.47794916 Neisseriamucosa/macacae0.29328699 Haemophilushaemolyticus0.11948729 Abiotrophiadefectiva1.1296980211 Fusobacteriumnucleatum-39.702368 Streptococcusdentisani-21.312188 Caenispirillumdeserti-37.725396 Streptococcuspneumoniae/mitis/pseudopneumoniae-15.316098 Veillonelladispar-23.962633 Lautropiamirabilis-29.350424 Streptococcuspneumoniae/mitis/pseudopneumoniae-18.466218 Neisseriamucosa/macacae-7.2778623 Streptococcuscristatus-10.395394 Streptococcuspneumoniae/mitis/pseudopneumoniae-33.575929 Streptococcussanguinis-7.7775364 Veillonelladispar-38.464045 Streptococcussanguinis-6.126439312 Caenispirillumdeserti-12.752553 Streptococcussanguinis-16.054747 Magnetovibrioblakemorei-3.3999566 Fusobacteriumnucleatum-7.8535738 Streptococcusintermedius-17.29307 Rothiadentocariosa-3.5194438 Veillonellaratti-0.9124484 Streptococcusdentisani-5.0836411 Streptococcuspneumoniae/mitis/pseudopneumoniae-9.7870954 Streptococcusdentisani-1.2274604 Streptococcuspneumoniae/mitis/pseudopneumoniae-6.5066261 Streptococcusgordonii-6.3654139 Gemellahaemolysans-4.692591813 Prevotellapallens-7.2669998 Streptococcusgordonii-5.0945036 Klebsiellapneumoniae0-2.9545948 Prevotellaloescheii-5.420378 Klebsiellapneumoniae0-3.4651314 Streptococcussanguinis-3.4434065 Alloprevotellarava-0.0977623 Porphyromonaspasteri-4.2363676 Streptococcussanguinis-4.6165544 Streptococcussanguinis-1.2165979 Granulicatellaadiacens-2.4766457 Prevotellasalivae-5.8005648 Lautropiamirabilis-3.41081914 Prevotellaoris-5.3334782 Actinomycesviscosus-3.9539431 Leptotrichiawadei-2.84597 Leptotrichiagoodfellowii-3.0197697 Fusobacteriumnucleatum-2.3354334 Campylobactergracilis0-2.552683 Haemophilushaemolyticus-0.0434499 Streptococcussanguinis-4.0842929 Kingellaoralis -2.7482077 Campylobactergracilis0-0.5213991 Lautropiamirabilis-1.4121225 Streptococcussanguinis-4.4970671 Neisseriaperflava-2.8459715 Klebsiellapneumoniae0-3.2587443 Streptococcuspneumoniae/mitis/pseudopneumoniae-2.9220074 Veillonelladispar-2.5852705 Prevotellamelaninogenica-2.5418206 Leptotrichiawadei-1.1948729 Neisseriaoralis-2.0204215 Porphyromonaspasteri-0.0325874 Streptococcuscristatus-3.8670432 Campylobactergracilis0-2.3462959 Rothiadentocariosa-0.4779492 Streptococcusdentisani-1.2165979 Leptotrichiawadei-3.117532 Streptococcuspneumoniae/mitis/pseudopneumoniae-2.476645716 Campylobactergracilis-2.8242451 Actinomycesoris-1.3795351 Actinobaculumschaalii1-2.4657832 Granulicatellaadiacens-1.9009342 Prevotellaoulorum-1.0102107 Neisseriamucosa/macacae-2.009559 Neisseriaelongata-0.0325874 Bergeyellazoohelcum-1.1079731 Porphyromonaspasteri-0.7277862 Porphyromonaspasteri-0.1086248 Rothiadentocariosa-1.1840104 Caenispirillumdeserti-3.0849446 Streptococcusvestibularis-1.672822117 Actinomycesdentalis-2.0747339 Streptococcusinfantis-0.7712362 Selenomonassputigena-1.9986965 Actinomycesgeorgiae-1.8140343 Fusobacteriumnucleatum-0.8038236 Gemellahaemolysans-1.8792092 Neisseriamucosa/macacae-0.021725 Veillonelladispar-0.8038236 Actinomycesviscosus-0.7169237 Neisseriaoralis-0.0868998 Streptococcuscristatus-1.0862481 Atopobiumparvulum-2.6287204 Actinomycesoris-1.4447118 Fusobacteriumnucleatum-1.4338475 Haemophilusparainfluenzae-0.543124 Leptotrichiashahii-1.9117967 Leptotrichiawadei-1.4881599 Granulicatellaadiacens-0.7169237 Leptotrichiahongkongensis-1.4881599 Neisseriacinerea-0.021725 Abiotrophiadefectiva-0.4670867 Actinomycesnaeslundii-0.6191614 Streptococcusvestibularis-0.0651749 Haemophilusparainfluenzae-0.4453617 Selenomonassputigena-2.1724962 Fusobacteriumnucleatum-1.227460419 Capnocytophagasputigena-1.1731479 Alloprevotellatannerae-0.1955247 Selenomonassputigena-1.8140343 Gemellahaemolysans-1.4772974 Megasphaeramicronuciformis-0.6408864 Fusobacteriumnucleatum-1.2274604 Prevotellamelaninogenica-0.0108625 Streptococcusinfantis-0.4019118 Neisseriamucosa/macacae-0.5539865 Streptococcuscristatus-0.0434499 Granulicatellaelegans-0.4344992 Atopobiumrimae-2.1073213 Cutibacteriumacnes-1.205735420 Leptotrichiawadei-1.0971106 Rothiadentocariosa-0.1520747 Prevotelladenticola-1.7271345 Streptococcusgordonii-1.2817728 Prevotellahisticola-0.4670867 Tessaracoccusdefluvii-1.0319357 Streptococcusgordonii-0.0108625 Fusobacteriumnucleatum-0.3367369 Leptotrichiahongkongensis-0.4670867 Actinomycesodontolyticus-0.0325874 Capnocytophagasputigena-0.3693244 Prevotellamelaninogenica-1.7923094 Actinomycesviscosus-1.0862481Tooth3:TP3-TP2(21B:t2-t1)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Fusobacteriumnucleatum38.1816207 Streptococcussanguinis12.1008038 Caenispirillumdeserti26.2111666 Veillonelladispar10.9493808 Veillonelladispar25.6028677 Lautropiamirabilis29.6219857 Streptococcuspneumoniae/mitis/pseudopneumoniae5.9417771 Streptococcuscristatus13.8713882 Streptococcuspneumoniae/mitis/pseudopneumoniae50.5322616 Streptococcuspneumoniae/mitis/pseudopneumoniae59.6458831 Streptococcuspneumoniae/mitis/pseudopneumoniae5.78970237 Veillonelladispar14.9576363 Streptococcussanguinis5.778839892 Caenispirillumdeserti8.66825983 Streptococcusdentisani11.3187052 Streptococcuspneumoniae/mitis/pseudopneumoniae4.82294156 Fusobacteriumnucleatum7.85357376 Streptococcussanguinis19.3026287 Gemellahaemolysans4.00825549 Granulicatellaelegans3.2587443 Neisseriamucosa/macacae7.13665001 Streptococcuscristatus13.0892896 Streptococcusdentisani2.8568325 Streptococcussanguinis3.23701934 Prevotellasalivae5.34434065 Gemellahaemolysans5.159678473 Prevotellapallens5.4312405 Actinomycesviscosus2.2268086 Magnetovibrioblakemorei2.46578318 Leptotrichiagoodfellowii3.50858136 Streptococcusintermedius17.3582446 Rothiadentocariosa3.55203128 Haemophilusparainfluenzae2.61785792 Streptococcusdentisani4.92070389 Granulicatellaadiacens2.83510754 Gemellahaemolysans1.57505974 Granulicatellaadiacens2.30284597 Caenispirillumdeserti2.98718227 Lautropiamirabilis3.193569414 Prevotellaoris3.00890723 Streptococcusgordonii1.85748425 Actinobaculumschaalii12.44405822 Prevotellaloescheii3.23701934 Streptococcusdentisani3.79100587 Neisseriaoralis2.19422116 Veillonellaratti2.25939605 Streptococcuspneumoniae/mitis/pseudopneumoniae2.88941994 Campylobactergracilis02.79165761 Granulicatellaelegans0.33673691 Streptococcusdentisani1.3686726 Leptotrichiawadei2.78079513 Veillonelladispar2.270258535 Klebsiellapneumoniae03.00890723 Veillonelladispar1.82489681 Klebsiellapneumoniae02.2268086 Prevotellamelaninogenica2.18335868 Streptococcuspneumoniae/mitis/pseudopneumoniae3.47599392 Leptotrichiahongkongensis1.5424723 Alloprevotellarava1.64023463 Porphyromonaspasteri1.41212253 Kingellaoralis2.19422116 Granulicatellaadiacens0.24983706 Haemophilusparainfluenzae1.35781012 Atopobiumparvulum2.41147078 Neisseriaperflava2.107321316 Campylobactergracilis2.6287204 Actinomycesoris0.93417337 Prevotelladenticola1.06452314 Actinomycesgeorgiae1.92265914 Klebsiellapneumoniae03.42168151 Campylobactergracilis01.28177276 Streptococcusdentisani0.28242451 Bergeyellazoohelcum1.3143602 Streptococcusdentisani0.96676081 Kingellaoralis0.04344992 Rothiadentocariosa0.74951119 Selenomonassputigena2.16163372 Streptococcusvestibularis1.737996967 Fusobacteriumnucleatum1.35781012 Haemophilusparainfluenzae0.58657397 Selenomonassputigena0.89072344 Leptotrichiawadei0.90158592 Granulicatellaadiacens1.26004779 Tessaracoccusdefluvii1.04279818 Granulicatellaadiacens0.24983706 Neisseriaperflava0.70606126 Porphyromonaspasteri0.72778623 Abiotrophiadefectiva0.04344992 Streptococcuscristatus0.48881164 Atopobiumrimae2.06387139 Actinomycesoris1.422985018 Fusobacteriumnucleatum1.11883554 Alloprevotellatannerae0.19552466 Gemellahaemolysans0.82554856 Neisseriaoralis0.83641104 Neisseriamucosa/macacae0.87986096 Leptotrichiatrevisanii0.74951119 Haemophilusparaphrohaemolyticus0.11948729 Veillonelladispar0.60829894 Bergeyellazoohelcum0.64088638 Streptococcuscristatus0.02172496 Gemellamorbillorum0.43449924 Prevotellaoulorum1.64023463 Cutibacteriumacnes1.281772769 Capnocytophagasputigena1.06452314 Prevotellaintermedia0.10862481 Prevotellapleuritidis0.72778623 Campylobactergracilis0.56484901 Prevotellaoulorum0.87986096 Corynebacteriumdurum0.71692375 Abiotrophiadefectiva0.07603737 Fusobacteriumnucleatum0.46708668 Tessaracoccusdefluvii0.34759939 Oribacteriumasaccharolyticum0.02172496 Granulicatellaelegans0.36932435 Stomatobaculumlongum1.24918531 Actinomycesviscosus1.086248110 Actinobaculumschaalii10.9124484 Caenispirillumdeserti0.07603737 Prevotellahisticola0.72778623 Granulicatellaadiacens0.55398653 Fusobacteriumnucleatum0.80382359 Thioreductormicantisoli0.56484901 Haemophilushaemolyticus0.03258744 Tessaracoccusdefluvii0.43449924 Porphyromonascatoniae0.2281121 Actinomycesviscosus0.01086248 Capnocytophagagingivalis0.27156202 Prevotelladenticola1.10797306 Neisseriamucosa/macacae0.8364110411 Prevotelladenticola-41.005866 Streptococcuspneumoniae/mitis/pseudopneumoniae-27.623289 Leptotrichiawadei-12.643928 Alloprevotellarava-11.568542 Capnocytophagasputigena-25.059744 Streptococcuspneumoniae/mitis/pseudopneumoniae-26.558766 Streptococcussanguinis-1.9335216 Granulicatellaelegans-18.335868 Streptococcussanguinis-50.847274 Rothiadentocariosa-15.685423 Neisseriaperflava-2.84597 Streptococcusgordonii-18.82468 Streptococcuspneumoniae/mitis/pseudopneumoniae-26.11340412 Atopobiumrimae-4.9750163 Granulicatellaelegans4-0.8907234 Leptotrichiashahii-12.231154 Neisseriamucosa/macacae-5.985227 Fusobacteriumnucleatum-11.459917 Streptococcussanguinis-13.121877 Gemellahaemolysans-1.5859222 Alloprevotellarava-5.127091 Lautropiamirabilis-7.8752987 Actinomycesnaeslundii-6.5392136 Streptococcusinfantis-2.0421464 Prevotellamelaninogenica-13.817076 Campylobactergracilis0-6.484901213 Selenomonassputigena-3.9213556 Streptococcusinfantis-0.8472735 Veillonelladispar-10.058657 Streptococcussanguinis-3.6823811 Leptotrichiashahii-10.15642 Fusobacteriumnucleatum-4.8012166 Rothiamucilaginosa-1.44471 Streptococcussanguinis-4.7360417 Abiotrophiadefectiva-4.7794916 Prevotellaintermedia-5.6159027 Veillonelladispar-1.5533348 Streptococcuspneumoniae/mitis/pseudopneumoniae-10.688681 Streptococcusinfantis-0.54312414 Dialisterinvisus-2.9980448 Fusobacteriumnucleatum-0.5757115 Prevotellaloescheii-2.5635455 Streptococcuspneumoniae/mitis/pseudopneumoniae-2.84597 Kingellaoralis -6.4849012 Streptococcusvestibularis-2.6830328 Neisseriaperflava-1.3360852 Veillonellaratti-4.3667174 Actinomycesviscosus-4.6056919 Lautropiamirabilis-3.3239192 Porphyromonaspasteri-1.4990224 Streptococcussanguinis-1.44471 Porphyromonaspasteri-0.532261615 Prevotellahisticola-2.552683 Campylobacterconcisus-0.3584619 Lachnoanaerobaculumsaburreum-1.6402346 Streptococcusdentisani-1.6185097 Streptococcusgordonii-4.8012166 Neisseriamucosa/macacae-2.1833587 Veillonelladispar-1.1948729 Gemellahaemolysans-2.4875081 Actinomycesoris-2.6721703 Fusobacteriumnucleatum-2.574408 Fusobacteriumnucleatum-1.3578101 Leptotrichiagoodfellowi-0.5105366 Streptococcusdentisani-0.401911816 Veillonelladispar-2.1942212 Veillonellaratti-0.2498371 Fusobacteriumnucleatum-1.6185097 Granulicatellaelegans-1.3686726 Leptotrichiahongkongensis-2.4006083 Streptococcusinfantis-1.6728221 Chryseobacteriumcarnis-0.9993483 Actinobaculumschaalii6-0.6300239 Caenispirillumdeserti-1.2600478 Veillonelladispar-1.8574842 Prevotellamelaninogenica-0.7712362 Prevotellamassiliatimonensis-0.3367369 Alloprevotellarava-0.27156217 Neisseriamucosa/macacae-1.7054095 Neisseriaperflava5-0.1955247 Capnocytophagaleadbetteri-1.4664349 Gemellahaemolysans-1.0645231 Capnocytophagaochracea-2.3788833 Streptococcusdentisani-1.5750597 Lautropiamirabilis-0.8689985 Streptococcusgordonii-0.3801868 Veillonelladispar-0.7820986 Eggerthiacatenaformis-1.6293721 Porphyromonaspasteri-0.7603737 Megasphaeramicronuciformis-0.3367369 Prevotellaloescheii-0.130349818 Megasphaeramicronuciformis-1.2926352 Streptococcusanginosus-0.1737997 Prevotellamelaninogenica-1.2600478 Leptotrichiashahii-0.7712362 Selenomonasnoxia-2.0855964 Veillonelladispar-1.2383228 Streptococcusinfantis-0.7820986 Haemophilusparainfluenzae-0.1194873 Corynebacteriummatruchotii-0.7495112 Neisseriaperflava-1.5533348 Corynebacteriumdurum-0.3693244 Gemellahaemolysans-0.2498371 Streptococcusparasanguinis-0.108624819 Qipengyuaniasediminis/Altererythrobactermarinus-1.2817728 Alloprevotellarava-0.1737997 Actinomycesdentalis-1.1188355 Fusobacteriumnucleatum-0.6843363 Campylobactergracilis-1.5316098 Gemellamorbillorum-0.9233109 Rothiadentocariosa-0.6082989 Granulicatellaadiacens-0.1086248 Corynebacteriummatruchotii-0.586574 Actinomycesnaeslundii-1.4664349 Clavibactermichiganensis-0.3367369 Prevotellapallens-0.1520747 Chryseobacteriumcarnis-0.097762320 Stomatobaculumlongum-1.2491853 Gemellahaemolysans-0.1520747 Prevotellasalivae-1.0536607 Eggerthiacatenaformis-0.6626113 [Eubacterium]yurii-1.5207473 Streptococcusintermedius-0.7386487 Streptococcusvestibularis-0.564849 Haemophilusinfluenzae-0.0651749 Leptotrichiahongkongensis-0.4996741 Neisseriaoralis-1.4121225 Lautropiamirabilis-0.3258744 Streptococcusdentisani-0.1086248 Neisseriacinerea-0.0651749Tooth4:TP2-TP1(21L:t1-t0)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Leptotrichiashahii6.94112535 Streptococcuspneumoniae/mitis/pseudopneumoniae6.72387573 Fusobacteriumnucleatum20.4649142 Lautropiamirabilis24.5057571 Kingellaoralis58.1685857 Streptococcuspneumoniae/mitis/pseudopneumoniae17.7492939 Eggerthiacatenaformis32.88073 Streptococcuspneumoniae/mitis/pseudopneumoniae16.8151206 Porphyromonascatoniae9.26569629 Lautropiamirabilis12.9372149 Prevotellaloescheii10.3193569 Streptococcuspneumoniae/mitis/pseudopneumoniae38.12730832 Fusobacteriumnucleatum6.48490115 Streptococcusdentisani5.2574408 Capnocytophagasputigena10.0369324 Streptococcussanguinis7.46252444 Capnocytophagasputigena7.54942429 Neisseriaelongata10.7755811 Altererythrobactermarinus12.013904 Corynebacteriumdurum4.44275473 Fusobacteriumnucleatum6.45231371 Fusobacteriumnucleatum9.4829459 Leptotrichiatrevisanii8.49446013 Porphyromonaspasteri4.431892243 Corynebacteriummatruchotii3.41081903 Lautropiamirabilis4.29067999 Streptococcuspneumoniae/mitis/pseudopneumoniae9.70019552 Granulicatellaadiacens1.47729741 Neisseriabacilliformis2.79165761 Campylobactergracilis03.89963068 Corynebacteriummatruchotii7.34303715 Rothiadentocariosa1.98783402 Leptotrichiashahii5.18140343 Porphyromonascatoniae4.76862915 Porphyromonascatoniae6.47403867 Alloprevotellarava3.443406474 Streptococcuspneumoniae/mitis/pseudopneumoniae3.10666956 Neisseriamucosa/macacae3.9430806 Leptotrichiagoodfellowii5.09450358 Neisseriamucosa/macacae1.26004779 Tessaracoccusdefluvii2.10732131 Capnocytophagasputigena2.82424506 Porphyromonascatoniae5.24657832 Neisseriaoralis1.83575929 Eikenellacorrodens4.98587877 Porphyromonascatoniae4.23636759 Streptococcuspneumoniae/mitis/pseudopneumoniae6.13730176 Fusobacteriumnucleatum2.596132965 Kingelladenitrificans1.95524658 Streptococcuscristatus2.82424506 Leptotrichiashahii4.70345427 Streptococcusdentisani0.73864871 Leptotrichiahongkongensis1.72713448 Leptotrichiahongkongensis2.02042146 Neisseriaelongata5.18140343 Eggerthiacatenaformis1.7705844 Neisseriaperflava4.76862915 Leptotrichiashahii3.74755594 Fusobacteriumnucleatum5.56159027 Porphyromonaspasteri2.215946126 Capnocytophagasputigena1.75972192 Abiotrophiadefectiva2.4006083 Klebsiellapneumoniae04.49706713 Neisseriaperflava0.60829894 Bergeyellazoohelcum1.07538562 Leptotrichiashahii1.96610906 Porphyromonaspasteri4.60569194 Neisseriaelongata1.44470997 Eggerthiacatenaformis4.32326743 Prevotellaloescheii3.23701934 Neisseriaperflava84.56224202 Prevotellamelaninogenica2.183358687 Capnocytophagaleadbetteri1.61850967 Kingellaoralis 1.716272 Streptococcussanguinis3.49771888 Abiotrophiadefectiva0.39104932 Cardiobacteriumhominis0.76037367 Streptococcusdentisani1.4881599 Corynebacteriummatruchotii3.4868564 Abiotrophiadefectiva0.46708668 Lautropiamirabilis4.26895503 Porphyromonascatoniae2.76993265 Eggerthiacatenaformis1.93352162 Leptotrichiashahii1.814034338 Eggerthiacatenaformis1.35781012 Capnocytophagasputigena1.19487291 Leptotrichiashahii2.94373235 Caenispirillumdeserti0.24983706 Rothiadentocariosa0.10862481 Fusobacteriumnucleatum0.90158592 Porphyromonaspasteri2.86769498 Capnocytophagasputigena50.44536172 Porphyromonaspasteri3.92135564 Abiotrophiadefectiva2.53095807 Campylobactergracilis01.66195959 Veillonellaratti1.814034339 Leptotrichiahongkongensis1.3143602 Rothiadentocariosa0.69519878 Pseudopropionibacteriumpropionicum2.86769498 Corynebacteriumdurum0.16293721 Klebsiellapneumoniae10.07603737 Gemellahaemolysans0.82554856 Selenomonasnoxia2.06387139 Actinomycesnaeslundii0.36932435 Capnocytophagasputigena2.53095807 Gemellamorbillorum1.9443841 Gemellahaemolysans1.65109711 Granulicatellaelegans1.3469476410 Chryseobacteriumcarnis1.29263524 Kingelladenitrificans0.64088638 [Eubacterium]yurii2.21594612 Cardiobacteriumhominis0.10862481 Novosphingobiumcolocasiae/soli/panipatense0.02172496 Porphyromonascatoniae0.52139909 Capnocytophagaochracea1.81403433 Capnocytophagaleadbetteri0.32587443 Leptotrichiashahii2.52009559 [Eubacterium]yurii1.79230936 Granulicatellaadiacens1.56419726 Veillonelladispar1.3469476411 Neisseriaoralis-8.6248099 Porphyromonaspasteri-11.16663 Alloprevotellatannerae-17.358245 Eggerthiacatenaformis-4.7469042 Streptococcuspneumoniae/mitis/pseudopneumoniae-15.739735 Lautropiamirabilis-21.518575 Streptococcuspneumoniae/mitis/pseudopneumoniae-87.366935 Neisseriamucosa/macacae-9.4069085 Streptococcuspneumoniae/mitis/pseudopneumoniae-43.482511 Streptococcuspneumoniae/mitis/pseudopneumoniae-22.018249 Veillonelladispar-16.652183 Streptococcussanguinis-49.32652612 Streptococcusintermedius-6.1155768 Streptococcussanguinis-6.9085379 Veillonelladispar-6.6261134 Leptotrichiashahii-4.2580925 Streptococcussanguinis-15.707148 Rothiadentocariosa-7.6363241 Gemellahaemolysans-4.0734304 Porphyromonascatoniae-2.9545948 Streptococcusinfantis-4.0082555 Capnocytophagaleadbetteri-10.916793 Leptotrichiawadei-13.784488 Neisseriaoralis-9.091896613 Prevotellaloescheii-4.29068 Fusobacteriumnucleatum-6.300239 Caenispirillumdeserti-6.593526 Capnocytophagasputigena-3.0197697 Neisseriamucosa/macacae-8.874647 Pseudopropionibacteriumpropionicum-3.2261569 Granulicatellaelegans-1.7814469 Corynebacteriummatruchotii-2.3571584 Corynebacteriummatruchotii-3.2478818 Leptotrichiashahii-2.6721703 Prevotellamelaninogenica-3.7584184 Lautropiamirabilis-5.789702414 Ottowiapentelensis-3.6497936 Gemellahaemolysans-2.0530089 Fusobacteriumnucleatum-5.8874647 Streptococcuspneumoniae/mitis/pseudopneumoniae-2.6504454 Streptococcusdentisani-4.7794916 Microlunatussoli-2.6830328 Streptococcusdentisani-0.7060613 Campylobactergracilis0-2.3462959 Rothiamucilaginosa-3.0849446 Streptococcusdentisani-2.1942212 [Clostridium]amygdalinum-2.2485336 Corynebacteriumdurum-1.140560515 Corynebacteriummatruchotii-3.2804693 Neisseriabacilliformis-1.9769715 Prevotellaloescheii-5.73539 Kingelladenitrificans-2.3897458 Veillonelladispar-4.6491419 Streptococcussanguinis-2.259396 Haemophilusparainfluenzae-0.7060613 Eikenellacorrodens-1.3578101 Streptococcusdentisani-2.2376711 Granulicatellaadiacens-1.9443841 Porphyromonaspasteri-1.7271345 Kingellaoralis -0.966760816 Campylobactergracilis0-2.5092331 Corynebacteriummatruchotii-1.1840104 Selenomonassputigena-2.5418206 Alloprevotellarava-2.2919835 Fusobacteriumnucleatum-4.3884423 Ottowiapentelensis-0.9884858 Haemophilusparaphrohaemolyticus-0.3584619 Granulicatellaadiacens-1.1188355 Campylobactergracilis0-2.009559 Haemophilusparainfluenzae-1.4881599 Lachnoanaerobaculumsaburreum-1.5967847 Actinomycesoris-0.868998517 Lautropiamirabilis-1.5750597 Streptococcusinfantis-1.0862481 Campylobactergracilis-2.1507712 Fusobacteriumnucleatum-1.737997 Leptotrichiagoodfellowii-3.117532 Leptotrichiagoodfellowii-0.9341734 Rothiamucilaginosa-0.3258744 Bergeyellazoohelcum-1.1079731 Streptococcusparasanguinis-1.716272 Gemellahaemolysans-1.3469476 Leptotrichiahofstadii-1.5641973 Actinomycesnaeslundii-0.738648718 Veillonelladispar-1.0102107 Eggerthiacatenaformis-0.6082989 Leptotrichiashahii-2.0747339 Neisseriaelongata-1.4664349 Porphyromonascatoniae-1.7488594 Actinomycesviscosus-0.836411 Chryseobacteriumcarnis-0.1955247 Lautropiamirabilis-1.0862481 Streptococcusparasanguinis-1.6728221 Capnocytophagagingivalis-1.3034977 Klebsiellapneumoniae0-1.5207473 Granulicatellaadiacens-0.315011919 Campylobactergracilis-0.9776233 Actinomycesoris-0.543124 Leptotrichiawadei-1.9986965 Neisseriaoralis-1.2274604 Capnocytophagaleadbetteri-1.6728221 Thioreductormicantisoli-0.6951988 Rothiadentocariosa-0.1629372 Porphyromonaspasteri-0.9233109 Leptotrichiahofstadii-1.3686726 Neisseriaelongata-1.3034977 Kingellaoralis -1.4121225 Eggerthiacatenaformis-0.315011920 Thioreductormicantisoli-0.9558983 Campylobactershowae-0.3801868 Prevotellamelaninogenica-1.9552466 Prevotellaloescheii-1.2274604 Porphyromonascatoniae-1.2274604 Kingellaoralis -0.6408864 Abiotrophiadefectiva-0.0651749 Gemellamorbillorum-0.8689985 Rothiadentocariosa-0.9776233 Streptococcusinfantis-0.8689985 Corynebacteriummatruchotii-1.2383228 Actinomycesnaeslundii-0.2824245Tooth4:TP3-TP2(21L:t2-t1)

species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value species_namediff_value1 Neisseriaoralis12.7634152 Porphyromonaspasteri11.3730176 Fusobacteriumnucleatum17.0106452 Alloprevotellatannerae17.3147947 Capnocytophagagingivalis7.24527482 Capnocytophagasputigena6.93026287 Streptococcussanguinis15.7940474 Lautropiamirabilis26.6022159 Streptococcuspneumoniae/mitis/pseudopneumoniae19.9543776 Streptococcusintermedius5.96350206 Lautropiamirabilis7.75581143 Streptococcuspneumoniae/mitis/pseudopneumoniae48.2076906 Streptococcuspneumoniae/mitis/pseudopneumoniae19.6393656 Leptotrichiawadei18.1512057 Streptococcussanguinis47.34955462 Streptococcusintermedius6.08298935 Fusobacteriumnucleatum7.83184879 Capnocytophagasputigena9.81968282 Veillonelladispar7.36476211 Neisseriaelongata6.3980013 Lautropiamirabilis4.60569194 Neisseriamucosa/macacae7.41907452 Rothiadentocariosa4.69259179 Gemellahaemolysans1.3143602 Neisseriamucosa/macacae5.68107756 Capnocytophagagingivalis4.8012166 Neisseriaperflava5.85487725 Capnocytophagaleadbetteri9.03758418 Veillonelladispar17.456007 Neisseriaoralis7.647186623 Ottowiapentelensis5.74625244 Gemellahaemolysans2.02042146 Paludibacterpropionicigenes6.88681295 Fusobacteriumnucleatum6.44145123 Lautropiamirabilis5.99608951 Streptococcussanguinis3.68238106 Neisseriabacilliformis6.7456007 Pseudopropionibacteriumpropionicum2.98718227 Streptococcusdentisani0.858136 Neisseriaperflava4.54051705 Streptococcuspneumoniae/mitis/pseudopneumoniae4.69259179 Streptococcusinfantis4.1168803 Leptotrichiashahii3.00890723 Corynebacteriummatruchotii6.72387573 Veillonelladispar5.985227034 Corynebacteriummatruchotii3.62806865 Neisseriabacilliformis1.97697154 Streptococcussanguinis5.09450358 Caenispirillumdeserti5.92005214 Streptococcussanguinis5.68107756 Neisseriamucosa/macacae3.39995655 Fusobacteriumnucleatum4.95329133 Campylobactergracilis02.13990876 Haemophilusparainfluenzae0.43449924 Porphyromonaspasteri3.63893113 Corynebacteriummatruchotii3.7692809 Rothiamucilaginosa3.0849446 Streptococcusdentisani2.11818379 Streptococcuspneumoniae/mitis/pseudopneumoniae5.45296546 Lautropiamirabilis2.976319795 Capnocytophagasputigena3.15011949 Capnocytophagasputigena1.73799696 [Eubacterium]yurii2.8568325 Prevotellaloescheii5.59417771 Streptococcuspneumoniae/mitis/pseudopneumoniae5.53986531 Kingelladenitrificans2.60699544 Veillonelladispar3.81273083 Kingellaoralis1.35781012 Haemophilusparaphrohaemolyticus0.16293721 Streptococcussanguinis3.45426896 Porphyromonascatoniae2.96545731 Streptococcusdentisani2.38974582 Granulicatellaadiacens1.74885944 Prevotellamelaninogenica3.7692809 Corynebacteriumdurum1.260047796 Lautropiamirabilis2.56354551 Capnocytophagagingivalis1.23832283 Corynebacteriummatruchotii2.50923311 Selenomonassputigena2.54182055 Eggerthiacatenaformis3.61720617 Neisseriaelongata1.60764719 Leptotrichiagoodfellowii3.17184445 Neisseriamucosa/macacae1.10797306 Sphingobiumamiense0.03258744 Rothiamucilaginosa2.90028242 Campylobactergracilis02.88941994 Granulicatellaadiacens2.18335868 Gemellahaemolysans1.29263524 [Clostridium]amygdalinum2.43319574 Kingellaoralis1.238322837 Leptotrichiashahii1.61850967 Corynebacteriummatruchotii1.18401043 [Clostridium]xylanolyticum2.04214643 Klebsiellapneumoniae02.43319574 Capnocytophagagingivalis2.56354551 Eggerthiacatenaformis1.40126005 Porphyromonascatoniae1.79230936 Leptotrichiagoodfellowii0.94503585 Haemophilusaegyptius0.01086248 Streptococcuscristatus2.6287204 Neisseriaelongata2.59613296 Leptotrichiahofstadii1.86834673 Gemellamorbillorum1.20573539 Lachnoanaerobaculumsaburreum1.58592222 Actinomycesoris0.814686078 Porphyromonascatoniae1.27091028 Neisseriamucosa/macacae1.05366066 Capnocytophagaleadbetteri1.72713448 Selenomonassputigena1.93352162 Streptococcusdentisani1.90093417 Porphyromonascatoniae1.12969802 Capnocytophagaleadbetteri1.52074734 Thioreductormicantisoli0.87986096 Haemophilusinfluenzae0.01086248 Capnocytophagagingivalis2.1724962 Bergeyellazoohelcum1.78144688 Streptococcusparasanguinis1.70540952 Streptococcusinfantis0.83641104 Porphyromonaspasteri1.56419726 Granulicatellaadiacens0.640886389 Streptococcussanguinis1.27091028 Granulicatellaadiacens0.6843363 Neisseriabacilliformis1.41212253 Campylobactergracilis1.91179665 Corynebacteriummatruchotii1.78144688 Leptotrichiashahii1.10797306 Leptotrichiashahii1.3143602 Bergeyellazoohelcum0.82554856 Solobacteriummoorei0.01086248 Fusobacteriumnucleatum1.29263524 Eikenellacorrodens1.50988486 Campylobactergracilis01.66195959 Porphyromonaspasteri0.73864871 Kingellaoralis1.53160982 Neisseriamucosa/macacae0.5322615710 Thioreductormicantisoli1.24918531 Eggerthiacatenaformis0.64088638 Campylobactergracilis1.28177276 Leptotrichiashahii1.81403433 Capnocytophagaleadbetteri1.73799696 Porphyromonaspasteri1.0862481 Porphyromonascatoniae1.27091028 Microlunatussoli0.82554856 Neisseriamucosa/macacae0 Aggregatibacteraphrophilus0.6843363 Fusobacteriumnucleatum1.0862481 Streptococcusparasanguinis1.64023463 Capnocytophagagranulosa0.72778623 Porphyromonaspasteri1.4881599 Actinomycesnaeslundii0.456224211 Porphyromonascatoniae-9.4612209 Streptococcusdentisani-15.348686 Leptotrichiatrevisanii-17.825331 Lautropiamirabilis-11.981317 Fusobacteriumnucleatum-12.155116 Fusobacteriumnucleatum-4.3667174 Streptococcuspneumoniae/mitis/pseudopneumoniae-38.65957 Streptococcuspneumoniae/mitis/pseudopneumoniae-38.105583 Rothiadentocariosa-4.4536172 Rothiadentocariosa-18.43363 Streptococcussanguinis-17.629807 Lautropiamirabilis-48.566153 Lautropiamirabilis-9.4720834 Klebsiellapneumoniae0-39.941343 Streptococcuspneumoniae/mitis/pseudopneumoniae-46.36106912 Capnocytophagaleadbetteri-5.3552031 Streptococcusgordonii-8.1794482 Porphyromonascatoniae-11.090593 Streptococcuspneumoniae/mitis/pseudopneumoniae-10.058657 Leptotrichiatrevisanii-4.9967413 Leptotrichiashahii-3.4216815 Kingellaoralis -9.0267217 Fusobacteriumnucleatum-2.2050836 Campylobactergracilis-2.0855964 Streptococcuspneumoniae/mitis/pseudopneumoniae-12.882902 Neisseriamucosa/macacae-16.85857 Neisseriaelongata-5.9635021 Fusobacteriumnucleatum-8.2228981 Prevotellaloescheii-16.304584 Campylobactergracilis0-5.746252413 Streptococcuspneumoniae/mitis/pseudopneumoniae-4.312405 Streptococcuspneumoniae/mitis/pseudopneumoniae-4.268955 Leptotrichiashahii-6.4197263 Fusobacteriumnucleatum-7.1909624 Neisseriamucosa/macacae-4.7360417 Alloprevotellarava-2.9980448 Campylobactergracilis0-3.3999566 Streptococcusinfantis-1.9661091 Kingellaoralis -1.7923094 Streptococcusdentisani-2.5092331 Rothiadentocariosa-3.0089072 Kingelladenitrificans-4.3015425 Neisseriaelongata-4.5513795 Leptotrichiatrevisanii-8.6573973 Gemellahaemolysans-2.932869914 Gemellamorbillorum-3.6606561 Streptococcussanguinis-2.1181838 Eggerthiacatenaformis-3.7910059 Streptococcussanguinis-5.8005648 Prevotellapleuritidis-4.3449924 Campylobactergracilis0-2.6504454 Streptococcusgordonii-1.3469476 Streptococcusdentisani-1.716272 Chryseobacteriumcarnis-1.7814469 Lautropiamirabilis-1.9335216 Neisseriaperflava-2.8568325 Capnocytophagasputigena-3.8779057 Porphyromonascatoniae-3.1718444 Fusobacteriumnucleatum-4.7143167 Porphyromonaspasteri-2.129046315 Prevotellaloescheii-3.4868564 Veillonelladispar-1.6728221 Caenispirillumdeserti-2.8785575 Eggerthiacatenaformis-5.7788399 Paludibacterpropionicigenes-3.975668 Prevotelladenticola5-1.8683467 Tessaracoccusdefluvii-1.0536607 Capnocytophagasputigena-1.4555725 Rothiamucilaginosa-1.716272 Kingellaoralis -0.9341734 Granulicatellaadiacens-1.4772974 Streptococcussanguinis-3.0740821 [Eubacterium]yurii-2.5418206 Leptotrichiagoodfellowii-1.5207473 Alloprevotellarava-1.575059716 Porphyromonasendodontalis-2.867695 Actinomycesviscosus-0.5322616 Tannerellaforsythia-2.2919835 Streptococcusdentisani-4.7034543 Caenispirillumdeserti-3.1827069 Prevotellaloescheii-1.6402346 Streptococcusdentisani-0.7277862 Granulicatellaadiacens-1.1188355 Fusobacteriumnucleatum-1.422985 Gemellahaemolysans-0.4562242 Streptococcusinfantis-1.0753856 Corynebacteriummatruchotii-2.6178579 Capnocytophagagingivalis-2.009559 Campylobactergracilis-1.3578101 Granulicatellaelegans-1.249185317 Eggerthiacatenaformis-2.6721703 Haemophilusparainfluenzae-0.4996741 Veillonelladispar-2.0204215 Porphyromonaspasteri-2.2702585 Campylobactergracilis0-2.3354334 Porphyromonascatoniae-1.44471 Neisseriaelongata-0.5322616 Streptococcussanguinis-0.8689985 Lautropiamirabilis-1.2600478 Gemellamorbillorum6-0.3801868 Rothiamucilaginosa-0.7495112 Leptotrichiashahii-1.9009342 Eikenellacorrodens-1.987834 Selenomonasinfelix-0.8907234 Porphyromonaspasteri-1.227460418 Porphyromonaspasteri-2.3788833 Actinomycesoris-0.4670867 Porphyromonascatoniae-1.9009342 Porphyromonascatoniae-1.9986965 Veillonelladispar-1.737997 Magnetovibrioblakemorei-1.3034977 Bergeyellazoohelcum-0.4453617 Corynebacteriumdurum-0.836411 Streptococcusinfantis-1.0862481 Bergeyellazoohelcum-0.3801868 Streptococcusdentisani-0.6191614 Neisseriaoralis-1.6510971 Bergeyellazoohelcum-1.987834 Porphyromonascatoniae-0.7820986 Fusobacteriumnucleatum-0.988485819 Porphyromonascatoniae-2.1724962 Corynebacteriumdurum-0.3910493 Clavibactermichiganensis-1.8357593 Neisseriaelongata-1.8900717 Fusobacteriumnucleatum-1.3686726 Neisseriaoralis-1.2383228 Leptotrichiahongkongensis-0.3584619 Capnocytophagahaemolytica-0.4236368 Capnocytophagaochracea-0.8146861 Streptococcusinfantis-0.1955247 Streptococcusvestibularis-0.3367369 Capnocytophagagingivalis-1.3903976 Neisseriaoralis-1.8357593 Parvimonasmicra-0.6843363 Streptococcusinfantis-0.934173420 Klebsiellapneumoniae0-1.5750597 Actinomycesodontolyticus-0.2281121 Altererythrobactermarinus-1.5098849 Prevotellamelaninogenica-1.7814469 Capnocytophagasputigena-1.3469476 Gemellahaemolysans-1.2057354 Rothiadentocariosa-0.2498371 Leptotrichiashahii-0.3584619 Actinomycesnaeslundii-0.6408864 Alloprevotellarava-0.1737997 Actinomycesnaeslundii-0.1737997 Fusobacteriumnucleatum-1.0210732 Leptotrichiashahii-1.5533348 Actinobaculumschaalii1-0.5974365 Neisseriaperflava-0.9233109

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