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Gramnega)ve single species bacteria Quorum Sensing Low Density High Density Biofilm A closer look inside the cells 1. Diffuses through cell membranes 2. A=aches to and ac?vates regulatory protein 3. Binds to DNA 4. Crea?on of new AHL 5. Process repeats Cell Acyl Homoserine Lactose autoinducer Ac?vator protein Chromosome AHL synthase

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Gram-­‐nega)ve  single  species  bacteria  Quorum  Sensing    

Low  Density   High  Density     Biofilm  

A  closer  look  inside  the  cells  1.  Diffuses  through  cell  membranes    

2.  A=aches  to  and  ac?vates  regulatory  protein    

3.  Binds  to  DNA    

4.  Crea?on  of  new  AHL      

5.  Process  repeats  

-­‐  Cell  

-­‐Acyl  Homoserine  Lactose  autoinducer    

-­‐  Ac?vator  protein  

-­‐  Chromosome  

-­‐  AHL  synthase