current status and future challenges in mastitis research
DESCRIPTION
A couple of years ago, we gathered with 9 individual mastitis experts, to freely discuss issues around mastitis. One of the results was an overview paper on the current status and future challenges of mastitis research. It was presented at the 50th anniversary Annual Meeting of the NMC (www.nmconline.org). These are the slides of this presentation, given by Joe Hogan and myself.TRANSCRIPT
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Current Status and Future Challenges in Mastitis
Research
Henk Hogeveen
Joe Hogan
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This mastitis forum
Eric Hillerton Henk Hogeveen Herman Barkema Joe Hogan Karin Persson Waller Satu Pyorala Steve Oliver Theo Lam Ynte Schukken
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment• Management
• Socio-economic Wrapping up
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Etiological agents• Countries, regions, farms
• Stage of lactation
• Seasons
• Evolve
Pathogens
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Staphylococci Staph aureus
• strain diversity
• related genotypes within herd
Pathogens
Photo: SVA, Uppsala
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• CNS - increasing interest in many countries• Multiple species and strains
• Dfferential diagnostics
Pathogens
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Streptococci• Strep agalactiae
• still concern in some countries
• genotypes between human and bovine isolates
Pathogens
Photo: SVA, Uppsala
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• Strep dysgalactiae • (environmental/contagious)
• fresh heifers
Pathogens
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• Strep uberis• housed and pastured systems
• strain differences
Pathogens
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Coliforms • severity = host response
• genetically heterogenous group• mild to severe
• acute to chronic
PathogensPhoto: SVA, Uppsala
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Pathogenesis• bacteria and host
Epidemiology & Ecology• genotype and environment
Emergence of ”new and old” pathogens
Future challenges - Pathogens
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Topics
Current status
• Pathogens
• Diagnosis• Immunology
• Therapy
• Environment
• Milking equipment
• Management
• Socio-economic Wrapping up
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Bacteriological culture
Somatic cell counts
Soluble indicators of inflammation
Diagnostics
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Sample collection
Sample handling
Laboratory techniques
Interpretation
Standardization
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Precision on antibiotic selection
Low specificity = false-positives
On Farm Diagnostics
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Detection of genes
ID organism
Identify virulence factors
Clonal variance
PCR
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Simple low-cost
Rapid on farm
RT Interpretation - Implementation
Future Challenges – Diagnostics
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Topics
Current status
• Pathogens
• Diagnosis
• Immunology• Therapy
• Environment
• Milking equipment
• Management
• Socio-economic Wrapping up
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Host Defense
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• SOL
• Breed
• Genotype
• Age
• Milk yield
• Nutrition
• Existing infection
Host defense
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Teat Canal Phagocytic Cells Soluble factors
Innate Immunity
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Macrophages/monocytes Lymphocytes Antibodies
Acquired Immunity
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Cytokines• Recruit
• Class switch
• Activate
• Up regulate/down regulate
Bridge Between
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Whole cell bacterins Subunit vaccines Adjuvants Route
Vaccination
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Vaccine Virulence factors Genotyping Environmental factors
Future Challenges – Host Defense
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment• Management
• Socio-economic Wrapping up
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Eliminate IMI Prudent use of antimicrobials Welfare of animal
Therapy
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28
Treatment should be targeted towards the causing pathogen
OIE World Organisation for Animal HealthTerrestrial Animal Health Code
GUIDELINES FOR THE RESPONSIBLE ANDPRUDENT USE OF ANTIMICROBIAL AGENTSIN VETERINARY MEDICINE
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Broad-spectrum antibiotics
Prudent? Efficacious?
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The longer, the better? Efficacy of longer treatment is available in:
• Staphylococcus aureus
• Streptococcus uberis
30
Duration of Treatment
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What is long? Very few comparative trials > 5 days may seldom be economical
Duration of Treatment
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Pharmacokinetics
Route and duration
Antimicrobial resistance
Economical benefits
Alternatives to antibiotics
32
Future challenges - treatment
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment• Management
• Socio-economic Wrapping up
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Biological
Physical
Chemical
Environment
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Fecal contamination
Moisture
Organic matter
Reduce Exposure to Pathogens
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Exposure = Density
Confinement Pasture
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Exposure vs Susceptibility
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Future Challenges – Environment
$ $$$$$ Waste/Recycle Ecology/Image Welfare
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment
• Milking equipment• Management
• Socio-economic Wrapping up
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Acts as a vector to transfer pathogens
Damages the first line of udder defence• Teat
• Canal
Role of machine milking in udder health
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Cyclic and irregular vacuum fluctuations
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Claw volume increased to 116 ml -9%
Small milk tube bore increased to 11 mm -16%
Add smt air bleeds (0.57 mm) -18%
Long pulse tube restrictor (3 mm) -19%
Change to alternate pulsation -20%
COMBINED EFFECT -84%
Key changes in machine milking affecting cyclic fluctuations
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Knowledge on irregular vacuum fluctuations
Blocked air bleeds Low reserve Excessive losses Ineffective regulator Liner slip Cluster attachment Cluster removal
ALL AFFECT VACUUM STABILITY AND BACTERIAL TRANSFER
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Milk flow
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Milk flow
Bad
Good
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0
20
40
60
80
0 1 2 3 4 5
Liner age
% q
ua
rte
rs
-10
0
10
20
30
40
50
60
70
0 1 2 3 4 5
Liner age
% t
eats
wit
h c
olo
ur
resp
on
se
Completeness of milking
Effect on teat color
Liner surface 0 milkings 4000 milkings
Effect of liner age
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h
2 min 5 min0 min
0 min 5 min2 min
Teat condition and overmilking
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Heifers Cows
Flow rate (ml/min) 200 400 200 400
Machine-on time (min) 5.5 5.0 7.9 7.4
Yield (kg) 21.1 21.2 31.5 31.6
Teat score 1.3 1.0 1.7 1.1
New infections, % cows 16 15 15 15From MD Rasmussen
Effect of ACR setting
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Peri-harvest diagnosis
Precision dairy farming Milk harvesting technology
• Parlor performance
• In-line/on-line detection of udder inflammation
• Other diseases
5 robots: 2 prep, 2 attach, 1 post-spray
http://www.youtube.com/watch?v=--e_AOeOC4s
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In-line/on-line diagnostics
Small amount of milk is immediately diverted into diagnostic device:• Milk meters: milk weight, conductivity, temperature.
• Diagnostic systems:
• Light scattering (fat, protein, lactose, SCC)
• Bio-sensors (LDH, progesteron, BHBA
• Electronic tongue
• Thermal camera
• Light diffusion (color)
• Capilary activity (CMT-like, SCC)
• ……
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Future challenges – machine milking
Target• Swift and complete removal of milk
• Remain the integrity of milk and health of teat Integrate sensors
• Without disrupting milking process
• Properly evaluated functioning (also economic)
• Associated “new” management Further automation Managing the teat canal
• Teat closure
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment
• Management• Socio-economic
Wrapping up
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• Dry cow antibiotics
• Post milking teat disinfection
• Milking and milking machine
• Clinical mastitis cases
• Chronically infected cows
Basis for numerous on farm udder health programs
Have shown to be effective
The 5-point plan on contagious mastitis
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6,9
5,4
2,5
1,6
0
0,9
1,5 1,4
0,9
1,21,2 1,21,4
1,7
1,1
6,2 6,1
3,4
3,7
1,8
0
1
2
3
4
5
6
7
8
1973 1975 1980 1985 2003
Years
Pre
vale
nc
e (
%)
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Staphylococcus aureus
Sampimon et al., 2009
0
1
2
3
4
5
6
7
8
1973 1975 1980 1985 2003
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Staphylococcus aureus
Dutch prevalence studies
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establishment of goals for udder health
maintenance of clean, comfortable environment proper milking procedures proper maintenance and use of milking equipment good record keeping appropriate management of clinical mastitis during
lactation effective dry cow management maintenance of biosecurity for contagious pathogens and
culling of chronically infected cows regular monitoring of udder health status periodic review of mastitis control program
The 10 point plan
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Koegebonden
Omgeving
SAG SAU SDY ECOSUB
STC
Koegebonden
Omgeving
Koegebonden
Omgeving
SAG SAU SDY ECOSUB
STC
Contagious mastitis
Environmental mastitis
Infectious pressure
Zadoks, 2002
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0%
10%
20%
30%
40%
50%
60%
70%
0 2 4 6 8 10 12 14 16 18
% nieuwe attenties
% s
core
3 e
n 4
uier
% s
core
3 a
nd
4
% s
core
3 a
nd
4
% new infectionsBMSCC
Hygiene and udder health
Schreiner and Ruegg, 2003
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Prevent other contact with the milking machine
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More than taking measures
Evaluation• Udder health
• BMSCC
• Incidence rate clinical mastitis
• Cows with high SCC
• …..
• Intermediate factors
• Udder hygiene
• Teat condition
• ….
• Tools are available
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Teat scoring strategy
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-Straightforward management:
replace after 2500 milkings
- What are farmers doing?
Remember the liners …
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0
10
20
30
40
50
3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Frequency liner replacement
Olde Riekerink and Sampimon, 2008
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05
10
15
20
Aa
nta
l bed
rijv
en
0 2500 5000 10000Tepelvoeringen worden vervangen na melkingen
Num
ber
of h
erds
70%
Milkings before replacement
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Future challenges – Management
(Further) develop management measures and tools• Applicable for a variety of modern dairy
farmers
• Straightforward
• Use of technology (precision dairy farming)
Estimate effect of management measures (“clinical trials”)• Give advisor arguments to “sell” improved
management
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment• Management
• Socio-economics Wrapping up
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Literature (€)1€ = 1.27$US
Bar2008
Halasa2009
Hagnestam-Nielsen2009
Huijps2008
Level Cow Cow Cow-year Cow
Milk production losses - 11 78 36
Labour - 11 - 4
Treatment - 14 - 15
Culling - 46 - 22
Death - 0 - 0
Veterinarian - 2 - 1
Milk quality - 0 - 0
Materials - 0 - 0
Diagnostics - 0 - 0
Total 61 84 97 78
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Mastitis costs money
Estimations: $US 77 – 123 /cow/year Variation between farms:
• The Netherlands: $US 99 -195
• US: $US 45 – 620
Farmers attitude:• Know mastitis = $$$$
• Underestimate the costs
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Specific studies on treatmentspecifically subclinical mastitis
Streptococci: € 12/treatment (Swinkels et al., 2005a)
• Partial budget model R = 0.21 Staphylococci: - € 21/treatment (Swinkels et al., 2005b)
• Partial budget model R = 0.32 Streptococci: - € 11/treatment (Steeneveld et al., 2007)
• Stochastic model Gram positive: € 2,601/100 cows
(Van den Borne et al., 2010)
• Stochastic dynamic model
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Additional expenditures
Reduced losses Net benefit
Milk cows with clinical mastitis last 37 16 -21
Milk cows with subclinical mastitis last 104 20 -84
Use of separate cloths during udder preparation 26 9 -17
Wash dirty udders during udder preparation 3 9 6
Prestripping 34 9 -25
Use of milkers’ gloves during milking 1 9 8
Post milking teat disinfection 31 31 -0
Back-flushing clusters after milking a cow with clinical mastitis 1 11 10
Back-flushing clusters after milking a cow with subclinical mastitis 123 15 -108
Replace teat cup liners in time 13 11 -2
Use of a treatment protocol 7 15 8
Application of blanket dry cow therapy 9 36 27
Keep cows standing after milking 2 12 10
Feed additional dry cow minerals 13 13 0
Prevent overcrowding 23 13 -10
Clean boxes 54 15 -39
Clean yards 51 8 -43
Optimize feed ration 24 13 -11
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Money is not the only motivator
Theory of planned behaviour…..and there are lots of other frameworks
There is an intention-behaviour discrepancy among farmers
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Future challenges – socio economics
Cost calculations at chain level• More than accumulation of individual farms
• What are economic consequences of a loss of image
Cost-effectiveness of management• Lack of clinical trials
• Effect of measure depends on assumptions (dynamics)
More insight in motivating farmers• Bringing the message
• Money
• Other factors
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Topics
Current status• Pathogens• Diagnosis• Immunology• Therapy• Environment• Milking equipment• Management
• Socio-economics Wrapping up
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Reality in developed countries
The ‘consumer’ demand:
• A safe healthy product
• Healthy happy cows
• Environmental friendly
Government (politicians) demands:
• No risk for humans Human medicine demands:
• Decrease of antibiotics and hormones in cattle• Deal with (potential) zoonotic diseases
Business behavior results in an increase in herd size
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Consequences
Consumers “in the barn” Restrictive use of antibiotics and
hormones Change in definition of “Quality” More organic farms Control of zoonotic disease requires Changed farming systems
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So …
Mastitis was and remains:• A production (economic) disease
• A welfare burden
Mastitis is becoming:• An image issue
• A sustainability issue• (less mastitis -> less culling -> less youngstock
raising -> less greenhouse gas emissions etc.
Of increased importance
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Finally
We’ve come from far, we’ve done a lot There are many challenges ahead of us
We acknowledge the Dutch dairy industry for initiating this mastitis forum