current problems2012.igem.org/files/presentation/nymu-taipei_championship.pdf · achievements we...
TRANSCRIPT
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Current problems
Population↑ Pollution↑
1 NYMU-Taipei
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The Solution: Conquering Venus &
COOL!!
NYMU-Taipei
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But...
3
1. Venus’ atmosphere…
2. Need energy, need food!!
CO2 SO2
NYMU-Taipei
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Atmosphere Perform
Long-lasting
CO2
4
H2S SO2
How about using H2S as energy source? → Sulfide-Quinone Reductase
Lots of CO2, but no O2 →Photosynthesis →Cyanobacteria
Becoming Venusians → Endosymbiosis, INV-LLO Bba_K299812 iGEM10_Warsaw (2010-10-13)
SO2 as a toxic gas →Sulfite reductase
Practical Application →Purifying polluted air
Overcoming difficulties
How about using H2S as energy source? → Sulfide-Quinone Reductase
NYMU-Taipei
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Synechocystis PCC7942
Sulfite reductase
SO2
Sulfide quinone
reductase (sqr)
chloroplast +
S + (CH2O)n
C6H12O6
For Host Cell to
Use
Whole picture of our project: Inside of the Stem Cell
Glut
CO2
H2S
SO2
CO2
H2S
5 NYMU-Taipei
Nucleus
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Synechocystis PCC7942
How to become Venusians?
Desulfovibrio desulfuricans
Synechococcus sp. PCC 7002
6 NYMU-Taipei
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Engineered Synechocystis PCC7942
How to become Venusians?
Glut (BBa M11036 )
Invasin-listeriolysin O
(BBa_K299812)
Into the
Stem cell 7 NYMU-Taipei
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CO2
8
H2S SO2 SO2 as a toxic gas →Sulfite reductase
Sulfur Dioxide (SO2) Reducer
Solved!!
Synechocystis
PCC7942
NYMU-Taipei
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Sulfur Dioxide (SO2) Reducer: The Concept
Desulfovibrio desulfuricans Pseudomonas aeruginosa
Dsr Sulfite reductase CysI Sulfite reductase
SO2 HSO3- H2S
dissolve in water Sulfite reductase
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H2S serial dilution
and standard curve detection
When H2S exist,
DPDA solution turn
turbid.
H2S producing assay
Lavilla I,Pena-Pereira .F et al, Analytica Chimica Acta 647 (2009) 112–116
Sulfur Dioxide (SO2) Reducer: The Experiment
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WORK Sulfur Dioxide (SO2) Reducer: The Results
11 NYMU-Taipei In 500μ M K2SO4 medium
Experiment OK!! More H2S!!
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H2S SO2
Using H2S to generate energy How about using H2S as energy source? → Sulfide-Quinone Reductase
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
NYMU-Taipei
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Sulfite reductase Sulfide quinone
reductase(sqr)
chloroplast
Synechocystis PCC7942
H2S
SO2
+
e-
H2S: Novel Energy Currency
NYMU-Taipei
S + (CH2O)n
CO2
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Sulfur Version of
Photosynthesis H2S S+2H++energy
SQR
NYMU-Taipei
Using H2S to generate energy: The Concept
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2H2O O2 +4H++energy
Cyanobacteria
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DCMU challenged assay
NYMU-Taipei
Using H2S to generate energy: The Results
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sqr has
advantage
DCMU Cyanobacteria growth
Sodium Sulfide consumption in E. coli SQR grows better!
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Flow cytometer – Exact cell number Auto-fluorescence assay method (2011
Schulze )
sqr strain has
larger cell number
and stronger
fluorescence signal
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Plate Count
sqr strain
Wild type
sqr strain has more colonies
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NYMU-Taipei
Using H2S to generate energy: The Results
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Sodium Sulfide
consumption in E. coli
Sodium Sulfide leftovers
after 48 hours
2.2 times!
sqr strain consumed
most of the sodium
sulfide WORK
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H2S SO2
Apoptosis
Creating Venusians
Becoming Venusians → Endosymbiosis
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
Solved!! sqr
NYMU-Taipei
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Invasin(inv) from Yersinia pestis
HEp-2 cell
Inv+
E.coli
listeriolysin O(llo) from
Listeria monocytogenes
HeLa cell
llo+ E.coli
[1]Isberg, R.R., and Falkow, S. (1985) A single genetic locus encoded by Yersinia pseudotuberculosis permits invasion of
cultured animal cells by Escherichia coli K-12. Nature 317: 262-264. [2]Denise M. Monack,Julie A. Theriot(2001).” Actin-based motility is sufficient for bacterial membrane protrusion formation and
host cell uptake”. Cellular Microbiology (2001) 3(9), 633-647
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Endosymbiosis between Eukaryotes and
Cyanobacteria(inv-llo +)
Agapakis CM, Niederholtmeyer H, Noche RR, Lieberman TD, Megason SG, Way JC, & Silver PA (2011).
Towards a synthetic chloroplast. PloS one, 6 (4) PMID: 21533097
CHO cells J774 macrophages
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cells Invasin(inv) from Yersinia pestis
listeriolysin O(llo) from Listeria
monocytogenes
Cells:
• J774 macrophage cells line
• Induced pluripotent stem cells
SO2 H2S S CysI SQR
Our work
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J774 macrophage cell line
with cyanobacteria invasion
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Induced pluripotent stem cells(iPS cells)
with cyanobacteria invasion
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H2S SO2
Practical Applications To confirm our project
Practical Application →purifying polluted air →confirming our project practically
SO2 CO2
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Practical Experiments to confirm: The Experiment Chung Hwa Pulp Corporation
26 NYMU-Taipei
Co-culture:
Engineered bacteria+
industrial waste
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For waste gas cleaning
Water layer Gas layer
Practical Applications: The Experiment
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WORK
NYMU-Taipei
H2S producing assay
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For waste water cleaning
Water layer Gas layer
Practical Applications: The Experiment
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WORK
NYMU-Taipei
H2S producing assay
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H2S SO2
Completing whole parts!!
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
Solved!! sqr
Solved!!
INV-LLO
Synechocystis
PCC7942
Work!!
NYMU-Taipei
Integrating all factors!!
Solving all problems!!
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Let’s go to VENUS!!
30 NYMU-Taipei
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Human Practice—what have they done Brand New Idea Communicating with aliens by Arecibo code Meeting with other Taiwan iGEM teams
6/1 Meeting with NTU-Taida
8/15 Taiwan iGEMer Fest (Press Release)
Having Fun
Going to Hot Spring Valley
Visiting Taipei Astronomical Museum
Advocating our project Board Game
iGEM song
iGEM short film
Advocating iGEM & synthetic biology
7/27 Synthetic Biology Worldwide
8/26 Welcome Party for Freshmen
9/13 Exhibition of iGEM in NYMU
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Arecibo code scattering around Taipei
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6/1 Meeting with NTU-Taida
8/15 Taiwan iGEMer Fest (Press Release)
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Their E.coli
works
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Creating a whole new method to exchange and
discuss ideas within Taiwan iGEM teams for themselves
and the future 2013、2014、2015… Taiwan iGEMers
Let people join iGEM in the future easier
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Advocating 2012 NYMU
iGEM team
B o a r d G a m e i G E M s o n g
i G E M s h o r t f i l m
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Advocating iGEM &
synthetic biology
7/27 Synthetic Biology Worldwide 8/26 Welcome Party for Freshmen
9/13 Exhibition of iGEM in NYMU
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To attract more people to
What do those mean ?
To let more people to what is iGEM & synthetic biology
know
To make people iGEM &synthetic biology interested in
To let some of those people who start to realize iGEM &synthetic biology to us!! join
engage in synthetic biology field!
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Achievements We have submitted 11 parts to part registry.
We have characterized and proved that these new biobrick parts:
SQR, CysI, DsrI really work as our expectation.
We have modified BBa_K299812 (INV+LLO) and elevated our
idea to a higher level, to create venusian!
We have held a meet-up with Taiwan iGEMers, with board
games, iGEM songs, movies and coorperated with NTU-
Taida.
We have already outlined and detailed an extremely new human
practice - outer space human practice project (New human
practice approaches!!).
43
NYMU-Taipei
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We are the first iGEM team who tries to…
communicate with the aliens!!
We had lots of… FUN & FUN &FUN!!
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45 NYMU-Taipei
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Reference
P1 http://www.google.com.tw/imgres?hl=zhTW&sa=X&tbm=isch&prmd=imvns&tbnid=qOU_cLnW5mjJKM:&imgrefurl=http://www.geekosystem.com/ben-stiller avataroscars/&docid=cztpwj_qRRdhBM&imgurl=http://www.geekosystem.com/wpcontent/uploads/2010/03/benstilleravatar.jpg&w=461&h=285&ei=97tnUOmbGM7umAX8hoCIAw&zoom=1&iact=rc&dur=375&sig=109169273201630242688&page=2&tbnh=139&tbnw=197&start=18&ndsp=22&ved=1t:429,r:12,s:18,i:167&tx=144&ty=61&biw=1280&bih=642
P5
computer fixerhttp://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=h2Q2KTB_ECeQFM:&imgrefurl=http://www.techsonsite.co.uk/&docid=REnxlPXOqynqOM&imgurl=http://www.techsonsite.co.uk/wp-content/uploads/2011/03/milton-keynes-computerrepairs.png&w=359&h=268&ei=Vb1nUNaWO4XLmgWCk4HICg&zoom=1&iact=hc&vpx=977&vpy=205&dur=18&hovh=194&hovw=260&tx=197&ty=124&sig=109169273201630242688&page=1&tbnh=142&tbnw=192&start=0&ndsp=18&ved=1t:429,r:11,s:0,i:101&biw=1280&bih=642
lightbulb
http://www.google.com.tw/imgres?hl=zhTW&tbm=isch&tbnid=kfPkZWWGCXwYbM:&imgrefurl=http://blog.enn.com/%3Fp%3D481&docid=MdxonwH6otD7aM&imgurl=http://blog.enn.com/wp-content/uploads/2010/10/lightbulb.jpg&w=315&h=387&ei=gL1nULjHFMXLmAX-8YGQDQ&zoom=1&iact=rc&dur=143&sig=109169273201630242688&page=2&tbnh=142&tbnw=113&start=23&ndsp=31&ved=1t:429,r:6,s:23,i:162&tx=64&ty=89&biw=1280&bih=642
47 NYMU-Taipei
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http://www.google.com.tw/imgres?hl=zh-
TW&tbm=isch&tbnid=cgZK8mOqlDXOaM:&imgrefurl=http://xiaosinyi.pixnet.net/blog/post/33570223motivation!&docid=UrV5bFu-KDNZVM&imgurl=http://4.bp.blogspot.com/_ccEezkECpdg/TSSQQ_jaMHI/AAAAAAAADsA/H1sMe6pdUKA/s1600/motivation.jpg&w=900&h=900&ei=mb1nUPScF4_JmQXQ94CwCg&zoom=1&iact=rc&dur=1&sig=109169273201630242688&page=2&tbnh=139&tbnw=139&start=17&ndsp=24&ved=1t:429,r:19,s:17,i:186&tx=69&ty=83&biw=1280&bih=642
p7
relay race
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=98OqvAVMjU7UfM:&imgrefurl=http://thepauls.wordpress.com/2011/01/31/the-gospel-baton/&docid=2trO2aDES7n-fM&imgurl=http://thepauls.files.wordpress.com/2011/01/pass_the_baton.jpg&w=1024&h=768&ei=y71nUJTJAe_LmAWiiIDQCg&zoom=1&iact=rc&dur=137&sig=109169273201630242688&page=1&tbnh=134&tbnw=230&start=0&ndsp=16&ved=1t:429,r:3,s:0,i:76&tx=110&ty=74&biw=1280&bih=642
p8
book
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=VUrSo60jjoGlSM:&imgrefurl=http://www.armenianweekly.com/2012/04/06/the-armenians-and-the-book-exhibit-to-open-at-harvard/&docid=DNtmCdMCXvPmIM&imgurl=http://www.armenianweekly.com/wp-content/uploads/2012/04/book.jpg&w=600&h=450&ei=7L1nUO-oC6POmgWu-4GoBg&zoom=1&iact=rc&dur=279&sig=109169273201630242688&page=2&tbnh=139&tbnw=188&start=18&ndsp=24&ved=1t:429,r:5,s:18,i:144&tx=83&ty=53&biw=1280&bih=642
p9
shortcut
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=p3FTTaefkBrCuM:&imgrefurl=http://lostinexcel.blogspot.com/2012/02/all-worksheet-shortcuts-one-place.html&docid=_Zhh_o5KDcQe6M&imgurl=http://4.bp.blogspot.com/-0nKLyRIQYME/T0JGf7ScQpI/AAAAAAAAEHA/rnRE-MtXIFk/s1600/worksheet%252Bshortcut%252Bkeys.jpg&w=517&h=342&ei=EL5nUPzIB6WNmQXno4CgBg&zoom=1&iact=hc&vpx=748&vpy=173&dur=96&hovh=182&hovw=276&tx=121&ty=108&sig=109169273201630242688&page=4&tbnh=128&tbnw=193&start=69&ndsp=24&ved=1t:429,r:9,s:69,i:320&biw=1280&bih=642
p10
Arecibo code
48 NYMU-Taipei
![Page 49: Current problems2012.igem.org/files/presentation/NYMU-Taipei_Championship.pdf · Achievements We have submitted 11 parts to part registry. We have characterized and proved that these](https://reader036.vdocuments.us/reader036/viewer/2022062505/5e6e20164e7fbc4b60752556/html5/thumbnails/49.jpg)
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=udPyJF8_tSyg5M:&imgrefurl=http://pzzled.yolasite.com/and-everything-in-between.php&docid=KpEVCsQ7jEh26M&imgurl=http://pzzled.yolasite.com/resources/eg-arcibo-glyph.jpg%253Ftid_1258886625188&w=462&h=460&ei=S75nUML-GYGKmQXuwIGQAQ&zoom=1&iact=rc&dur=224&sig=109169273201630242688&page=1&tbnh=131&tbnw=132&start=0&ndsp=23&ved=1t:429,r:2,s:0,i:73&tx=94&ty=70&biw=1280&bih=642
p18
surprise
http://www.google.com.tw/imgres?hl=zh-TW&sa=X&tbm=isch&prmd=imvns&tbnid=HvNqWchw5GJZuM:&imgrefurl=http://dealcetera.com/sears-canada-72-hour-surprise-sale-october-8-10th-savings-and-discounts-up-to-50-off-searsca/&docid=7C58RkoEAUraJM&imgurl=http://dealcetera.com/wp-content/uploads/2009/10/surprise.jpg&w=267&h=400&ei=yb5nUOX-PM7UmAWq4IG4BQ&zoom=1&iact=rc&dur=250&sig=109169273201630242688&page=2&tbnh=141&tbnw=94&start=18&ndsp=26&ved=1t:429,r:7,s:18,i:151&tx=55&ty=54&biw=1280&bih=642
cat ball
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=xE69VYj-jkZ-oM:&imgrefurl=http://fineartamerica.com/featured/6891-pet-impressions-cat-with-ball-kyle-adamache.html&docid=KSw-QmydUtAoIM&imgurl=http://images.fineartamerica.com/images-medium/6891-pet-impressions-cat-with-ball-kyle-adamache.jpg&w=525&h=700&ei=6L5nUP-QNozMmAWJu4AY&zoom=1&iact=rc&dur=241&sig=109169273201630242688&page=2&tbnh=144&tbnw=117&start=18&ndsp=24&ved=1t:429,r:5,s:18,i:144&tx=73&ty=98&biw=1280&bih=642
join us
http://www.google.com.tw/imgres?start=163&hl=zh-TW&tbm=isch&tbnid=vEmiKOcs244jwM:&imgrefurl=http://km2.iiietc.ncu.edu.tw/xms/content/show.php%3Fid%3D29907&docid=y9V1AVfLMAvYFM&imgurl=http://km2.iiietc.ncu.edu.tw/xms/data/256/content/86E6DD72A5A058C0DB5D/attach/C1000086JoinUs.jpg&w=600&h=848&ei=Er9nUJuuCerKmgWSvoHYCQ&zoom=1&iact=hc&vpx=472&vpy=278&dur=1437&hovh=267&hovw=189&tx=119&ty=183&sig=109169273201630242688&page=8&tbnh=133&tbnw=94&ndsp=25&ved=1t:429,r:2,s:163,i:243&biw=1280&bih=642
49
NYMU-Taipei