confidential applications for field service engineers

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Applications for Field Service Engineers

CONFIDENTIALHPLC Applications Training

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HPLC Applications TrainingMOBILE PHASE CONSIDERATIONS

Flammability Toxicity Miscibility Viscosity Dissolved Salts %Organic Solvent pH (Acid/Alkali) Carbon Dioxide Corrosion

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HPLC Applications TrainingSome Flammable Solvents Commonly Used Acetonitrile Methanol Hexane, iso-Hexanes, Heptane, iso-Octane Tetrahydrofuran Toluene Acetone Ethyl Acetate Isopropanol

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HPLC Applications TrainingSome Toxic Solvents Commonly Used Acetonitrile Methanol Hexane Tetrahydrofuran Toluene Dichloromethane (Methylene Chloride) Chloroform Dimethyl Sulphoxide

CONFIDENTIALHPLC Applications TrainingBURDICK & JACKSON

UV Cutoff (nm)

BURDICK & JACKSON

UV Cutoff (nm)

Acetonitrile UV 190 Ethylene Dichloride 228

Water 190 Dichloromethane 233

Hexane UV 195 Chloroform 245

Cyclohexane 200 Ethyl Acetate 256

Heptane 200 Dimethyl Acetamide 268

Isopropyl Alcohol 205 DMF 268

Dimethyl Sulfoxide 268

Methanol 205 Toluene 284

Ethyl Alcohol 210

Trifluoroacetic Acid 210 1,2,4-TCB 308

Tetrahydrofuran UV 212

1,4-Dioxane 215 Acetone 330

Ethyl Ether 215 Methyl Isoamyl Ketone 330

Iso-Octane 215 Methyl Isobutyl Ketone 334

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HPLC Applications Training HPLC SOLVENTS - UV CUTOFF

Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell.

UV Cutoff (nm) Acetonitrile UV 190 Pentane 190 Water 190 Hexane UV 195 Cyclopentane 198 Cyclohexane 200 Heptane 200 Isopropyl Alcohol 205 Methanol 205 Ethyl Alcohol 210 2-Methoxyethanol 210 Methyl t-Butyl Ether 210 n-Propyl Alcohol 210 Trifluoroacetic Acid 210 Tetrahydrofuran UV 212 n-Butyl Alcohol 215 1,4-Dioxane 215 Ethyl Ether 215

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HPLC Applications Training

CONFIDENTIALHPLC Applications TrainingHPLC Columns

Silica particles (1.5~100μm)

Polymer particles (3~50µm)

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HPLC Applications Training

SAMPLE PREPARATION

Filtration – Membrane Filters 0.2µm, 0.45µm (Cellulose Acetate, PVDF)

Centrifugation – Economical, less contamination, multiple processing

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HPLC Applications TrainingPS335 Photodiode Array Detector

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Applications for Field Service Engineers

confidential applications for field service engineers

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