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CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

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Page 1: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

CHAPTER 9Proteins and Their Synthesis

CHAPTER 9Proteins and Their Synthesis

Copyright 2008 © W H Freeman and Company

Page 2: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

CHAPTER OUTLINE9.1 Protein structure

9.2 Colinearity of gene and protein

9.3 The genetic code

9.4 tRNA: the adapter

9.5 Ribosomes

9.6 The proteome

Page 3: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Chapter 9 Opener

Surface of the ribosome from the bacterium Haloarcula marismortui

Page 4: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-1

The binding of a drug molecule to the ribosome prevents translation

Page 5: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Protein structure

Page 6: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-2a

The peptide bond

Page 7: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-2b

The peptide bond

Page 8: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-3

Levels of protein structure

Page 9: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-3a

Levels of protein structure

Page 10: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-3b

Levels of protein structure

Page 11: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-3c

Levels of protein structure

Page 12: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-3d

Levels of protein structure

Page 13: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Colinearity of gene and protein

Page 14: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-4

Gene and protein sequences are colinear

Page 15: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

The genetic code

Page 16: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-5

Overlapping versus nonoverlapping genetic codes

Page 17: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-6

The genetic code

Page 18: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

tRNA: the adapter

Page 19: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-7

The structure of transfer RNA

Page 20: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-8

An aminoacyl-tRNA synthetase attaches an amino acid to its tRNA

Page 21: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-9

Two superimposed tRNAs

Page 22: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-10

Wobble allows one tRNA to recognize two codons

Page 23: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Table 9-1

Page 24: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Table 9-2

Page 25: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Ribosomes

Page 26: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-11

Protein and RNA molecules compose the two subunits of aribosome

Page 27: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-12

rRNA folds up by intramolecular base pairing

Page 28: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-13

Key sites of interaction in the ribosome

Page 29: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-13a

Key sites of interaction in the ribosome

Page 30: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-13b

Key sites of interaction in the ribosome

Page 31: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-14

Shine–Dalgarno sequence

Page 32: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-15

Translation initiation in prokaryotes

Page 33: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-15 part 1

Translation initiation in prokaryotes

Page 34: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-15 part 2

Translation initiation in prokaryotes

Page 35: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-16

Translation initiation in eukaryotes

Page 36: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-16 part 1

Translation initiation in eukaryotes

Page 37: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-16 part 2

Translation initiation in eukaryotes

Page 38: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-17

Steps in translation elongation

Page 39: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-18

Termination of translation

Page 40: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-19

A suppressor counteracts the effects of a nonsense mutation

Page 41: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

The proteome

Page 42: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-20

Alternative splicing produces related but distinct protein isoforms

Page 43: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-21

Phosphorylation and dephosphorylation of proteins

Page 44: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-22

All protein interactions in an organism compose the interactome

Page 45: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-23

Ubiquitinization targets a protein for degradation

Page 46: CHAPTER 9 Proteins and Their Synthesis CHAPTER 9 Proteins and Their Synthesis Copyright 2008 © W H Freeman and Company

Figure 9-24

Signal sequences target proteins for secretion