cellular respiration part 2 producing atp by oxidative phosphorylation energy from macromolecules
TRANSCRIPT
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Cellular RespirationCellular RespirationPart 2Part 2
Producing ATP by Oxidative Producing ATP by Oxidative PhosphorylationPhosphorylation
Energy from Macromolecules Energy from Macromolecules
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Releasing Energy From GlucoseReleasing Energy From GlucoseGlucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
CytoplasmCytoplasm
MitochondrionMitochondrion
Citric Acid Citric Acid CycleCycle
ATPATPee-- Carriers Carriers
ee-- Carriers Carriers
ee-- Carriers Carriers
Electron Transport Chain
Electron Transport Chain
½ O½ O22
HH22OO
ATPATP
2 H2 H++
4 CO4 CO22
2 CO2 CO22
ATPATP
GlycolysisGlycolysis
EnergyReleased
ee--
ee--ee--
EnergyReleased
OO2 2 presentpresent
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Locations of Cellular Respiration ComponentsLocations of Cellular Respiration Components
Matrix: Citric Acid Cycle Matrix: Citric Acid Cycle and Pyruvate Oxidationand Pyruvate Oxidation
MitochondrionMitochondrion
A CristaA CristaInnerInnerMembrane:Membrane:Has ATP Synthase Has ATP Synthase Electron Transport ChainElectron Transport Chain
IntermembraneIntermembraneCompartmentCompartment
HH++ accumulates accumulates
aabb
OuterOuterMembraneMembrane
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Sequence of Sequence of Electron CarriersElectron Carriers
The poison cyanide prevents
transfer of electrons to
oxygen
NADH donates electrons to
Complex I(NADH
Dehydrogenase)
FADH2 donates electrons to
Complex II (Succinate
Dehydrogenase)
Cytochromes are electron
carriers with a heme prosthetic
group
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Formation of Formation of HH++ Gradient Gradient
The poison arsenic prevents the buildup of the
H+ gradient
Protons are pumped from the
matrix into the intermembrane
space
Flow of protons through ATP
synthase powers ATP production
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ATP SynthaseATP Synthase
H+ ions cause the rotor of ATP
synthase to spin
Sites in the catalytic knob are activated to
catalyze ATP production
The inner mitochondrial membrane is
impermeable to H+, which can
only pass through the ATP synthase
The internal rod also spins as a result of rotor
movement
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Oxidative PhosphorylationOxidative Phosphorylation
• Production of ATP as a result of electron transfer Production of ATP as a result of electron transfer through carriers in the Electron Transport Chainthrough carriers in the Electron Transport Chain– Electrons pass through a set of membrane-associated Electrons pass through a set of membrane-associated
carriers by a series of redox reactionscarriers by a series of redox reactions– Energy from electron transport powers the active Energy from electron transport powers the active
transport of Htransport of H++ to the intermembrane compartment of the to the intermembrane compartment of the mitochondrion, building a concentration gradient mitochondrion, building a concentration gradient
– Chemiosmosis: Diffusion of hydrogen ions (HChemiosmosis: Diffusion of hydrogen ions (H++) through ) through the differentially permeable inner mitochondrial the differentially permeable inner mitochondrial membrane, resulting in ATP production membrane, resulting in ATP production
• HH+ + can only cross the membrane into the mitochondrial can only cross the membrane into the mitochondrial matrix through the pores of an ATP-synthesizing enzymematrix through the pores of an ATP-synthesizing enzyme
• Movement of HMovement of H++ through the enzyme provides energy for through the enzyme provides energy for ATP synthesisATP synthesis
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FermentationFermentation
Glucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
CytoplasmCytoplasm
MitochondrionMitochondrion
Citric Acid Citric Acid CycleCycle
ATPATPee-- Carriers Carriers
ee-- Carriers Carriers
ee-- Carriers Carriers4 CO4 CO22
2 CO2 CO22
ATPATP
GlycolysisGlycolysis
oxidized eoxidized e-- Carriers Carriers
FermentationFermentation 2 X Lactate (3C)2 X Lactate (3C)(in muscle)(in muscle)OO2 2 presentpresent
when Owhen O22 becomes available becomes availableXX OO2 2 absentabsent
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Alcoholic and Lactic Acid Alcoholic and Lactic Acid FermentationFermentation
Muscle cellsMicroorganisms
YeastsSome Plants
Alcoholic Fermentation
Lactic Acid Fermentation
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Energy From MacromoleculesEnergy From Macromolecules
Glucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
Citric AcidCitric AcidCycleCycle
GlycolysisGlycolysis
MonosaccharidesMonosaccharides
PolysaccharidesPolysaccharides
DisaccharidesDisaccharides
GluconeogenesisGluconeogenesis
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Energy From MacromoleculesEnergy From Macromolecules
Glucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
TriglyceridesTriglycerides
Fatty Acids Fatty Acids multiples of 2C multiples of 2C
GlycerolGlycerol(~5%) (~5%)
Citric AcidCitric AcidCycleCycle
Glucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
GlycolysisGlycolysisGluconeogenesisGluconeogenesis
DAPDAP
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Energy From MacromoleculesEnergy From Macromolecules
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
ProteinsProteins
Other amino Other amino AcidsAcids
3C-amino 3C-amino acidsacids
Citric AcidCitric AcidCycleCycle
Glucose (6C)Glucose (6C)
GlycolysisGlycolysisGluconeogenesisGluconeogenesis
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Anabolic InterconversionsAnabolic Interconversions
Glucose (6C)Glucose (6C)
2 X Pyruvate (3C)2 X Pyruvate (3C)
2 X Acetyl-CoA (2C)2 X Acetyl-CoA (2C)
Citric AcidCitric AcidCycleCycle
GlycolysisGlycolysis
PolysaccharidesPolysaccharides
GluconeogenesisGluconeogenesis
Fatty AcidsFatty Acids
GlycerolGlycerol
Amino AcidsAmino Acids
TriglyceridesTriglycerides
ProteinsProteins
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Regulation of Regulation of GlycolysisGlycolysis
• Phosphofructokinase is Phosphofructokinase is – allosterically inhibited allosterically inhibited
by ATPby ATP– allosterically activatedallosterically activated
by ADP or AMPby ADP or AMP– inhibited by citrateinhibited by citrate
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Regulation of the Citric Acid CycleRegulation of the Citric Acid Cycle
• Isocitrate dehydrogenaseIsocitrate dehydrogenase– responds to negative responds to negative
feedback from NADHfeedback from NADHand H+ and ATPand H+ and ATP
– is activated by ADP and is activated by ADP and NAD+NAD+
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Regulation of Regulation of Acetyl-CoAAcetyl-CoA
• Entering the Citric Acid Entering the Citric Acid Cycle Cycle – Citrate synthase (1)Citrate synthase (1)
is inhibited by ATP is inhibited by ATP or NADHor NADH
• Use in Fatty Acid Use in Fatty Acid SynthesisSynthesis– Fatty Acid synthase (2)Fatty Acid synthase (2)
is stimulated by Citrateis stimulated by Citrate
(1)
(2)