cell-based therapy of myelin disease under imaging guidance piotr walczak, m.d. russell h. morgan...
TRANSCRIPT
Cell-Based Therapy of Myelin Disease Under Imaging Guidance
Piotr Walczak, M.D.Piotr Walczak, M.D.
Russell H. Morgan Department of Radiology and Radiological Science and Russell H. Morgan Department of Radiology and Radiological Science and Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins
University, Baltimore, MD, United States University, Baltimore, MD, United States
Cell Therapy Approach in Myelin Disease
Normal Myelin Myelin DiseaseTransplantation of Stem/Progenitor Cells New Myelin
Normal Myelin (MBP) Dysfunctional Myelin (MBP)
Transplanted Human Oligodendrocyte Precursors(HuNu)
New Myelination by Human Cells (HuNu, MBP)
The Role of Imaging in Developing Cell-Based Therapy of Myelin Disease
• Therapeutic effect depends on:survival, migration, appropriate differentiation, and functional integrationof transplanted cells within the host
• New myelination by grafted cells works well in young, developing recipients but is more challenging in the adult, disease damaged host
• Non-Invasive monitoring is critical for optimization of cell therapy
We monitor survival and differentiation of grafted cells with bioluminescent imaging
We monitor cell delivery, migration and compact myelin
formation with MRI
We monitor axonal conduction with electrophysiology
T2* T2, DTI