Carol Thompson

Carol ThompsonProcessing Manager

NHSBT

Carol Thompson

To give an overview of the processing of blood products and the different processes involved before it leaves NHSBTDescribe the different products produced

Carol Thompson

Carol Thompson

Aim to make the most from each donationTurn a single donation into:

Red cellsPlasma productPlatelets

Carol Thompson

Approximately 22 component donations and 800 whole blood donations each day.7 days a weekReceive in the afternoon or evening of day 0 and then again at 06:45 on day 1

Carol Thompson

Can be made into single, double or triple product.Split depending on product volumeAim for equal amounts in each packEnd up with approx 40 to 60 separate products

Carol Thompson

All products are QM to ensure process is in control and products meet specificationCheck for

Platelet countWhite cell countVolumeHctProtein levelsFVIII level

Carol Thompson

Minimum 36 hours after donationAll apheresis and pooled platelets are tested

Carol Thompson

Sampled for Aerobic and Anaerobic organismsIncubated for life of products

Carol Thompson

Platelets labelled and stored in platelet agitatorMandatory testNegative result necessary for release to Validation

Carol Thompson

Aim to maximise the use of each donationUsed to make FFP, cryoprecipitate and red blood cellsProcess the majority of the blood on day 1

Carol Thompson

Ambient overnight hold of whole bloodSorted into pack types, male and female, time bled and productStrict timelines

Carol Thompson

Whole blood mixedHung up on filtration rackFiltered to remove white cellsEmpty collection pack and filter cut off

Carol Thompson

Packed upCentrifugedSeparation of red cells and plasma

Carol Thompson

Processed on optipressPlasma removed into separate transfer packSAGM added to red cellsFFP has air removed then frozen

Carol Thompson

Frozen plasma removed from freezerPlaced on trolleys in fridgeDefrosted slowly overnight

Carol Thompson

The cryo precipitates out of the plasmaCentrifugedRemoval of waste plasmaRe-frozen

Carol Thompson

Single cryo defrosted rapidlyHeated and mixed5 singles used to make 1 pool

Carol Thompson

Carol Thompson

Products presented to PulseChecks that all mandatory testing is completedLabel produced

Carol Thompson

Red cells stored in walk in fridges at 4 degree

Phenotype stockExchange donationsLVT s

Platelets in RT incubator

HLA matched

Frozen products in walk in freezer

Carol Thompson

Other products made on request PAS plateletsWashed red cells

Carol Thompson

Orders sent in from the hospital via OBOSStaff issue the products to that hospital completing the audit trail for that donation.

Carol Thompson