Can we collect data yet? time Can we collect data yet? No. time Can we collect data yet? No. Yes. time Can we collect data yet? No. Yes, but no. Yes. time First light! June 8, 2001 ALS BL 8.3.1 First light! June 8, 2001 ALS BL 8.3.1 First light! June 8, 2001 ALS BL 8.3.1 Problems? Decoupling detector from sample Beam stability Does the beamline know were trying to collect? Time of day Flux out of mono (nA) Exercise wavelength (3 motors) Time of day Flux out of mono (nA) Exercise wavelength (3 motors) Beam off Pinhole Time of day Flux out of mono (nA) Exercise Theta (1 motor) Time of day Flux out of mono (nA) Exercise Z2 (1 motor) Time of day Flux out of mono (nA) Shutter stability Time (s) Flux (nA) Shutter stability before after Detector comparison procedure RunIringExposeFramesXtal AXtal B Detector comparison procedure beamline diff detectorQ210Q315125% SN Distance(mm)150 - Wavelength (A) Divergence (h)1.5 - Divergence (v) ~16.7% Flux (ph/s)1.1 x10 10 ~0 Detector comparison results beamline diff % frames multiplicity~100 - R merge (%)6.2(10.7)6.4(10.3)-3.1% I/sig(I)90.6(66.3)85.2(63.9)6.3% Ranom (%)4.1(4.7)4.1(4.5)0.0% Detector comparison results beamline diff MADPPH % MPADPPH % FOM(SAD) % FOM(DM) % Rcryst (wARP) % Rfree (wARP) % CC (model) % CC (8.3.1 map)