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California Association of Criminalists 135 th Fall Seminar 2021 October 18-22, 2021 Hilton Santa Cruz in Scotts Valley Hosted by CA DOJ Bureau of Forensic Services Jan Bashinski DNA and Central Coast Laboratories

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California Association of Criminalists135th Fall Seminar 2021

October 18-22, 2021Hilton Santa Cruz in Scotts Valley

Hosted byCA DOJ Bureau of Forensic Services

Jan Bashinski DNA and Central Coast Laboratories

Table of Contents

Welcome Note………………………………………………….1Week at a Glance……………………………………………… 2Hotel Map……………………………………………………... 3Monday Workshop Descriptions……………………………….4Tuesday Workshop Descriptions……………………………….5Wednesday General Session Schedule & Notes………………. 6Thursday General Session Schedule & Notes………………… 8Friday General Session Schedule & Notes……………………. 10General Session Abstracts……………………………………...12Poster Session Abstracts………………………………………. 20Exhibitor Acknowledgements………………………………….22Special Thanks………………………………………………… 24

Welcome to Santa Cruz! Enjoy your week!

Page 1

Welcome From the CAC President

Thank you all for attending the 135th Seminar of the California Association of Criminalists! On behalf of the host committee and the Board, we welcome you to beautiful Santa Cruz County! There are so many things to enjoy: from the Santa Cruz Boardwalk, to taking the steam train at the Roaring Camp Railroads, to visiting the many beaches and scenic West Cliff Drive!

This seminar was originally scheduled for April 2020, but it was postponed due to the current COVID-19 pandemic. With the current safety protocols in place, we aim for this seminar to be successful, educational, and fun; but still keeping our members and our community safe. Our host committee and host laboratories have done a wonderful job to make this a fantastic seminar! If you see any of the following host seminar committee members, please thank them for all their hard work:

Host Seminar Committee MembersMary Hong (co-chair)Meghan Mannion Gray (co-chair)Jaime BaxterMegan CaulderDarlene EspejoEric HalsingVivien LeeGunther ScharnhorstLinh SchulzChristopher TanforanJeanette Wallin

We are so excited that you are attending. We look forward to the many presentations and workshops being presented at this seminar. Have a great and fun week! Please wear your mask, stay safe, and be well!

Best Wishes,

Catherine CurrierPresident, California Association of Criminalists

Week at a Glance

MondayOctober 18

9:00 AM - 5:00 PMDNA Workshop

Forest II/III_____________

9:00 AM - 12:00 PMSuccessful Interviewing Workshop

Forest I_____________

1:00 PM - 4:50 PMIntroduction to Glass & Paint Examination(Trace) Workshop

Sequoia_____________

8:00 PM - 11:00 pmHospitality Suite

Room # TBD

TuesdayOctober 19

8:00 AM - 5:00 PMHuman ID Workshop

Forest II/III_____________

8:00 AM - 11:45 AMCourtroom

Testimony WorkshopForest I

_____________

12:45 PM - 5:00 PMLeeds Workshop

Sequoia_____________

8:00 PM - 11:00 pmHospitality Suite

Room # TBD_____________

5:30 PMCAC Board Meeting

Executive Board Room

WednesdayOctober 20

8:00 AM - 8:30 AMOpening remarks

and Exhibitor IntroductionsForest II/III

_____________

8:30 AM - 4:15 PMGeneral Session

Forest II/III_____________

4:15 PM - 5:30 PMBusiness Meeting

Forest II/III_____________

6:00 PM - 8:00 PMWine & Cheese

Reception/New Member

Reception/Poster Session

Veranda_____________

8:00 PM - 11:00 pmHospitality Suite

Room # TBD

ThursdayOctober 21

8:00 AM - 5:00 PMGeneral Session

Forest II/III_____________

5:30 PM - 10:00 PMCAC Banquet and Awards Ceremony Cocoanut Grove, Beach Boardwalk

FridayOctober 22

8:00 AM - 12:00 PMGeneral Session

Forest II/III_____________

ABC DNA Exam:Registration:

10:00 AMExam:

10:30 AM - 12:45 PMSequoia

ABC Comprehensive Criminalistics &

Foundational Knowledge Field

Testing Exam:Registration:

1:15 PM - 1:45 PMEXAM:

1:45 PM - 4:45 PMForest I

Lunch Information:Monday & Tuesday: VerandaWednesday & Thursday: Stonehouse Grill/Bar

Breaks:Monday & Tuesday (AM and PM): VerandaWednesday & Thursday (AM and PM): Forest IFriday morning break: Veranda

Page 2

Wine & Cheese Reception / New Member Reception / Poster Session:Wednesday evening, 6:00 PM – 8:00 PM: Veranda

Banquet and Awards Ceremony:Thursday evening, 5:30 PM – 10:00 PM: Cocoanut Grove, Santa Cruz Beach Boardwalk

Transportation will be provided.

Hotel/Meeting Rooms Map

Page 3

Stairwells to get to 1st

floor bathrooms

Rear Elevators to get to 4th floor for additional bathrooms

Private Dining Room located on 1st floor

Mon

day,

10-1

8-20

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DNA Workshop:Various Instructors and Presenters

This is a full day workshop intended to satisfy the Federal Quality Assurance continuing education requirement. The presentations will include a variety of topics including current and future technologies as well as case presentations.

Read all of the abstracts for the DNA Workshop by opening your smartphone’s camera and pointing it at this QR code.

Successful Interviewing Workshop:Raymond Davis (CourtSkills) and John Houde (Calico Press)

Most candidates perceive the interview process as a necessary but unsolvable mystery. It’s painful to watch bright, talented and well qualified candidates fail because of the nature of the interviewing process. The reason for this situation lies with universities who fail to provide interview training for their graduates. Their attitude is that a degree from their institution is sufficient to win the position. Coincidentally, there is also a real loss for the organization if the candidates they interview fail because of the uncertainty of the interviewing process. Both sides of the table have a vested interest in creating a positive interview environment along with well trained, capable interviewees. I would propose that any laboratory holding interviews conduct a workshop a few weeks in advance of the interview process to minimize errors from both sides of the table.

Although there are many self-help books on interviewing, most fail to provide the best information for earning the position. I have spent the past 25 years gathering information and interviewing over 250 candidates to arrive at the best advice for people who are just beginning their career and for those who wish to advance their career. This workshop will cover the all the important aspects of the interviewing process which will improve the candidates chances of winning the position.

Raymond will share with attendees five critical questions most candidate fail to answer successfully: The self-introduction question, the Strength/Weakness question, the Ethics question, Where you see yourself in five years question and Why should we hire you, question. Attendees will have the opportunity to practice answering theses question receiving feedback from the instructor and other students.

Introduction to Glass & Paint Examination (Trace) Workshop:Trevor Gillis MS, ABC-GKE; Melissa Dupée, Criminalist – Trace Evidence UnitCrime Laboratory, County of Santa Clara, Office of the District Attorney

This workshop will provide a general introduction to the analysis of glass, paint, and polymer evidence. Designed for practitioners who don't have a glass or paint program, but may encounter glass, paint, or polymer evidence in casework. Attendees are expected to have basic polarized light microscopy knowledge.

Page 4

Workshops

WorkshopsTuesday, 10-19-2021

Human ID Workshop: Various Instructors and Presenters

This is a full day workshop featuring presentations on different methods for identifying unidentified human remains and missing persons.

Read all of the abstracts for the Human ID Workshop by opening your smartphone’s camera and pointing it at this QR code.

Courtroom Testimony: Raymond Davis (CourtSkills) and John Houde (Calico Press)

Over the past 30 years, many of my students from the Courtroom Presentation of Evidence course have asked for one thing: More confidence. They want to be able to walk into the courtroom brimming with confidence with no trace of nervousness. Rarely did any of these students ask to be smarter or more talented or more capable. Technical skills were seldom an issue with them. Their overriding concern was to be the best witness possible in conveying their opinions in a strong, confident voice. This is not an easy assignment given the rigors of our adversarial system of justice. Raymond plans to present the best modules from his 3 day course stressing the elements that will increase the expert’s confidence while minimizing the nervousness associated with public speaking. Most experts are uncomfortable being in the spotlight (the witness stand), preferring the anonymity and the comfort of the laboratory environment. Our anxiety begins to ramp up when we receive the subpoena to testify and doesn’t end until we’re asked to step down from the witness stand. Raymond will share with you his strategy for successfully navigating this judicial process in order to look forward to testifying. Providing a hint of what to expect at this workshop, Raymond will discuss the one question that will establish your confidence early setting the tone for the remainder of your time on the witness stand. Imagine how comfortable you’ll feel when you see lawyers and jurors alike smiling in appreciation of your expert presentation. Students will be asked to participate and get real time feedback from Raymond as well as from the other students.

Automated Comparison of 3D Renderings for Bullets and Cartridge Cases, AutoIdentification functions, and Virtual Comparison Microscopy (VCM) – Evofinder:Neal Schrode, Evofinder Product Manager, Leeds

3D images of bullets and cartridges can be used for creating a database of evidence samples that allows for rapid, automated comparison between samples throughout the entire database. This technology also allows for pre-screening samples prior to examination on the microscope. This year, Leeds will be hosting a workshop to discuss and demonstrate how 3D imaging systems can scan, store, compare, and display sample representations for comparison of bullets and cartridge cases to expedite the examination workflow of samples in the laboratory, and provide the ability to match samples from different crimes, allowing for the association of previously un-related crimes to aid in the judicial system. This workshop will involve a presentation on the Evofinder systems technology, how the system works on deformed bullets, and will contain a hands-on portion for scanning bullets and ammunition into the Evofinder system to show how the system makes correlations and can provide for direct sample comparison. The Workshop will include a Q&A session as well as an opportunity to provide feedback. Please note that the first half of this workshop will be instructional, with the second half as a hands-on use of the Evofinder®. (We encourage you to bring your own samples for use with the hands-on portion of the workshop.)

Page 5

Wed

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1Morning Moderator: Jaime Baxter, Senior Criminalist, BFS Jan Bashinski DNA Laboratory

8:00 am – 8:15 am Welcome and Opening Remarks Barry Miller, Director, Bureau of Forensic Services

8:15 am – 8:30 am Vendor Introductions Jaime Baxter, Senior Criminalist, Department of Justice

8:30 am – 10:15 am Keynote Speakers: “The Jayme Closs Abduction” Jeffrey Nelson, Detective, Barron County Sheriff’s Department Joe Welsch, Special Agent, Wisconsin DOJ Division of Criminal Investigation

10:15 am – 10:35 am Morning Break*** Sponsored by NicheVision Forensics ***

10:35 am – 12:30 pm “The Jayme Closs Abduction” (continued)

12:30 pm – 1:30 pm Lunch Break

Afternoon Moderator: Meghan Mannion Gray, Criminalist Supervisor, BFS Jan Bashinski DNA Laboratory

1:30 pm – 2:15 pm “Familial Search Case- Antunez and Dwyer”Clinton Cole, Senior Deputy, San Luis Obispo County Sheriff’s Office

2:15 pm – 2:45 pm “Marijuana Enforcement: A Whole New World… Kind Of”Michael McKinney, Assistant District Attorney, Santa Cruz County Ed Browne, Assistant District Attorney, Santa Cruz County

2:45 pm – 3:15 pm “Distinguishing Hemp from Marijuana Quickly, Easily, and Accurately Using a Novel MidInfrared Spectrometer” Dr. Brian C. Smith, CEO/CTO, Big Sur Scientific

3:15 pm – 3:45 pm Afternoon Break

3:45 pm – 4:15 pm “Too Close to the Action: Avoiding Conflicts of Interest in the Laboratory”Michael Chamberlain, Deputy Attorney General, CA Department of Justice

4:15 pm – 5:30 pm CAC Business Meeting

6:00 pm – 8:00 pm Wine and Cheese Reception/New Member Reception/Poster Session*** Sponsored by QIAGEN ***

8:00 pm – 11:00 pm Hospitality Suite*** Sponsored by Thermo Fisher Scientific ***

Page 6

General Session

Wednesday, 10-20-2021

Page 7

NOTES

General SessionTh

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1Morning Moderator: Brian Harmon, Criminalist Manager, BFS Jan Bashinski DNA Laboratory

8:00 am – 9:00 am Founders Lecture: "A Woman...A Private Forensic Science Laboratory...A Journey“Carol Hunter, Retired Laboratory Director, CA Laboratory of Forensic Science

9:00 am – 9:30 am “The Argument That Lasted 39 Years -- The Case Study of the Teresa Broudreaux Murder”Jamie Lajoie, Senior Criminalist, Los Angeles Sheriff's Department, SSB

9:30 am – 10:10 am “One Scarf Too Many”Alice Hilker, Supervising Criminalist, San Mateo County Sheriff's Office LabMatt Broad, Inspector, San Mateo County District Attorney's Office

10:10 am – 10:30 am Morning Break *** Sponsored by Verogen ***

10:30 am – 11:00 am “OSAC's Role in Forensic Science Standards and What It Means for You”Mark Stolorow, Contractor, NIST/OSAC

11:00 am – 12:00 pm “Burning, Wildfires, and Human Remains Recovery”Dr. Alison Galloway, Emerita Professor, University of California at Santa Cruz

12:00 pm – 1:00 pm Lunch Break

Afternoon Moderator: Mary Hong, Criminalist Manager, BFS Freedom & Santa Barbara Laboratories

1:00 pm – 1:45 pm “Mass Fatality Identification: A Look at Identifying Victims in the Camp Fire and Conception Dive Boat Fire”Kimberly Gin, Coroner, Sacramento County

1:45 pm – 2:30 pm “Fentanyl Tablets, Vaping Cartridges, and a New THC Analysis Methodology” Dr. Charmaine Qiu, Senior Forensic Chemist, DEA Western Laboratory

2:30 pm – 2:45 pm “A Killer Victim” Rachel Frase, Senior Criminalist, BFS Freedom Laboratory

2:45 pm – 3:15 pm Afternoon Break

3:15 pm – 3:35 pm “Not Your Typical Date Rape Evidence: A Forensic Video Case Study”Cordelia Willis, Criminalist, Santa Clara County District Attorney’s Crime Lab

3:35 pm – 4:20 pm “TeleSART: Virtual Response to Sexual Assault”Dr. Sheree Goldman, Nurse Practitioner/SANE-A/SANE-P, Monterey SARTLana Nassoura, Deputy District Attorney, Monterey County DA’s Office

4:20 pm –4:40 pm “Whole Genome Sequencing of Rootless Hair for Forensic Genetic Genealogy”Dr. Kelly Harkins Kincaid, Founding CEO, Claret Bioscience and Astrea Forensics

4:40 pm –5:00 pm “There’s a Hole in my Barrel! A Case Study”Jessica Winn, Criminalist Supervisor, BFS Fresno Laboratory

5:30 pm – 10:00 pm Banquet and Awards Ceremony at the Cocoanut Grove*** Sponsored by Calico Press ***

Page 8

Thursday, 10-21-2021

Page 9

NOTES

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2021

Moderator: Vivien Lee, Criminalist Supervisor, BFS Jan Bashinski DNA Laboratory

8:00 am – 8:05 am Spring 2022 Seminar AnnouncementJamie Lajoie, Senior Criminalist, Los Angeles Sheriff's Department, Scientific Services Bureau

8:05 am – 9:05 am “The Christina Williams Case: Justice Served After 22 Years”Matthew L’Heureux, Deputy District Attorney, Monterey CountyLinh Schulz, Senior Criminalist, BFS Freedom LaboratoryEric Halsing, Senior Criminalist, BFS Jan Bashinski DNA Laboratory

9:05 am – 9:20 am “Interview or Interrogation?”Raymond Davis, President, CourtSkills

9:20 am – 10:05 am “When Forensics Don’t Fit”Kenton Wong, Senior Forensic Scientist, Forensic Analytical Crime Lab, Inc.

10:05 am – 10:25 am Morning Break*** Sponsored by Raymond Davis - CourtSkills ***

10:25 am – 11:05 am “Luck favors the prepared, darling."Corey Schroeder, Latent Print Analyst II, BFS Fresno Laboratory

11:05 am – 11:30 am “Elephants, Ivory, and Trafficking: How the CDFW Forensics Laboratory isHelping to Combat the Illegal Ivory Trade”Kelly Carrothers, Senior Wildlife Forensic Specialist, California Department of Fish and Wildlife, Law Enforcement Division

11:30 am – 12:00 pm “Details of an Officer Involved Shooting: From the Laboratory to the Courtroom”Michael Appel, Criminalist Supervisor, BFS Sacramento Laboratory

Page 10

General Session

10:00 am – 12:45 am ABC DNA ExamSequoia Room

1:15 pm – 4:45 pm ABC Comprehensive Criminalistics & Foundational Knowledge Field Testing ExamForest I

NOTES Friday, 10-22-2021

Page 11

General Session AbstractsWEDNESDAY:

“The Jayme Closs Abduction”Jeffrey Nelson, Detective, Barron County Sheriff’s DepartmentJoe Welsch, Special Agent, Wisconsin Department of Justice- Division of Criminal Investigation

The presentation involves the October 15th, 2018 investigation of the abduction of Jayme Closs and the homicides of her parents. It will cover how local, state and federal agencies worked together and co-managed the investigation until Jayme’s heroic escape on January 10th, 2019. It also covers the successful prosecution of Jake Patterson and difficulties encountered during the process.

“Familial Search Case- Antunez and Dwyer”Clinton Cole, Senior Deputy, San Luis Obispo County Sheriff’s Office

I will be presenting on the use of a familial DOJ search to solve two 40-year-old homicides from 1977 and 1978.

“Marijuana Enforcement: A Whole New World … Kind of”Michael McKinney, Assistant District Attorney, Santa Cruz County Ed Browne, Assistant District Attorney, Santa Cruz County

This presentation will discuss the impact that the legalization of recreational marijuana has had on criminal prosecution of illegal marijuana grows, as well as alternate avenues to enforce the new regulations. It will talk about new areas of focus for criminal investigations, as well as civil and administrative proceedings to abate illegal marijuana grows and enforce the regulations and restrictions on growing and selling marijuana.

“Distinguishing Hemp from Marijuana Quickly, Easily, and Accurately Using a Novel Mid-Infrared Spectrometer”Dr. Brian C. Smith, CEO/CTO, Big Sur Scientific

In the last few years legal hemp has been seized around the country, and innocent people have been jailed despite the legality of hemp that contains 0.3% Total THC or less. Also, forensic labs and state departments of agriculture are being swamped with samples whose legality needs to be determined, and chromatography is too slow and expensive to handle the workload. There is a need then for law enforcement to distinguish between hemp and marijuana in a fast, easy, accurate, and portable fashion.

We have developed a rugged, novel, portable, mid-infrared spectrometer that successfully distinguishes hemp from marijuana over 99% of the time. The unit requires little sample preparation, features push-button operation so anyone can use it, and there are no consumables so the cost per analysis is $0. The system is already in use by law enforcement agencies in the USA. How the system works and its calibration and validation will be discussed.

“Too Close to the Action: Avoiding Conflicts of Interest in the Laboratory”Michael Chamberlain, Deputy Attorney General, CA Department of Justice

You are asked to perform forensic casework, but quickly realize that the crime was committed in your neighborhood. What should you do? How about a case where you know the victim, or have a personal relationship with an investigator or witness? What if you realize that your private-sector lab is working a case for the prosecution, but did work for the same defendant in a prior case? This presentation will discuss various conflicts of interest that a crime lab or its employees may face. It will survey relevant legal principles, consider ways to prevent conflicts, and offer responsive strategies when conflicts are unavoidable. This presentation will satisfy CAC's ethics training component.

Page 12

General Session AbstractsTHURSDAY:

“A Woman...A Private Forensic Science Laboratory...A Journey”Carol Hunter, Retired Laboratory Director, California Laboratory of Forensic Science

The lecture will begin with a historical overview of female pioneers in Forensic Science during the 1930-40’s, and women entering a male dominated forensic science workplace in the 1960-70’s. There will be a brief discussion of the significant impact of LEAA funding upon the advancement of the forensic science training and origins of federally funded regional forensic laboratories in the United States in the 1970’s. Finally, an auto-biographical sketch of my education and early career choices that led me to founding a full service private forensic science laboratory in the early 1980’s. There will be an outline and overview of my philosophy toward quality, integrity and ethical standards for this private forensic laboratory. The contrast/similarities in operational structure to government laboratories, including case approach, client maintenance, and budgets. There will also be discussion of the importance of professional organization experience and giving back to the profession.

“The Argument That Lasted 39 Years -- The Case Study of the Teresa Broudreaux Murder”Jamie Lajoie, Senior Criminalist, Los Angeles Sheriff's Department, Scientific Services Bureau

On March 4, 1980 a young mother, Teresa Broudreaux, was found dead by a surfer on the sands of Malaga Cove Beach in Palos Verdes Estates, California. Teresa was found naked, bloodied, bruised and with blunt force trauma to her head. The last person to see Teresa alive was her husband, who stated she left their home the prior evening after an argument. This case study will cover the process, challenges, and rewards of working a 39 year old cold case.

“One Scarf Too Many”Alice Hilker, Criminalist Supervisor, San Mateo County Sheriff's Office Forensic LaboratoryMatt Broad, Inspector, San Mateo County District Attorney's Office

On March 4, 1989 at approximately 0443 hours, San Mateo Police Department officers responded to the victim's apartment in San Mateo on a reported rape and stabbing. They located the victim who was holding a cloth to her neck. Despite a severe laceration to her neck and stab wounds on her back, the victim described her assailant as a, “Hispanic male adult, 5-6 to 5-8, 150 to 160 lbs (‘Not in very good shape’), wearing a bandana around his head.” The victim told investigators that the suspect entered her apartment in the middle of the night and attempted to bind her with scarves. He then physically and sexually assaulted her before stabbing her and cutting her throat. The victim fought her assailant and then succeeded in creating a dialogue with him; wherein she was able to convince him to leave her alive. During the initial investigation, two males were arrested after they were selected from photographic lineups. Both males were subsequently excluded via enzyme typing. No other suspects were identified, and the case went cold. In 2005, the San Mateo County Sheriff’s Office Forensic Laboratory examined the evidence in the case. Several items of evidence were tested, one of which was a floral scarf with a stain which yielded a single source male DNA profile. The unknown DNA profile was uploaded into CODIS, but no hits were located. The profile was also sent to Cal DOJ for a familial comparison, but no potential family members were identified.

In 2020, San Mateo County DA’s Office investigators re-opened the case and re-examined the DNA. From the new examination and investigative leads, a possible suspect was identified. Background research indicated that this suspect ran a side business as a DJ. An investigator used some creative methods to obtain a surreptitious DNA sample. The findings provided the basis for an arrest warrant that was issued for the suspect who was arrested and is currently awaiting trial.

Page 13

General Session AbstractsTHURSDAY (continued):“OSAC's Role in Forensic Science Standards and What It Means for You”Mark Stolorow, Contractor, NIST/OSAC

This presentation will educate participants on the role that the NIST-administered Organization of Scientific Area Committee (OSAC) for Forensic Science plays in the development and implementation of forensic science standards and how those activities impact forensic science practitioners.

In February 2014, the United States’ National Institute of Standards and Technology (NIST) launched and began administering the Organization of Scientific Area Committees (OSAC) for Forensic Science, which is a collection of 500 volunteer members (practitioners, researchers, statisticians, legal experts, human factors experts and quality experts) divided into 20+ forensic science discipline specific subcommittees. This effort was designed to improve forensic science discipline specific quality and enhance the operational effectiveness of the U.S.’s 400+ crime laboratories. Additional targeted stakeholders include 18,000 law enforcement agencies, 2,300 medical examiner/coroner offices, 6,000 public defender offices, and 3,000 prosecutor offices which increases the challenge of generating awareness of standards and providing a uniform message across all these independent stakeholders as there is no single owner/regulator of forensic science in the U.S.

Significant progress has been made in engaging the forensic community and its stakeholders in the development of forensic discipline-specific documentary standards. To date, OSAC has placed over 50 standards on its OSAC Registry and has several hundred more still under development. This presentation will help attendees understand the role OSAC plays and how OSAC’s activities could impact your agency’s operations.

“Burning, Wildfires, and Human Remains Recovery”Dr. Alison Galloway, Emerita Professor, University of California at Santa Cruz

The increase in California wildfires has taxed the ability of the local communities to respond. Mutual aid for search and rescue/recovery has become the norm but the recovery of human remains is hindered by the lack of an accessible pool of forensic anthropologists whose work includes location, identification and recovery of human remains. This situation is changing with coordination by the California Office of Emergency Services. However, it is important for local agencies to know what services anthropologists can provide, when best to call for their assistance and what areas of work should be included.

This presentation uses the Camp Fire in Butte County to describe the recovery efforts, focusing on the anthropologists. While much of the work involved the location and excavation of remains, there was also a less visible but very critical role in the dispatch of identification services to support the Search and Rescue/Recovery teams. This combined effort produced excellent recovery of the dead.

The human remains in wildfires are usually heavily calcined/cremated. Bone fragments are mixed with drywall and other structural material and difficult to identify. Nonhuman material is also commonly present from household pets and mounted trophy skulls to food or decorative items. Already cremated remains are frequently present in homes and can produce positive responses from cadaver dogs trained on burned remains. Basic recovery methods need to maximize the collection of all human remains, general separation of the non-bone and nonhuman material and preparation for transportation with minimal damage.

For the Camp Fire, the collection work was usually handled by multi person teams of senior anthropologists and students, primarily from CSU Chico, which houses a large forensic anthropology program. Other universities also sent teams to assist. However, the scale of the devastation was so vast that these teams could not respond to all suspected finds. Smaller teams were held centrally, composed of graduate students and alums, who could be sent to any SAR group that thought they had found human remains. Only once these were confirmed and the location clearly marked were the recovery teams notified of a location.

Recovered remains were sent to the Sacramento County Coroner's facilities, which had sufficient room to handle the volume of material. Here, teams of pathologists, odontologists, anthropologists and rapid DNA analysts worked to

Page 14

General Session AbstractsTHURSDAY (continued):

sort and identify the bodies. Complications arose over incomplete recoveries, split recoveries, and commingled remains, as well as some nonhuman and non-bone inclusions. Anthropologists were critical for sorting of remains and detailing where and to what extent remains were intermingled - and whether or not they could be sorted.

Of the 85 victims of the Camp Fire, all but one has been identified. Most were identified by rapid DNA and some more by dental records. Only a few were given circumstantial identifications. The response of Butte County was commendable and serves as a model for other locations as they plan for the aftermath of a wildfire in inhabited areas.

“Mass Fatality Identification: A Look at Identifying Victims in the Camp Fire and Conception Dive Boat Fire”Kimberly Gin, Coroner, Sacramento County

In 2018 and again in 2019, two major mass fatality events occurred in the State of California. The 2018 Camp Fire in Butte County was the deadliest wildfire in the history of California. In September of 2019, the Conception Dive Boat Fire off the Coast of Santa Barbara County claimed 34 lives. In both events, Sacramento County played a major role by assisting Butte and Santa Barbara County with identifications for these victims.

In the Camp Fire, Sacramento County utilized various identification methods including Odontology, fingerprints, hardware identification and Rapid DNA. This was the first time that Rapid DNA technology had been used in this capacity in the United States and in doing so has changed the traditional way of identifying victims in these events. This was very evident in the Conception Boat Fire where all 34 victims were identified utilizing Rapid DNA.

After receiving the request from Santa Barbara County Sheriff’s Office for assistance in identification via Rapid DNA, the Sacramento County Coroner’s Office responded to Santa Barbara County with the instrument and supplies. The outcome was remarkable. All 34 victims were recovered in 10 days’ time. Those same victims were identified by Rapid DNA in the same time frame with the final victim located and also identified on Day 10. All DNA processing of human remains and familial reference samples was performed by a single operator, and DNA results were obtained the same day samples were made available for testing. This presentation outlines the identification process and subsequent outcomes for both events.

“Fentanyl Tablets, Vaping Cartridges, and a New THC Analysis Methodology”Dr. Charmaine Qiu, Senior Forensic Chemist, DEA Western Laboratory

In recent years, the DEA Western Laboratory has seen an increase in various clandestine tablets submissions. This presentation will discuss the “Mexican Oxycodone” tablets, found to contain fentanyl and/or fentanyl related substances. DEA Western Laboratory has also analyzed various “vape pens” and other vaping cartridges recently. These vape cartridges contained a variety of controlled substances.

Our DEA laboratories have newly implemented a new methodology to analyze cannabis plant material. The new analytical scheme and procedures are utilized to distinguish between potential hemp and marijuana as defined in the Agricultural Improvement Act of 2018, H.R. 2, 115th Cong. (2018). A Decision Limit (DL) value for total ∆9-tetrahydrocannabinol (THC) present at 1% is established for laboratory reporting purposes.

“A Killer Victim”Rachel Frase, Senior Criminalist, BFS Freedom Laboratory

A case study of the local California DOJ crime lab’s contribution to the investigation of the 2018 death of a Great White Shark from the Monterey Bay, after two bullets were discovered in the shark’s skull.

Page 15

General Session AbstractsTHURSDAY (continued):

“Not Your Typical Date Rape Evidence: A Forensic Video Case Study”Cordelia Willis, Criminalist, Santa Clara County District Attorney’s Crime Laboratory

Most Date Rape trials focus on DNA or toxicology evidence, but this 2011 case—which was tried in 2017 and again in 2019—centered on the forensic video evidence. This talk will demonstrate how watching the participants’ actions in the hour and a half leading up to the incident made a difference to the triers of fact.

“TeleSART: Virtual Response to Sexual Assault”Dr. Sheree Goldman, DNP and SART Coordinator, Monterey County Health DepartmentLana Nassoura, Deputy District Attorney, Monterey County Office of the District Attorney

Attendees will learn about the development and implementation of a temporary protocol during the shelter in place order in 2020. Survivors of sexual assault in Monterey County were offered an option to remain sheltered in place while being interviewed and guided through evidence collection. The presentation will include a short video depiction of the process, DOJ lab analysis of the evidence, and admissibility of such evidence in court.

“Whole Genome Sequencing of Rootless Hair for Forensic Genetic Genealogy”Dr. Kelly Harkins Kincaid, Founding CEO, Claret Bioscience and Astrea Forensics

This presentation demonstrates the value of rootless hair for use in DNA analyses, including investigative genetic genealogy. Rootless hair and other highly degraded samples like bone are dominated by ‘ultrashort’ DNA fragments, e.g. 30-60 bp in length. These fragments are present in minute quantities and are often overwhelmed by the presence of exogenous, non-human DNA, e.g. microbial DNA. For these reasons, forensic samples, especially from cold cases, greatly benefit from the use of ancient DNA (aDNA)-based methods. The methods for DNA extraction and whole-genome sequencing (WGS) library preparation are designed to retain the shortest DNA fragments possible while maximizing the number of unique DNA molecules available for sequencing. Using an example success story of Snohomish County’s “Precious Jane Doe”, we show that by applying aDNA-based techniques to samples such as a single rootless hair it becomes possible to capture and sequence short, degraded fragments of DNA from both the nuclear and mitochondrial genome. The resulting low-coverage WGS data can be combined with an imputation approach to generate genotype files suitable for genetic genealogy investigation, in this case, leading to the positive identification of Lisa Roberts 43 years later.

“There’s a Hole in my Barrel! A Case Study”Jessica Winn, Criminalist Supervisor, BFS Fresno Laboratory

A suspect’s pistol was submitted to the laboratory as part of an officer involved shooting. When submitting the suspect’s pistol, the agency was concerned as to whether comparisons could be performed as “the gun had been hit by an officer’s round,” but no other information was given on the pistol. Skeptical as to the extent of the damage, the agency was assured that comparisons may still be performed even with damaged firearms.

Once submitted, however, the extent of the damage to the suspect’s pistol was shocking and never before seen. Determining the possible source of the damage proved to be challenging, but interesting as well. This case study presentation will discuss the extent of the damage to the pistol, the probable cause of the damage, and resulting "shooting reconstruction." The eventual comparison of the inoperable firearm to cartridge cases from the scene will also be discussed.

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General Session AbstractsFRIDAY:

“The Christina Williams Case: Justice Served After 22 Years”Matthew L’Heureux, Deputy District Attorney, Monterey County Office of the District AttorneyLinh Schulz, Senior Criminalist, BFS Freedom LaboratoryEric Halsing, Senior Criminalist, BFS Jan Bashinski DNA Laboratory

This was a cold case that started on June 12, 1998, involving a missing 13-year-old female who walked her dog one evening and never returned home. Her remains were found 7 months later. A lot of evidence was submitted to the FBI lab for analysis with no probative results. In 2016, this cold case was re-opened and evidence was submitted to the DOJ Freedom and Richmond labs for analysis. The presence of one sperm head ultimately led to the conviction of Charles Holifield, a convicted felon who was charged with kidnapping and sexually assaulting teenage girls and was already serving a 25 years to life sentence.

“Interview or Interrogation?”Raymond Davis, President, CourtSkills

For over 25 years, I have had the privilege of interviewing candidates for positions in the crime laboratory. I have met many capable, smart, and well-qualified candidates who failed to win the position for a variety of reasons for which this presentation will focus on.

Most universities fail to provide any training for their graduates believing that simply displaying their diploma to the hiring manager will automatically gain them the position. That happens to be the attitude of one major university in Pennsylvania that prevented one of its own professors the opportunity to teach interviewing skills training at their business school. Another, closer example, Boise State University did not want me to continue training their soon to be graduates believing that it wasn’t necessary. I could tell the students were eager to learn more but the professors denied their request telling them it was a waste of time.

I have seen first-hand the folly of this attitude as hundreds of candidates trooped into the interview room unaware of what was expected of them. From their attire (some looked like they were ready to start a career at Burger King!), to fumbling of simple questions, to forgetting the panelists names and worse, what the position entailed. Most knew very little about the laboratory and not one person could name the lab director! Some, inexplicably made snide remarks during their tour of the facility angering the staff. One candidate was so loathed by the staff that they told us they would all quit if we hired him! Ouch.

One major concern I have for our profession is that we must not miss the opportunity to hire the very best candidates possible. Our profession and our organization thrive when we find and hire talented people. When I worked at the DOJ – DNA in Point Richmond, I mentioned my concern to the panelists who encouraged me to act on my recommendations. I was worried that we were losing great potential employees due to nervousness and lack of knowledge about the interviewing process. To improve the candidate’s performance, I would meet with them 30 minutes prior to their interview. I greeted them with a smile and handed them the first portion of the interview for their perusal – The Interpersonal Interview Questions. They had the opportunity to consider the questions and decide on their best responses. I saw a dramatic improvement in each person’s performance as they seemed more confident and less nervous. I’m convinced that some of these candidates did much better than they expected because of this strategy.

Further, I told my fellow panelists to be more engaging with each candidate requiring us to write less and paying more attention to them. No one likes answering questions to someone who’s not paying any attention. On occasion, we would interject some appropriate humor that often helped many candidates to relax resulting in a better performance. In some cases, the candidate would offer some humor in return that helped everyone to relax. My recommendation is twofold. First, invite all the candidates to the laboratory for a ‘meet and greet’. Introduce the candidates to the panelists over coffee and donuts which will take about an hour or so of staff time. During that meeting, give the candidates an idea of what’s expected of them. From their attire and grooming to the structure of the interview, showing them the various ways in answering a question. When the day arrives and the candidate finally

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General Session AbstractsFRIDAY (continued):

walks into the interviewing room, they won’t be meeting strangers. Plus, they will be prepared to give their best interview answers which can only be beneficial to the laboratory’s decision-making process.

Second, provide some questions in advance of their interview that will prepare the candidate to build their confidence and calm their nerves. Doesn’t the same thing happen for us when we give our qualifications prior to our expert testimony? Why not do the same thing for them. You will see a dramatic improvement which can only make each laboratory’s effort to find new members more efficient. One other thing I would recommend, is that when we saw a candidate struggling for an answer, we offered them the opportunity to return to the question when they were ready. You could hear an audible sigh of relief! I don’t recall anyone passing on that suggestion.

Interviewing should not feel like an interrogation. The more knowledgeable and relaxed the candidate - the better their effort to impress upon you why you should hire them. If after all the effort you’ve made to ensure the best conditions possible for the candidate and they still fail, you’ve lost nothing. However, the effort you make to ensure the best environment for them will guarantee that you will not reject a great potential employee.

“When Forensics Don’t Fit”Presenter: Kenton Wong, Senior Forensic Scientist, Forensic Analytical Crime Lab, Inc.

In January 2013, 94yo Leola Shreves was brutally murdered within the bedroom of her residence. Curiously, $4000 was not taken from the scene despite being in plain sight. Law enforcement focused on the next door neighbor, 20yo Michael Alexander as the primary suspect. While the application of forensic science to a case reconstruction can assist to reveal the identity of a suspect and potential details of a crime incident, in this particular case study, none of the forensics fit the evidence of the theory of how the case transpired, yet despite this, Michael Alexander remained in-custody for over three years before finally being released. After over six years since Leola Shreves was murdered, a full DNA profile matching the numerous bloodstains recovered from the crime scene hit in CODIS to a suspect named Armando Arias Cuadras, which resulted in his arrest for the murder of Leola Shreves. This case is a sobering reminder that as forensic scientists, we need to trust the science and remain objective in the scientific findings.

“Luck Favors the Prepared, Darling.” Case Study of the Jaime Harmer Homicide and Dismemberment InvestigationCorey Schroeder, Latent Print Analyst II, BFS Fresno Laboratory

A case study: Riverside Police Department homicide investigation from April 27, 2014. Victim was dismembered and placed into 10 plastic bags. Latent print processing and analysis of the submitted evidence developed a usable latent print impression that was identified to the suspect. This and additional forensic evidence lead to a conviction.

“Elephants, Ivory, and Trafficking: How the CDFW Forensics Laboratory is Helping to Combat the Illegal Ivory Trade”Kelly Carrothers, Senior Wildlife Forensic Specialist, California Department of Fish and Wildlife, Law Enforcement Division

Illegal poaching and wildlife trafficking is the fourth largest transcontinental crime, worth an estimated $20 billion each year. In an effort to curb ivory trafficking in California, a law was enacted in 2015 (AB 96, codified as Fish and Game Code section 2022) that prohibits the purchase, sale, offer for sale, possession with intent to sell, or importation with intent to sell of ivory from elephant, mammoth, and mastodon as well as other non-proboscidean species. To support efforts in prosecuting wildlife crimes, the California Department of Fish and Wildlife’s Wildlife Forensics Laboratory has developed and validated a multiplex High-Resolution Melt (M-HRM) assay for the

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General Session AbstractsFRIDAY (continued):

identification of proboscidean taxa commonly required to be identified or excluded in ivory seizures and forensic casework: Asian elephant (Elephas maximus), African elephant (Loxodonta spp.), mammoth (Mammuthus spp.), and mastodon (Mammut spp.). Five hundred and fifty (550) blood, tissue, and ivory samples from individuals of these 4 proboscidean taxa were used to develop and validate the 2 proboscidean-specific mitochondrial sites targeted by this assay. The 28-basepair (bp) 16S ribosomal RNA (rRNA) and 54-bp cytochrome b (Cytb) gene segments yield a combination of melt peaks that create composite melt profiles unique to each of the 4 proboscidean taxa. Furthermore, the composite melt profiles of African elephants appear to provide insight into geographic provenance of the ivory- an observation that is further confirmed through mitochondrial control region haplotyping and microsatellite genotyping. Ongoing investigation into the geographic assignment of African elephant ivory to its source country using the composite melt profiles obtained through the M-HRM technique will increase the utility of this assay in law enforcement situations. Once complete, wildlife forensic laboratories and enforcement officials can use this sensitive, rapid, and cost-effective assay to assist efforts to combat the unlawful commercialization of proboscidean ivory and to stop the poaching crisis at its source.

“Details of an Officer Involved Shooting: From the Laboratory to the Courtroom”Michael Appel, Criminalist Supervisor, BFS Sacramento Laboratory

October 31, 2016, A Fresno County Deputy Sheriff was fatally shot while in the office among other deputies. Eyewitness accounts of the incident were contradictory and the investigation revolved around the incident being an accidental discharge by the victim or an accidental discharge by another deputy. The ‘other’ deputy in question was later charged by the Fresno County District Attorney’s Office for involuntary manslaughter. Several requests were made to perform various forensic examinations involving the firearm in question, which included firearm drop testing, comparison, cartridge case ejection pattern analysis, muzzle-to-target distance determination, and holster retention testing. Subsequent to forensic examination, the firearm examiner was called to testify to the results on a preliminary hearing and later a jury trial hearing. This presentation will discuss the case from the incident to the forensic examination to the courtroom presentation of evidence, and ultimately the court’s conclusion.

“American Academy Standards Board (ASB) Firearms and Toolmarks Consensus Body- 2021 Update”

Author: Gregory Laskowski, President/Chief Consultant, Criminalistics Services International, LLC

Status of New Work Proposals:• Standard 163 (024-FireTool-NWP: Standard Test Method for the Restoration of Obliterated Serial Numbers and

Other Markings on Metal – CB approved this NWP on January 13, 2021. ASB Ballot to approve this NWP closed and passed on January 28, 2021

• Best Practice Recommendation 060: Guidelines for Barrel and Overall Length Measurements for Firearms –Public comment and CB ballot close on June 14, 2021. Chair: Jennifer Floyd

• Standard 061: Firearms and Toolmarks 3D Measurement Systems and Measurement Quality Control – nearing/in the publication process Chair: Ryan Lilien

• Standard 062: Standard for Topography Comparison Software for Toolmark Analysis – nearing/in the publication process Chair: Ryan Lilien

• Standard 063: Implementation of 3D Technologies in Forensic Firearm and Toolmark Comparison Laboratories –nearing/in the publication process. Chair: Ryan Lilien

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• Standard 096: Standard Method for the Examination and Documentation of Ammunition and Ammunition Components – CB to approve resolutions of public comments. Chair: Jennifer Floyd

• Standard 100: Range of Conclusions and Criteria in Toolmark Examinations – PINS published on April 5, 2019. ASB has document. WG has scheduled meetings. Chair: Jennifer Floyd

• Standard 101: Supporting Documentation of Source Conclusions in Toolmark Examinations – PINS published on April 5, 2019. ASB to receive document and CB to form WG. Note: This NWP may be withdrawn, upon CB approval. Chair: TBD

• Standard 102: Standard for Peer Review of Source Conclusions in Toolmark Examinations – Revised PINS published on January 24, 2020. WG has scheduled meetings. Chair: Jennifer Floyd

• Best Practice Recommendation 103: Guidelines for Reporting of Source Conclusions in Toolmark Examinations –PINS published on April 5, 2019. ASB to receive document and CB to form WG. Chair: TBD

• Standard 104: Standard for 3D Virtual Comparison Microscopy for Firearm and Toolmark Analysis –PINS published on August 10, 2018. ASB to receive document and CB to form WG. Chair TBD

• Standard 105: Minimum Education Requirements for Firearm and Toolmark Examiner Trainees – nearing/in the publication process. Chair: Jennifer Floyd

• Best Practice Recommendation 107: Best Practice Recommendation for Measuring Trigger Pull of a Firearm and Estimating Its Uncertainty – CB to approve resolutions. Chair: Jennifer Floyd

• Standard 124: Minimum Requirements and Recommendations for a Firearm and Toolmark Examiner Training Program – PINS published on June 14, 2019. ASB to receive document and CB to form WG. Chair: TBD

• Standard 162 (023-FireTool-NWP): Standard for the Forensic Examination and Documentation of Non-Firearm Tools and Toolmarks – PINS was published by ANSI on May 28, 2021.

• Standard 164 (025-FireTool-NWP): Standard Test Method for Muzzle to Target Distance Determinations – PINS was published by ANSI on May 28, 2021.

The Best Practice Recommendations and Standards published by the American National Standards Institute (ANSI) are listed below:

Published:1. Best Practice Recommendation 068: Safe Handling of Firearms and Ammunition – September 4, 2020.2. Standard 093: Test Method for the Examination and Testing of Firearms – November 27, 2020

Poster Session Abstracts“A Comparison of the Sensitivities and Specificities of Common Latent Blood Detection Reagents, BluestarForensic Magnum and Weber’s Luminol Formula”Presenter: Kylie Edwards, University of California at Santa Cruz

Two common latent blood detection reagents, Weber’s Luminol Formulation and Bluestar Magnum, are compared based on their sensitivities, effect on the Kastle Meyer presumptive test for blood, and selectivity for blood and potential production of false positives when in contact with different cleaning reagents and substrates. From the multi-phased comparison of the two latent blood detection reagents, it may be concluded that Luminol is a more advantageous latent blood detection reagent. Luminol was experimentally determined to produce positive chemiluminescent reactions that were consistently detected by the naked eye up to 1:3,200 and up to 1:6,400 as detected by the SLR camera with a 30 second shutter speed. These dilutions are compared to Bluestar, where positive chemiluminescent reactions were consistently detected by the naked eye up to 1:1,600, and up to 1:3,200 as detected by the SLR camera. The application of Luminol also decreases the Kastle Meyer reagents detection limit by one dilution

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Poster Session Abstractsfactor on average, compared to Bluestar’s two dilution factor decrease on the KM detection limit post-application. Lastly, there are seemingly less false positives obtained through the application of Luminol, giving an experimental specificity of 0.08, which is compared to Bluestar’s experimental specificity of 0.16. This further leads to a possible conclusion that Luminol has a heightened sensitivity and specificity for blood. Although the dilution factors producing positive chemiluminescent reactions varied throughout the multi-phased comparison, it is still determined that Luminolis potentially a more advantageous latent blood detection reagent to use at crime scenes due to its greater sensitivity, lesser effect on the Kastle Meyer, and its greater specificity for blood due to its lesser potential to produce false positives.

“Next Generation Sequencing using Probe Capture: De-convolution of mixtures with mitochondrial DNA, STR, and SNP markers from a single shotgun DNA library”Authors: Gunmeet Kaur Bali*, Mary Wisner, Shelly Shih, Sandy Calloway, and Henry Erlich, UCSF Children's Hospital Oakland Research Institute (*presenter)

The clonal property of Next Generation Sequencing (NGS), also known as Massively Parallel Sequencing facilitates the de-convolution of mixtures, a common and challenging category of crime scene evidence. Using a probe capture strategy for target enrichment enables the analysis of the short and degraded DNA fragments common in many forensic specimens since the alternative strategy of PCR enrichment requires the presence of two intact priming sites. Moreover, a single shotgun library prepared from a forensic sample can, in principle, be analyzed with probe panels for mitochondrial DNA (mtDNA), Single Nucleotide Polymorphism (SNPs), and Short Tandem Repeats (STRs), maximizing the genetic information that can be extracted from quality and quantity compromised forensic samples.We have developed NimbleGen probe panels for the entire mtDNA genome (Shih. et al. 2018), for 496 SNPs (Bose et al. 2018), and for 44 STR genetic markers. The captured libraries are sequenced on the Illumina MiSeq platform. Sequencing the entire mitochondrial DNA genome, a haploid lineage marker allows de-convolution of mixtures by counting individual clonal sequence reads and assigning them to one of the contributors, aided by a phylogenetic based software, Mixemt (Vohr et al. 2017). This software can estimate the number of contributors as well as their proportions in the mixture; this information can be helpful in interpreting the SNP and STR analyses of the same mixtures. We have recently reported the analysis of several contrived forensic type mixtures (e.g., hair + blood) using our mtDNA probe panel and Mixemt (Wisner et al, 2020). Recently, we have developed a probe panel for 44 different STR loci which includes 30 autosomal, 5 X STRs, 8 Y STRs and amelogenin marker. The probes target unique flanking sequences, and the sequence files are analyzed with the web-based software toaSTR (Ganschow et al. 2018) developed by LABCON-OWL, for genotyping NGS STRs. The toaSTR analysis of STR markers enables the identification of STR alleles that are identical by size but different by sequence and can distinguish biological (iso-)alleles from stutter and other artifacts which is useful in mixture interpretation. The SNP analysis is valuable in highly degraded forensic samples where the STR analyses may be compromised. Our custom SNP probe panel consists of a set of 496 nuclear SNPs (367 SNPs and 129 microhaplotype markers). The presence of tri-allelic, tetra-allelic, as well as the microhaplotypes markers in our SNP probe panel facilitates possible mixture detection and analysis. Our experience with contrived mixtures indicates that counting sequence reads for mtDNA markers can be used to estimate proportions but, at DNA inputs <1 ng estimating proportions for SNP and STR markers by counting reads is less reliable.In this study, we present the results obtained from a single shotgun DNA library made from contrived mixtures of hair and blood target enriched using the STR, SNP and mtDNA probe capture panels, demonstrating proof of concept for application to forensically relevant degraded and mixed samples.

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The CAC Seminar Planning Committee is looking for agencies interested in hosting a future annual CAC Seminar! For more information, please contact

the Seminar Planning Committee at [email protected].

Exhibitor Acknowledgements

A HUGE THANK YOU to all our exhibitors for helping to make this seminar possible!

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Raymond Davis,

CourtSkills

Exhibitor Acknowledgements

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Special thanks to all those who provided donations!

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Gloria NusseChristopher Tanforan

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Extra special thanks to Vincent Tanforan for creating our Sherlock Slug artwork!

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