Complete the sequence of DNA of the human genome

There are 35,000 human genes in our genome

The method at use in this project is called a hierarchical shotgun

There are 7 main steps to the method

Break down into a code of 150,000 letters

Each fragment was placed into a bacterial artificial chromosome

The E. coli inside the chromosome would replicate each DNA sample

Special enzymes were used to cut each clone into ‘fingerprint’ of fragments

Combined the clones into a longer strand

Mapped them onto the human chromosome

Broke down the cloned fragments into 1000-2000 based pairs long

Generated a sequence of the individual bases

Shotgun method was used to sequence the fragments of human DNA

Overlaps of the letters of the genetic code were used to assemble the sequence of DNA

The first major experiment to map the human genome is performed on a sheep named Dolly

Deeper concepts were found in this experiment

Map out the single gene that causes genetic diseases or disorders

Discoveries in the medical fields

Cures of diseases

Costs 3 billion dollars

High risk in the project

People will not got a chance to live there lives fairly

DNA is going to determine the opportunities

Someday will be able to tell the life someone is going to live by there human genome