Biovac 13ROperating Instructions

2 Biovac 13R

Operating environment of instruments

In order to extend the machine’s service life, please pay attention to the following factors which may damage the machine:

• EnvironmentalConditions;• Effectsofenvironment,includingnaturalultravioletradiation(ie:DirectSunlight)• Erosionandabrasionoftheprotectivecoverandothersafetyparts.

• Indooruse;• Altitude:<2000m;• Ambienttemperature:+5°C~+35°C;• Relativehumidity:<80%;• Operatingvoltage:230V,50/60Hz;• Pleaseensureadequateventilationinareaofuseandappropriatesealingmeasurewhenperformingthe separationofpoisonoussubstancesandpathogenicmicro-organismcells;• Ensurethereisnovibrationand/orastrongdrafttoinfluencetheperformance.• Ensurethereisnodustorflammableandcorrosivegaspresentintheambientair.

Please read this manual carefully when using the instrument for the first time!The maintenance of the instrument should be carried out by the agents authorized by Biovac.

The following materials should not be used in conjunction with this instrument:

• Flammableandexplosivesubstances;• Strongchemicalmaterials;• Toxicorradioactivesubstances,• Pathogenicmicroorganisms

TheBiovac13RHighSpeedRefrigerationBench-topCentrifugecanbemaintainedonlybyqualifiedserviceengineersusingappropriatetools.

Shouldtherebeaproblemnotmentionedinthismanual,pleasecontactaBiovacauthorizedagentforthecorrectprocessingmethods.

OnlyaccessoriesprovidedbyVacutecshouldbeused.Ifotheraccessoriesareused,Vacutecwillnotbeheldresponsibleforanydamageorinjurytoinstrumentoroperatorasaresult.

TheBiovac13RHighSpeedRefrigerationBench-topCentrifugeshouldbeinspectedandmaintainedatstatedintervals.

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1. Safety clauses

1.1 Operating requirements Biovac13RHighSpeedRefrigeratedBench-topCentrifugeisdesignedaccordingtopresenttechnical

standards.Thereforeitmustbeusedaccordingtothedesignrequirements.WhentheBiovac13RHighSpeedRefrigeratedBench-topCentrifugeisusedaccordingtothefollowingimproperorinappropriatemethods,equipmentmybedamagedortheoperatorinjured. • Notusedinaccordancewiththedesignrequirements/intendeduse. • Theoperatorandmaintainerhavenotbeentrainedinitscorrectuseandoperation. • Impropermodificationstothedesignwithoutauthorisation. • Failuretoreadtheregulationsforsafeuse.

PersonswhouseormaintaintheBiovac13RHighSpeedRefrigeratedBench-topcentrifugeshouldreadandunderstandrelatedusageandsafetyregulationsinthisOperatingManual.

Thefollowingregulationsshouldbeobeyedtoavoidaccidents: OperatingManualshouldbekeptneartheBiovac13RHighSpeedRefrigeratedBench-topCentrifugeforreferencebyoperators.

Biovac13RHighSpeedRefrigeratedBench-topCentrifugeisdesignedtoseparatebiologiccells,organismsandbacteria.Thedensityofthesampleshouldnotexceed1.2g/cm3atthemaximumspeed.

Duringoperation(whenthesampleisbeingseparatedorrotorsarerunning),operatorsanddangeroussubstancesshouldmaintainadistanceofatleast30cmfromthecentrifuge.Theventilationholeofthecentrifugeshouldnotbecloggedbysubstances.

IfthefollowingsafetyprecautionsarenotobeyedwhenusingtheBiovac13RHighSpeedRefrigeratedBench-topCentrifuge,operatorsorotherpersonsmaybehurtorthecentrifugeandsamplesmaybedamaged:

• Thecentrifugeisnotcorrosionprooforimplosionproof,thereforeitshouldbeusedinacorrosion andimplosionfreeenvironment.

• Thefollowingmaterialsareprohibited: Flammableandexplosivesubstances: Strongchemicalmaterials; • Toxicorradioactivesubstances,orpathogenicmicroorganisms. • Corrosivesubstancesmaydamagematerialsinsidethecentrifugeandtherotors,thereforethey shouldbeputinsidesealedcontainerswhileseparating.

1.2 Precautions • Beforerunning(whenseparatingsamples),appropriaterotorsmustbefixedfirmly; • Duringoperation(whentherotorisrunning)orafterthecentrifugehasbeenstopped(therotoris

stillrunning),openingthelidbyhandisstrictlyforbidden. • AccessoriesmustbeprovidedandapprovedbyVacutec.Somecommonparts,suchasglassand plasticcontainersforseparating,shouldbeVacutecapprovedandbeinaccordancewiththe requirementsofthehighestspeedandmaximumcentrifugalforceofrelatedrotors; • Donotusethecentrifugeorseparatesampleswhilethelidisopen; • Changingmechanicalpartsandelectronicsshouldbecarriedoutbyqualified Vacutecpersonnelonly; • Whenusingthecentrifuge,usetheappropriaterotorsaccordingtoload.DoNotoverload; • Maintenanceshouldbecarriedoutperiodically.See“Cleanandpurify”chapterformore information.

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2. Introduction2.1 Layout of the Biovac 13R High Speed Refrigerated Bench-top Centrifuge

Front and Back view of Centrifuge

Descriptionofabovediagrams: 1. Frontcasingcover 2. ControlpanelanddigitalLCD 3. Lid

4. Observationhole 5. TopPanel 6. Frontventilation 7. Emergencyopen/Forceopen 8. Foot 9. Rearcasingcover 10. Rearventilation 11. PowerjackandOn/Offswitch

2.2 General Biovac13RHighSpeedRefrigeratedBench-topCentrifugewithmultifunctionprotectionintroduces

advancedtechnologysuchasspeedadjustmentviafrequencycontrol,microcomputercontrol,touchbutton,dialsetup,digitalLCD.Threerotorvariationsareavailable(fordetails,see“Table1:Typeofrotorandtechnicalparameters”).Thiscentrifugeisusedfortheseparationofbiologiccells,organismsandbacteria.

2.3 Device structure 2.3.1 The Device Lid

Onlywhenthelidlocksarelockedonboththeleftandrightofthelidmaythecentrifugerun; otherwisethewarningsystemwillsoundanalert(alightwillcomeonandabuzzerwillsound) andthedevicewon’trun.

Toopenthelidpress“”(openbutton)onthecontrolpanel.Oncethelidrisestoacertain height,thelidhingeandspringswillsupportthelid. Ifapowercutoccursortheopenbuttonisnotresponding,thesamplemustberemoved.Referto

Speed/F

S/F

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chapter7.1foremergencyopeninginstructions. Whiletherotorsarerunningand/orthepowersupplyison,DONOTopenthelidmanually.

2.3.2 The Chamber Thechambermaintainsastableworkingtemperature.

2.3.3 The Rotor Fordetailssee“Table1:Typeofrotorandtechnicalparameter”.Therotorrotatesata predeterminedspeedtocreatetherelativecentrifugalforcerequiredtoseparatevarioussamples. Thecentrifugalforceisseveralthousandtimesmorethang(accelerationofearthgravity),itis therefore important to use and maintain the rotor carefully and safely!

2.3.4 The Refrigeration System Therefrigerationsystemisusedtocreatealow-temperatureoperatingenvironmentforthe centrifugechamber.Thetemperatureisautomaticallycontrolled.Thecentrifugeshouldbe operatedinanenvironmentwithaconstantambienttemperaturebetween5ºCto35ºCandan ambienthumidity≤80%.Optimumoperatingconditionscanbeachievedwithadequate ventilationaroundthecentrifugeinaroomwithambienttemperature controlledbyair-conditioning. Aheatsinkisinstalledinthefrontofthemachinewhereairflowandcirculationisessential.Any dustshouldberemovedfromtheradiatorwithabrushorcompressedaironceeverythree monthstoensureeffectiveheatdissipation.Thedigitaltemperaturesensorinstalledatthebottom ofthechamberisusedtomonitorthetemperatureinthechambercontinuously.

Thetemperatureinthechambermayvaryfromthatofthesamplesatfirst,andtake aperiodtoequalise.

2.3.5 The Control System The control system allows the setting of the rotor speed, running time, temperature and control of displaysystemandwarningsystemoftheentiremachine.Toensureproperrunningofthe machineandthesafetyoftheoperator,DONOTopenthefrontcasingofthedevice,asthemain controlcircuitboardandotherelectronicsareinstalledontothefrontcoverofthedevice.

2.3.5 The Display System Thedisplaysystemcanshoweverysettingparametersimultaneously,andcantrackanychange inrealtime.Inaddition,itdisplaysrelativesystemerrorsandwarnings.

2.3.5 The Warning System Thewarningsystemwillalerttheuserof“overspeed”,deviceoverheating,lidwarning,etc. Analertisindicatedbyaflashing,red,warninglightonthecontrolpanelandabuzzerwillsound simultaneously.Ifrunning,thedevicewillbestoppedautomaticallyandtheuserwillbeunable tostartasequenceagainuntiltheerrorhasbeenresolved.NOTE:usermaypressthe“Esc”button onthecontrolpaneltosilencethewarningbuzzer.

2.4 Safety Protection Device

TheBiovac13RHighSpeedRefrigeratedBench-topCentrifugehasaseriesofsafetyprotectiondevices:

• Themountingrackismadeofsteelplatingandtheprotectionsteelringismadeofsteelcasting.Theinternalbladderoftheinnerchamberisstainlesssteel.ThefrontframeismadeofABS.Thelidisexplosionproof.Thereisaviewportinthecenterofthelid.

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Onlywhenthecentrifugeisonandtherotorhascompletelystopped,willthe“”buttonopenthelid.

• OverspeedStop Thewarningbuzzerissoundedwhentherotorspeedexceedsthesettingspeedby200r/min.Therotorstopsautomaticallyifthespeedexceedsthemaximumratedspeedby250r/min.Waituntiltherotorstopscompletely,thenopenthelid.Restartoncetheerrorhasbeenresolved.

• Openingisstrictlyforbiddenduringoperation. If“”isaccidentlypressedduringoperation,thedevicewon’trespond,however,thewarningalarmis

indicated.

• Openinginthecaseofanemergency Iftheelectricityisofforthereisanurgentproblemwiththerotor,youmayopenthelidmanually

whenitisnotpossibletodosobypressingthe“”button(ENSURETHEROTORHASSTOPPEDCOMPLETELYFIRST,TOAVOIDINJURY).

DONOTUSETHEEMERGENCYOPENINGFACILITYUNNECESSARILY!

2.5 Technical Specifications

2.3.1 Technical Data

2.6 Settling the Centrifuge

2.6.2 Thisdeviceshouldbeputonaswing-outtable-boardofappropriaterigidity,andbefreefrom vibrationandawayfromanydirectheatsourceorsunlight.

2.6.2 Thereshouldbeaspaceof10-15cmaroundthedevicetoallowforheatemissionfromthe condenser.

2.6.3 Aftermounting,adjustthe4feetlevelstoensuretherequiredsupportonthetable-board.

2.6.4 RequiredPoweroutputforthepowersupply:AC230V,50/60Hz.

THEDEVICESHOULDALWAYSBEEARTHED.

2.7 Configuration

Thefollowingaccessoriesaresuppliedwiththedevice.(Seepackinglistfordetails)

Youshouldinspectthecontentswhenreceivingproductsandunpacking.Ifthereissomethingwrong,pleasecontactVacutec.

Speed/F

S/F

Temp. Time.

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3 Available optional accessories

ThestandardconfigurationoftheBiovac13RHighSpeedRefrigeratedBench-topCentrifugeincludes: 1xCentrifugeandaNo.#2201rotor.

Accessoriesalsoavailableonenquiryare: RotorsNo.#2202and#2203(Fordetails,see“Table1:Typeofrotorandtechnicalparameters”)

Table1:Typeofrotorandtechnicalparameters

TypeofRotor

Rotor Number

Angleoftheangle rotor

(º)

Rated/Maxspeed (r/min)

Maximum capacity (ml)

Max centrifugal radius(cm)

Rated/Maximum centrifugal

acceleration (xg)

AnglerotorFS15.02 #2201 45º 13,800/

15000 24x2.0 8.2 17,459/20627

AnglerotorFS15.002 #2202 45º 13,800/

15000 64x0.2 7.2 15,330/18112

AnglerotorFS15.05 #2203 45º 13,800/

15000 16x5.0 8.9 18,949/22388

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4.Preparationsbeforeuse4.1Transportationandinstallation Biovac13RHighSpeedRefrigeratedBench-topCentrifugeistransportedinspecial-purposecartonwith

protectionmaterialsforbufferinginside.Afteropening,theprotectionmaterialsforbufferingshouldberemoved.

• Thenetweightofthedeviceisabout47kg,thereforeitisnecessarytoliftthedevicefrombothsides(theleftandright)tokeepitsbalance.Thefrontcovershouldnotbepressedonorusedforlifting.

• Keeplevelandavoidvibrationwhentransporting• Pleaseusespecialpackingcasesduringlong-distancetransportation.Keeplevelandhandlewith

care!

4.2ProperplacementoftheCentrifuge Biovac13RHighSpeedRefrigeratedBench-topCentrifugeisforindooruse,andtheworkareashould

meetthefollowingrequirements:

• Asafetyclearanceof30cmmustbemaintainedaroundthecentrifuge.Whilerunning,thereshouldbenoindividualorhazardoussubstanceincloseproximitytothecentrifuge.

• Theadapterortabletopshouldbesolidtoavoidvibration;ifmobileracksortrolliesareused,theyshouldbeequippedwithlockingdevicestoensurethesafeoperationofthecentrifuge.

• Ifthecentrifugeisplacedbesidethewallorinacorner,thereshouldbeaspaceof10cmbetweenthebackofthecentrifugeandthewalland15cmbetweeneachsideandthewalltoallowsufficientventilationandheatemission.

• Thecentrifugeshouldbeplacedawayfromwindowstoavoidtheheatfromdirectsunlight.• Thefoursupportinglegsofthecentrifugeshouldstandevenlyona“swing-out”table-board.• Thecentrifugeshouldbeputinacleanroomwithaconstantambienttemperaturebetween5ºCand

35ºCandanambienthumidityoflessthan80%

4.3Fixedplacementofequipment Afterthecentrifugehasbeenplaced,don’tmoveitfrequently.Alwaysensurethecentrifugestandslevel

onthetable-board.Theshouldbenovibration.

Toavoiddamagetotherotoraxis,pleaseremovetherotorwhenmovingthecentrifuge.

4.4Properconnectionofelectricsupply

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Theeachoftheelectricplugconnectedtothecentrifugemustbeingoodcondition.Thevoltageandfrequencyofthepowersupplyshouldbeinaccordancewiththerequirementsstatedinthetechnicaldescriptionorspecificationslabelledonthenameplateofthecentrifuge.Thepowerjack/switchisattherearofthemachine,“I”foronand“O”foroff.Onlyusethepowercableincludedwiththemachine.

Neverpress“▷/☐”or“QTemp”buttonimmediatelyafterstartup;thedoormustbeopenedfirsttoremovetherotorfixingexpandedpolyethylene(EPE)androtor,andthenthechamberandrotormustbeinspectedandcleanedbeforerunning.

5. Direction for use and operation5.1 Control panel and display interface

ShownasFig.6,controlpanelisdescribedinbriefasfollows:

1-Datadisplaywindow 2-Cancel/Exitbutton 3-Quickaccessbuttonofcommonparameters 4-Menubutton 5-Quicktemperaturebutton 6-Dooropeningbutton 7-Inchingbutton(quickstart;pressthebuttonandrotorspeedwillquicklyincreasetoitsmaximum allowablespeed;releasethebuttonandrotorwillquicklystop) 8-Start/Stopbutton 9-Dial(Selectandmodifysettingparameters;actsas“Enter”buttonwhenpressedin) 10-Speedandcentrifugalforcesettingbutton 11-Curvesettingbutton 12-Temperaturesettingbutton 13-Timesettingbutton

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ShownasFig.7,maininterfaceisdescribedinbriefasfollows:

1. Thetoprowisthestatepromptarea.“”indicatesdoorisinlockedstate;“”indicatesdoorisin unlockedstate;“”indicatesthebuzzerisinnormalworkingstate.

2. Thesecondrowisthespeed/centrifugalforcedisplayarea:theleftismaindatadisplayandthe rightiscorrespondingauxiliarydatadisplayafterconversion.Whentherotorspins,real-timespeed isdisplayed;whentherotorstops,“0”isdisplayed.Whencentrifugalforceisauxiliarydataandthe rotorisnotidentified,displayedcentrifugalforceis“-----”.

3. Thethirdrowisrunningtimeandchambertemperaturedisplayarea:whentherotorisnotrunning, displayedrunningtimeis“00:00”;whentherotorisrunningthedisplayedvalueistheremaining runtime.Displayedtemperatureisreal-timechambertemperaturevalue.

4. Thefourthrowissetparameter/alarmmessagedisplayarea:whenthesystemisinnormaloperation thedisplaysare:SetSpeed/Centrifugalforce;Setruntime;Settemperature;SetcurveandCurrent identifiedrotornumbersuccessivelyfromlefttoright.Whenasystemerroroccurs,therelevant errormessageisdisplayed.

5.2 Start Connectthepowercablesuppliedwiththemachineintothepowersocketontherearpanelofthe

machineandconnecttheotherendintomainssupply.ThepowersupplyusedforthemachineisAC230V,50/60Hz.Oncepluggedin,turnonthepowerswitchnexttothepowersocket.Thebuzzerwillsoundandthesoftwareversionwillbedisplayed.Oncetheself-checkiscompleted,itwillenterthemaininterface(asshowninFig.7),thenextoperationcannowbeconducted.

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5.3 Open Whilethepowerswitchisturnedonandtherotorhascompletelystopped,press“”button(door-

openbutton)onthecontrolpaneland“”willappearinthedisplaywindow;thelidwillautomaticallyopenviaspringaction,andthelidneedstoberaisedtherestofthewayandleanedbackwardagainsttheframe;theinnerchamberwillnowbevisibleandtheiconrepresentingdoorstatusonthemaininterfacewillbedisplayedasunlocked.

ATTENTION:whenthedoorisopened,thelidwillautomaticallybounceuptoacertainheight,keepclearofthelidduringthisoperationtoavoidinjury.Inthecaseofanerror,wherethedoorcannotbeopenedautomaticallyandthesamplesinthechamberneedtoberemoved,thedoorcanbeopenedmanuallybythemethodstatedinchapter7.1

5.4 Close Presstheliddownuntilthedoorhookontheliddrivesthelockingdeviceandyouhearthelockclick;

whentheicondisplaysas“”,itindicatesthatthedoorhasbeencorrectlyclosedandlocked. LidAdjustment:whenclosingthelid,ifyoufeelthelidistootightortooloose,unscrewthelocknutonthedoorhookwitha17mmsocketwrenchandthenadjustthedoorhookappropriately;turnclockwisetotightenandanti-clockwisetoloosen.Aftercompletingtheadjustment,tightenthelocknutagain.

Whenclosingthedoor,anappropriateforceshouldbeappliedtopresstheliddowntoavoiddamage to the door lock.

5.5 Installation/Dismounting of the rotor Ifinappropriaterotorandadapterareused,itwillcauseanunsatisfactoryseparationeffectand

could also damage the centrifuge.

TherotorusedmustbeaproductconfirmedandappointedbyVacutec,otherwiseitmaypotentiallycauseafault.ThespecificationandmodelofalltypesofrotorsconfirmedbyVacutecareprovidedinthismanual(fordetailsonspecificationandmodel,seeTable1“RotorTypeandTechnicalParameters”).

Wheninstallingrotors,usethe5mmhexagonspannersuppliedalongwiththemachine. Installation steps for rotors are as follows:

• Turnonpowerswitchandwaitforself-checkcompletion. • Press“”buttonandopenthelidofthecentrifuge;makesurethechamberisclean andimpurityfree;

• Cleanexternalsurfaceofmotorshaftandinspectthesealringonthemotorshaftensuring itisintact; • Preparetherotoryouwanttouse;holdtherotorwithbothhandsandalignthecenterholeofthe rotorwiththemotorshaft,andthenverticallyloweritontotheshaft; • Screwonthelocknutbyhandandthenusethe5mminnerhexagonspannertotighten thenutclockwise; • Rotorinstallationiscompleted.

Beforeuse,especiallybetweenprolongedperiodsoftime,theseatingoftherotorshouldbeinspectedforanychanges;whennecessary,thelocknutshouldbere-tightenedtoensuretherotorissecure.

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5.6Maintenanceofmotorsupportshaft Maintenancestepsofmotorsupportshaft: • Turnonpowerswitchandwaitforself-checktocomplete • Press“”buttonandopenthelidofthecentrifuge;thendismounttherotorusing5mminner

hexagonspanner.NOTE:tightenclockwiseandloosenanti-clockwise. • Removedust,impuritiesandseparatedsampleresidueinthecentrifugechamberensuring

itisclean. • Cleantheconicalsectionandthescrewthread(showninFig.8)ontherotatingshaft,ensuringno

dirtordustremains;thenevenlyapplythelubricatinggreasesuppliedalongwiththemachine.

5.7 Calculation of rotor load Calculationofmaximumload:

Biovac13Rdeskcentrifugewillgenerateahighcentrifugalforceduringhigh-speedrunning;eachrotorisdesignedtohaveenoughmechanicalstrengthtowithstandthisforcewhenworkingattheratedmaximumspeed,buttoavoidaccidentorinjury,therotorloadmustnotexceeditsmaximumratedload.

Whenseparatingsamples,thesamplesarefirstplacedintoacontainerandthenplacedtogetherintoa rotor, the sum total of the container and sample must not exceed maximum rated load of the rotor, otherwiseyoumustreducethesampleweightorcalculatepermissiblerunningspeedofrotor(NPERM)toensuretherotorloaddoesnotexceeditsmaximumratedload.

Calculationmethodofpermissiblerunningspeed(NPERM)ofrotorisasfollows: NPERM=NmaxX(maximumpermissibleload÷actualload)0.5

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Where: Nmax=maximumratedspeed; Maximumpermissibleload=maximumpermissibleload; Actualload=actualload

Don’tusetherotorunderoverloadedconditions,asthismayresultinseveredamageorinjurytotheequipment or operator.

5.8 Sample filing in centrifugal container Whenthecentrifugeruns,ifbalancedcorrectly,separationzoneofsampleswillnotinterferewitheach

otherduetotheabsenceofvibration,resultinginanimprovedcentrifugaleffect.Therefore,samplesshouldbeplacedintothecentrifugalcontainerassymmetricallyaspossibletoachievethebestbalanceduringthesequence.

Carefullyinspectthecentrifugalcontainer(centrifugetube,etc)beingused,ensuringitisinaccordancewiththeallowablemaximumratedacceleration(centrifugalforce);alwaysusethelowestrunning speed possible.

Pay strict adherence to the service life of the centrifugal container in use; especially when frequently runningundermaximumpermissibleload(speedandtemperature). Ifdamaged,itshouldbereplacedimmediately!

5.9 Safe use of rotor • Beforetherotorruns,sampleshouldbeloadedcorrectlyandsymmetrically.

• Whenexchangingarotor;usethe5mmspannertoloosenthelocknutanti-clockwiseand removetherotor. • Neverstartthemachinebeforethelocknuthasbeentightenedontotherotatingshaft. • Ifthecentrifugewillberunrepeatedly,therotorlocknutmustbeinspectedaftereachruntoensure itisstilltight.Ifthelocknutisloose,itmustbetightenedbeforeproceedingwiththenextrun. • Centrifugaltesttubesdonotneedtobeloadedsimultaneously,butdoneedto beloadedsymmetrically.

5.10 Parameter setting ATTENTION:

• Beforesettingparametersviathecontrolpanel,therotormustbecorrectlyinstalledon therotatingshaft. • Buttonsaretouchsensitive.Simplypresstherelevantbuttonfortherequiredoperation. • Iftheparametersareinsertedincorrectly,theycanbereset.

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Turnthedialwhileinthestandbyinterfacetoentertheparametersettinginterface;continuetoturnthedialtoswitchbetweentheparametersthatneedtobeset;whentherespectiveparameteritemisselected,agreenborderwillflickeraroundit.Afterselectingtherequiredparameter,pressthedialintoconfirmyourselectionandentertherequiredparameter.

5.10.1 Setting of Speed:

SettingrangeofspeedofBiovac13Rcentrifuge:minimumspeed:300rpm;maximumspeed:13800rpm.

Description: 1. Whileinthestandbyinterface,turntehdialuntilthespeedsettingzoneflickersasshowninFig.10.

Press[S/F]buttontoquicklyselecttheappropriateparametersetting. 2. Pressthedialtoconfirmandenterthespeedsettinginterface;onceintheinterface,press[S/F] buttontoswitchbetweenspeedselectionandcentrifugalforceselection.Theselectedparameter itemhasaflashinggreenborderindicatingthatthenumericalvaluecanbemodifiedandset;non- selecteditemsaregreyedout,shownasFig.10.Iftherotorisnotidentified,centrifugalforce displayis“-----”. 3. Turnthedialtochangethenumericalvalue.Speedissetbysteppedvariableadjustment:change invalueis100ateachturnofthedial.Iftherotorhasbeenidentified,thecorrespondingvaluewill beautomaticallyadjustedrelativetothevalueinsertedforthedesiredspeed. 4. Adjusttotherequiredspeedvalueandpressthedialtosavethesetting;youcanalsopressthe“Esc” buttontocancelthechangeandreturntothestandbyinterface.

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5.10.2 Setting of the centrifugal force:

SettingrangeofcentrifugalforceofBiovac13Rcentrifugerotor:minimumcentrifugalforce:100xg;maximumcentrifugalforce:22380g.

Settingrotorspeedcanbechangedbysettingthe“centrifugalforce”value;thespeedandcentrifugalforcewillnotcorrespondintheirrelationuntilaftertherotorhasbeenidentified(namelytherotornumber),centrifugalforceandrotorspeedwillthenconvertinrelationtoeachotherandthecurrentcentrifugalforcewillbedisplayed.

Settingmethod(asper5.10.1SettingofSpeed):

Calculation of centrifugal force Relative centrifugal force is usually thousands of times more than terrestrial gravity and it is the unit usedtomeasuretheefficiencyofdifferentkindsofinstrumentsseparatingorprecipitatingarticles.Calculationofcentrifugalforceisrelatedtocentrifugalspeedandcentrifugalradius;centrifugalforceiscalculatedbytheequationbelow: RCF=11.18X(n/1000)2Xr Where: r=centrifugalradius(cm) n=centrifugalspeed(rpm)

Attention: maximum centrifugal force value is only related to maximum speed and maximum centrifugal radius. The centrifugal force value “X g” displayed in the interface is calculated according to current rotor speed and maximum centrifugal radius.

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5.10.3 Setting of speed-up and speed-down curves:

Speed-upandSpeed-downtimecontrolofBiovac13Rcentrifuge:3groupsofspeed-upcurvesand3groupsofspeed-downcurvesareprovidedrespectivelyaccordingtodifferentrotorsandtheycanbefreelyarrangedingroups.3#curveismaximumspeedand1#curveisminimumspeed.

Settingmethod(asper5.10.1SettingofSpeed): 1. Turnthedialwhileinthestandbyinterfacetoentertheparametersettinginterface. 2. Inthecurvesettingmenuinterface,thecurvesettingboxisdisplayedbyagreenflashingborder. 3. Orsimplypress[Curve]buttontoquicklyselectthatparametersetting.

4. Turnthedialtochangethevalueofthecurvesetting 5. Thefirstrowofcurvesettingmenuisthespeed-upcurveandthesecondrowisthespeed-down curve.Pressthe[Curve]buttontoswitchbetweenthesetwooption.Adjusttothepreferedcurve andpressthedialtosavethesetting;orpressthe“Esc”buttontocancelthechangeandreturnto standbyinterface.

5.10.4 Setting of temperature:

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ChambertemperaturecontrolofBiovac13Rcentrifuge:minimumtemperatureaccessiblevarieswitheachtypeofrotoratdifferentspeeds.Settingrangeoftemperatureforthecentrifugeis-10~40ºC. Settingmethod(asper5.10.1SettingofSpeed) 1. Turnthedialwhileinthestandbyinterfacetoentertehparametersettinginterface. 2. Inthetemperaturesettingmenuinterface,whenthetemperaturesettingboxisselectedisdisplaysa flashinggreenborder. 3. Orsimplypress[Temp]buttontoquicklyselectthatparametersetting. 4. Turnthedialtochangethevalueofthetemperaturesetting. 5. Thesecondrowintemperaturesettingmenuisaswitchingdisplayindicatingchambertemperature andtubetemperature(displayedsampletemperatureisestimatedvalue,notactualvalue).“Chamber temp”ischambertemperatureand“tubetemp(simulation)”issampletemperature.Press[Temp] buttontoswitchbetweenthetwomodes.Adjusttotherequiredtemperatureanddisplaymode andpressthedialtosavethesetting;orpress“Esc”buttontocancelthechangeandreturnto standbyinterface.

5.10.5 Setting of running time:

SettingrangeofruntimeofBiovac13Rcentrifugerotor:1min~99h59min. Inthetimesetting,hour[H]andminute[M]canbesetrespectively. Settingmethod(asper5.10.1SettingofSpeed) 1. Turnthedialwhileinstandbyinterfacetoentertheparametersettinginterface.Onceinthetime settinginterface,tehtimesettingboxisdisplayedasselected,indicatedbyaflashinggreenborder. 2. Settingsequenceoftime:minute->hour->saveandexit.Thewhitenumericalvalueiscurrent adjustedparameter.Oncethevaluehasbeenadjusted,pressthedialtoselectthehoursetting.Press [Time]buttontoquicklyselectparametersetting. 3. Turnthedialtoswitchbetweenthevalues. 4. Thesecondrowoftimesettingmenuisruntimemodeoption;“countdown@start”iscountdown atstartuptime;“countdown@sets/f ”iscountdownaftercurrentspeedreachesthesetspeedor presetcentrifugalforce.Press[Time]buttontoswitchbetweenthetwomodes. 5. Adjusttherequiredtimeanddisplaymodeandpressthedialtosavethesetting;orpress“Esc” buttontocancelthechangeandreturntostandbyinterface.

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5.10.6 Reading and storage of user setting data

Biovac13Rcentrifugeprovides3groupsofsettingdatasavedforusers.Userscansaveandreadtheirmostcommonparametersettings. Settingmethod(asper5.10.1SettingofSpeed): 1. Press[P1]/[P2]/[P3]buttonwhileinthestandbyinterfacetoquicklyenterthe parametersettinginterface. 2. Turnthedialinthesettingmenuinterfacetoswitchthe“Read/Save”function;theselecteditemis indicatedbyaflashinggreenborder. 3. Whenswitching“Read/Save”,correspondingdataisdisplayedintheupperparameterdisplaybox. Forexample,whenselecting“loadfrompreset”,tehdatadisplayedintheupperdisplayboxis thedatathattheuserhaspreviouslysaved;pressthedialandthedataisreadouttocovercurrent setparametervalue;whenselecting“savetopreset”,thedatadisplayedintheupperdisplayboxis thecurrentdata;presstehdialtosavethedata.Press[P1]/[P2]/[P3]toswitchbetweenthe 3groupsofdata. 4. Adjusttherequiredtimeandpressthedialtosavethesetting;orpress“Esc”buttontocancelthe changeandreturntostandbyinterface.

5.10.7 Parameter change during the run process

Duringtherunofthecentrifuge,thespeed,temperature,time,centrifugalforceandotherparameterscanbechanged;oncenewparametersareentered,displayscreenreturnstomaininterfaceandthenewparameterscomeintoforce.

19Biovac 13R

5.11 Start centrifugal separation Oncethesettingofuserparametersiscomplete;rotorshavebeeninstalled;thesampleforseparation

hasbeencorrectlyinserted;thelidofthemachinehasbeencorrectlyclosed,thenthemachinecanbestartedtobegintheseparation.

Attention: Indication whether the lid of the machine is closed correctly is displayed on the main interface via the door lock status icon.

5.11.1 Press “ “ to start

1. Pressthestartbutton““onthecontrolpanelandtherotorwillfirstbeidentifiedata lowspeed;onceidentified,thecorrectsetspeedorcentrifugalforcewillbeenforced. 2. Therotorwillbeacceleratedtothesetspeedaccordingtothesetspeed-upcurve. 3. Runtimeisdisplayedby“countdown”methodduringtherunprocess;whenruntimedisplay reacheszero,namely“00:00”,thecentrifugestopsandtheoperationiscomplete 4. Chambertemperaturequicklydropsatthestartofthecompressor;oncethechamberreachestheset temperature,thecompressorswitchesoff. 5. Real-timespeedandcentrifugalforcearedisplayedduringtherun;displayedcentrifugalforceis acquiredviaautomaticconversioninrelationtotherotordetectedbythecentrifuge. 6. Ifrotorspeedorcentrifugalforceexceedsitsmaximumpermissiblevalueorsetvalueduring therun,analarmwillsound;whenmotortemperatureprotectionistriggeredorcompressorover voltageprotectiontriggers,analarmwillsoundandprompttheusertomakethe relevantprocessingadjustment.

Attention:fordetailsonalarmpromptmessagecontent,see“Troubleshooting”. 7. Thelidofthemachinemustnotbeopenedwhiletherotorisstillturning.

5.11.2 Press “ “ button to start

Pressandhold““buttonandthesystemwillidentifytherotor;onceidentified,therotorspeedwillquicklyincreasetopermissiblemaximumspeedonthemachine;release““buttonandtherotorwillquicklyslowuntilitstopscompletely.

5.12 Stop Separation

Oncethecentrifugebeginsitsrun,itcanbestoppedbythetwomethodsbelow: 1. Undernormalconditions,theruntimeofthecentrifugehasbeenpreset,andwillautomaticallystop

aftercountingdowntozero.

2. Ifyouwanttostopthecentrifugeduringtheprocessofanormalrun,pressthestopbutton““onthecontrolpanelatanytime,andthecentrifugewillstoprunningimmediately.TheCountdowninthedisplaywindowindicatingtheruntimewillstopsimultaneously.

Onlyoncetherotorspeeddropstozero,i.e.speeddisplaywindowdisplays“0rpm”andcentrifugalforcedisplaywindowdisplays“0xg”,youcanpresstheopenbutton““onthecontrolpanelandthelidwillpopopenviaaspringaction,thelidcanthenbeopenedbyraisingitbyhand;thedoorlockstatusicononthemaininterfacedisplaysthelockasopen,theseparatedsampleinthecentrifugecannowberemoved.

20 Biovac 13R

5.13 Temperature Control

5.13.1 Pre-Refrigeration Function

Whenthelidofthecentrifugeisclosedandpriortherunningofthepresetprogramme,oraftercentrifugalseparationiscomplete,thecentrifugewillenter“pre-refrigerationmode”accordingtothesettemperature.Inthisway,itcanreachtherequiredtemperaturepriorcentrifugalseparation;andmaintaintheappropriatetemperatureafterseparationtoprotectsamples.Ifambienttemperatureorchambertemperatureishigherthanthatofthesample,usersareadvisedtopre-refrigerateandtheninsertsamplesforcentrifugalseparation,otherwisethe“TEMPOVER40ºC”warningmayappear.

Attention:Pre-Refrigerationfunctionwillstopafter1hour;Itisresetbyopeningandclosingthedooragainorduetopowerfailure.

5.13.2 Quick temperature function

Thecentrifugehasaquicktemperaturefunction.Firstsetthetargettemperatureparameterandexpectedtimedurationandmakesuretherotoriscorrectlyinstalledandlocked;closethelidandthenpressthe<Qtemp>buttontostartthequicktemperaturefunction,andtherotorwillspin.

Ifsettemperatureishigherthanchambertemperature,thecompressordoesnotstartandheatisgeneratedbyfrictionbetweenthehigh-speedrunningrotorandair,toquicklyreachthetargettemperature.

Ifsettemperatureislowerthanthechambertemperature,therotorrunsatthespecifiedspeed;thecompressorwillstartandtherefrigerationsystemstartstoquicklyreducethechambertemperaturetoreachthetargettemperature.

Afterthetemperaturereachesthesetvalue,therotorcontinuesrunning,butmainparameter<Qtemp>ondisplayscreenwillflickerandsimultaneouslybuzzersoundsintermittentlytoremindtheuser

21Biovac 13R

thatquicktemperatureiscomplete.Pressthestopbuttonandthequicktemperaturefunctionwillbeterminatedandtherefrigerationandrotorwillstop.

5.13.3 Temperature control in the separation process

Whenthecentrifugeisstarted,thetemperaturecontrolsystemwillrunforaperiodoftimeuntiltherequiredtemperatureisreached.Thetemperatureinthecentrifugalchamberwillthenbestablewithin±2ºCrangeofsettemperature.

5.14 Running record

In order to ensure normal operation of the machine and extend the service life of the machine, and accumulateinformationaboutthemachineoperationtoassistinthediagnosisoffaultsoraccidents,pleasecarefullykeeparecordofeachrun(onebookforeachmachine).

Belowistherecommendedoperationrecordtable:

RECORD TABLE

Date of test

Name of sample

Weight of monotube (including sample)

Rotor No.

Rotory

speedTime Temperature Working

condition Operator

22 Biovac 13R

6. Maintenance

6.1 Cleaning and sterilization

Usershavetheresponsibilitytocleanandpurifythecentrifugewhendangeroussubstancesspilloverintothemachine.Usersshouldcleanandpurifyitaccordingtothemethodrecommededbythemanufacturerstoensurethatthemachineisnotdamaged.

Inappropriate detergents and incorrects methods of cleaning may do harm to the centrifuge or internal parts. Cleaning and sterilization should be carried out according to operating procedures described in the operating and technical instructions.

6.1.1 Implementation of cleaning and sterilization

Turnoffthepowerandremovethepowercablebeforecleaning!

Thecentrifugeshell,rotorchamber,rotorandcentrifugalcontainersusedandsoonshouldbecleanedandmaintainedregularly;thuscontaminationcanbeclearedavoidingdamagetothepartsusedandapollutedenvironment.Ifyouarenotclearonwhichcleaningagentsshouldbeusedforcleaningthecentrifuge,pleasecontactVacutec.

• Organicsolventsareforbidden,astheycandegreasethebearingsofthemotor; • Liquid,especiallyorganicsolventsshouldnotcomeincontactwiththesupportingshaftandball

bearingswhenbeingcleaned; • Ifthereisfrostorathinicelayer,theyshouldbewipedawayoncetheyreverttowater.

6.1.2 Implementation of high-pressure sterilization

• Rotorsusedbythiscentrifugecanendurehigh-pressuresterilization.Temperature:121ºCwith apressureof250kPa,maintainedforatleast10minutes.Pleaserefertotheinstructionsofferedby manufacturersvariousseparatingcontainers.

• Alwaysconfirmwhetheritispermissibletosterilizerotorsandcontainerswithhigh-pressure sterilizationmethod.Thetemperatureanddurationofhigh-pressuresterilizationshouldbestrictly controlledaccordingtothedifferentrotorsandcontainersusedforseparating;

• Dontusearotororcontainerforseparatingiftheyarecorrodedordamaged.

6.1.3 Maintenance

6.1.3.1 Donotbringtherotorintocontactwithsharpobjects.Whenmovingordisassemblingthe centrifuge,youshouldtakecarenottodamagetherotor,asscratchesandindentationsmaycause cracksduringoperation.

6.1.3.2 Regularlycheckforspots,groovesorminutecracksintherotor(especiallyatthebottomofthetest tube).Ifanyarefound,stopusingthemimmediatelyandcontactVacutec.

6.1.3.3 Therotorshouldbecleanedonceeveryweekundernormaloperation.Rinsetherotorimmediately ifitsisusedtoseparatesalinesolutionorothercorrosivesamples.Ifliquidissprinkledontothe rotor;blotitupandrinsethepartimmediately.

23Biovac 13R

6.1.3.4 Pleasecleanwithspongesorcottonclothdippedinneutralcleaningsolution,thenrinsethe cleaningsolutionoffwithdistilledwater.Don’tsprinkleorspraywaterontotherotor;becauseit maycausetherotortocorrodeduetoresidualwater.Inverttherotorandallowittodry.

6.1.3.5 Dustthecoolingplatesofthecondensatoronceeverythreemonths.

6.1.3.6 Checktheprotectivecover

6.1.3.7 Inspectionofrotorpartsandsafetyconditions.

6.1.3.8 Confirmthedeviceisadequatelyearthed.

6.1.3.9 Removeanywaterorcondensationfoundinthechamberimmediately.

6.1.3.10 Turnofftheelectricityanddisconnectthepowercablebeforedisassembingthefrontcasingofthe device.Attention: This should be carried out by a trained Vacutec Technician only.

6.1.3.11 OnlysparesprovidedbyVacuteccanbeusedinthisdevice.

6.1.3.12 Turnofftheelectricitywheneverthecentrifugeisn’tinuse.

6.1.3.13 TransportationandStorage Thismachineisaprecisioninstrument.Water,vibration,tiltingandinvertedplacementarestrictly

forbiddenduringtransportationandstorage.

Please note the protection indicators on the packaging!

Fragile This Side UP Keep Dry Do Not Tilt

7. Trouble shooting

7.1 To open lid in case of emergency

Undernormalconditions,thefunctiontoautoopenthelidwillbedeactivatedwhentheelectricityisoffortheoperationtoopenthelidisdisabledbyaccident.However,samplesinthechambermayneedtoberemoved.Inthiscase,youmayopenthelidofthecentrifugemanuallytotakesamplesout.

Attention:Thisisforemergencyonly!

Intheeventofapowerfailure,thebrakewillnotengage,thereforetherotorwillcontinuetospinforaprolongedperiod.Pleasewaitforittostopcompletely(observethroughtheobservationholeattehtopofthelid)beforeattemptingtoopenthelidmanually.

Firstturnoffthepowerswitch;Anemergencydooropeningholeisfoundontheleftpanelofthemachineatthetopfrontcorner(shownas2inthebelowfigure)

24 Biovac 13R

Ascrewdriverwithadiameteroflessthan4mmoranyotherhardtool(shownas1inthebelow figure)canbeinsertedintotheholeandpushedfirmlytoreleasethedoorlock.Oncethelidsprings up,thelidcanthenbeopenedbyhand. Afterthelidhasbeenopened,themanualopenerwillautomaticallybereset.Thedoorcanonce againbeusedundernormaloperationonceelectricalpowerisrestored. Attention:thismethodistobeusedonlyinanemergencyandnotasaregularoccurance.

7.2 Fault alarm Message

Thefollowingtableslisttehalarmmessagesthatthemachinedisplaysandtherelevanterror explanationsandtroubleshootingsolutionstoassisttheuserinclearinganerror.

Ifusersareunabletocleartheerror,orthealarmmessagethatthemachinedisplaysisnotlistedin thefollowingtables,usersshouldimmediatelycontactsupportpersonnelatVacutec.

Fault Alarm Message Table

Fault ErrorexplanationandtroubleshootingsolutionsStartandnodisplayor sudden no display

1.Thereisnoelectricityorthecableisnotconnectedcorrectly.2.Inspectpowersocketandconnectionwhethertheyareingoodcondition;inspectpowersocketwhetherithaspower.3.Inspectpowerswitchandensurethereisgoodcontact4.Iftheerrorcannotbecleared,pleasecontactVacutec’ssupportpersonnel.

The Machine suddenly stops during a run

1.Exceededmaximumratedspeedofrotor:Oncerotorspeedexceedsitsratedspeed250r/min,overspeedalarmwillbesounded.Themachinemustbestoppedtoresetthespeed;2.Exceededthesetspeedofrotor:3.InvertermotoroverheatedorCompressoroverheated,thepowersupplyoftheinverterandcompressorinthemachinewillshutoff,sothemachinestopsrunning.4.Chamberovertemperature:Checksettemperaturewhetheritiswithin40ºC;checkpaneldisplaytemperaturewhetheritexceeds40ºC.Ifitistheformer,settemperatureshouldbelowerthan40ºC.Ifitisthelatter,chambershallbecooledtoroomtemperature.Butinanyevent,themachineshallstopcompletelyfirstandthenberestartedaccordingtotherequirement;5.Ifkeyboardpanelhasnodisplay,pleaseinspectthepowersupplysystemofthemachine;6.Voltagemaypossiblybetoolow,inspectpowersupplyvoltagewhetheritmeetstherequirement.

Thelidcannotbeopened

1.Therotorhasnotstoppedrunningcompeletlythereforethedoorwillnoopen.2.Openthedoormanuallyoncetherotorhasstoppedspinning);3.Iftheerrorcannotberesolved,pleasecontactVacutec’ssupportpersonnel.

25Biovac 13R

The machine vibrationisexcessive

1.Therotorisexceedingcriticalspeedandsomevibrationofthemachineisnormal;2.Inspecttherotorwhetheritislockedinplace.3.Inspectthesymmetryofrotorloadandensurethemachineislevel.4.Ensuretherotorisinstalledproperly;5.Inspectdriveshaftandrotateitbyhand;ifdriveshaftdoesnotrotatesmoothly,driveshaftormotormaypossiblyhaveafault.Iftheerrorcannotbecleared,pleasecontactVacutec’ssupportpersonnel.

Turnonpowerswitch,buttheindicatorlightondisplaypanelisoff.

1.Powersupplyisnotconnected;inspectthedistributionpowersupply.2.Iftheproblemcannotberesolved,pleasecontactVacutec’ssupportpersonnel.

Operating panel displaysabnormally

Itmaypossiblybecausedbygridinterference.Pleaseshutdownandwaitaminute,thenrestartit,anddisplayshouldbenormal.

Pressstartbutton,butrotor does not run

1.Shutdownandthenrestart2.Iftheerrorstillcannotbecleared,pleasecontactVacutec’ssupportpersonnel.

Refrigeration fails or the machine does not reach the preset temperature

1.Ambienttemperatureistoohigh;reduceambienttemperaturetobelow35ºC;2.Temperaturesettingisincorrect;resettheparameter.3.Iftheerrorcannotberesolved,contactVacutec’ssupportpersonnel.

Themachinegivesoffsmoke&oraburntodour

1.Shutoffpowersupply;2.Rotatedriveshaftbyhand;ifdriveshaftcannotberotatedsmoothly,itmayhaveafault.3.Iftheerrorcannotberesolved,contactVacutec’ssupportpersonnel.

Forfaultsnotmentionedabove

PleasecontactVacutec’ssupportpersonnelforassistance.

7.3 Safety protection

7.3.1 Automatic Stop protection function

• Safetyprotectionfor“Overspeed”:Iftherotationspeedoftherotoris250rpmabovetehratedspeed, theautomaticstopprotectionfunctionfor“Overmaxspeed”willcomeintoeffect.Theprompt message“OVERMAXSPEED”appearsandthebuzzersoundssimultaneously.

• Safetyprotectionfor“Overheat”:Ifthetemperatureinthechamberexceeds40ºC,theautomatic

stopfunctionfor“Overheat”willcomeintoeffect.Thepromptmessage“tempover40ºC”appears andthebuzzersoundssimultaneously.

• Whenruntimecompletes,thedevicestopsautomatically,andtheruntimeisdisplayedas“00:00”

26 Biovac 13R

• Procedureinthecaseofabnormaloperation:Ifthereisabnormalvibration,noiseorapeculiarsmell,shutdownthethedevice,switchofftheelectricity,removeanysamplesanddoaninspection.PleasecontactVacutecifthecausecannotbefound.

8. Technical Data

Function/Parameter Technicalindicators

Operating environment

•Environmentfreeofdustandvolatile orcorrosivegases;novibrationorairflowrestrictions to hinder the performance

•Altitude:≤2000m•Relativehumidity:≤80%

Ambienttemperature •+5ºC–+35ºCAppliedvoltage •AC230V,50/60HzRated power •480VA

Typesoffusesandparameter•F1,F2:250VAC,10A;•F3,F5:250VAC,0.5A;•F4,F6:250VAC,2A

Settimerange •1min-99h59minSettemprange •-10-40ºCSetspeedrange •300-13800rpm(canextendto15000rpm)Rated/Maximumcentrifugalforce(RCFmax) •17,459xg/22,388xgAscendingvelocity •Max(highest)ascendingvelocity,<5minDescendingvelocity •Minimum(lowest)descendingvelocity,<5min

Refrigerationeffect

•Whentemperatureofthecentrifugalchamberisequaltothatoftheambienttemperature,±15ºC-20ºC,atemperatureof-5ºCinsidethechambershouldnotbemaintainedforlongerthan30minat the rated speed

Completemachinevibration •vibrationofthecentrifugeshouldn’texceed0.1mm

Precisionofsustainedrotaryspeed •±1%

Timeaccuracycentrifuge •Timeaccuracyofcentrifugedoesnotexceed±1%

Noise(atthemaximumspeed) •Lessthan58dBatthemaximumspeedExternaldimensions(device) •525mm(L)*300mm(W)*350mm(H)Netweight •47kgExternaldimensions(Packaging) •660mm(L)*420mm(W)*630mm(H)

27Biovac 13R

Roto

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Roto

r typ

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17459/20627

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303-001

247.2

15330/18112

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246.4

13626/16099

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#2203

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16x5

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5216

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8.9

18949/22388

28 Biovac 13R

P.O. Box 48488, Roosevelt Park, 2129,South Africa.

Tel: Jhb 011 475 1823Dbn 031 301 7617CT 021 461 9607Fax. 011 475 1819

email: support@vacutec.co.za