biotech product guide

8/2/2019 Biotech Product Guide

1/34


2/34

Quality atmdi 02

Innovative Technologies for Nucleic Acid Purification 03

Plasmid DNA Isolation Kits 04

Selection Chart 04

Process Flow 05 - 07

FastLyse pDNA Miniprep Kit 08

pDNA Miniprep Kit 09

Express 96 Miniprep pDNA Kit 10

Quanta Midi Kit 11

Quanta Maxi Kit 12

Express Midi Kit 13

Express Maxi Kit 14

Genomic DNA Isolation Kit 15

Selection Chart 15

Process Flow 16

gDNA Miniprep Kit 17

Plant gDNA Miniprep Kit 18

PCR Purification & Gel Extraction Kits 19

Selection Chart 19

Process Flow 20-21

PCR Purification Kit 22

Micro PCR Purification Kit 23

Nano PCR Purification Kit 24

Gel Extraction Kits 25

Micro Gel Extraction Kit 26

Nano Gel Extraction Kit 27

Binding membranes for Molecular Biology 28

Membrane Syringe Filters 31

Bottle Top Filters 32

Contents Page


3/34

mdi

Certificate of Quality

Membrane Technologies

Head of Quality Control

Issue Date: 03-May-2010

Advanced Microdevices Pvt. Ltd.21, Industrial Area, Ambala Cantt, INDIA,

Tel: +91-171-2699290/ 2699274

Fax: +91-171-2699221/ 2699008Website: www.mdimembrane.com

Email: mdi @vsnl.com

An ISO 9001:2000 Company

The Fastlyse pDNA Miniprep Kit has beenmanufactured in mdi facility in compliance with ISO9001

regulations using validated productionprocesses.

LOT RELEASE CRITERIA

Binding Capacity of the column

: 50g

pDNAYields

DNAPurity(A260 /A280) : 1.8-2.0

FASTLYSE pDNA MINIPREP KIT

Catalog No. : FLPKXXXXXXX0250Type : FLPKLot No. :

DNase Free : Yes

Plasmid type pUC vectorsCopy number-High (500-700)Fragment size -9 kbElution volume 50l

VALIDATED FOR

Downstream Processing

Restriction Digestion Ligat ion PCR Sequencing Bacterial Transformation Cloning

Shelf Life

Minimum one year at Room Temperature More than two years at 2-8 C

Purification of low copy number plasmids (


4/34

Innovative Technologies for Nucleic Acid Purification

Page 03mdi PRODUCT GUIDEBIOTECH

mdi introduces a major breakthrough in the field of nucleic acid purification through

its latest research. These special kits offer extremely high yields in much reduced

process time while effectively addressing other user concerns such as purity, yields,

consistency and shelf life.

Unique Performance Advantages

uFastest pDNA isolation in 10 minutes.

uNo pellet formation and resuspension steps.

uWorks with very low culture volumes upto 600l.

uVery high binding capacity of upto 50g.

uVery high yields with low elution volumes.

uVery high within lot and inter lot consistency.


uFastest large scale pDNA Isolation in just 30 minutes

uLesser number of operating steps

uVery high binding capacity

uNo precipitation required for desalting

uHigher yields with less culture volumeuHighly concentrated, ultrapure pDNA isolation in very low elution volumes

uHigh within lot as well as lot to lot consistency


uFastest DNA Purification in just 5 minutes

uHigh DNA Recovery from even low feed quantities

uHighly concentrated DNA in very low elution volumes

uEasy purification of large sized fragments without shearing

uSuitable for all type of downstream applications

mdiFastlyse pDNA Miniprep Kits

mdiQuanta Kits

mdiNano PCR & Gel Extraction Kits

0

50

100

150

200

250

Culture Volume

35ml 100 ml

Yield(g)

Higher Yields with Less Culture Volume

Higher Yields with Less Culture Volume

0

2

4

6

8

10

12

14

16

0.61.5

Yield(g)

Culture Volume (ml)

mdi

Competitor Q

High Concentration Recovery

0

20

40

60

80

100

120

61.6 ng 107.6 ng 422.7 ng

Different Feed

Recovery%

Elution Volume : 5l

mdi

Competitor Q

New


5/34

Selection Chart

Plasmid DNA Isolation Kits

Plasmids are extra chromosomal DNA molecules capable of replicating

independently of the chromosomal DNA. This genetic material is ubiquitous in

bacteria and because of its special ability to move genes from cell to cell, has become a

versatile tool for both researchers and scientists involved in life sciences research.

There are several concerns related to researchers such as yields, purity, processingtime, binding capacity, consistency and shelf life.

mdipDNA isolation kits offer many unique advantages with respect to all these issues.

The following selection chart will help you choose the most suitable kit for your

application.

Page 04 mdi PRODUCT GUIDEBIOTECH

FastLyse pDNA MiniprepKit

1.8-2.0 600l 5 ml Upto 25g 10 minutes?In-Vitro Transcription

?In-Vitro Translation

?High quality sequencing

?Cloning

?Probe Generation

?PCR

?Restriction Digestion

?Bacterial Transformation

?Ligation

?Transfection

?Gene Silencing

?Microinjection

?Library construction

pDNA Miniprep Kit 1.8-2.0 1 ml -5ml Upto 25g 30 minutes

Express 96 pDNAMiniprep Kit 1.8-2.0 1 ml -5ml

Upto25g/well 45 minutes

Quanta Midi Kit 1.8-2.0 25 35 ml Upto 250g 20 minutes

Quanta Maxi Kit 1.8-2.0 100-130ml Upto 1000g 25 minutes

Express Midi Kit 1.8-2.0 50-75 ml Upto 200g


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Small Scale Plasmid DNA Kits

mdi Fastlyse pDNA Miniprep Kit

( upto 25g)

mdi pDNA Miniprep Kit

( upto 25g)

mdi Express 96 pDNA Miniprep Kit

( upto 25 g/well)

Plasmid DNA Purification Kits: Process Flow


Elute

Overnight BacterialCulture

Bind & Wash

Lyse & Neutralize

Ultra Pure pDNA

Elute

Dry

Overnight BacterialCulture

Bind

Wash

ResuspendLyseNeutralize

Ultra Pure pDNA

ResuspendLyseNeutralize

Pelleted Bacteria

Filter

ExpressFilter Plate

Bind

Vacuum

ExpressPrep Plate

Wash

Vacuum

Elute

Vacuum

Vacuum

Microtubesfor Ultra

Pure pDNA

Tube Rack


7/34


mdi Quanta Midi Kit

( upto 250g)

mdi Quanta Maxi Kit

( upto 1mg)

Pelleted Bacteria

Alkaline Lysis

Clear Lysate

By Filtration

Add Binding Buffer

Bind/Wash

VacuumPump

Dry

Elute

Ultra PurePlasmid DNA

Pelleted Bacteria

Alkaline Lysis

Clear Lysate

By Filtration

Add Binding Buffer

Bind/Wash

VacuumPump

Dry

Elute

Ultra PurePlasmid DNA

Large Scale Plasmid DNA Kits


8/34

mdi PRODUCT GUIDEBIOTECH Page 07

Resuspend Pelletin Solution AL-1

Add buffer AL-3 and wait

Filter intoMidi column

VacuumPump

Bacterial Pelletby centrifugation

Cell lysis inSolution AL-2

Pass clear lysateby applying

vacuum

Wash and dryagain by vacuum

VacuumPump

Eluteby vacuum

Add Buffer P

Pass throughprecipitator

VacuumPump

Dry and eluteby centrifugation

Ultra pure

concentratedPlasmid DNA

Resuspend Pelletin Solution AL-1

Add buffer AL-3 and wait

Filter intoMaxi column

VacuumPump

Bacterial Pelletby centrifugation

Cell lysis inSolution AL-2

Pass clear lysateby applying

vacuumWash and dry

again by vacuum

VacuumPump

Eluteby vacuum

Add Buffer P

Pass throughprecipitator

VacuumPump Dry and elute

by centrifugation

Ultra pureconcentrated

Plasmid DNA

mdi Express Maxi Kit

(upto 750g)

mdi Express Midi Kit

(upto 200g)

Large Scale Plasmid DNA Kits


9/34

ORDERING

INFORMATION

FastLyse pDNA Miniprep KitThe Next Level in pDNA Isolation ( In just 10 minutes)

High Yields

0

510

15

20

25

30

35

0.6 1.5 3 4.5

Culture Volume (ml)

Elution Volume: 50l

Yield(g)

Very High Binding Capacity

0

10

20

30

40

50

60

mdi Competitor Q

BindingCap

acity(g)

Type XX XX XX X Pack Size

Type Code Pack Size Code

FLPK FLPK 50 0050

250 0250

1000 1000

Example: FLPK XX XX XX X 0250


Unique Performance AdvantagesuFastest pDNA isolation in 10 minutes

uWorks with very low culture volumes (600l)

uNo pellet formation and resuspension steps

uVery high binding capacity of upto 50guVery high yields with low elution volumes

uVery high within lot and inter lot consistency

Unique TechnologyDirect pDNA isolation from 600l culture without any pelleting and resuspension.

A unique soft pellet formation step allows ideal lysis conditions resulting in very

high yield even with larger culture volumes upto 5ml, helps reduce process time

and makes it ideal for plasmid screening.

ApplicationsuLibrary construction

uRestriction Digestion

uCloning

uLigation

uBacterial Transformation

uPCR

uAutomated Sequencing

uProbe Generation

uMicroinjection

u

u

u

u

u

u

Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280

Specifications

Purity

pDNA Binding Capacity : 50 g

pDNA Yield : Upto 25 g

pDNA Size : Upto 25 kb

Minimum Culture Volume : 600 l

Elution Volume : > 30 l

Total Time Taken : 10 minutes


10/34

ORDERING

INFORMATION


pDNA Miniprep Kit(In Less Than 30 Minutes)

Unique Performance AdvantagesuHigh Binding Capacity > 25g

uHigh pDNA yields and Purity

uHighly Concentrated Yields in low elution volumes

uNo Precipitation required for desaltinguHigh within lot and lot to lot consistency

ApplicationsuAutomated Fluorescent Sequencing

uRadioactive Sequencing


uTransformation / Transfection

uCloning

uPCR

uLigation

uProbe Generation

uMicroinjection


Specifications

Purity

pDNA Binding Capacity : > 25 g

pDNA Yield : Upto 25 g

Culture Volume : 1-5 ml

Minimum Elution Volume : > 30 l




MIPK MIPK 50 0050

250 0250

1000 1000

Elution volume vs pDNA Recovery and Concentration

0

200

400

600

800

1000

0

10

2030

40

pDNAConc.(

ng/l)

pDNARecove

ry(g)

30 50 100 150

Elution volume(l)

pDNA Recovery

pDNA Conc.

Culture Volume vs. pDNA Recovery and Concentration

0

200

400

600

800

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7

Culture Volume(ml)

Elution Volume: 50l

pDNA Recovery

pDNA Conc.pDN

AConc.

(ng/l)

pDNARecovery(g)

Incubation time vs. Recovery

10

30

5070

90

110

1 2 3 4 5 10 30

Incubation time (min)

50l

100l%R

ecov

ery

Example: MIPK XX XX XX X 0250


11/34

ORDERING

INFORMATION


Express 96 Miniprep pDNA Kit(In Less Than 45 Minutes)



EMPK EMPK 4 0004

24 0024

High Consistency: Well to Well

0

4

8

12

16

20

12 24 36 48 60 72 84 96

Culture Volume: 1.5ml

Yield(g/well)

PurityUltrapure Plasmid DNA: A / A = 1.8-2.0260 280

Example: EMPK XX XX XX X 0024

Unique Performance AdvantagesuMultiple (96) samples processing

uVery high binding capacity

uNo precipitation required for desalting

uHighly concentrated, ultrapure pDNA isolation in very low elution volumesHigh within lot as well as lot to lot consistency

ApplicationsuRestriction Enzyme Digestion

uLibrary Screeining

uIn vitro Translation

uSequencing

uLigation

uTransformation / Transfection

uPCR

SpecificationspDNA Binding Capacity/Well : > 25 g

pDNA Yield/Well : upto 25 g

Culture Volume/Well : 1-5 ml

Minimum Elution Volume/Well : 75 l



12/34


Quanta Midi Kit(In Just 20 Minutes)

Type

Type Code

QDPK QDPK

XX XX XX X Pack Size

Pack Size Code

25 0025

100 0100

Example: QDPK XX XX XX X 0100

Unique high binding

capacity midi spin column

Vacuum based,

no gravitational wetting

Concentrated pDNA,

no Isopropanol precipitation

Unique Performance AdvantagesuFastest Midiprep for pDNA Isolation

uVery high binding capacity - Upto 400g

uNo gravitational waiting

uSimple filtration to remove cell debris; No centrifugationuNo precipitation required for concentration

uHigher yields with low culture volume

uHighly concentrated, ultrapure pDNA isolation in very low elution volumes


Unique TechnologyCombines the use of a specially designed filtration devices and tube extender to

obtain high purity pDNA in just 30 minutes. No desalting is required to obtain

ultrapure pDNA yields.

ApplicationsuAutomated Sequencing


uCloning

uTransfection / Transformation

uPCR

uLigation

uMicroinjection

uProbe Generation

Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280Purity

Yield & Binding Capacity

0

50

100

150

200

250

300

350

400

450

Yield

Binding Capacity

mdi Competitor Q

(g)

Specifications

Low Copy

Plasmid

High Copy

Plasmid

Binding capacity of membrane ( ds DNA)

Recovery

Maximum culture volumes

400 g 400 g

80% 80%

25-35 ml 50 ml

Expected yield of plasmid 150-250 g 100-200 g


13/34

ORDERING

INFORMATION

Quanta Maxi Kit(In Just 25 Minutes)


Unique Performance AdvantagesuFastest Maxiprep for pDNA Isolation

uVery high binding capacity - Upto 1200 g

uNo gravitational waiting

uSimple filtration to remove cell debris; No centrifugationuNo precipitation required for concentration

uHigher yields with low culture volume

uHighly concentrated, ultrapure pDNA isolation; in very low elution volumes


Unique TechnologyCombines the use of a specially designed filtration devices and tube extender to

obtain high purity pDNA in just 30 minutes. No desalting is required to obtain

ultrapure pDNA yields.



uCloning


uPCR

uLigation

uMicroinjection

uProbe Generation

Ultrapure Plasmid DNA: A / A = 1.8-2.0260 280PurityYield & Binding Capacity

0

300

600

900

1200

1500

mdi Competitor Q

Yield

Binding Capacity

Unique high binding

capacity spin maxi column



QXPK QXPK 10 0010

25 0025

Example: QXPK XX XX XX X 0025

Specifications

Low Copy

Plasmid

High Copy

Plasmid


Recovery


1200 g

80% 80%

130 ml 200 ml

Expected yield of plasmid upto 1000 g upto 250 g

1200 g

(g)


14/34


Express Midi Kit(Less Than 1 Hour)



MDPK MDPK 25 0025

100 0100

Unique Performance AdvantagesuFast pDNA isolation


uHigh pDNA yields and purity

uHighly concentrated yields in very low elution volumes



uCloning


uPCR

uLigation

uMicroinjection

uProbe Generation


Purity

Yield and Binding Capacity

0

100

200

300

400

Elution Volume (ml)

mdi Competitor Q

Yield

Binding Capacity

Example: MDPK XX XX XX X 0100

Specifications

Low Copy

Plasmid

High Copy

Plasmid


Recovery


350 g

80% 80%

50 ml 100 ml


350 g

(g)


15/34

ORDERING

INFORMATION

Specifications

Low Copy

Plasmid

High Copy

Plasmid


Recovery


750 g

80% 80%

150 ml 250 ml


750 g

Express Maxi Kit(Less Than 1 Hour)

Unique Performance AdvantagesuFast pDNA isolation


uHigh pDNA yields and purity

uHighly concentrated yields in low elution volumes



uCloning


uPCR

uLigation

uMicroinjection

uProbe Generation


Purity

Precipitation

Filtration ofCulture Debris

DNA Elution by Vacuum

0

150

300

450

600

750

900

Elution Volume (ml)

Yield & Binding Capacity

Yield

Binding Capacity

mdi Competitor Q



MXPK MXPK 10 0010

25 0025

Example: MXPK XX XX XX X 0025


(g)

Final Elution of Concentrated DNA


16/34


Sample Types PurityAmount of Starting

MaterialYields

Processing

TimeApplications

Mammalian

Tissue

- Liver- Kidney- Lung- Spleen- Mouse Tail

1.8-2.0 25mg 10 -30g < 1hr

?In-Vitro Transcription

?In-Vitro Translation

?Automated Sequencing

?Cloning

?Southern Blotting

?AFLP

?RFLP

?RAPD

?PCR


?Transformation

?Ligation

?Transfection

?SNP Genotyping

?Microsatellite analysis

?RT-PCR

Animal Blood 1.8-2.0 200l Upto 12g


17/34

gDNA Miniprep Kits

mdi Genomic DNA Miniprep Kit mdi Plant gDNA Miniprep Kit

Genomic DNA Isolation Kits: Process Flow


Sample

Bind

Wash 2X

Elute

Ultra Pure gDNA

Dry with open lid

Lyse

Plant Tissue

Bind DNA

Wash 2X

Elute

GrindLysePrecipitate

Centrifuge through

mdi Shredder

Add Binding Buffer andtransfer to mdi plantmini spin column

Ultra Pure gDNA


18/34


gDNA Miniprep Kit( for Mammalian Tissue, Blood, Bacterial Cells and Cultured Cells )

Unique Performance AdvantagesuVery high binding capacity - Upto 50g

uHigher yields

uNo precipitation step for high purity gDNA.

uSuitable for all type of downstream applications

ApplicationsuPCR

uSouthern Blotting

uRAPD Analysis

uAFLP Analysis

uRFLP Analysis

uIn- Vitro Transcription


uTransformation

uTransfection

uSNP Genotyping

uMicrosatellite Analysis

uRT - PCR

uGene Silencing

uMicroinjection

uProbe Generation

Binding capacity of membrane (ds DNA) : 50 g

Recovery : 80%

Minimum elution volume : 200l


Specifications

Purity

Maximum Amount of Tissue :25 mg9

Maximum bacterial cells : 2 x 10Maximum volume of blood sample : 200l

6Maximum amount of cultured cells : 5 x 10

Capacity of column reservoir : 700l

Highly Concentrated Yields

0

10

20

30

40

50

60

mdi Competitor Q

Second Elution

First Elution

Yield(g)

Marke

r

Thermus

therm

ophil

us

Marke

r

Thermus

aquaticus

EcoliD

h5

Agarose gel analysis of gDNA fromdifferent bacteria purified with mdi

gDNA Miniprep Kit . M: lambda-HindIII

Elution Volume: 200l/Elution

ORDERING

INFORMATION



CTGK CTGK 50 0050

250 0250

1000 1000

Example: CTGK XX XX XX X 0250


19/34

ORDERING

INFORMATION

Plant gDNA Miniprep Kit

Unique Performance AdvantagesuVery high binding capacity - Upto 50g

uHigher yields with different plant tissue

uNo precipitation step for high purity gDNA

Suitable for all type of downstream applications

ApplicationsuPCR

uSouthern Blotting

uRAPD Analysis

uAFLP Analysis

uRFLP Analysis

uIn- Vitro Transcription


uTransformation

uTransfection

uSNP Genotyping

uMicrosatellite Analysis

uRT - PCR

uGene Silencing

uMicroinjection

uProbe Generation


Specifications

Purity

Maximum Amount of Starting Material :100 mg wet weight, 20 mg dry weight

Capacity of column Reservoir : 700lBinding capacity of membrane (ds DNA) : 50 g

Recovery : 80%

Elution volume : 100l


Highly Concentrated Yields

0

3

6

9

12

15

mdi Competitor Q

Yield(g)

( Tomato Leaves)

gDNA: Yield

Different Samples

Tomato Potato Spinach Wheat Chickpea

Rice

0

5

10

15

20

25

Yield(g)

Fungus



PTGK PTGK 50 0050

250 0250

1000 1000

Example: PTGK XX XX XX X 1000


20/34

DNA Isolation Kits Purity DNA QuantityRecovered

DNA Fragment

Elution

VolumeApplications

PCR Purification Kit 1.8-2.0 5-10g 100bp -10kb 30l

?Transfection

?In-Vitro Transcription

?

In-Vitro Translation

?High quality sequencing

?Microarray Analysis

?Cloning

?PCR

?Gene Silencing

?Probe Generation

?Microinjection


?Demanding enzymaticmodifications

?Library Construction

?Bacterial Transformation

?Ligation

Micro PCRPurification Kit

1.8-2.0 1-2g 70bp -4kb 10l

Nano PCRPurification Kit

1.8-2.0 upto 400ng 70bp -4kb 5l

Gel Extraction Kit

1.8-2.0

5-10g

70bp -10kb 30l

Micro GelExtraction Kit

1.8-2.0 1-2g 70bp -4kb 10l

Nano GelExtraction Kit

1.8-2.0 upto 400ng 70bp -4kb 5l

PCRPurificati

onKits

GelExtractionKits

Selection Chart

PCR Purification and Gel Extraction Kits


mdi offers PCR Purification Kits for efficient removal of contaminants such as primers,

enzymes and salts and Gel Extraction Kits to purify very small quantities of even large

fragment size DNA from upto 400mg of gel slices.

The following selection chart will help you choose the most suitable kit for yourexperiment.


21/34


PCR Purification Kits

mdi PCR Purification Kit

(5-10g)

mdi Micro PCR Purification Kit

(1-2g)

mdiNano PCR Purification Kit

(upto 400ng)

PCR Reaction

Bind

Wash

Add 5 volumes ofbuffer MPB

Elute

Ultra PureDNA Fragment

Dry

PCR Reaction

Bind

Wash

Add 5 volumes ofbuffer NPB

Elute


Dry

PCR reaction

Bind

Wash

Add 5 volumes ofbuffer PB

Elute


Dry


22/34

Gel Extraction Kits

mdi Gel Extraction Kit

(5-10g)

mdi Micro Gel Extraction Kit

(1-2g)

mdi Nano Gel Extraction Kit

(upto 400ng)

SolubilizedGel Slice

Bind

Wash

Elute

Ultra Pure DNAFragment

Dry


Solubilized

Gel Slice

Bind

Wash

Elute

Ultra Pure DNA

Fragment

Dry

Solubilized

Gel Slice

Bind

Wash

Elute

Ultra Pure DNA

Fragment

Dry


23/34

ORDERING

INFORMATION

PCR Purification Kit(5-10g)

Unique Performance AdvantagesuEasy purification of large sized fragments without shearing

uNo precipitation step for high purity DNA

uHighly concentrated DNA yields in low elution volume

ApplicationsuTransfection

uIn-Vitro Transcription

uIn-Vitro Translation

uHigh quality sequencing

uMicroarray Analysis

uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection


uDemanding enzymatic modifications

uLibrary Construction


uLigation

Total eluate volume : 28 l

Ultrapure Plasmid DNA: A / A =1.8-2.0260 280

Specifications

Purity



Recovery : 90-95%Recovered DNA fragment : (100 bp -10 kb)

Minimum elution volume : 30 l


Complete primer removal using

mdi PCR Purification Kit

a: Before purification b: After purification

M: Marker

1000 bp

200 bp

Primer

M Ma b a b



SPCK SPCK 50 0050

250 0250

1000 1000

Example: SPCK XX XX XX X 0250


24/34

ORDERING

INFORMATION

Micro PCR Purification Kit(1-2g)

Unique Performance Advantages uEasy purification of large sized fragments without shearing


uHighly concentrated DNA yields in very low elution volume






uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection





uLigation

Total eluate volume : 9 l


Specifications

Purity



Recovery : 80%Recovered DNA fragment : (70 bp - 4 kb)




0

20

40

60

80

100

120

mdi Competitor Q

Feed: 1g

%R

eco

very



MPCK MPCK 50 0050

250 0250

1000 1000

Example: MPCK XX XX XX X 0250


25/34

ORDERING

INFORMATION


Nano PCR Purification Kit(upto 400ng))

Unique Performance AdvantagesuWorks with very small DNA quantities

uFastest DNA Purification in just 5 minutes

uHigh DNA Recovery and yield from even low feed quantities

uHighly concentrated DNA in very low elution volumeuEasy purification of large sized fragments without shearing






uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection





uLigation

Total eluate volume : 4l


Specifications

Purity

Capacity of column reservoir : 800lBinding capacity of membrane (ds DNA) : 5 g

Recovery : 80-85%

Recovered DNA fragment : (70 bp-4 kb)

Minimum elution volume : 5l


0

20

40

60

80

100

120

61.6 ng 107.6 ng 422.7 ng

Feed

%R

ecovery

Elution Volume : 5l



NPCK NPCK 50 0050

250 0250

1000 1000

Example: NPCK XX XX XX X 0250


26/34

ORDERING

INFORMATION


Gel Extraction Kit(5-10g)



uHighly concentrated DNA yields in low elution volume






uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection





uLigation



Specifications

Purity


Maximum weight of gel slice : 400mg

Binding capacity of membrane (ds DNA) : 10 gRecovery : 70-80 %

Recovered DNA fragment : (70 bp-10 kb)



SGEK SGEK 50 0050

250 0250

1000 1000

0

2040

60

80

100

50 100 150 200 250 300 350 400

Gel Weight (mg)

Elution Volume (30 l)

Gel weight vs. % Recovery

%R

ecovery

High recovery using mdi Gel Extraction Kit

B: Before extraction 1-2: After extraction

M: Marker

1000 bp

200 bp

M 1 2 B

Example: SGEK XX XX XX X 1000


27/34

ORDERING

INFORMATION


Micro Gel Extraction Kit(1-2g)



MGEK MGEK 50 0050

250 0250

1000 1000



uHighly concentrated DNA yields in very low elution volume






uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection





uLigation



Specifications

Purity


Maximum weight of gel slice : 400mg

Binding capacity of membrane (ds DNA) : 5 gRecovery : 80 %

Recovered DNA fragment : (70bp-4 kb)

Highly Concentrated Recovery

0

15

30

45

60

75

90

mdi Competitor Q

%Re

covery

Feed: 1g

Example: MGEK XX XX XX X 0250


28/34

ORDERING

INFORMATION


Nano Gel Extraction Kit(upto 400ng)

Unique Performance AdvantagesuFastest DNA Purification

uVery high DNA binding capacity

uHigh DNA Recovery and yield from even low feed quantities

uHighly concentrated DNA ; in very low elution volumesuEasy purification of large sized fragments without shearing






uCloning

uPCR

uGene Silencing

uProbe Generation

uMicroinjection





uLigation

Minimum elution volume :

Total eluate volume : 4l


Specifications

Purity

Capacity of column reservoir : 800lBinding capacity of membrane (ds DNA) : 5g

Recovery : 80-85 %

Recovered DNA fragment : (70bp-4 kb)

5l



NGEK NGEK 50 0050

250 0250

1000 1000

Example: NGEK XX XX XX X

0

20

4060

80

100

54.6 ng 128.3 ng 392.8 ng

Feed

%Re

covery

Elution Volume : 5 l

0250


120


29/34

120

105

0

50

100

150

SCN

0.2m

0.45m

2

(g/cm

)

Protein binding capacities of SCN membranes with BSA

mdi binding membranes are uniform, paper thin, white plastic supports, having

specially designed porous structures and binding sites suitable for transfer and

hybridization of biological molecules.

mdi offers a wide range of binding membranes viz. Nitrocellulose, Nylon-66, andPVDF, exhibiting a range of properties to suit various applications.

Special featuresuHigh binding capacities of the transferred molecules

uGood wettabilty for Nitrocellulose and Nylon-66 membranes

uPVDF membranes are hydrophobic

uAbility to retain the molecules without affecting its biological activity

uChemical compatibility and mechanical durability

uAbility to be blocked by simple procedures

uHigh signal to noise ratio

For special applications,mdioffers internally supported binding membranes which

exhibit very high mechanical strength.

Nitrocellulose Membrane Type - SCN and SCNJ

SCN

Pure nitrocellulose membrane produced specially for life sciences applications.

SCNJ

Internally supported to offer superior handleability.

CharacteristicsuHigh binding capacities for proteins and nucleic acid molecules

uMinimum background: High signal to noise ratio

uUniform and easy wettability

uCan be blocked by normal blocking methods

uDoes not bind common protein stains

uCompatible with colorimetric, radiolabelled, chemiluminescent, fluorescent,

and staining detection methods

ApplicationsuProtein blotting

uDot and slot blots

uNucleic acid dot/slot blots

uColony/plaque lifts

uEnzyme immunoassays

PackagingSealed in aluminum bags to maintain hydrophilicity and high protein binding on

prolonged storage. The separators are of pure polyester film to avoid any

contamination of the membrane from the separator.

Binding Membranes for Molecular Biology


Protein Binding


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mdi Polyvinylidene fluoride (PVDF) membrane is a naturally hydrophobic support

matrix which offers much higher protein binding capacities than that of

Nitrocellulose membrane and also binds difficult to bind proteins such as

glycoproteins.

CharacteristicsuVery high binding capacities

uMinimal background: High signal to noise ratio

uRemains flexible and non-brittle after processing

uChemically resistant to harsh reagents making it a convenient matrix for

protein sequencing

uHigher strength than pure nitrocellulose

uCompatible with all types of detection methods

ApplicationsuIdeal support matrix for protein sequencing

uFor high performance, reproducible western blotting

uFor protein staining, glycolipid detection and immunoblotting

Nylon-66 Membrane Type - SNNP and SNNPZ

SNNP: Internally supported Nylon-66 membranes

SNNPZ: Positively charged for enhanced binding of negatively charged molecules

Characteristics

uVery high binding capacities for nucleic acid moleculesuEasy wettabilty

uUltraviolet cross linkable

uChemically resistant; tolerant to alkali fixation

ApplicationsuNucleic acid transfers

uDots/slot blots

uColony/plaque lifts

uMultiple reprobing

PVDF Membrane - Type SVF

240

190

120105

0

50

100

150

200

250

0.45 0.2

2

(g/

cm

)

SVF

SCN

Pore Size ( )m

Protein binding capacities of SVF and SCN membranes with BSA

Protein Binding

ORDERING

INFORMATION

Pore Size XX XX Sterile/ Non Sterile Pack Size

Pore Size Code Code Pack Size Code

0.2m 01 Non Sterile 1 25 11

0.45m 02 50 03

Roll 01

Size

Dia Code

82mm 13

90mm 14

137mm 20

142mm 16

80mm x 100mm 88

150 x 150mm 87

200 x 200mm 86

300 x 300mm 85

3M X 300mm 84

3M x 240mm 833M x 100mm 81

* SVF, SNNP and SNNPZ are available in a maximum width of 240 mm

** SCNJ is available in a maximum width of 100mm

SCNJ 13 02 XX XX 03

Example:

1

Type

Type Code

SCN SCNX

SCNJ SCNJ

SVF SVFX

SNNP SNNP

SNNPZ SNPZ


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SVFSNNPZ

HR

R

R

R

HR

R

R

R

RR

HR

R

R

R

P

P

P

R

R

NR

SNNP

This selection chart highlights the suitability of various mdi membranes for differentapplications based on their properties.

SCNJ

HR

HR

R*

NR

NR

HR

R

R

R

R

RR

P

R

R

R

R

R

R

R

HR = Highly Recommended

R = Recommended

R* = Recommended for Proteins only

P = Possible

NR = Not Recommended

Membrane Type

Colony or Plaque lift

Detection Method

Molecule Fixation

Molecule Removal

Transfer Method

Alkaline Transfer

Electrotransfer

UV Crosslinking

Biomolecules

Nucleic Acids

Capillary Blot

Alkali Fixation

Radiolabelled

Luminesence

Vacuum Blot

Colorimetric

Fluorescence

Reprobing

Proteins

Dot Blot

Staining

Multiple

BakingDrying

Once

Selection Chart

HR

HR

HR

NR

R

R

R

R

R

R

RR

R

R

R

P

P

P

R

R

NR

NR

HR

NR

R

R

R

R

R

R

R

R

R

R

R

R

R

R

R

R

SCN

R

HR

R

HR

R*

R

R

NR

RR

P

NR

HR

R

R

R

R

NR

NR

NR


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ORDERING

INFORMATION


Type Size Pore Size Inlet/Outlet X Bell Sterile/ Non Sterile Pack Size

Type Code Dia Code Pore Size Code Code Size Code Pack Size Code

SYPL SYPL 4mm 01 0.2m 01 Female Luer Lock M With Bell B Non Sterile 1 100 04

SYKG SYKG 13mm 03 0.45m 02 Male Luer Slip N Without Bell X Sterile 2 12 08

IKG IKGX 25mm 06 1/4 SHB* B

Pre-Sterilized Polyethersulfone Membrane Syringe Filters

SYPL-S Pre-sterilized syringe filters are available in pore sizes of 0.2m and 0.45m

and several diameters to suit various applications for filtration in the laboratory.

Special Types Available

SY25KG-S

This special syringe filter houses a microglassfiber pre-filter along with the

Polyethersulfone membrane filter and is ideal for filtering difficult to filter solutions.

IKG-S

This 50mm Polyethersulfone syringe filter with 1/4 stepped hose barb connections

incorporates large filtration area and microglassfiber pre-filter for larger volume

filtration. These filters can be used with 50ml syringe to filter volumes up to 500ml.

Special FeatureExtremely low protein binding exhibited by mdi syringe filters results in very high

product recovery.

99.9% 98%

0

50

100

SYPL Competition

BSA

Note: only IKG-S filters are available with bell

Example: SYKG 06 01 MN X X 2 04

Specifications

Pore Size 0.2m, 0.45m

Diameter 4mm

EFA* 0.07cm

Hold-Up Volume 7 for Br. diminuta

0.45m: LRV >7 for Sr. marcescens

13mm

0.8cm


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ORDERING

INFORMATION


Vacufil-S: Pre-Sterilized Bottle Top Vacuum Filters

mdi Pre-Sterilized Bottle Top Vacuum Filtration units with an extra large 75mm

diameter, low protein binding Polyethersulfone membrane are the best option for

filtration of biologicals like sera and culture media, and other proteinaceous solutions.

Vacufil filters have a hydrophobic filter in the vacuum arm to prevent passage offiltrate to the pump. These filters screw perfectly on to vacuum safe bottles with

45mm neck size.

FeaturesuLow protein binding

uExtra large filter area

uHigh flow rates

u100% Integrity tested

uNo elastomers or adhesive used in sealing

uNon-toxic materials of construction

0.2m, 0.45m

75mm

45mm (Screw cap neck)

7 for Br. diminuta

SpecificationsPore Size :

Diameter :

Connection :

Hold-Up Volume :

Retention Efficiency :

Type Size Pore Size XX XX Sterile/ Non Sterile Pack Size

Type Code Dia Code Pore Size Code Code Pack Size Code

Vacufil VFPX 75mm 11 0.2m 01 Sterile 2 12 08

0.45m 02 24 12

Example: VFPX 11 01 XX XX 2 12


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