biophotonics ii - kirchhoff institut für physik · kirchhoff-institut für physik internal...

Kirchhoff-Institut

für Physik

InternalProf. Dr. Petrich Biophotonics II (SS 2018)

Biophotonics IIgeneral remarks

BIOPHOTONICS I (WS 2017/18)

I. Imaging Systems• human vision

• microscopy

II. Light Scattering• Mie scattering

• light propagation in tissue

BIOPHOTONICS II (SS 2018)

III. Biospectroscopy• Fluorescence spectroscopy

• Phosphorescence, bio- and

chemiluminescence

• Vibrational spectroscopy

IV. Lasers in medicine• Laser interaction with tissue

• Applications

Literature:

• Bergmann-Schäfer, Optik (Walter de Gruyter)

• E. Hecht, Optik (Addison-Wesley)

• J. Bille, W. Schlegel, Medizinische Physik 3

(Springer)

• P.N. Prasad, Biophotonics (Wiley)

• T. Vo-Dinh, Biomedical Photonics Handbook (CRC

Press)

• V.V Tuchin, Handbook of Optical Biomedical Diagnostics

(SPIE Press)

• G.G. Hammes, Spectroscopy for the biological sciences

(Wiley)

• J.R. Lakowicz, Principles of Fluorescence

Spectroscopy (Springer)

• It is NOT required to have attended the „Biophotonics I“ lecture prior to visiting „Biophotonics II“.

• Lecture „Biophotonics II“ will be credited with 2 CP subject to successfully passing the written exam.

• If you intend to obtain credit points, i.e. to participate in the exam, you will have to register at:

https://uebungen.physik.uni-heidelberg.de/v/892

1


Kirchhoff-Institut

für Physik

Internal

Biophotonics IIgeneral remarks

Klausur : July 23rd, 2018, 9:15-10:45 h

registration: https://uebungen.physik.uni-heidelberg.de/v/892

Please bring your own, completely empty (!) white paper sheets

Allowed:

- 1 DIN A4 page with hand-written information such as formulas etc.

(both sides are o.k.)

- Pencil, etc., simple pocket calculator (not on smart phone, ipad and such !)

- Brain

Not allowed:- Internet connection of any kind

- Smart phones, cell phone, ipads etc., notebooks, netbooks etc.

- Your neighbor‘s solutions

Personal advice: 1.) be on time

2.) carefully read the question, then think, then write

Prof. Dr. Petrich Biophotonics II (SS 2018) 2


Kirchhoff-Institut

für Physik

Internal

Biophotonics IIlecture #10 (July 2nd, 2018): summary

IV.4. photothermal interaction

Application examples:

- photothermal therapy (PTT)

esp. with nanoparticles (experimental)

- photocoagulation of retina

(melanin, hemoglobin + heat transfer)

- Diabetic retinopathy- Non-profliferating (leaks): focal laser photocoagulation

- Prolifertaing (neovascularization): panretinal laser photocoagulation

- Age-related macular degeneration (AMD)

T [°C] effect duration laser

> 40 Enzymes damage, oedema, for long

exposure times cell death

> 60 Coagulation, (further) denaturing of

proteins, nectrosis

ms Nd:YAG

> 90 Vaporization, evaporation/boiling of

water („photothermal ablation“)

cw, ms…s Ar+, Kr+, CO2, diodes,

Er:YAG, Ho:YAG

> 150 carbonisation µs…ms

> 300 Melting ( tissue welding) µs…ms

absorption generation of heat(i.c., i.s.c., vibrational relaxation)

heat transport

„heat equation“

- Port wine stain removal

- Laser tissue welding

- Laser hair removal (targets

melanin in follicle)


Kirchhoff-Institut

für Physik

Internal

Biophotonics IIIV. Lasers in medicine

IV.3. photochemical interaction

IV.4. photothermal interaction

IV.5. photoablation

IV.6. photodisruption and

plasmainduced interaction


Kirchhoff-Institut

für Physik

Internal

Biophotonics IIphotoablation


Kirchhoff-Institut

für Physik

Internal


Aus: Bille, Schlegel, „Medizinische Physik“ Band IIIProf. Dr. Petrich Biophotonics II (SS 2018) 6

Kirchhoff-Institut

für Physik

Internal



Kirchhoff-Institut

für Physik

Internal


www.agingeye.net/lasik

cornea

air

aqueous h

um

or

ante

rior

cham

ber

co

rne

al e

pith

eliu

m

Bo

wm

an

‘s laye

r

co

rne

al str

om

a

Desce

me

nt‘s m

em

bra

n

co

rne

al e

nd

oth

eliu

m

http://www.fda.gov/MedicalDevices/ProductsandMedicalProcedures/SurgeryandLifeSupport/LASIK/ucm061421.htm

http://www.eyemoviepedia.com/videos/2396657302/121


Kirchhoff-Institut

für Physik

Internal


Flap Surgery

(LASIK)

Surface Surgery

(LASEK, Epi-LASIK)

Eye Pain after Surgery Minimal

(may last up to 12 hours after

surgery)

Moderate to Severe

(may last up to 72 hours after

surgery)

Functional Vision Recovery Earlier

(Less that 24 hours)

Later

(3 to 7 days)

Stable Refraction Earlier

(1 to 6 weeks)

Later

(3 weeks to months)

Corneal Scarring Risk Minimal

(Less than 1%)

Greater

(1 to 2 %)

Dry Eyes Symptoms More risk

(may last more than 6 months)

Less risk

(lasts for 1 to several weeks)

Risk of Complications More risk

Flap issues: Flap wrinkles,

Epithelial ingrowth, Flap melt

Less risk

In general, safer than LASIK.

Best For Most patients Patients with thin corneas or

large pupils, contact sports

www.agingeye.net/lasikProf. Dr. Petrich Biophotonics II (SS 2018) 9

Kirchhoff-Institut

für Physik

Internal

Biophotonics IIlaser tissue interaction

IV.6. plasmainduced interaction & photodisruption


Kirchhoff-Institut

für Physik

Internal

aus: J. Bille, W. Schlegel, Medizinitechnik, Bd. 3, Springer Verlag

Biophotonics IIplasma-induced laser ablation and photodisruption


Kirchhoff-Institut

für Physik

Internal

Biophotonics IIoptical tweezer

K.C. Neumann et al., Cell, Vol. 115, 437–447, November 14, 2003


biophotonics ii - kirchhoff institut für physik · kirchhoff-institut für physik internal...

Documents