bion octm ii carriers - wakenbtech.co.jp · cell culture matrix bion oc ii manufactured by 100% pet...

BioN OCTM II Carriers

PUREPURELIGHTERLIGHTERHIGHER S/HIGHER S/VVCOST EFFECTIVECOST EFFECTIVE

CESCO Bioengineering Co., Ltd. www.cescobio.com.tw

Cell Culture MatrixCell Culture Matrix

BioNBioNOOC IIC II

Manufactured by 100% PET non-woven fabrics


Features� PURE, 100% pure PET material

� LIGHT WEIGHT, 1 gram of carriers occupy 15 ml space

� High Surface to Volume Ratio , S/V ~ 150 cm -1

� High Capacity , 1 gram carriers provide space for over 1x10 9 cells

to propagate

� Special surface treatment to enhance biocompatibi lity.

Hydrophilicity keeps stable for at least two years.

� Special geometric design to enhance mixing and supp ort load-

bearing capacity in packed-bed.

� Render autoclave, gamma- irradiation, and EO sterili zation

treatment

� Non-pyrogenic, non-cytotoxic


Quality

�100% Pure Material; regulation friendly.

�Raw material passed FDA approval .

� Provide complete resource documentation for .

�Provide Certificate of Quality document for each

purchase


BioNBioNOOC IIC II

120 cm 2/ml300 cm 2/mlHigher S/VHigher S/V

0.8 mg/cm 20.2 mg/cm 2LighterLighter

50%PET/50%PP100% PETPurerPurer

CompetitorBioNOC II

and Cost effectiveand Cost effective ……


BioNBioNOOC IIC II


Geometric Design

�Increase load-bearing capability to support packed-bed structure.

�Increase void fraction and reduce diffusion limitation.

�45o Angle generates microscopic eddy for mixing.

45o


Applications� fit fixed bed cell culture system .

�BelloCellTM culture device

�TideCellTM culture device

�any commercial packed bed culture device


Comparison DataComparison DataCommercial disks and BioNOC II

®

carriers


Test Systems

• BelloCell 500 BioNOC II ® Commercial Disks


Microcarrier Characters


BioNOC II ® Commercial Disks

Material 100% PET PET/PP

Dimension 5 mm X 10 mm strip Disk diameter 6 mm

Specific Surface area 2,400 1,200

Packed volume (ml/g) 15 10

S/V 160 120

Autoclavable OK OK

Gamma irradiation OK

Endotoxin tested (EU/ml) <0.25 < 0.5

Bioburden (CFU/gram) < 1 < 0.36

Cytotoxicity tested serum and serum-free culture test

Yes

Regulation guideline USP <87><88>, ISO10993-5 USP <87><88>, ISO10993-5

Test Conditions (1)

BioNOCII ®

Cell Line：：：：HEK 293

Medium：：：： ααααMEM+10%FBS

Medium volume: 500 ml

Seeding : 1.3××××108 cells

Culture Mode: Fed-Batch

Commercial Disks

Cell Line：：：：HEK 293

Medium：：：： ααααMEM+10%FBS

Medium volume: 500 ml

Seeding : 1.3××××108 cells

Culture Mode: Fed-Batch


Growth Curve total cell No.


-48 -24 0 24 48 72 96 120144168192216240264288312336360384

0.0

5.0x108

1.0x109

1.5x109

2.0x109

2.5x109

3.0x109

3.5x109

4.0x109

Cel

l Pop

ulat

ion

(cel

ls/b

ottle

)

Culture Age (hours)

BioNOC II, 6.5 g FibraCel, 10 g

Growth Curve cell No. per gram carriers


-48 -24 0 24 48 72 96 120144168192216240264288312336360384

0

1x108

2x108

3x108

4x108

5x108

6x108

cell

popu

latio

n pe

r gr

am c

arrie

rs

Culture Time (hours)

BioNOC II FibraCel

BioNOC II provide up to 40% higher cell density compared with Fibra-Cel disks.

Cell Mass Production


Total cells

Cells/gram carrier

0.05.0x108

1.0x109

1.5x109

2.0x109

2.5x109

3.0x109

3.5x109

4.0x109

Fin

al C

ell P

opul

atio

n (c

ells

/bot

tle)

BioNOC II carriers FibraCel disks

0

1x108

2x108

3x108

4x108

5x108

6x108

Cel

l den

sity

(ce

lls/g

car

rers

)

HEK-293 Growth on BioNOC II


249 hrs Culture

bion octm ii carriers - wakenbtech.co.jp · cell culture matrix bion oc ii manufactured by 100% pet...

Documents