biomedical technology aligned document… · web viewa2.1. a2.3. a2.4. a4.5. a6.3 (9-12) (b-5c)...

San Diego County Office of Education For Year: 2013-14 Regional Occupational Program Date Printed: 5/19/2023

Date Reviewed: 10/12/2011 Course Outline/Competencies

Course # - 428807 - Biomedical Technology Approved Hours: 390District - 61 - Poway Unified School District Area: NorthSubmitted by - Malinda Dixon, Amy Peck Date: 11/20/09

Outline Hours Competencies Curriculum StandardsMention – MReinforce – R

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AcademicI. INTRODUCTION TO BIOTECHNOLOGY, PAST AND PRESENT

20 10 10INTRODUCTION TO BIOTECHNOLOGY, PAST AND PRESENT - The Student:

A. Setting up a Legal Scientific Notebook

B. Safety in the Biotech Laboratory

C. Internet/WWW Research, Word®, Excel®, PowerPoint®, Project®

D. Scientific Methodology Laboratory

A. Describes major historic developments in biotechnology fields such as pharmaceuticals, agriculture, diagnostics, industrial products, instrumentation and research and development.

B. Identifies the major scientific discoveries that lead to recombinant DNA technology, including those in chemistry, genetics, microbiology, and fermentation technology, and explain how those discoveries are used in industry today.

C. Outlines the steps in production and delivery of a product made through recombinant DNA technology.

D. Uses the scientific method to conduct a valid experiment, including hypothesis formation, data collection, and data analysis.

E. Develops scientific questions,

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Throughout the course, students will demonstrate their knowledge and skills through:1. Written homework, papers, projects2. Applying theory to laboratory assignments3. Contributions to discussion and collaborative learning projects4. Performing Laboratory skills, analyzing data5. Exams (written, oral, and practical)

Student work will be

CODES: B-Biology/Life Sciences, C-Chemistry, IE-Investigation & Experimentation, HSMT/BRD-Health Science & Medical Technology/Biotechnology Research & Development, AF-Algebra & Functions





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Academichypotheses, and experimental plans.

F. Creates data tables and graphs using Excel® for the purpose of collecting and analyzing data.

G. Uses Microsoft Project software to plan and organize projects.

H. Interprets and critically analyzes quantitative and qualitative data.

I. Composes a thorough concluding statement outlining the results of an experiment with evidence, explanations, error analysis, and practical applications.

J. Organizes and communicates scientific findings both orally and in written form and produce clear, concise written and oral reports.

K. Evaluates the validity of results obtained during experimentation and product development. Evaluate scientific reports with well-supported, clearly presented opinions.

L. Uses the Internet and World Wide Web to collect and share scientific

evaluated using criteria aligned to State, district, and site standards.






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Academicinformation.

M. Uses a variety of methods including literature searches, in libraries, in computer databases, and on-line, for gathering background information, making observations, and collecting and organizing data.

N. Works effectively individually and within a team.

II. THE CHARACTERISTICS OF COMMON ORGANISMS USED IN BIOTECHNOLOGY

20 20 20THE CHARACTERISTICS OF COMMON ORGANISMS USED IN BIOTECHNOLOGY - The Student:

A. Model Organism Growth/Media Preparation

B. Sterile Technique/Cell Culture

A. Distinguishes between prokaryotic cells, eukaryotic cells, and viruses.

B. Outlines the life cycle and characteristics of model organisms used in the biotechnology industry, including various bacteria (E. coli) and fungi (yeasts and Aspergillus.

C. Uses various methods to monitor the growth of cell cultures.

D. Describes conditions that promote cell growth under aseptic conditions in the laboratory and workplace.

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AcademicE. Explains how environmental factors

affect the growth of model organisms in the laboratory.

F. Lists and describes the structure and function of cellular organelles.

G. Discusses the structure and function of the macromolecules that compose cells, including carbohydrates, lipids, DNA, RNA, and protein molecules.

H. Conducts indicator tests (Benedict’s, Iodine, Biuret) for the common macromolecules of the cell.

I. Explains the basic concepts of cell growth and reproduction, DNA replication, mitosis, meiosis, and protein synthesis.

III. STANDARD LABORATORY OPERATING PROCEDURES

30 10 10STANDARD LABORATORY OPERATING PROCEDURES - The Student:

A. Solution and Dilution Preparation

A. Sets up and maintains a legal scientific notebook that includes an account of all laboratory procedures, data, and reflections.

B. Recognizes laboratory safety

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Academichazards and avoids them. Identifies the location and use of emergency equipment.

C. Properly and safely uses and monitors a variety of scientific equipment, including pH meters, microscopes, spectrophotometers, pipets, micropipets, balances, etc.

D. Measures mass using electronic and analytical balances.

E. Measures volume using graduated cylinders, pipets, and micropipets.

F. Calculates how to prepare solutions based on mass/volume, % mass/volume, and molar concentrations.

G. Prepares solutions of any volume and concentration.

H. Prepares dilutions of concentrated solutions.

I. Outlines the steps in cell culture, sterile technique, and media preparation.

J. Prepares and maintains plate and broth cultures of bacteria.

K. Determines which equipment is

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Academicappropriate to use for a given task and what units of measurement are used. Uses laboratory apparatus, materials, and technology in an appropriate and safe manner.

L. Follows written protocols and oral directions to perform a variety of laboratory and technical tasks.

M. Performs a variety of biological tests and chemical assays, collect data, perform calculations and statistical analysis.

N. Prepares and aliquots samples, reagents and buffers. Performs chemical reactions and purification procedures similar to those used in product development, testing, and manufacture.

O. Performs specimen collection, label samples, and prepare samples for testing. Handles, transports, and stores samples.

IV. DNA STRUCTURE, FUNCTION, ISOLATION AND ANALYSIS

20 10 10DNA STRUCTURE, FUNCTION, ISOLATION AND ANALYSIS - The Student:






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AcademicA. DNA Isolation and Indicator

AnalysisB. Agarose Gel Electrophoresis C. DNA Synthesis and PCR

A. Describes the relationship between nitrogen bases, nucleotides, and nucleic acids.

B. Recognizes nucleotides on a DNA double helix model.

C. Explains how the structure of DNA affects its function.

D. Describes the role of DNA, RNA, and ribosomes in protein synthesis (The Central Dogma).

E. Explains how the structure of DNA affects its isolation from cells and solutions.

F. Isolates genomic DNA from cells and analyze its purity and concentration.

G. Isolates plasmid DNA from cells (mini-preparation) and analyzes its purity and concentration.

H. Explains the principles involved in agarose gel electrophoresis.

I. Prepares, loads, runs, visualizes, and analyzes DNA samples on an agarose gel.

J. Describes the differences in samples of eukaryotic and

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Academicprokaryotic DNA samples on a gel.

V. PROTEIN STRUCTURE, FUNCTION, ISOLATION AND ANALYSIS

20 10 10PROTEIN STRUCTURE, FUNCTION, ISOLATION AND ANALYSIS - The Student:

A. Protein Isolation and Indicator Analysis

B. Polyacyrlamide Gel Electrophoresis

C. Protein and Enzyme Studies/Assays

A. Identifies eight groups of protein based on their functions, citing specific examples of proteins in each group.

B. Explains the relationship between amino acids, peptides and proteins.

C. Describes primary, secondary, tertiary, and quaternary structure in proteins.

D. Uses the Internet to find information about the structure and function of specific proteins.

E. Prepares protein solutions and dilutions at specific concentrations and pH.

F. Uses protein indicator solutions to identify the presence and concentration of protein in solution.

G. Explains the principles involved in polyacrylamide gel electrophoresis.

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AcademicH. Prepares, loads, runs, visualizes,

and analyzes protein samples on a polyacrylamide gel.

I. Describes the meaning in differences in peptide band seen on polyacrylamide gels.

J. Explains the function of enzymes and how their activity is affected by temperature and pH.

K. Performs enzyme activity assays.VI. THE PRODUCTS AND APPLICATIONS OF MODERN BIOTECHNOLOGY

10 10 10THE PRODUCTS AND APPLICATIONS OF MODERN BIOTECHNOLOGY - The Student:

A. Current uses of biotechnological products.

B. Companies involved in the industry.

C. Sources for components in products.

D. Molecular profilingE. Imaging techniquesF. Appropriate legal

documentation of results

A. Compares and contrasts pure and applied scientific research in the field of biotechnology.

B. Identifies several local biotechnology companies specializing in the production of pharmaceuticals, agricultural products, industrial products, and research instruments and reagents.

C. Describes the major steps is a product’s move through a

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Academiccompany’s product pipeline.

D. Explains how companies decide on the research and development targets and potential products.

E. Identifies several products obtained through recombinant DNA technology.

F. Cites examples of plant parts or extracts used as pharmaceuticals.

G. Uses the Internet to find information about herbal remedies, traditional pharmaceuticals, and recombinant pharmaceuticals.

H. Produces and tests plant extracts for anti-microbial activity.

I. Collects and tests native bacteria for amylase production.

VII. ASSAYS AND ASSAY DEVELOPMENT 30 10 10ASSAYS AND ASSAY DEVELOPMENT - The Student:

A. Amylase Assay DevelopmentB. Spectrophotometry to Study

MoleculesC. Protein Concentration

Assays

A. Designs an assay that shows the presence and activity of an enzyme.

B. Compares and contrasts the use of different assays used in research and production of protein products.

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AcademicC. Explains how Benedict’s Solution

and Lugol’s Iodine are used in glucose and starch testing.

D. Describes how assays for reactants or products can indicate the presence or activity of an enzyme.

E. Illustrates how an ELISA assay works, the role of antibodies in an ELISA, and how it may be used in industry.

F. Conducts an ELISA assay to test for the presence of a specific protein.

G. Identifies the common parts found on visible spectrophotometers and describe their function.

H. Elucidates the relationship between wavelength and the color of light.

I. Cites the colors of different wavelengths of light.

J. Outlines the steps of using a visible spectrophotometer.

K. Describes the relationship between light transmittance and light absorbance in a sample.

L. Uses a visible spectrophotometer to produce absorbance spectra.

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AcademicM. Discusses the difference between

acids, bases, and neutral solutions. N. Uses pH paper and pH meters to

measure and adjust pH. O. Defines the function of a buffer and

give examples of buffers used in a biotechnology lab.

P. Makes several buffers at various volumes, concentrations, and pH.

Q. Describes how pH affects protein structure and function.

R. Prepares a serial dilution of protein and measure their absorbance at a given wavelength.

S. Uses a standard curve to determine the concentration of an unknown protein solution.

T. Using Excel®, does a linear regression to calculate protein concentration.

U. Uses statistical analysis including the standard deviation, to determine the validity of data.

VIII. RECOMBINANT DNA AND GENETIC ENGINEERING

10RECOMBINANT DNA AND GENETIC






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ENGINEERING - The Student:

A. Recombinant Plasmid /Cloning Vectors

B. Restriction Digestion and Restriction Enzyme Mapping

C. Agarose Gel Electrophoresis Analysis

D. Cell CompetencyE. Bacterial Transformation

and Selection

A. Discusses methods to isolate DNA and specific genes for engineering purposes.

B. Enumerates the activities and uses of restriction enzymes.

C. Conducts a restriction digestion of a plasmid.

D. Lists the steps in the production of a recombinant DNA molecule.

E. Cites examples of vectors used in transformation, transduction, and transfection.

F. Describes the steps in a bacterial transformation including competency, recovery, and selection.

G. Conducts a bacterial transformation and select for transformants.

H. Describes methods by which transformants may be selected including antibiotic resistance, GFP and GUS activity.

I. Conducts a mini-prep to retrieve

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Academicplasmids from transformed cells.

IX. BRINGING THE PRODUCTS OF BIOTECHNOLOGY TO MARKET

20BRINGING THE PRODUCTS OF BIOTECHNOLOGY TO MARKET - The Student:

A. Cell Culture, Growth and Monitoring

B. Protein Product Purification and Testing

C. Product Pipelines and Industry Applications

D. Disease and MedicineE. Clinical Testing and

RegulationsF. Meeting management and

Web conferencingG. Communication skills and

public speakingH. Organizing data and

presenting to a groupI. Biotech decision-making

considerations and marketingJ. Financing and due diligence

A. Outlines the steps in product production, recovery, and purification.

B. Describes the characteristics of proteins that allow for their purification after cloning transformed cells.

C. Compares and contrasts the processes of paper, thin-layer, and column chromatography.

D. Explains how PAGE is used with column chromatography to monitor protein product.

E. Describes the steps in harvesting protein product from fermentation cell culture.

F. Tests for the presence and concentration of proteins in processed samples.

G. Cites the steps in buffer exchange

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Academicand dialysis as used in protein processing.

H. Compares and contrasts the mechanism of gel filtration, ion exchange and affinity chromatography.

I. Conducts an ion exchange chromatography to isolate proteins of different charge.

J. Explains the function and use of FPLC and HPLC in research and production.

K. Confirms the results of a column chromatography using spectrophotometry and PAGE.

L. Summarizes the steps in clinical testing and FDA approval for new drugs produced through genetic engineering.

M. Inspects and verifies inventory and integrity of products.

N. Discusses techniques of product packaging and distribution.

O. Records and reports protocols, procedures, results, conclusions, manuals, reports and write memos






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Academicand letters utilizing computer -processing.

P. Interacts with colleagues and supervisors and coordinate tasks.

X. BIOETHICS, COMMUNICATION AND DECISION MAKING IN THE BIOTECHNOLOGY INDUSTRY

10BIOETHICS, COMMUNICATION AND DECISION MAKING IN THE BIOTECHNOLOGY INDUSTRY - The Student:

A. Bioethics and Decision-Making

A. Cites specific examples of how and where biotechnology is used in medical, agricultural, environmental, and industrial applications as well as social or political situations, including criminal investigations, lawsuits, evolutionary studies, etc.

B. Illustrates examples of how biotechnology has lead to benefits and risks to society and how biotechnical advances affects human lives on a personal level.

C. Identifies the rights, interests, and responsibilities of people involved in bioethical issues.

D. Describes the need for and function

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Academicof regulatory agencies such as those in government, industry, and society.

E. Analyzes policy-making procedures for products and techniques of biotechnology.

G. Formulates opinions about engineered organisms and products based on current scientific evidence.

H. Disclaimer: See Poway Unified School District Board Policy 3.11 on teaching controversial issues in the classroom.

XI. CAREERS IN BIOTECHNOLOGY 10CAREERS IN BIOTECHNOLOGY - The Student:

A. Explore careers in the biotechnology industry.

A. Elaborates the opportunities for careers in biotechnology in health, medicine, genetics, agriculture, etc.

B. Presents arguments for pursuing careers in biotechnology at differing entry-levels.

C. Developed a portfolio that demonstrates proficiency in specific tasks including writing samples and

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Academicperformance-based skills.

D. Creates an appropriate resume for use in applying for laboratory positions at a biotechnology company.

E. Demonstrates knowledge of the vast variety of departments and positions, scientific and nonscientific, at a typical biotechnology company.

XII. EXPECTED STUDENT LEARNING RESULTS1 OCCUPATIONAL KNOWLEDGE AND SKILLS (infused throughout course)

10 10 10 EXPECTED STUDENT LEARNING RESULTS

A. OCCUPATIONAL KNOWLEDGE AND SKILLS:The students has demonstrated:

1 Accessing and utilizing technology and information2 Practicing occupational safety standards3 Thinking critically and solving problems effectively4 Using basic skills in reading, writing, mathematics, listening and speaking as they relate to occupation specific skills5 Attaining a comprehensive






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Academicunderstanding of all aspects of industry the individual is preparing to enter6 Applying knowledge to real world problems and situations

2 WORKPLACE SKILLS AND BEHAVIOR (infused throughout course)

B WORKPLACE SKILLS AND BEHAVIOR:

The student has demonstrated the following standards of professionalism:1 Works independently and collaboratively.2 Communicates effectively and appropriately.3 Performs reliably and responsibly.4 Respects diversity.5 Manifests dependability and punctuality. 6 Complies with job requisites of safety, neatness, and follows direction well.7 Works well with others and gives positive responses to criticism.






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Academic8 Works beyond minimum job expectations.

3 JOB ACQUISITION SKILLS/LIFELONG LEARNING OPPORTUNITIES

C JOB ACQUISITION SKILLS/LIFELONG LEARNING OPPORTUNITIES:

The student has demonstrated an awareness of career options and lifelong learning opportunities by:1 Completed an appropriate resume and job application.2 Modeled job interview techniques.3 Attained awareness of advanced career and educational opportunities and the need for continuous education.

D OUTSTANDING STUDENT AWARD1 The student has received the ROP Outstanding Student Award.

Total Hours 210 90 90


biomedical technology aligned document… · web viewa2.1. a2.3. a2.4. a4.5. a6.3 (9-12) (b-5c)...

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