biochemistry of blood elements vladimíra kvasnicová the figure is found at 20cells.htm (march...
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Biochemistry of Blood Elements
Vladimíra Kvasnicová
The figure is found at http://www.biosbcc.net/doohan/sample/htm/Blood%20cells.htm (March 2007)
Blood Elements Count
erythrocytes 4 - 6 x 106 / l
leukocytes 4 - 9 x 103 / l
neutrophils 47 - 75 %eosinophils 1 - 4 %basophils 0 - 1 %lymphocytes 23 - 45 %monocytes 2 - 11 %
thrombocytes 150 - 400 x 103 / l
Red Blood Cells(erythrocytes)
The figure is found at http://www.vghtpe.gov.tw/~hema/hematopoitic%20cell%20differentiation/RBC.htm (March 2007)
Red Blood Cells(erythrocytes)
1. Function erythrocyte as a bag for hemoglobin
O2 → transport, reactive oxygen species (ROS)
CO2 → transport, formation of HCO3-
H+ → transport, maintaining pH(35% of blood buffering capacity)
Red Blood Cells(erythrocytes)
2. Structure large surface
(diffusion of gases)
cytoskeletal proteins (elasticity)
membrane as an osmometer
(Na+/K+-ATPase)
The figure is found at http://www.biosbcc.net/doohan/sample/htm/Blood%20cells.htm (March 2007)
The figure is found at http://www.vet.purdue.edu/depts/bms/nour/bms520/content/blood/b9.htm (March 2007)
• hemolysis(blood,
plasma)
What happens to red blood cells when placed in hypotonic, hypertonic, and
isotonic solutions?
• osmolarity(285 mosmol/l)
• acanthocytes
The figures are found at http://www.wadsworth.org/chemheme/heme/microscope/pix/spherocytes_nw.jpg and http
://www.mie.utoronto.ca/labs/lcdlab/biopic/fig/4.23b.jpg (March 2007)
Red Blood Cells(erythrocytes)
membrane and cytoskeletal
proteins
hereditary spherocytos
is
Red Blood Cells(erythrocytes)
3. membrane transporters Na+/K+-ATPase (active transport)
GLUT-1 (insulin independent)
anion exchanger = band 3 protein (Cl-/HCO3-)
4. membrane antigens blood groups
The figure is found at http://www.life.umd.edu/classroom/bsci422/mosser/ABO.gif (March 2007)
ABO system
Red Blood Cells(erythrocytes)
5. metabolism glucose is the main fuel
90% anaerobic glycolysis(ATP, lactate: Cori cycle; 2,3-BPG)
10% hexose monophosphate pathway(NADPH)
enzyme defects : * glucose-6-P dehydrogenase
* pyruvate kinase
→ hemolytic anemia
Red Blood Cells(erythrocytes)
5. other important enzymes carbonate dehydratase (= carbonic
anhydrase, CA)
methemoglobin reductase
superoxide dismutase
catalase antioxidative system
glutathione peroxidase
glutathione reductase
Red Blood Cells(erythrocytes)
superoxide dismutase (SOD)
O2• + O2
• + 2 H+ H2O2 + O2
catalase (CAT)
H2O2 + H2O2 2 H2O + O2
Red Blood Cells(erythrocytes)
glutathione peroxidase (GPx)
2 GSH + H2O2 GS-SG + 2 H2O
2 GSH + R-O-OH GS-SG + H2O + ROH
The figure is found at http://www.cs.stedwards.edu/chem/Chemistry/CHEM47/ResMethods2003/graphics/?M=A
(March 2007)
glutathione = „redox buffer“
The figure is found at http://www.med.unibs.it/~marchesi/ppp.html (March 2007)
Hexose Monophosphate
Pathway
glutathione reductase
GS-SG + NADPH+H+
2 GSH + NADP+
The figure is found at http://www.vrp.com/newsimages/march04Fig1.jpg (March 2007)
2
Glutathione system
NADPH+H+
The figure is found at http://www.biosbcc.net/doohan/sample/htm/Blood%20cells.htm (March 2007)
Red Blood Cells(erythrocytes)
6. Erythropoesis
The figure is found at http://faculty.ccp.edu/dept/biol/All_five_leukocytes.jpg (March 2007)
White Blood Cells
(leukocytes)
White Blood Cells(leukocytes)
Classification• granulocytes
neutrophils (phagocytosis)eosinophils (alergy, parasites)basophils (alergy)
• agranulocytesmonocytes → macrophages lymphocytes (B, T)
Reactive oxygen and nitrogen species
in blood elements
ERYTHROCYTESenzymes for deactivation of ROS formed
from high content of oxygen found in the cells
PHAGOCYTESenzymes for production of ROS and
RNS to destroy particles in phagosomes
White Blood Cells(leukocytes)
Neutrophils (microphages)
• high content of lysosoms (hydrolytic enzymes)
• few mitochondria
• glucose dependent: NADPH production
• NADPH is used for production of reactive oxygen species → they kill bacteria
• after activation: RESPIRATORY BURST= increased consumption of O2 and glucose
The figure is found at http://courses.washington.edu/conj/bloodcells/radicals.gif (March 2007)
Reactive oxygen species (ROS)produced by neutrophils
ClO-
White Blood Cells(leukocytes)
The figure is found at http://www.pigur.co.il/imgpku/no_synthesis.gif (March 2007)
NO· is a reactive nitrogen species used by phagocytes
iNOS
White Blood Cells(leukocytes)
proteolytic enzymes /elastase
collagenase
gelatinase
kathepsin G
protease inhibitors
1-protease inhibitor(= 1-antitrypsin)
1-antichymotrypsin
2-macroglobulin
produced by phagocytes = plasma proteins
Their ratio affects an extent of inflammation!
The figures are found at http://life.nthu.edu.tw/~lslpc/BioPhyTalk/heparin01.jpg and http://www.nottingham.ac.uk/~mqzwww/images/histamine.gif (March 2007)
Basofils
contain heparin and histamine
The figure is found at http://www-immuno.path.cam.ac.uk/~immuno/part1/lec06/ab12.gif (March 2007)
B-lymphocytes
produce antibodies (= immunoglobulins, -globulins)
( or )
(, , , , )
The figure is found at http://image.bloodline.net/stories/storyReader$590 (March 2007)
Platelets(thrombocytes
)
The figure is found at http://www.biosbcc.net/doohan/sample/htm/Hemostasis.htm (March 2007)
Platelets(thrombocytes)
participate in hemostasis
• adhesion: glycosaminoglycans and glycoproteins (hyalomera)
• activation: free collagen, thrombin, ADP, TxA2, serotonin• contraction of thrombus: Ca2+, glycogen, ATP