biochem customized lab manual finals
TRANSCRIPT
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 1/36
Ahrlslr| 0A
Hmazahr`zl{arlie ic Nltlbx
L IJK@HRLS@X
Ri `epo`zar` rm` blcc`z`er tm|xlhan tzit`zrl`x ic nltlbx
Ri blxhpxx rm` tzlehltn` j`mleb `ahm r`xr
Ri eao` rm` hiotie`erx ic nltlbx
LL XHM@OARLH BLAGZAO
LLL Z@XPNRX AEB IJX@ZSARLIE
Xinpjlnlr| R`xr
Xins`er @}t`hr`b Z`xpnr @}t`zlo`eran Z`xpnr Ijx`zsarlie
War`z Lexinpjn` Lexinpjn` Cizoarlie ic na|`z.
jlg jpjjn`x
Blnpr` MHN Lexinpjn` Lexinpjn` Cizoarlie ic na|`z.
xoann`z jpjjn`x
Blnpr` EaIM Lexinpjn` Lexinpjn` Cizoarlie ic na|`z.
iln az` xinlblcl`b
@rm|n anhimin Lexinpjn` lexinpjn` Iln lx cipeb ar rm`
jirrio ic rm` rpj`
Hmnizicizo Xinpjn` Xinpjn` blxxins`b
@rm`z Xinpjn` xinpjn` blxxins`b
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 2/36
Z`ahrlie ic carx4 olepr` Acr`z 8 mipzx z`xpnr
Z`b nlropx Z`b/z`b Z`b/z`b Ei z`ahrlie
Jnp` nlropx Jnp`/jnp` Jnp`/jnp` Ei z`ahrlie
LS AEAN\XLX AEB HIEHNPXLIE
Gplb` qp`xrliex;
1, @}tnale rm` xinpjlnlr| ic rm` blcc`z`er flebx ic nltlbx le `ahm xins`er px`b
Nltlbx az` eie/tinaz izgaelh Rm` tm|xlhan tzit`zrl`x ic rm` carr| ahlbx. aeb ic hiotipebx rmar
hierale rm`o. az` nazg`n| b`r`zole`b j| rm` n`egrm aeb b`gz`` ic pexarpzarlie ic rm` m|bzihazjie
hmale Rm` eietinaz m|bzihazjie hmale ahhiperx ciz rm` tiiz xinpjlnlr| ic carr| ahlbx le war`zXinpjlnlr| ic a xpjxraeh` b`t`ebx ie a xlotn` zpn` ic a rmpoj rmar *nlf` blxxins`x rmlx xrar`o`er
leblhar`x rmar a xinpr` wlnn blxxins` j`xr le a xins`er rmar max a xlolnaz hm`olhan xrzphrpz` ri lrx`nc
Rm` is`zann xinsarlie hatahlr| ic a xins`er b`t`ebx tzloazln| ie lrx tinazlr|
War`z/Hihiepr iln lx lexinpjn` le war`z `s`e ar ziio r`ot`zarpz` xleh` war`z lx a tinaz
xins`er lr wlnn eir blxxins`b hihiepr iln wmlhm lx eie/tinaz
Blnpr` MHn/ hihiepr iln lx lexinpjn` le blnpr` ahlb -blnpr` MHn, xleh` MHN lx a eietinaz
leizgaelh xins`er Hihiepr iln max mlgm`z oin`hpnaz w`lgmr aeb wm`e blxxins`b le blnpr`
ahlb r`ebx ri cniar
Blnpr` EaIM/hihiepr iln lx lexinpjn` le blnpr` anfanl -EaIM, Le rm` xinprlie rm` hihiepr iln lr
r`ebx ri cizo ax a wmlr` hinnilban oarr`z acr`z rm` abblrlie ic blnpr` anfanl
@rm|n Anhimin/ Nlf` war`z `rm|n anhimin lx a tinaz xins`er aeb wlnn eir blxxins` oixr
eietinaz xinpr`x xphm ax iln
Hmnizicizo / Hihiepr iln lx xinpjn` le hmnizicizo Iln lx ae tinaz izgaelh hiotipeb xao` lx
rm` tixlrlie wlrm rm` hmnizicizo
@rm`z/ hihiepr iln lx xinpjn` le `rm`z xleh` `rm`z lx ae izgaelh eie/ tinaz xins`er
Cizoarlie ic Rzaexnph`er xtir
Iln px`b Tz`x`eh` ic
rzaexnph`er xtir
Rlo` Bpzarlie
Hihiepr iln & 13 olepr`x
Nlex``b iln & 1 mipz aeb 1
olepr`T`aepr iln
&
2 olepr`x
Hib nls`z iln & :1 olepr`x
Inls` iln & 8< olepr`x
Hize iln & :4 olepr`x
Ahzin`le Cizoarlie
Iln px`b @}t`hr`b z`xpnr @}t`zlo`eran
z`xpnr)ijx`zsarlie
Hihiepr iln Cizoarlie ic ahzin`le6 ahzlb
ibiz
Ahzlb ibiz -&,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 3/36
: @}tnale wm| irm`z ilnx `satizar` caxr`z. wmln` irm`z xmiw xniw `satizarlie Gls` rm`
xrzphrpzan cizopna ic rm` blcc`z`er r|t`x ic iln
@ahm nltlb lx blcc`z`er wlrm `ahm irm`z. `xt`hlann| wlrm rm` epoj`z aeb rm` jzaehmleg ic
rm`lz hazjie hmale. rm` nieg`z rm` hmale aeb rm` oiz` lr lx jzaehmleg rm` nieg`z rm` rlo` lr
`satizar`x Rm`z` lx anxi a cahriz le rm` b`gz`` ic pexarpzarlie ic rm` nltlb Xarpzar`b carr|
ahlbx iz xarpzar`b carx r`eb ri `satizar` a nir nieg`z rmae pexarpzar`b car Xarpzar`b carx az`
hiotix`b ic oae| xlegn` hazjie jiebx rmar oaf` rm` xpjxraeh` n`xx sinarln` Pexarpzar`b carx
az` hiotix`b ic ie` iz oiz` bipjn` jiebx Rm` bipjn` jiebx oaf` rm` car oiz` sinarln` Le
irm`z wizbx rm`z` az` blcc`z`eh`x le rm` `satizarlie ic blcc`z`er fleb ic iln j`hapx` rm`lz
`satizarlie zar` oalen| b`t`ebx ptie rm`lz xrzphrpzan cizopna Rm` blcc`z`eh` le oin`hpnaz
w`lgmr aeb epoj`z ic bipjn` jiebx rm`z`ciz` acc`hr rm` zar` ic `satizarlie ic iln
Hihiepr iln
Hize Iln
Nlex``b iln Inls` iln
T`aepr iln
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 4/36
8 Xmiw hm`olhan z`ahrlie ic Ahzin`le r`xr
Rm` tzlehltn` j`mleb rm` ahzin`le r`xr lx a xt`hlclh hm`olhan z`ahrlie Rmlx z`ahrlie lx prlnl{`b
ri b`r`zole` rm` tz`x`eh` ic gn|h`zle le a car J| m`arleg rm` car xaotn` le rm` tz`x`eh` ic
tiraxxlpo jlxpncar` -FMXI2,. wmlhm ahrx ax a b`m|bzarleg ag`er. ahzin`le -H8M2I. iz HM:?HM/HMI,
lx cizo`b aeb hae `axln| j` b`r`hr`b j| lrx ibiz Wm`e`s`z car lx m`ar`b le rm` tz`x`eh` ic a
b`m|bzarleg ag`er. rm` car oin`hpn` wlnn xm`b lrx gn|h`zin le rm` cizo ic rm` pexarpzar`b anb`m|b` /
ahzin`le
Aean|xlx
Xinpjlnlr|
Nltlbx az` lexinpjn` le eie/tinaz xins`erx nlf` hmnizicizo aeb `rm`z aeb lexinpjn` tinaz xphm
ax war`z. anhimin. ahlb aeb jax` Rmpx. lr cinniwx rm` zpn` ic a rmpoj nlf` blxxins`x nlf`
War`z `s`e hann`b a pels`zxan xins`er wlnn eir blxxins`b rm` hihiepr iln j`hapx` ic lrx eie/
tinazlr| Le rm` r`xr |ip hae x`` rm` cizoarlie na|`z aeb jlg jpjjn`x ic iln ie rm` rit xpzcah`
leblhar`x rmar iln mas` niw`z xt`hlclh gzaslr| rmpx lr cniar ie war`z
Le rm` :eb
rpj` rmar hierale blnpr` MHn wmlhm lx ae leizgaelh ahlb aeb a tinaz xins`er Le rm`
`}t`zlo`er lr xmiwx cizoarlie ic na|`z jpr xoann`z jpjjn`x hiotaz`b ri rm` war`z/iln ol}rpz` Rmlx
leblhar`x rmar MHN xio`miw blxxins`b rm` iln leri xoann`z tazrlhn`x aeb iln max a niw`z xt`hlclh
gzaslr| rmar bln MHN. rmpx cniar ie rm` ol}rpz`
Le rm` 8zb rpj` wmlhm hierale blnpr` EaIM wmlhm lx ae leizgaelh jax` aeb a tinaz xins`er Le
rm` `}t`zlo`er lr xmiwx cizoarlie ic na|`z aeb rm` iln wax xinlblcl`b Rm` xinlblclharlie ic rm` iln
wax bp` ri rm` hm`olhan tzit`zr| ic nltlb wmlhm lx xatielclharlie. wmlhm rpzex iln leri xinlb
Le rm` 2rm aeb 0rm rpj` wmlhm hieralex hmnizicizo aeb `rm`z z`xt`hrls`n| blxxins`b rm` iln
j`hapx` lr lx a eie/tinaz izgaelh xins`er
Rzaexnph`er xtirJax`b ie ipz r`xr z`xpnrx lr xmiwx rmar t`aepr iln `satizar`b clzxr. cinniw`b j| hihiepr iln.
hize iln. hib nls`z iln. inls` iln aeb naxrn| rm` nlex``b iln Lr lx xrar`b rmar rm` blcc`z`eh` le oin`hpnaz
w`lgmr aeb epoj`z ic bipjn` jiebx acc`hr rm` zar` ic `satizarlie ic iln Rm` nieg`z rm` carr| ah|n
hmale aeb rm` c`w`z rm` bipjn` jiebx. rm` niw`z rm` `satizarlie zar` Le ipz r`xr rm` zar` ic
`satizarlie wax anxi acc`hr`b j| rm` qpaerlr| ic iln bzitt`b le rm` tat`z
Z`ahrlie ic carx
Le rm` r`xr rm`z` wax ei hmaeg` le hiniz ic rm` nlropx tat`z le jirm jnp` aeb z`b.
rm`iz`rlhann| rm` iln xmipnb rpze leri ahlblh xleh` lr wax `}tix`b le ae it`e `eslzieo`er
aeb rmpx peb`zgi zaehlblr| Rm` z`xpnr wax oa|j` bp` ri x`s`zan cahrizx nlf` rm` iln px`b
wax eir cz`xm
Ahzin`le r`xr
Le rm` `}t`zlo`er rm` iln gmls` a tixlrls` z`xpnr xleh` hihiepr iln lx a nltlb aeb lx
hiotix`b ic gn|h`zin
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 5/36
Hiehnpxlie
Nltlbx xmiw oae| tm|xlhan aeb hm`olhan tzit`zrl`x Rm`x` tzit`zrl`x w`z` hn`azn| xmiwe le rm`
z`xpnrx ic rm` r`xr j`leg t`zcizo`b nlf` rm` xinpjlnlr| r`xr. rzaexnph`eh| r`xr aeb z`ahrlie ic carx.
ahzin`le r`xr Nltlbx az` xinpjn` le eie/tinaz xins`erx nlf` hmnizicizo aeb `rm`z aeb lexinpjn` le
tinaz xphm ax war`z. anhimin. ahlb aeb jax` Nltlbx. xt`hlclhann| ilnx xmiwx cizoarlie ic rzaexnph`er
xtir. rm` zar` ic `satizarlie aeb cizoarlie ic rmlx rzaexnph`er xtir saz| j`hapx` ic rm` blcc`z`eh`
ic hm`olhan xrzphrpz` Nltlbx wm`e `}tix`b ri it`e alz iz `eslzieo`er wipnb peb`zgi zaehlblr|
xt`hlclhann| i}lbarlie j`hapx` lr lx `}tix`b ri i}|g`e. wm`z`le lr wlnn j`hio` ahlblh Nltlbx xmiw a
tixlrls` z`ahrlie ri Ahzin`le r`xr xleh` lr lx hiotix`b ic gn|h`zin wmlhm lx rm` oale z`ahraer ri cizo
rm` tzibphr ahzin`le
S BIHPO@ERARLIE
Xinpjlnlr| ic iln -czio n`cr; war`z. blnpr` MHN. blnpr` EaIM. `rm|n anhimin. hmnizicizo. `rm`z,
Cizoarlie ic Rzaexnph`er Xtir -czio n`cr; nlex``b iln. t`aepr iln. hib nls`z iln. inls iln. hize iln.
hihiepr iln,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 6/36
Z`ahrlie Ic carx
Ahzin`le r`xr
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 7/36
AHRLSLR\ 0J
HMAZAHR@ZL[ARLIE IC NLTLBX
L IJK@HRLS@X
Blxhpxx rm` xatielclharlie tzit`zr| ic nltlbx
Ri `epo`zar` rm` tzit`zrl`x ic `opnxlc|leg carx # xatielc|leg nazb
@}tnale rm` tzlehltn` le `ahm r`xr `otni|`b ciz nltlbx
LL XHM@OARLH BLAGZAO
A @opnxlclharlie ic Carx
J Xatielclharlie ic Nazb
Xmaf` a bzit ic hihpepr iln #
Xraeb ciz xio`rlo`
Tnah` 1on M:I # abb 1 bzit 40% EaHI8
# a bzit ic hihiepr iln Xmaf`
Xmaf` a bzit ic hihpepr iln wlrm -1 on
blnpr` anjpole xin“e,
Tnah` 0g nazb le -`zn`eo`|`z cnaxf,
Abb :0on anhiminlh
Wazo -le war`z jarm,
Rzaexc`z xinprlie le satizarleg
blxm -wlrm :on M:4,
Ahlblc| wlrm -0 bzitx blnpr`b MHn,
Z`ois` carr| ahlb
E`przanl{` xin“e wlrm -14 EaHI8,
@}rzahr z`xlbp` wlrm -1on `rm|n anhimin,
Z`ois` anhimin -j| `satizarlie ie war`z
jarm,
@satizar` ri bz|e`xx
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 8/36
LLL% Z@XPNRX AEB IJX@ZSARLIE
Xatielclharlie
Z`xpnrx aeb Ijx`zsarlie; Rm` tzibphr wax hz`ao ri jziwelxm hiniz xpjxraeh` wlrm taxr` nlf`
hiexlxr`eh| Acr`z lr lx z`ois`b czio m`ar lr mazb`e`b leri a tiwb`z| nlf` z`xlbp`
LS% AEAN\XLX AEB HIEHNPXLIE
GPLB@ QP@XRLIEX;
1 @}tnale rm` tzlehltn` lesins`b le jirm xatielclharlie aeb `opnxlclharlie
: Wzlr` hm`olhan `qparlie
A @opnxlclharlie ic Carx
@opnxlclharlie lx a tzih`xx j| wmlhm |ip ol} rwi nlqplbx rmar az` izbleazln| loolxhljn`
@opnxlclharlie ic carx ri ol} rm`o wlrm war`z/jax`b xpjxraeh`x. wmlhm max lotizraer lotnlharliex
ciz hiifleg aeb ciz blg`xrlie Ri `opnxlc| carx. |ip e``b xpjxraeh`x xphm ax jln` xanrx iz irm`z
hiotipebx rmar m`nt hiee`hr rm` car aeb war`z Bzitn`rx ic rm` blxt`zx`b hiotie`er zatlbn|
hian`xh` ri cizo a x`tazar` na|`z @opnxlc|leg ag`er opxr j` tz`x`er ri xrajlnl{` rm` `opnxlie
N`hlrmle le rm` `gg wlnn x`zs` ax `opnxlcl`z
J Xatielclharlie ic Nazb
Xatielclharlie lx rm` m|bzin|xlx ic ae `xr`z peb`z jaxlh hieblrliex ri cizo ae anhimin aeb
rm` xanr ic a hazji}|nlh ahlb Xatielclharlie lx a tzih`xx rmar tzibph`x xiat. pxpann| czio carx aebn|` Xatielclharlie lesins`x o`rannlh anfanl jax` -pxpann| EaIM # FIM, m|bzin|xlx ic rzlgn|h`zlb`x.
wmlhm az` `xr`zx ic carr| ahlbx. ri cizo rm` xiblpo xanr ic a hazji}|nar` Le abblrlie ri xiat. xphm
rzablrliean xatielclharlie tzih`xx`x tzibph`x gn|h`zin *Xatielclajn` xpjxraeh`x* az` rmix` rmar hae
j` hies`zr`b leri xiat Le xatielclharlie tzih`xx. w` px`b anhiminlh tiraxm -FIM blxxins`b le
`rmaein # e`przanl{`b carr| ahlbx le rm` nazb, # EaHI8 ri tzibph` mazb xiat6 rm`e a o`rannlh xanr ic
carr| ahlb lx cizo`b
Carx -rzlgn|h`zlb`x, ptie anfanle` m|bzin|xlx -`lrm`z wlrm FIM iz EaIM , |l`nb gn|h`zin aeb
tiraxxlpo iz xiblpo xanrx ic carr| ahlbx -xiat,
iz
R`xr rpj` Z`xpnrx aeb Ijx`zsarlie
Hihiepr Iln le Blxrlnn`b war`z loolxhljn`
40% Xiblpo Hazjiear` aeb hihiepr iln le
Blxrlnn`b war`z
loolxhljn`
Hihiepr iln le Anjpole Xinprlie olxhljn`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 9/36
Aean|xlx
A @opnxlclharlie
Le rm` `}t`zlo`er. ien| le r`xr rpj` 8 rmar rm` rwi nlqplbx. hihiepr iln aeb anjpole xinprlie
j`hao` olxhljn` Xleh` anjpole hieralex ae `opnxlc|leg ag`er n`hlrmle Rm` z`oaleleg : r`xr rpj`x
blbe“r ol} bp` ri ajx`eh` ic ae `opnxlcl`z%
J Xatielclharlie
Le ipz `}t`zlo`er. rm` iprhio` lx tiwb`z`b bp` ri mlgm attnlharlie ic m`ar. war`z
lx rm`e `satizar`b E`s`zrm`n`xx. w` mab a tixlrls` z`xpnr ie xatielc|leg nazb j`hapx` ic anfanle`
m|bzin|xlx |l`nbleg gn|h`zin aeb tiraxxlpo iz xiblpo xanrx ic carr| ahlbx
Hiehnpxlie
Le hiehnpxlie. carx az` lexinpjn` le war`z. xi rm`| wlnn eir ol} mioig`e`ipxn| le rm`
aqp`ipx xinprliex rmar `}lxr le nlsleg rmlegx Jpr. wlrm `opnxlclharlie. nlrrn` bzitn`rx ic car az`
cizo`b wmlhm BI ol} le war`z. `s`en| `eipgm ar n`axr. wlrmipr hnpotleg iz cniarleg le a oaxx Xi.
rm`| hae j` `cclhl`ern| ois`b azipeb rm` jib| aeb px`b le z`ahrliex
Wmln` le xatielclharlie ic nazb. Lr“x ae `}irm`zolh hm`olhan z`ahrlie rmar ihhpzx
wm`e a aeloan car lx ol}`b wlrm a xrzieg anfanl -Anihiminlh Tiraxm, Rm` tzibphrx ic rm`
z`ahrlie az` rwi; xiat aeb gn|h`zle War`z lx anxi tz`x`er. jpr lr bi`x eir `er`z leri rm`
hm`olhan z`ahrlie Rm` war`z lx ien| a s`mlhn` ciz rm` anfanl. wmlhm lx irm`zwlx` a bz|
tiwb`z Rm`z`ciz`. ien| xatielclajn` xpjxraeh`x az` rmix` rmar hae j` hies`zr`b leri xiat
S BIHPO@ERARLIE
Hihiepr iln wlrm war`z Hihiepr iln. war`z. aeb .0%
xiblpo hazjiear`
Xatielclharlie tzibphr
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 10/36
AHRLSLR\ 0H
QPANLRARLS@ R@XR CIZ HMIN@XR@ZIN
L IJK@HRLS@X
Ri blxhpxx rm` tzlehltn` j`mleb rm` qpanlrarls` r`xrx ciz hmin`xr`zin
Ri `}tnale rm` `cc`hrx ic rm` blcc`z`er z`ag`erx px`b le rm` ahrlslrl`x
Ri blxhpxx rm` tzit`zrl`x ic hmin`xr`zin rmar oaf`x lr z`xtiexls` ri Xanfiwxfl aeb
N`lj`zoaee/Jpzhmazb r`xr
LL XHM@OARLH BLAGZAO
Tz`tazarlie;
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 11/36
Xanfiwxfl r`xr; N`lj`zoaee/Jpzhmazb r`xr;
LLL Z@XPNRX AEB IJX@ZSARLIE
R@XR RM@IZ@RLHAN Z@XPNR @]T@ZLO@ERAN Z@XPNR
Z@XPNR IJX@ZSARLIE
Xanfiwxfl r`xr Hmnizicizo na|`z/ z`b ri
jnp`
Ahlb na|`z/ gz``e
/ Hmnizicizo na|`z/
|`nniw
Ahlb na|`z/ izaeg`
N`lj`zoaee/
Jpzhmazb r`xr
Jnp`/gz``e hiotn`} / Ptt`z na|`z/ jziwe
Niw`z na|`z/ bpnn
izaeg`
LS AEAN\XLX AEB HIEHNPXLIE
GPLB@ QP@XRLIE
Gls` rm` tzlehltn` lesins`b ciz Xanfiwxfl aeb N`lj`zoaee/Jpzhmazb r`xrx
Wm`e hieh`erzar`b xpntmpzlh ahlb lx abb`b ri a hmnizicizo xinprlie ic hmin`xr`zin. rm`
hmnizicizo na|`z xmiwx a z`b ri jnp` hiniz aeb rm` ahlb na|`z xmiwx a gz``e cnpiz`xh`eh` Rmlx
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 12/36
lx rm` tzlehltn` j`mleb Xanfiwxfl r`xr Ie rm` irm`z maeb. rm` ah`rlh aem|bzlb` le rm`
N`lj`zoaee/Jpzhmazb r`xr hae z`ahr wlrm rm` H8 m|bzi}|n gzipt ic hmin`xr`zin aeb z`nar`b
xr`zilb le rm` tz`x`eh` ic a xrzieg ahlb xphm ax hieh`erzar`b xpntmpzlh ahlb rmar w` px`b ri
cizo a jnp`/gz``e hiotn`}
Aean|xlx
Rm` xinprlie aeb rm` tz`hltlrar` |l`nb`b acr`z rm` tz`tazarlie wax rm` x`tazarlie ic
hmin`xr`zin -tz`hltlrar`, aeb rzlgn|h`zlb`x iz e`przan carx -xinprlie,
Xanfiwxfl R`xr lx a r`xr ciz hmin`xr`zin Lr wax xalb rmar rm` z`ahrlie tzlehltn` ic rmlx r`xr
leblhar x rm tz x`eh ic bipjn jieb le ie hmin xr zin zleg lx z xtiexljn ciz lrx ajlnlr| ri cizo
hiniz b tzibphrx le rm tz x eh ic hieh erzar b leizgaelh ahlbx aeb lr xmipnb |l nb a na| zleg ic hinizx
Wm`e hieh`erzar`b xpncpzlh ahlb lx abb`b ri a hmnizicizo xinprlie ic hmin`xr`zin. rm`
hmnizicizo na|`z xmiwx a z`b ri jnp` hiniz aeb rm` ahlb na|`z xmiwx a gz``e cnpiz`xh`eh`
Rm` hieh`erzar`b xpncpzlh ahlb z`ahrx wlrm rm` hmin`xr`zin n`ableg ri z`bblxm hiniz Jpr le
ipz `}t`zlo`er. lr blb eir `eb rmar wa| Ipzx |l`nb`b ae izaeg`/jziwe hiniz W`nn. lr wax
`}t`hr`b xleh` w` blb eir cinniw rm` zlgmr tzih`bpz`. lexr`ab ic m`arleg lr le rm` war`z
jarm. w` m`ar`b lr blz`hrn| le rm` mir tnar`
N`lj`zoaee/Jpzhmazb R`xr lx xrlnn a r`xr ciz hmin`xr`zinx Rm` hiniz rmar lx xpttix`b ri j`
|l`nb`b le rmlx r`xr lx b``t gz``e hiniz Rmlx lx bp` ri rm` m|bzi}|n gzipt ic hmin`xr`zin
wmlhm z`ahrx wlrm rm` z`ag`er wmlhm lx ah`rlh aem|bzlb` aeb xpncpzlh ahlb aeb anxi bp` ri
rm` lehz`axleg hiekpgarlie iz rm` rzaexcizoarlie ic a xpjxraeh` ri a m|bzitmlnlh xrar` Jpr
le ipz `}t`zlo`er. lr `eb`b jabn|% W` blbe“r `s`e xaw a xlge ic gz``elxm hiniz% Rm` z`axie
agale ciz rmlx iprhio` lx rm` lehatajlnlr| ic px xrpb`erx ri haz`cpnn| z`ab aeb cinniw wmar
rm` oaepan xalb. jpr wmar“x bie` lx bie` w` kpxr e``b ri j` haz`cpn e`}r rlo`%
Hiehnpxlie
Acr`z t`zcizoleg rm` `}t`zlo`er. ipz gzipt hiehnpb`b rmar feiwn`bg`. ahhpzah| aeb
haz`cpne`xx az` e`h`xxaz| le hiebphrleg ae `}t`zlo`er Le ipz `}t`zlo`er w` rzl`b
x`tazarleg e`przan carx aeb rzlgn|h`zlb`x. aeb w` blb lr `axln| wlrm rm` m`nt ic xio`
z`ag`erx Jpr lr w`er jab le t`zcizoleg rm` : r`xrx wmlhm az` Xanfiwxfl aeb N`lj`zoaee/
Jpzhmazb r`xrx Lexr`ab ic |l`nbleg rm` zlgmr hinizx lr |l`nb`b a s`z| xrzieg hiniz j`hapx` ic
rm` jpzer tz`hltlrar` w` tzibph`b le lrx tz`tazarlie Ipz `}t`zlo`er oa| j` pexphh`xxcpn
jpr ar n`axr w` n`aze`b xio` rmlegx rmar hipnb j` px`cpn le rm` cprpz`
S BIHPO@ERARLIE
Xanfiwxfl R`xr N`lj`zoaee/Jpzhmazb R`xr
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 13/36
AHRLSLR\ 3A
Hazjim|bzar`x
Qpanlrarls` R`xrx ciz Blcc`z`er R|t`x ic Hazjim|bzar`x
L IJK@HRLS@X
J`hio` caolnlaz wlrm hiooie hazjim|bzar`x
N`aze xlgelclhaer blcc`z`eh`x le rm` hm`olhan tzit`zrl`x ic hazjim|bzar`x
Lb`erlc| rm` hazjim|bzar`x tz`x`er le hiooie ciib tzibphrx
LL XHM@OARLH BLAGZAO
A Oinlxhm“x R`xr
J Aermzie` R`xr
Tnah` 1on ic blxrlnn`b M:I -hierzin,. 1 on ic 0% -gnphix`.
ganahrix`. oanrix`. czphrix`. xphzix`. nahrix`. gn|hig`e #
xrazhm xin“e le `ahm r`xr rpj
Abb 0 bzitx ic Oinlxhm Z`ag`er
Ol} hier`er j| g`ern| xmafleg`ahm rpj`
Lehnle` rpj` wmln` abbleg
1on hieh Xpncpzlh ahlb
Eir` rm` hiniz ic rm` zleg cizo`b Drm` kpehrlie
ic : nlqplbx
Ol} 1on% ic gnphix` xin“e # lon%
Aermzie` z`ag`er
Xraeb -xio`rlo`, # eir` hiniz acr`z 1 mz
Blnpr` wlrm gnahlan ah`rlh ahlb iz 04%
M:XI2
Bi rm` xao` wlrm 0% -ganahrix`. oanrix`. czphrix`.
xphzix`. nahrix`. gn|hig`e # xrazhm,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 14/36
H Tm`e|nm|bza{le` R`xr -Ixa{ie` Cizoarlie,
LLL Z@XPNRX AEB IJX@ZSARLIE
A Oinlxhm R`xr
Xinprlie Oinlxhm“x r`xr Aermzie` R`xr Tm`e|nm|bza{le`
R`xr
@}t`hr`b z`xpnr Tpztn` zleg Gz``e \`nniw/tan` izaeg`
blxrlnn`b war`z
-hierzin,
-/, Hn`az nlqplb E)A E)A
0% gnphix` -&, Tpztn` zleg -/, \`nniw/jnahf -&,\`nniw/jnahf
ganahrix` -&,Tpztn` zleg -/, Jziwe/jnahf -&,\`nniw/jnahf
oanrix` -&,Tpztn` zleg -/, jnahf -&,Izaeg`/jnahf
czphrix` -&,Tpztn` zleg -/, jnahf -&,jziwe
xphzix` -&,Tpztn` zleg -/, jnahf -&,Jziwe
nahrix` -&,Tpztn` zleg -/, \`nniw/jnahf -&,Izaeg`/jnahf
gn|hig`e -&,Tpztn` zleg -&,Gz``e/jnahf -&,Tan` |`nniw
xrazhm -&,Tpztn` zleg -&,Gz``e/jnahf -&,|`nniw
Le `ahm ic = r`xr rpj` tnah` 1on% ic 0 xin“e ic -(1/gnphix`. (:/
oanrix`. (8/czphrix`. (2/ganahrix`. (0/nahrix`. (3/xphzix`. (7/
gn|hig`e. (=/xrazhm,
Abb a tlehm ic tm`e|nm|bza{le` )r`xr rpj`
Ol} # xritt`z rm` r`xr rpj`x -pxleg hirrie,
Tnah` rm`o le jilnleg M:I ciz 84 ole
Hiin xniwn| D ziio r`ot`zarp`
@}aole` hz|xranx cizo`b peb`z a
olhzixhit`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 15/36
LS AEAN\XLX AEB HIEHNPXLIE
GPLB@ QP@XRLIE
1 Gls` rm` tzlehltn` lesins`b le `ahm r`xr ciz hazjim|bzar`x
A% Oinlxhm“x R`xr
Oinlxhm R`xr lx a x`exlrls` hm`olhan r`xr ciz ann hazjim|bzar`x aeb xio` hiotipebx
hieraleleg hazjim|bzar`x le a hiojle`b cizo. jax`b ie rm` b`m|bzarlie ic rm` hazjim|bzar` j|
xpncpzlh ahlb ri tzibph` ae anb`m|b` `lrm`z -cpzcpzan iz a b`zlsarls`, wmlhm rm`e hieb`ex`x wlrm rm`
tm`einlh xrzphrpz` z`xpnrleg le a tpztn`/hiniz`b hiotipeb Hazjim|bzar`x az` b`m|bzar`b j|
hieh`erzar`b xpncpzlh ahlb ri cizo m|bzi}|o`rm|ncpzcpzan wlnn z`ahr wlrm antma/eatmrmin -Oinlxhm
z`ag`er, ri |l`nb a tpztn` hieb`exarlie tzibphr
J Aermzie` R`xr
Le rm` aermzie` axxa|. hazjim|bzar`x az` b`m|bzar`b j| pxleg hieh M:XI2 ri cizo
cpzcpzan. wmlhm le rpze hieb`ex`x wlrm aermzie` -14/f`ri/<.14 blm|bziaermzah`e`, ri cizo a jnplxm/
gz``e hiotn`} Xpgazx z`ahr wlrm rm` aermzie` z`ag`er peb`z ahlblh hieblrliex ri |l`nb a jnp`/
gz``e hiniz Hieh`erzar`b M:XI2 lx a tiw`zcpn b`m|bzarleg ag`er lesins`b le b`m|bzarleg xpgazx
n`ableg ri cizoarlie ic rm` cpzcpzan. wmlhm hieb`ex`x rm` aermzie` ri gls` a hiniz`b a tzibphr
H Tm`e|nm|bza{le` R`xr -Ixa{ie` Cizoarlie,
Wm`e tm`e|nm|bza{le` z`ahr wlrm rm` z`bphleg xpgazx D jilnleg r`ot`zarpz`. ixa{ie` lx
cizo`b Tm`e|nm|bza{le` -EM:/EM/H3M0, z`ahrx wlrm hazjie|n hiotipebx le e`przan iz xnlgmrn|
ahlblh hieblrliex ri gls` tm`e|nm|bza{ie`. wmlhm lx mlgmn| xinpjn` Wm`e m|bza{ie`x z`ahr cpzrm`z
wlrm rm` tm`e|nm|bza{le` oin`hpn`. rm` hieb`exarlie tzibphrx cizo`b az` lexinpjn` ixa{ie`x.
wmlhm tz`hltlrar` ipr ax hz|xranx Z`bphleg xpgazx cizo hmazahr`zlxrlh ixa{ie` hz|xranx wm`e m`ar`b
wlrm ae `}h`xx tm`e|nm|bza{le` Rmlx tzit`zr| lx arrzljpr`b ri rm` tz`x`eh` ic anb`m|b` iz f`rie`
gzipt le rm`lz oin`hpn`x /IM gzipt loo`blar`n| abkah`er ri rm` f`ri gzipt lx i}lbl{`b ri a f`ri
gzipt aeb abbx ri tm`e|nm|bza{le`ri cizo rm` |`nniw ri tan` izaeg` ixa{ie`x
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 16/36
Aean|xlx
Le rm` Oinlxhm R`xr. czio rm` `}t`zlo`er ann xpgaz r`xr`b xinprliex |l`nb tixlrls` z`xpnr
xleh` rm`| az` ann hazjim|bzar`x Wmln` rm` hierzin rpj` wmlhm hieralex war`z gls` a e`garls` z`xpnrwmlhm lx a hn`az xinprlie% Xleh` lr“x eir a hazjim|bzar`%
Le rm` Aermzie` R`xr. ien| gn|hig`e # xrazhm gls` tixlrls` z`xpnrx jpr. xpttix`bn| ann xpgaz
xinprliex opxr gls` tixlrls` z`xpnrx le rm` Aermzie` R`xr xleh` rm`| az` ann hazjim|bzar`x jpr bp`
ri xio` cahrizx le rm` tzih`xx ic r`xrleg rm`o rmar“x wm| xio` blbe“r gas` tixlrls` z`xpnrx
Le rm` Ixa{ie` R`xr. ann r`xr`b xpgaz xinprliex gas` a |`nniw/tan` izaeg` hinizarlie Jpr
xio` xpgaz xinprliex. wm`e rm`| az` sl`w`b peb`z rm` olhzixhit` rm`| blbe“r gls` `}t`hr`b
z`xpnrx -hz|xranx cizoarlie, Rmlx lx oa|j` j`hapx` j| rm` rlo` gls`e. rm`| az` ajipr ri cizo
hz|xranx irm`z xpgaz r`xr`b xinprliex raf`x nieg`z rmae rm` rlo` gls`e rlo` ri cizo rm`lz hz|xranx
rmar“x wm| xio` blbe“r |l nb a tixlrls` hz|xranx cizoarlie -gnphix` # xphzix`,. wmln` irm`z |l`nb
tixlrls` z`xpnrx -ganahrix`. czphrix`. nahrix`. oanrix`. gn|hig`e # xrazhm,
Hiehnpxlie
Le hiehnpxlie. ciz rm` Ahrlslr| 3A Qpanlrarls` R`xrx ciz Blcc`z`er Hazjim|bzar`x; Pxleg
Oinlxhm. Aermzie` # Ixa{ie` qpanlrarls` r`xr w` j`hao` caolnlaz wlrm hiooie tzlehltn`x ic
hazjim|bzar`x le blcc`z`er qpanlrarls` r`xrx W` n`aze`b anxi rm`lz xlgelclhaer xlolnazlrl`x #
blcc`z`eh`x le rm`lz z`ahrliex le blcc`z`er qpanlrarls` r`xrx W` fe`w anxi rmar `}r`zean cahrizx le rm`
tzih`xx ic r`xrleg rm`o wipnb acc`hr rm`lz z`ahrliex # z`xpnrx
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 17/36
S% BIHPO@ERARLIE
Tm`e|nm|bza{le` R`xr (1; Gn|hig`e. ( : Nahrix`. (8 Ganahrix`. (2 Gnphix`. (0 Xphzix`. (3 Xrazhm.
(7 Oanrix`. (= Czphrix`
Aermzie` R`xrOinlxhm“x R`xr
Tm`e|nm|bza{le` R`xr
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 18/36
Ahrlslr| 3J
Qpanlrarls` R`xr Ciz Hazjim|bzar`x
L IJK@HRLS@X
@epo`zar` rm` qpanlrarls` r`xrx ciz sazlipx r|t`x ic hazjim|bzar`x
Blxhpxx rm` tzlehltn`x le `ahm r`xr
@}tnale rm` hmazahr`zlxrlh ic blcc`z`er r|t` hazjim|bzar`
LL XHM@OARLH BLAGZAO
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 19/36
LS% AEAN\XLX AEB HIEHNPXLIEX
GPLB@ QP@XRLIE;
1 Gls` rm` tzlehltn` ciz `ahm r`xr ciz hazjim|bzar`x
1 Oiiz`'x R`xr / Wm`e a xinprlie ic z`bphleg xpgaz lx m`ar`b wlrm ae anfanl -EaIM,. lr rpzex
|`nniw ri izaeg` aeb cleann| bazf jziwe. nlj`zarleg rm` ibiz ic hazao`n Rmlx lx bp` ri rm` nlj`zarlie
ic anb`m|b` wmlhm xpjx`qp`er tin|o`zl{`x ri cizo a z`xleipx xpjxraeh`. hazao`n
:C`mnleg“x r`xr/ a r`xr ciz rm` tz`x`eh` ic anb`m|b`x jpr eir f`rie`x -xrlnn z`bphleg xpgazx,
Lr lx ae anr`zearls` r`xr ic rm` J`e`blhr“x r`xr ax ri J`e`blhr“x r`xr j`leg oiz` x`exlrls`% Anb`m|b`x
az` b`r`hr`b j| rm` z`bphrlie ic rm` b``t jnp` xinprlie ic Hitt`z -LL, ri a z`b tz`hltlrar` ic
lexinpjn` Hitt`z i}lb` Rm` C`mnleg“x r`xr lx pxpann| px`b ri b`r`hr ciz rm` tz`x`eh` ic z`bphleg
xpgazx jpr lr lx eir xt`hlclh ciz anb`m|b`x
8 J`e`blhr'x R`xr / Aqp`ipx gnphix` lx ol}`b wlrm J`e`blhr'x z`ag`er. a xinprlie ic hitt`z
xpncar`. xiblpo m|bzi}lb`. aeb razrazlh ahlb Rm` ol}rpz` lx m`ar`b Hazjim|bzar`x wmlhm z`ahr wlrm
J`e`blhr'x z`ag`er ri z`bph` rm` jnp` hitt`z -LL, lie ri cizo a jzlhf z`b tz`hltlrar` ic hitt`z -L,
i}lb` az` hnaxxlcl`b ax z`bphleg xpgazx% Ax le C`mnleg“x r`xr. rm` z`bphleg xpgazx j`hapx` ic masleg
tir`erlann| cz`` anb`m|b` iz f`ri gzipt z`bph` hptzlh m|bzi}lb` le anfanle` xinprlie ri z`b hinipz`b
hptzipx i}lb` B`t`ebleg ie rm` xpgaz hieh`erzarlie |`nniw ri gz``e hinipz lx b`s`nit`b
2 E|naeb`z R`xr / Hpzz`ern|. rm` E|naeb`z“x r`xr lx px`b ri r`xr ciz rm` tz`x`eh` ic z`bphleg
xpgazx -xt`hlclhann| gnphix`, le pzle`% Jpr. jaxlhann|. rm` E|naeb`z“x r`xr lx xrlnn a r`xr ciz z`bphleg
xpgazx E|naeb`z“x r`xr b`t`ebx ptie rm` z`bphrlie ic jlxoprm xpjelrzar` le a w`afn| anfanle`
xinprlie ri o`rannlh jlxoprm% Rm` E|naeb`z“x z`ag`er lx a xinprlie hiotix`b ic a xinprlie ic
Zihm`nn` xanr -tiraxxlpo xiblpo razrzar`,. tiraxxlpo iz xiblpo m|bzi}lb`. aeb jlxoprm xpjelrzar`le war`z Rm` xiblpo m|bzi}lb` iz tiraxxlpo m|bzi}lb` le rm` E|naeb`z“x z`ag`er gls`x rm` anfanle`
`eslzieo`er le izb`z ciz rm` z`ahrlie ri ihhpz Rm` tiraxxlpo razrzar` x`zs`x ax rm` xins`er ciz rm`
jlxoprm xpjelrzar`
0 Jazci`b'x R`xr / Jazci`b'x z`ag`er. a ol}rpz` ic `rmaeilh -ah`rlh, ahlb aeb hitt`z-LL,
ah`rar`. lx hiojle`b wlrm rm` r`xr xinprlie aeb jiln`b A z`b hitt`z-LL, i}lb` tz`hltlrar` lx cizo`b
wlnn leblhar`x rm` tz`x`eh` ic z`bphleg xpgaz Rm` z`ahrlie wlnn j` e`garls` le rm` tz`x`eh` ic
blxahhmazlb` xpgazx j`hapx` rm`| az` w`af`z z`bphleg ag`erx Rmlx r`xr lx xt`hlclh ciz
oieixahhmazlb` Bp` ri rm` w`afn| ahlblh earpz` ic Jazci`b'x z`ag`er. lr lx z`bph`b ien| j|
oieixahhmazlb`x Lr lx jax`b ie rm` z`bphrlie ic hitt`z-LL, ah`rar` ri hitt`z-L, i}lb` -Hp:I,.
wmlhm cizox a jzlhf/z`b tz`hltlrar`
3 Tlhzlh Ahlb r`xr lx g`e`zann| a r`xr ciz rm` tz`x`eh` ic z`bphleg xpgazx Rm` z`ag`er px`b
wax rm` tlhzlh ahlb -|`nniw le hiniz, Le izb`z ciz a z`ahrlie j`rw``e rm` z`bphleg xpgaz aeb Tlhzlh
ahlb ri raf` tnah`. rm` xinprlie opxr j` anfanle`6 rmlx lx rm` tpztix` ic abbleg rm` 14% Xiblpo
Hazjiear` le rm` `}t`zlo`er Wm`e Tlhzlh ahlb z`ahrx wlrm rm` anb`m|b` iz f`ri gzipt ic rm`
z`bphleg xpgaz. lr rpzex leri a Tlhzaolh ahlb wmlhm gls`x ic rm` tixlrls` hiniz ic Oamigae| z`b
M`arleg wax bie` j`hapx` le mlgm`z r`ot`zarpz`x. z`ahrlie j`rw``e xpjxraeh`x raf`x tnah` caxr`z
7 X`nlwaeicc'x R`xr / Lr lx a hiniz z`ahrlie xt`hlclh ciz f`rix`x Wm`e hieh`erzar`b MHn lx
abb`b f`rix`x peb`zgi b`m|bzarlie ri |l`nb cpzcpzan b`zlsarls`x oiz` zatlbn| rmae anbix`x Rm`x`b`zlsarls`x cizo hiotn`}`x wlrm z`xizhlein ri |l`nb b``t z`b hiniz Rm` r`xr z`ag`er hapx`x rm`
b`m|bzarlie ic f`rim`}ix`x ri cizo 0/m|bzi}|o`rm|ncpzcpzan 0/m|bzi}|o`rm|ncpzcpzan z`ahrx wlrm
z`xizhlein tz`x`er le rm` r`xr z`ag`er ri tzibph` a b``t z`b tzibphr Lr lx a rlo`b hinipz z`ahrlie
xt`hlclh ciz f`rix`x
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 20/36
= Lible` R`xr / Rmlx r`xr lx px`b ciz rm` b`r`hrlie ic xrazhm le rm` xinprlie Rm` jnp` jnahf
hinipz lx bp` ri rm` cizoarlie ic xrazhm/lible` hiotn`}% Xrazhm hierale tin|o`z ic α/ao|nix` aeb
ao|nit`hrle wmlhm cizox a hiotn`} wlrm lible` ri gls` rm` jnp` ri tptn` jnahf hinipz
< Ophlh Ahlb r`xr lx a r`xr xt`hlclh ciz rm` oieixahhmazlb` ganahrix` Wm`e
oieixahhmazlb`x -ganahrix`, az` rz`ar`b wlrm xrzieg i}lbl{leg ag`erx xphm ax elrzlh ahlb iz MEI8-wax prlnl{`b le rm` `}t`zlo`er,. lr |l`nbx a xahhmazlh ahlb Rm` xahhmazlh ahlb ijrale`b acr`z rm`
i}lbarlie ic ganahrix` lx lexinpjn` aeb x`tazar`x ax hz|xranx Rmlx ahlb b`zlsarls` lx feiwe ax ophlh
ahlb Rmlx r`xr anniwx ciz rm` blcc`z`erlarlie ic ganahrix` ri irm`z oieixahhmazlb`x ax irm`z
oieixahhmazlb`x rmar peb`zgi rmlx r`xr |l`nb xinpjn` blhazji}|nlh ahlb wm`z`ax ganahrix` tzibph`x
lexinpjn` Ophlh ahlb Nahrix` lx a blo`z ic gnphix` aeb ganahrix` Rm` elrzlh ahlb wlnn m|bzin|{` rm`
blo`z aeb xi nahrix` wlnn anxi gls` a tixlrls` z`xpnr%
Aean|xlx
1% Oiiz`“x r`xr/Le ipz `}t`zlo`er rm` xinprlie rmar |l`nb a tixlrls` z`xpnr wax gnphix`. ganahrix`.
oanrix`. czphrix` aeb nahrix` wmln` xphzix`. gn|hig`e aeb xrazhm wax e`garls` aeb wax leblhar`b j|a hn`az xinprlie% Oiiz`“x r`xr z`ahrlie lx bp` ri rm` tz`x`eh` ic ae anb`m|b` wmlhm le rpze nlj`zar`b
ri cizo hazao`n aeb lr lx anxi xt`hlclh ciz z`bphleg xpgazx Gnphix`. ganahrix`. oanrix`. czphrix` aeb
nahrix` az` z`bphleg xpgazx wmln` xphzix`. gn|hig`e aeb xrazhm az` eir. rm`z`ciz` ipz `}t`zlo`eran
z`xpnrx az` hizz`hr Xphzix` blb eir gls` a tixlrls` z`xpnr le rm` r`xr j`hapx` lr lx eir a z`bphleg
xpgaz Rm` eie/z`bphleg tzit`zr| ic xphzix` lx bp` ri lr“x aeio`zlh hazjie wmlhm lx eir *cz``*
xleh` rmlx hazjie nlefx czphrix` aeb gnphix` rig`rm`z Rmlx aeio`zlh hazjie hae'r it`e pt rm` zleg
xrzphrpz` aeb z`ahr wlrm rm` z`ag`er -wmlhm lx Hieh`erzar`b EaIM, Xrazhm aeb gn|hig`e blb eir
z`ahr le rm` Oiiz`“x r`xr% Rmlx lx j`hapx` jirm ic rm`o az` lx a tin|xahhmazlb` Ann tin|xahhmazlb`x
az` eie/z`bphleg xpgazx rm`z`ciz`6 lrx aeio`zlh hazjie lx „cpnn‟ aeb haeeir z`ahr ri aeirm`z
oin`hpn`
:% C`mnleg“x R`xr/ Le ipz `}t`zlo`er rm` xinprlie rmar |l`nb a tixlrls` z`xpnr wax gnphix`. ganahrix`.
oanrix`. czphrix` aeb nahrix` wmln` xphzix`. gn|hig`e aeb xrazhm wax e`garls` aeb wax leblhar`b j|
a hn`az jp` xinprlie wlrm ei tz`hltlrar`% C`mnleg“x r`xr lx a r`xr xt`hlclh ciz z`bphleg xpgazx% Gnphix`.
ganahrix`. oanrix`. czphrix` aeb nahrix` az` z`bphleg xpgazx wmln xphzix`. gn|hig`e aeb xrazhm az`
eir Rmlx z`ahrlie mas` rm` xao` `}tnaearlie wlrm rm` oiiz`“x r`xr%
8% J`e`blhr“x R`xr/ Le ipz `}t`zlo`er rm` xinprlie rmar |l`nb a tixlrls` z`xpnr wax gnphix`. ganahrix`.
oanrix`. czphrix` aeb nahrix` wmln` xphzix`. gn|hig`e aeb xrazhm wax e`garls` aeb wax leblhar`b j|
a hn`az jp` xinprlie wlrm ei tz`hltlrar`% J`e`blhr nlf` C`mnleg“x lx a r`xr xt`hlclh ciz z`bphleg xpgazx
Rm` oale blcc`z`eh` j`rw``e J`e`blhr“x wlrm C`mnleg“x lx J`e`blhr“x r`xr lx oiz` x`exlrls`% Rm`
C`mnleg“x r`xr e``bx a xrzieg anfanle` hieblrlie le izb`z ciz a z`ahrlie ri raf` tnah`. penlf` le rm`
J`e`blhr“x r`xr wm`z` `s`e le a w`af anfanle` `eslzieo`er. a z`ahrlie hipnb xrlnn ihhpz%Gnphix`.ganahrix`. oanrix`. czphrix` aeb nahrix` az` z`bphleg xpgazx wmln` xphzix`. gn|hig`e aeb xrazhm az`
eir
2% E|naeb`z“x R`xr/ Le ipz `}t`zlo`er rm` xinprlie rmar |l`nb a tixlrls` z`xpnr wax gnphix`.
ganahrix`. oanrix`. czphrix` aeb nahrix` wmln` xphzix`. gn|hig`e aeb xrazhm wax e`garls` aeb wax
leblhar`b j| a hn`az xinprlie Ax o`erlie`b tz`slipxn|. rmlx r`xr lx xt`hlclh ciz z`bphleg xpgazx rmpx
cinniw rm` xao` `}tnaearlie wlrm Oiiz`“x r`xr ajipr rm` z`bphleg xpgaz%
0% Jazci`b“x R`xr/ / Le ipz `}t`zlo`er ann rm` xinprlie |l`nb`b a e`garls` z`xpnr Rm`iz`rlhann|
gnphix`. ganahrix`. oanrix`. czphrix` aeb nahrix` wlnn j` tixlrls` xleh` lr lx a z`bphleg xpgaz wmln`
xphzix`. gn|hig`e aeb xrazhm wlnn j` e`garls` xleh` lr lx a eie/z`bphleg xpgaz Rm` wzieg z`xpnr ic rm` r`xr lx oa|j` bp` ri rm` m`arleg tzih`xx bie` le rm` `}t`zlo`er Bpzleg rm` m`arleg ic rm`
xinprlie w` tpr lr le a war`z jarm aeb tnah` lr le a j`af`z Oa|j` rmar cahriz mleb`zx rm` z`ahrlie ic
m`ar aeb rmpx rm` z`b tz`hltlrar` wax eir x``e le rm` z`ahrlie
3 Tlhzlh Ahlb R`xr / Le ipz `}t`zlo`er ien| ganahrix`. oanrix`. aeb czphrix` gas` rm` oamigae| z`b
hiniz wm`e le cahr ann z`bphleg xpgazx lehnpbleg gnphix` aeb nahrix` xmipnb mas` gls`e a tixlrls`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 21/36
z`xpnr `}hnpbleg xphzix`. gn|hig`e. aeb xrazhm j`hapx` rm`| az` eie/z`bphleg xpgazx W` olgmr
mas` ae `zziz bileg rm` tzih`bpz` bpzleg rm` `}t`zlo`er rmpx calnleg ri mas` rm` ahrpan z`xpnrx
7 X`nlwaeicc'x R`xr /Le rmlx `}t`zlo`er w` gale`b a tixlrls` z`xpnr ciz czphrix` aeb xphzix` wmlhm
az` le cahr f`rix`x le earpz`% Rm`| rpze`b tlef le hiniz ax rm`| az` m`ar`b taxxleg X`nlwaeicc“x r`xr%
= Lible` R`xr /J`ciz` t`zcizoleg rm` r`xr. w` mab a tz`/hiehnpb` aexw`z rmar rm` xrazhm wlnn gls` a
tixlrls` jnp` jnahf hiniz wmln` rm` gn|hig`e wlnn eir Ptie t`zcizoleg rm` tzih`bpz`. w` mab
gale`b a xrazhm wmlhm lx jnp` jnahf le hiniz wmln` rm` gn|hig`e j`leg izaeg` Le ipz bihpo`erarlie
w` mab rwi hn`az xinprliex j`hapx` lr wax rm` `eb tzibphr ic rm` r`xr Rm`| mab nixr rm`lz hiniz
ptie abbleg bzitx ic xiblpo rmlixpncar`
< Ophlh ahlb R`xr ‘ T`zcizoleg rmlx `}t`zlo`er ganahrix`. czphrix`. aeb nahrix` gas` a tixlrls`
lexinpjn` ophlh ahlb Jaxleg ie rm` tzlehltn` ganahrix` aeb nahrix` opxr mas` a tixlrls` z`xpnr aeb
w` gale`b rmix` jpr masleg czphrix` j`leg tixlrls` j`hapx` lr lx war`z xinpjn` rmpx peajn` ri
hz|xrannl{`
Hiehnpxlie
Rm`z` az` oae| qpanlrarls` r`xrx ciz hazjim|bzar`x Rmlx r`xr lx b`xlge`b ri lb`erlc| rm` xt`hlclh
hazjim|bzar`x. wm`rm`z lr lx a oieixahhmazlb`. blxahhmazlb`. tin|xahhmazlb`. z`bphleg iz eie/
z`bphleg xpgaz% Oiiz`“x r`xr. C`mnleg“x r`xr. J`e`blhr“x r`xr. E|naeb`z“x r`xr aeb Tlhzlh ahlb r`xr az`
r`xrx ciz z`bphleg xpgazx Jazci`bx r`xr lx anxi ciz z`bphleg xpgaz jpr ien| xt`hlclh ciz
oieixahhmazalb`x X`nlwaeicc “x r`xr lx xt`hlclh ciz f`rix`x. Lible` r`xr ciz xrazhm aeb Ophlh ahlb
r`xr ciz ganahrix`
S BIHPO@ERARLIE
Oiiz`“x R`xr
Czio n`cr ri zlgmr; Oanrix`. Xrazhm.
Xphzix`. Gn|hig`e. Nahrix`. Czphrix`.
Gnphix`. Ganahrix`
C`mnleg“x R`xr
Czio n`cr ri zlgmr; Gnphix`. Ganahrix`.
Czphrix`. Nahrix`. Xphzix`. Oanrix`.
Xrazhm. Gn|hig`e
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 22/36
J`e`blhr“x R`xr
Czio n`cr ri zlgmr; Gnphix`. Ganahrix`.
Czphrix`. Oanrix`. Nahrix`. Xphzix`. Xrazhm.
Gn|hig`e
Jazci`b“x R`xr Czio n`cr ri zlgmr;
Gnphix`. Ganahrix`. Czphrix`. Oanrix`.
Nahrix`. Xphzix`. Xrazhm. Gn|hig`e
Tlhzlh Ahlb R`xr
Czio n`cr ri zlgmr;Ganahrix`. Oanrix` Czphrix`.
Nahrix`. Gnphix`. Xrazhm
E|naeb`z“x r`xr
Czio n`cr ri zlgmr;Czphrix`.
Oanrix`. Nahrix`. Gnphix`. Xrazhm.
Ganahrix`. Gn|hig`e
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 23/36
X`nlwaeicc“x R`xr
Czio n`cr ri zlgmr;Ganahrix`. Nahrix`. Gnphix`.
Czphrix`. Xphzix`. Oanrix`
Lible` R`xr
Czio n`cr ri zlgmr; Gn|hig`e. Xrazhm
Ophlh Ahlb R`xr
Czio n`cr ri zlgmr;Ganahrix`. Gnphix`. Nahrix`. Czphrix`.
Xphzix`
Ophlh Ahlb R`xr Olhzixhit`Czio n`cr ri zlgmr; Czphrix`. Nahrix`. Ganahrix`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 24/36
Ahrlslr| 7
Xanlsaz| Blg`xrlie
L IJK@HRLS@X
Ri b`r`zole` rm` eizoan tM ic xanlsa
Ri blxhpxx rm` `cc`hr ic r`ot`zarpz` ie rm` blg`xrlie ic xrazhm le xanlsa
Ri blxhpxx rm` blcc`z`er cahrizx lecnp`ehleg tr|anle ahrlslr|
LL XHM@OARLH BLAGZAO
A Z`ahrlie ic Xanlsa
Tz`taz` 8 r`xr rpj`x wlrm a c`w bzitx ic a z`xrleg xanlsa
J% Leizgaelh Oarr`z
R`xr rm` z`ahrlie wlrm;
Tm`eintmrman`le Nlropx Tat`z Hiegi Z`b
Tz`taz` 0 on ic xanlsa le a r`xr rpj`
Abb 0 bzitx ic Elrzlh Ahlb ri ahlblc|
M`ar ri jilnleg -ri z`ois` tzir`lex,
Blslb` leri 2 r`xr rpj`x6 px` rm` clnrzar`
Hmnizlb`x Tmixtmar`x Xpncar`x Hanhlpo
Abb 1 bzit ic
Xlns`z Elrzar`
xinprlie
Abb 1 bzit ic
Aooielpo
Oin|jbar`
Abb 1 bzit ic
Jazlpo Hmnizlb`
xinprlie
Abb 1 bzit ic
Tiraxxlpo
I}anar`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 25/36
H% Lecnp`eh` Ic R`ot`zarpz` Ie Tr|anle Ahrlslr|
B% Lecnp`eh` Ic Blnprlie Ic Tr|anle Ahrlslr|
Tz`taz` 1 on ic 1% xrazhm xinprlie le
2 r`xr rpj`x
R`xr Rpj` 1
Lh` War`z Ol}rpz`
R`xr Rpj` :
Ziio R`ot`zarpz`
R`xr Rpj` 8
8=◣ H
R`xr Rpj` 2
Jilnleg
R`ot`zarpz`
Abb 14 bzitx ic
Xanlsa aeb xmaf`
Abb 14 bzitx
Xanlsa aeb xmaf`
Abb 14 bzitx ic
Xanlsa aeb xmaf`
Abb 14 bzitx ic Xanlsa aeb xmaf`
w`nn
@s`z| 10 olepr`x ler`zsanx g`r a xaotn` czio
@ahm r`xr rpj` aeb r`xr ciz Lible` aeb J`e`blhrx
xinprliex
Anniw ri xraeb ciz ae mipz
Tz`taz` 3 r`xr rpj`x hieraleleg < on
Ic war`z `ahm
R`xr Rpj` 1 R`xr Rpj` : R`xr Rpj` 8 R`xr Rpj` 2 R`xr Rpj` 0 R`xr
Rpj` 3
Abb 1 on
Ic xanlsa
Abb 1 on ic
Ol}rpz`
Czio
R`xr rpj` 1
Abb 1on ic
Ol}rpz`
Czio
R`xr rpj` :
Abb 1on ic
Ol}rpz`
Czio
R`xr rpj` 8
Abb 1 on ic
Ol}rpz`
Czio
R`xr rpj` 2
Abb 1on
Ic ol}rpz`
Czio
R`xr rpj` 0
Xmaf` w`nn
R`xr wlrm Lible` r`xr aeb J`e`blhr'x z`ag`er
Tnah` ar war`z jarm ar r`ot`zarpz` ajipr
24◣h ciz :4 olepr`x
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 26/36
LLL Z@XPNRX AEB IJX@ZSARLIE
A%
Leblharizx Z`xpnrx aeb ijx`zsarliex
Tm`eintmrman`le Hinizn`xx
Jnp` Nlropx Tat`z Jnp` ri Z`b
Z`b Nlropx Tat`z Z`b ri Z`b
Hiegi Z`b Z`b
J
H%
B%
LS AEAN\XLX AEB HIEHNPXLIE
GPLB@ QP@XRLIE
1 Miw blb |ip feiw rmar blg`xrlie max raf`e tnah` le rm` oiprm9
Rm` xanlsaz| gnaebx le rm` oiprm x`hz`r` `e{|o`x wmlhm jz`af biwe ciib le rm` oiprm j`ciz` lr
gi`x ri rm` xrioahm. rm` e`}r xlr` ic blg`xrlie Ipz xanlsa hieralex ae `e{|o` rmar xrazrx jz`aflegbiwe nieg hazjim|bzar` oin`hpn`x ax xiie ax w` tpr ciib le ipz oiprm Xanlsaz| ao|nax` lx rm`
`e{|o` rmar j`glex xtnlrrleg atazr rm` jiebx j`rw``e gnphix` oin`hpn`x le a nieg hmale ic xrazhm
Nlf` le ipz `}t`zlo`er lc xanlsa lx tpr leri a r`xr rpj` wlrm xrazhm lr wlnn hies`zr rmlx leri xpgaz%
: @}tnale miw r`ot`zarpz` lecnp`eh` rm` tzih`xx ic blg`xrlie
Clnrzar` ri r`xr ciz; Z`xpnrx aeb Ijx`zsarliex
Hmnizlb`x Hnipb| wlrm wmlr` tz`hltlrar` -Tixlrls`,
Tmixtmar`x Tan` \ nniw wlrm a nlrrn` wmlr` tz`hltlrar` - Tixlrls`,
Xpncar`x \`nniw wlrm wmlr` tz`hltlrar` -Tixlrls`,
Hanhlpo Hnipb| wlrm wmlr` tz`hltlrar` -Tixlrls`,
Saz|leg R`ot`zarpz`x J`e`blhr'x R xr Lible` R xr
Lh` War`z Nlgmr Jnp` -E`garls`, \`nniw -E`garls`,
Ziio R ot`zarpz` Nlgmr Jnp` -E`garls`, \ nniw wlrm Bazf Jnahf Xtirx
-Tixlrls`,
8=◣h Nlgmr Jnp` -E`garls`, Jnahflxm Z`b -Tixlrls`,
Jilnleg R ot`zarpz` Nlgmr Jnp` -E`garls`, \ nniw - E`garls`,
R`xr Rpj` Ei% Lible` R xr J`e`blhr'x R`xr
R xr rpj` (1 \ nniw - E`garls`, Nlgmr Jnp` - E`garls`,
R xr rpj` (: Bazf \ nniw -E`garls`, Nlgmr Jnp` - E`garls`,
R xr rpj` (8 Gz``e ttr -E`garls`, Nlgmr`xr Jnp` -E`garls`,
R xr rpj` (2 \ nniw -E`garls`, B``t`xr Jnp` aoieg ann
-E`garls`,
R xr rpj` (0 Bazf \ nniw -E`garls`, Nlgmr Jnp` -E`garls`,
R xr Rpj` (3 Bazf`xr \ nniw-E`garls`, Nlgmr Jnp` -E`garls`
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 27/36
Blg`xrlie lx a hm`olhan z`ahrlie wm`z` `e{|o`x hn`as`x rm` hiotn`} oin`hpn`x leri xlotn` xpgaz iz
carr| ahlb oin`hpn`x Nlf` `s`z| hm`olhan z`ahrlie rm` blg`xrlie anxi e``bx haran|xr -`e{|o`,.
r`ot`zarpz`. aeb tM Lc rm` itrlopo r`ot`zarpz hmaeg`x rm` zar` ic z`ahrlie b`hz ax`x. xi ipz
jib| oaleralex hiexraer r`ot`zarpz` jpr xio`rlo`x r`ot`zarpz` hmaeg`x ie hmaeg` le tm|xlhan
hieblrliex iz j| oancpehrlie ic rm` jib| J| hmaeg` le r`ot`zarpz` `lrm`z b`hz`ax` iz lehz`ax`. rm`
zar` ic z`ahrlie b`hz`ax`x Lc xanlsa lx tpr leri a r`xr rpj` wlrm xrazhm lr wlnn hies`zr rmlx leri xpgaz Ar
niw r`ot`zarpz`x rmlx tzih`xx gi`x ie xniwn|. rm` s`nihlr| lehz`axleg ax rm` r`ot`zarpz` lehz`ax`x
perln lr z`ahm`x lrx oa}lopo ar ajipr 87´ H Ajis` rmlx r`ot`zarpz` rm` s`nihlr| agale b`hz`ax`x.
rm` `e{|o` j`leg b`xrzi|`b ar ajipr 74´ H
Aean|xlx
A% z`ahrlie ic xanlsa
Le rm` z`ahrlie ic xanlsa `}t`zlo`er. w` px`b blcc`z`er tM leblhariz
-tm`eintmrman`le. nlropx tat`z aeb hiegi z`b, ri j` ajn` ri `xrloar` rm` attzi}loar` tM
ic ipz xanlsa -z`xrleg, Ptie pxleg tm`eintmrman`le ax ipz leblhariz w` ijrale`b a hinizn`xx
xinprlie rmar leblhar`x rm` ahlblr| ic ipz xanlsa xinprlie Rm` Jnp` Nlropx Tat`z rpze`b ri
z`b aeb rm` Z`b Nlropx tat`z z`oale`b ax lr lx wmlhm agale xmiwx rm` ahlblr| ic ipz xanlsa
xinprlie Wm`e w` px`b rm` hiegi z`b leblhariz. a z`b xinprlie wax cizo`b wmlhm rm`e
leblhar`x rmar rm` tM ic ipz xanlsa xinprlie lx ajis` 0: wmlhm lx anoixr e`pzan W` rm`e
hiehnpb`b aeb `xrloar`b rmar rm` as`zag` tM ic ipz z`xrleg xanlsa zaeg`x czio ahlblh ri
e`przan Rm` `}t`zlo`er rmar w` hiebphr`b xpttizr`b rm` cahr rmar rm` itrloan tM ic ipz
z`xrleg xanlsa lx j`rw``e 32 ri 3< A z`ableg niw`z rmae 32 lx leblharls` ic lexpcclhl`er
anfanle` z`x`zs`x Acr`z `arleg. rm` xanlsa tM xmipnb zlx` ri 7= iz mlgm`z Pen`xx rmlx ihhpzx.
rm` jib| max anfanle` ole`zan b`clhl`ehl`x - oalen| Hanhlpo aeb Oage`xlpo , aeb wlnn eir
axxlolnar` ciib s`z| w`nn Ri b`slar` czio lb`an xanlsaz| tM ciz ae `}r`eb`b rlo` leslr`x
lnne`xx
J% Leizgaelh Oarr`z
Le rmlx `}t`zlo`er. w` r`xr`b ciz rm` tz`x`eh` ic hmnizlb`x. tmixtmar`x. xpncar`x
aeb hanhlpo le ipz xanlsa xinprlie Ri r`xr ciz tz`x`eh` ic hmnizlb` w` abb`b 1 bzit ic xlns`z
elrzar` xinprlie aeb wmlr` tz`hltlrar` wax cizo`b wmlhm leblhar`x a tixlrls` z`xpnr ciz
tz`x`eh` ic hmnizlb` Le rm` x`hieb r`xr rpj` w` r`xr`b ciz tmixtmar` j| abbleg 1 bzit ic
aooielpo oin|jbar`. rm` xinprlie rpze`b leri a tan` |`nniw wmlhm agale leblhar`x a
tixlrls` z`xpnr ciz tz`x`eh` ic tmixtmar` Rm` rmlzb r`xr rpj` wax px`b ri r`xr ciz xpncar
j| abbleg 1 bzit ic jazlpo hmnizlb`. a |`nniw xinprlie wlrm wmlr` tz`hltlrar` wax cizo`b
Rm` wmlr` tz`hltlrar` le rm`z` leblhar`x rmar xpncar` lx tz`x`er le ipz xanlsa xinprlie Le rm`
naxr r`xr rpj` w` r`xr`b ciz hanhlpo j| abbleg 1 bzit ic tiraxxlpo i}anar`. a hnipb|xinprlie wlrm wmlr` tz`hltlrar` wax cizo`b.wmlhm leblhar`x tixlrls` tz`x`eh` ic hanhlpo
W` rm`z`ciz` hiehnpb` rmar ipz xanlsa max leizgaelh hiotie`erx nlf` hmnizlb`x. tmixtmar`x.
xpncar`x aeb hanhlpo
H Lecnp`eh` Ic R`ot`zarpz` Ie Tr|anle Ahrlslr|
Le ipz `}t`zlo`er. w` gzabpann| gale`b e`garls` z`xpnrx ie jirm lible` aeb j`e`blhr“x r`xrx
Xleh` rm` r`xr oalen| cihpx`b ic rm` r`ot`zarpz` aeb feiwleg rmar xanlsa hieralex `e{|o`x. rm`
itrlopo n`s`n ic r`ot`zarpz` wax xpztaxx`b rmar“x wm| rm` r`xr blbe“r gas` rm` `}t`hr`b iprhio`%
Rm` xanlsa olgmr mas` eir hierale`b xrazhm acr`z ann xleh` rm` r`xr blbe“r mas` ae| tixlrls` z`xpnr%
B% Lecnp`eh` Ic Blnprlie Ic Tr|anle Ahrlslr|
Le ipz `}t`zlo`er. lr hae j` eir`b rmar rm` xinprlie |l`nb`b a e`garls` z`xpnr le jirm
j`e`blhr“x aeb lible` r`xr% Le rm` lible` r`xr rm` xinprlie rmar gls` rm` bazf`xr hiniz
z`ahrlie wax r`xr rpj` 3. L rmlef rmlx lx xio`miw hiecpxleg j`hapx` lr lx rm` oixr blnpr` aeb
ax `}t`hr`b lr xmipnb j` rm` nlgmr`xr Rmlx cahriz lx oa|j` j`hapx` rm` m`arleg ic rm`
xinprlie. rm` r`xr rpj`x wax eir s`en| `}tix`b ri m`ar
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 28/36
Hiehnpxlie
Rm` blg`xrls` cpehrliex ic xanlsa lehnpb` oilxr`eleg ciib aeb m`ntleg ri hz`ar` a ciib jinpx
Rmlx npjzlharls` cpehrlie ic xanlsa anniwx rm` ciib jinpx ri j` taxx`b `axln| czio rm` oiprm leri rm`
`xitmagpx Xanlsa hieralex rm` `e{|o` ao|nax` -anxi hann`b tr|anle,. aeb lx rmpx hatajn` ic jz`aflegbiwe xrazhm leri xlotn`z xpgazx rmar hae j` nar`z ajxizj`b iz cpzrm`z jzif`e biwe le rm` xoann
ler`xrle` Xanlsaz| gnaebx anxi x`hz`r` xanlsaz| nltax` -a oiz` tir`er cizo ic nltax`, ri j`gle car
blg`xrlie Xanlsaz| nltax` tna|x a nazg` zin` le car blg`xrlie le e`wjize lecaerx ax rm`lz taehz`arlh
nltax` xrlnn e``bx xio` rlo` ri b`s`nit Lr anxi max a tzir`hrls` cpehrlie. m`ntleg ri tz`s`er
jahr`zlan jplnb/pt ie rm` r``rm aeb waxmleg awa| abm`z`b ciib tazrlhn`x
S BIHPO@ERARLIE
Z`ahrlie ic xanlsa; czio n`cr ri zlgmr; tm`eintmrman`le. hiegi z`b. nlropx tat`z
Leizgaelh Oarr`z; czio n`cr ri zlgmr; xpncar`. hanhlpo. tmixtamr`x. hmnizlb`x
Lecnp`eh` Ic Blnprlie ic Tr|anle Ahrlslr|
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 29/36
Lecnp`eh` Ic R`ot`zarpz` le Tr|anle Ahrlslr|
+Czio n`cr ri zlgmr; xinprlie 1.:.8.2
Lible` R`xr
J`e`blhr'x R`xr
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 30/36
AHRLSLR\ 1:
PZLE@
L IJK@HRLS@X
Ri blxhpxx rm` tzlehltn` j`mleb `s`z| r`xr ciz pzle`
Ri blxhpxx rm` z`xpnrx aeb lrx lotnlharliex
Ri eao` rm` blcc`z`er blx`ax`x axxihlar`b wlrm `s`z| r`xr
LL XHM@OARLH BLAGZAO
Hz`arlele` -Elrzitzpxxlb` r`xr,
G Tarminiglhan hiexrlrp`erx;
Gnphix` J`e`blhr“x r`xr Anjpole -M`nn`z“x zleg r`xr,
Jln` tlgo`erx -Go`nle“x r`xr, Jln` ahlbx -T`rr`eficc`z“x r`xr,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 31/36
Ah`rie` r`xr -Elrzitzpxxlb` r`xr,
LLL Z@XPNRX AEB IJX@ZSARLIE
R@XR RM@IZ@RLHAN Z@XPNR @]T@ZLO@ERAN Z@XPNR
Z@XPNR IJX@ZSARLIE
G`e
hmazahr`zlxrlhx
Hiniz; tan` |`nniw ri b``t
aoj`z
Ibiz; xnlgmrn| eprr|
Hnazlr|; hn`az
Z`ahrlie; ahlblh
& Hiniz; ginb`e |`nniw
Xo`nn; aooielahan
Hnazlr|; hn`az
Z`ahrlie; ahlblh
Hz`arlele`
-elrzitzpxxlb`
r`xr,
Z`b ri |`nniw & Nlgmr |`nniw. hnipb|
Gnphix`
J`e`blhr“x r`xr
Gz``e & Gz``e w) |`nniw tz`hltlrar`
Anjpole
-M`nn`z“x zleg
r`xr,
Wmlr` zleg ar rm` kpehrlie ic
rm` rwi nlqplbx
& Ptt`z na|`z; hnipb| |`nniw
WMLR@ ZLEG LX TZ@X@ER
Niw`z na|`z; hn`az nlqplb
Jln` tlgo`erx
-Go`nle“x r`xr,
Gz``e iz slin`r / Ptt`z na|`z; |`nniw
Niw`z na|`z; tan` |`nniw
Jln` ahlbx
-T`rr`efic`z“x
r`xr,
Z`b / Ptt`z na|`z; jziwe
Niw`z na|`z; bazf jziwe.
anoixr jnahf
Ah`rie` r`xr
-elrzitzpxxlb`,
Izaeg` / Hn`az |`nniw
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 32/36
LS AEAN\XLX AEB HIEHNPXLIE
GPLB@ QP@XRLIE
1 Miw blb |ip lb`erlc| rm` tarminiglh hiexrlrp`erx ic pzle`9
L lb`erlcl`b rm` tarminiglh hiexrlrp`erx ic pzle` j| t`zcizoleg blcc`z`er r`xrx xphm ax
J`e`blhr“x r`xr ciz rm` tz`x`eh` ic gnphix`. M`nn`z“x zleg r`xr ciz anjpole. Go`nle“x r`xr ciz
jln` tlgo`erx. T`rr`efic`z“x r`xr ciz jln` ahlbx. aeb ah`rie` iz elrzitzpxxlb` r`xr
AEAN\XLX
A G`e`zan hmazahr`zlxrlhx
Pzle`“x g`e`zan hmazahr`zlxrlhx hae r`nn a nir ajipr a t`zxie“x m`anrm% Oae| rmlegx acc`hr
pzle` hinipz. lehnpbleg cnplb janaeh`. bl`r. o`blhle`x. aeb blx`ax`x Miw bazf iz nlgmr rm`
hinipz lx r`nnx |ip miw ophm war`z lx le lr Pzle` lx eizoann| hn`az Jahr`zla. jniib. xt`zo.
hz|xranx. iz ophpx hae oaf` pzle` niif hnipb| Pzle` bi`x eir xo`nn s`z| xrzieg. jpr max a
xnlgmrn| *eprr|* ibipz Xio` blx`ax`x hapx` a hmaeg` le rm` ibipz ic pzle` Ciz `}aotn`. ae
lec`hrlie wlrm @% hinl jahr`zla hae hapx` a jab ibipz. wmln` blaj`r`x iz xrazsarlie hae hapx`
a xw``r. czplr| ibipz Ax ciz z`ahrlie. pzle` lx ahlblh Xio`rlo`x. rm` tM ic pzle` lx acc`hr`b
j| h`zrale rz`aro`erx
C Hz`arlele`
Hz`arlele` lx eizoan le pzle` Lr lx a waxr`tzibphr rmar lx `}hz`r`b rmzipgm
pzlearlie Hz`arlele` z`cn`hrx rm` aoiper aeb ahrlslr| ic opxhn`x
M 1 Gnphix` J`e`blhr“x r`xr
Gnphix` lx tz`x`er le ipz xaotn` le s`z| xoann aoiperx Eizoann|. rm`z` lx s`z| nlrrn` iz
ei gnphix` le pzle` Wm`e jniib xpgaz lx s`z| mlgm. ax le blaj`r`x. rm` xpgaz xtlnnx is`z
leri rm` pzle` Gnphix` hae anxi j` cipeb le pzle` wm`e rm` flbe`|x az` baoag`b iz
blx`ax`b
: Anjpole -M`nn`z“x zleg r`xr,
Tzir`le lx eir cipeb le pzle` C`s`z. mazb `}`zhlx`. tz`geaeh|. aeb xio` blx`ax`x
-`xt`hlann| flbe`| blx`ax`x, oa| hapx` tzir`le ri j` le pzle`
8 Jln` tlgo`erx -Go`nle“x r`xr,
Jln` tlgo`erx le pzle` hae j` axxihlar`b wlrm kapeblh`. nls`z blx`ax`x. aeb m`tarlrlx Le
ipz xt`hlo`e. jln` tlgo`erx az` eir tz`x`er
2 Jln` ahlbx -T`rr`efic`z“x r`xr,
Jln` ahlbx le pzle` leblhar` nls`z blx`ax`x Jln` lx x|erm`xl{`b le rm` nls`z Lc jln` ahlbx az`
tz`x`er le pzle`. rm` nls`z oa| j` cpehrlieleg lotzit`zn| aeb xio` ic rm` jln` n`afx
leri rm` pzle`
0 Ah`rie` -elrzitzpxxlb` r`xr,
Ah`rie`. a f`rie`. lx eir tz`x`er le pzle` Wm`e ah`rie` lx tz`x`er. lr leblhar`x
f`riepzla. a o`blhan hieblrlie le wmlhm f`rie` jibl`x az` tz`x`er le pzle` Rmlx
matt`ex wm`e rm` jib| tzibph`x `}h`xx f`rie`x ax ae anr`zearls` xipzh` ic `e`zg|
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 33/36
j`hapx` ic rm` xmizrag` ic gnphix` Lr lx x``e bpzleg xrazsarlie iz le r|t` 1 blaj`r`x
o`nnlrpx
Hiehnpxlie
Rm`z`ciz`. L hiehnpb` rmar pzle` lx a s`z| `xx`erlan waxr` tzibphr ciz lr tzislb`x slran
lecizoarlie ajipr a t`zxie“x ler`zean cpehrlieleg% Anxi. a giib feiwn`bg` ajipr rm`
blcc`z`er r`xrx“ tzih`bpz`x aeb tzlehltn`x wipnb m`nt le b`r`zoleleg aeb peb`zxraebleg rm`
hieblrlie ic rm` leblslbpan
S BIHPO@ERARLIE
Pzle` -per`xr`b Hz`arlele` -elrzitzpxxlb` r`xr,
Gnphix` J`e`blhr“x r`xr Anjpole -M`nn`z“x zleg r`xr,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 34/36
Jln` tlgo`erx -Go`nle“x r`xr, Jln` ahlbx -T`rr`efic`z“x r`xr,
Ah`rie` r`xr -elrzitzpxxlb`,
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 35/36
AHRLSLR\ 18
JNIIB
L IJK@HRLS@X
Ri blxhpxx rm` tm|xlhan tzit`zrl`x ic jniib aeb lrx z`ahrliex
Ri blxhpxx rm` tzlehltn` j`mleb `s`z| r`xr ciz jniib
Ri lb`erlc| rm` blcc`z`er hiexrlrp`erx ic jniib
LL AEAN\XLX AEB HIEHNPXLIE
A Tm|xlhan tzit`zrl`x ic jniib
Jniib lx z`b le hinipz Rm` tM ic jniib lx j`rw``e 780/7206 m`eh`. lr lx xnlgmrn| jaxlh
Wm`e r`xr`b wlrm nlropx tat`z. lr rpze`b czio z`b ri jnp` aeb czio jnp` ri jnp`
J B`r`zolearlie ic jn``bleg rlo`Jn``bleg rlo` lx b`t`eb`er ptie rm` `cclhl`eh| ic rlxxp` cnplb le ahh`n`zarleg rm`
hiagpnarlie tzih`xx ie hatlnnaz| cpehrlie aeb rm` epoj`z ic tnar`n`rx tz`x`er aeb rm`lz
ajlnlr| ri cizo a tnar`n`r tnpg
H M`oin|xlx iz nafleg
M`oin|xlx. iz rm` jz`afleg biwe ic rm` ZJH“x o`ojzae`. hapxleg rm` z`n`ax` ic
ma`oignijle aeb irm`z ler`zean hiotie`erx leri rm` xpzzipebleg cnplb. oa| j` bp` ri
tarminiglhan hieblrliex -apriloope` ma`oin|rlh aea`ola iz rzaexcpxlie z`ahrlie, iz
lotzit`z xt`hlo`e hinn`hrlie. tzih`xxleg. iz rzaextizr Lr lx slxpann| b`r`hr`b j| xmiwleg a
tlef ri z`b rleg` le rm` x`zpo iz tnaxoa
B Hz`earlie
Lr lx a tm`eio`eie rmar matt`ex wm`e h`nnx az` `}tix`b ri a m|t`zrielh xinprlie Rmzipgm
ixoixlx. war`z cniwx czio az`ax ic niw xinpr` hieh`erzarliex ri az`ax ic mlgm xinpr`
hieh`erzarliex ri xrajlnl{` rm` rwi xinprliex Ax war`z n`as`x rm` h`nn. lr xmzls`nx iz
j`hio`x hz`ear`b
@ A J`e`blhr“x r`xr
Aqp`ipx xinprlie lx ol}`b wlrm J`e`blhr“x z`ag`er aeb m`ar`b% Hazjim|bzar`x wmlhm z`ahr
wlrm rm` z`ag`er ri z`bph` rm` jnp` hitt`z -LL, liex ri cizo a jzlhf z`b tz`hltlrar` ic hitt`z -L, i}lb` az` z`bphleg xpgazx
J Hmnizlb`
A r`xr ciz rm` tz`x`eh` ic hmnizlb`. ae `n`hrzin|r`. rmar wizfx wlrm irm`z `n`hrzin|r`x ri
f``t rm` tzit`z janaeh` ic jib| cnplbx aeb oalerale rm` jib|“x ahlb/jax` janaeh`
H Tmixtmar`
Lr lx t`zcizo`b ri x`` miw ophm tmixtmizpx lx le rm` jniib Rm` aoiper ic tmixtmar`
acc`hrx rm` n`s`n ic hanhlpo Ax jniib hanhlpo n`s`nx zlx`. tmixtmar` n`s`nx cann Jpr rmlx
z`narlie oa| j` blxzptr`b j| xio` blx`ax`x iz lec`hrliex
B Lzie
Lr tna|x a tzlehltan zin` le rm` x|erm`xlx ic ZJHx Lr lx e`h`xxaz| ciz rm` tzinlc`zarlie ic ZJHx
aeb ma`oignijle cpehrlie
7/23/2019 Biochem Customized Lab Manual FINALS
http://slidepdf.com/reader/full/biochem-customized-lab-manual-finals 36/36
C Cljzle r`xr
Rmlx r`xr o`axpz`x rm` aoiper ic cpehrliean cljzle tz`x`er le rm` tnaxoa Cljzle lx lesins`b
le jniib hiagpnarlie aeb tnar`n`r ahrlsarlie
G Xinpjlnlr| r`xr
Cljzle lx lexinpjn` le war`z j`hapx` lc lr lx eir xi. lr wlnn eir t`zcizo lrx cpehrlie ax ae
aerlhiagpnaer Z`o`oj`z. jniib lx tazr war`z
M Tzir`le hinipz r`xr
Cljzle wipnb j` tixlrls` le ann ic rm` r`xrx -Olnnie“x. Mitflex/Hin`. jlpz`r, Rm`x` r`xrx az`
r`xrx ciz tzir`lex. aeb cljzle lx earpzann| a tzir`le