Located within new St. Louis Community College Campus

Center for Plant and Life Sciences

BioBench Contract Research Organization

BioBench Contract Research Organization

Do you have a project you

need done?

Do you have time-consuming laboratory tasks that occupy your

personnel’s time?

BioBench Contract Research Organization

Do you lack access to necessary instrumentation for certain types of experiments?

BioBench Contract Research Organization

We can help….

BioBench Contract Research Organization

BioBench Contract Research Organization

We are the new

BioBench Contract Research Organization (CRO)

Located within the new St. Louis Community College campus

At the Center for Plant and Life Sciences

BioBench Contract Research Organization

Located within new St. Louis Community College CampusCenter for Plant and Life Sciences

BioBench Contract Research Organization

A few things we could help you with……

• Cloning – Fragment isolation– Ligation

• Isolation (large or small scale)– Genomic DNA– Plasmid DNA– RNA

• Agilent 2100– DNA, RNA and protein analysis

Nucleic Acid

• Sequencing– DNA isolation

– Sample preparation

– Sequence analysis

– Primer design

• Short Fragment Analysis– Genotyping

– Data interpretation

Capillary Electrophoresis

• Experienced with a large variety of cells– Adherent cell types– Suspension cell types

• Transient transfections– Lipid-based and peptide-based reagents– Electroporation and nucleofection

• Stable cell line generation

• Cryopreservation of cultures

Cell Culture

• Fluorescence Assisted Cell Sorting (FACS)

– For analysis and culture enrichment

• Labeling of cells in culture

• Fixing and Fluorescent Staining

• Staining of live cell cultures (direct immunofluorescence)

• Recording staining results by photography

Advanced Cell Culture Work

• Experimental design

• Screening of large number of siRNAs

• Detection of knockdown

• Data interpretation

RNA Interference

• SYBR Green and probe-based expertise

• Singleplex assay design (primer design)

• Multiplex assay design (primer/probe design)

• Assay optimization

• Extensive troubleshooting skills available– Can help with problematic existing assay (s)

• Data Analysis– Absolute quantitation– Comparative quantitation

Quantitative PCR

• Genotype Assay Development

• Screening of animal colonies

– Genomic DNA isolation from tail snips

– Transgenic or knockout animal identification

• Founder animal screening

Colony Work

• Various assays

– Luciferase

– Other activity-based assays

• Quantitation

• Immunoprecipitation (IP)

• SDS-PAGE

• Western Blotting

• ELISA

Protein

• If you have an idea, and you do not see it addressed here, please contact us….

• We are happy to work with you to

develop a project that will fit your needs

Don’t see what you need?

• Senior Research Scientist, CRO Coordinator

– Elizabeth (Betsy) Boedeker

• 314 513 4966

• eboedeker@stlcc.edu

• Director, Plant and Life Sciences Center

– Richard Norris, PhD

• 314 513 4951

• rnorris@stlcc.edu

Contact Information