bioanalytical & bioprocess - biogeniux · 2015. 9. 1. · detection of natural and recombinant...
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Bioanalytical & Bioprocess
Bioprocess & HCP ELISA
Biosimilar Conformational ELISA
Protein Refolding & Aggregation
PEGylated & PEG Immunogenicity ELISA
Hybridoma Screening Kit
Cell Cytotoxicity Assay
Endotoxin Recovery, Detection & Removal
Agarose Beads & Empty Columns
Interfering Protein Removal Kits
Lipid Removal & Clarification
PRODUCT NAME CAT. NO.
ENZ-51023
ENZ-51039
®ProteoStat Protein Aggregation Assay
®ProteoStat Protein Aggregation Standards
PRODUCT NAME
ENZ-51027
ENZ-51040
®ProteoStat Thermal Shift Stability Assay
®ProteoStat Protein Refolding & Aggregation Sensing Kit
Optimize & Monitor Biologics Integrity
The ProteoStat® Protein Aggregation Assay is a simple, sensitive, homogeneous fluorescent microplate assay. The novel molecular rotor dye used detects a broad range of different protein aggregates from visible to subvisible particles.
Ÿ Delivers superior results when compared to conventional protein detection dyes (Sypro Orange, Thioflavin T, ANS, Congo Red)
Ÿ High throughput method for monitoring protein stability when used with flow cytometry
Ÿ Convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
Ÿ Useful assay to troubleshoot protein damage, from protein development through manufacturing stage
The ProteoStat® Thermal Shift Stability Assay is a rapid, simple screening method based on a novel molecular rotor dye which detects protein aggregation.
Ÿ
Ÿ Screen for ligand binding to proteins of unknown function
Ÿ Screen protein variants to determine their relative stabilities or excipients to determine optimal protein storage conditions
Ÿ Identify protein-protein interaction inhibitors
Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
®ProteoStat Protein Aggregation Assay
®ProteoStat Thermal Shift Stability Assay
CAT. NO.
®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Protein A ELISA Kit
The Protein A ELISA kit is a sensitive and reproducible sandwich assay to quantify Protein A residuals in monoclonal antibody preparations. This extensively validated ELISA kit enables efficient detection of natural and recombinant Protein A constructs with up to 100% recovery.
Ÿ Detects 1ppm of Protein A residuals in human IgG
Ÿ Recognizes 4 different Protein A variants
Ÿ Useful for contamination analysis and measurement of Protein A variants in monoclonal antibody preparations
Ÿ Produces results in less than 3 hours with low cost per test
Natural
Protein A (Sa)
Millipore
RecombinantProtein A (Ec)
Repligen
Recombinant Cys-Protein A (Ec)
GE
Recombinant alkaline-resistant
Protein A (Ec)
GE MabSelet SuRe
ADI-900-057Protein A ELISA Kit
Bioprocess & Host Cell ProteinELISA Kits
The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis, Host Cell Protein quantitation and Western blots.
Ÿ Quantitatively measures host cell protein contamination in E.Coli and CHO derived biologics
Ÿ High throughput format with results in 2.5 hours
PRODUCT NAME
E. coli Host Cell Protein ELISA Kit*
CHO Host Cell Protein ELISA Kit*
His-Tag ELISA Kit
Bovine Albumin ELISA Kit
ENZ-KIT127
ENZ-KIT128
AKR-130
E11-113
CAT. NO.
Human Albumin ELISA Kit
Bovine IgA ELISA Kit
Bovine IgG ELISA Kit
Human IgA ELISA Kit
Human IgG ELISA Kit
Bovine IgM ELISA Kit
Human IgM ELISA Kit
Bovine Transferrin ELISA Kit
Human Transferrin ELISA Kit
Bovine Lactoferin ELISA Kit
GFP ELISA Kit
E88-129
E11-121
E11-118
E88-102
E88-104
E11-101
E88-100
E10-122
E88-128
E11-122
AKR-121
Antibodies & Protein Standards to detectHCPs using Western blot analysis
HCF1 (hamster), pAb
HCF1 (hamster), pAb (biotin conjugate)
HCF1 (E. coli), pAb (biotin conjugate)
IgG (human), pAb (biotin conjugate)
E. coli Host Cell Protein ELISA Standards
CHO Host Cell Protein ELISA Standards
E.coli HCP Chemiluminescent WB kit
HCF1 (E. coli), pAb
IgG (human), pAb
PRODUCT NAME CAT. NO.
ENZ-PRT121-0050
ENZ-PRT122-0050
KCAJ07
ENZ-ABS260-0100
ENZ-ABS261-0100
ENZ-ABS262-0100
ENZ-ABS263-0100
ENZ-ABS264-1000
ENZ-ABS265-1000
®Contamination Monitoring
*Manufactured under cGMP
Most Sensitive9.01 pg/ml
Assay recognition of different Protein A constructs, post boiling. Resulting concentrations were interpolated from kit standard curve. Percent recovery calculated by dividing observed concentration by expected concentration. A: Natural Protein A from S. aureus (Millipore), n=9; B: Recombinant Protein A from E. coli (Repligen), n=9; C: Recombinant Cys-Protein A from E. coli (GE), n=12; and D: Recombinant alkaline-resistant Protein A variant from E. coli (MabSelet SuRe from GE), n=12; graphical data represents statistical mean +/- 1 standard deviation. Ref.: Enzo Life Sciences
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Anti-peptide
Antibody
Antibody
Anti-human IgG
Biotinylated
Horse Radish
Peroxidase
H2N NH2
Steptavidin
Oxidized TMB
HN NH
TMB
Protein
Therapeutic
TMBioSims Conformational ELISA Kits
TMThe BioSims Conformational ELISA kits allow for 3-D conformational comparison, higher-order structure (HOS) characterization, between biosimilars and marketed mAb. This new technology provides a sensitive, systematic, and robust measurement of biosimilar conformation comparability at the molecular level.
Ÿ Unique measurement of biosimilar mAb HOS Comparability
Ÿ Detects small amounts of conformational impurity, ≥ 0.1%
Ÿ Correlates well with bioassays and glycosylation analysis
Reduce Hybridoma Screening & Cloning Time by 75%
TMThe DiSH Kit is a unique technology for the rapid production of hybridomas. Using a genetically-modified myeloma partner, the kit creates hybridomas capable of both secreting and surface-expressing antigen-specific antibodies, enabling screening and selection of positive clones by FACS. The components of the kit are available as standalone products as well.
Ÿ Enables faster, improved selection of specific, stable hybridomas
Ÿ Minimizes manipulation of cloned cell lines relative to approaches requiring Limiting Dilution Subcloning (LDS)
Ÿ Decreases time and labor costs
Hybridomas produce secreted antibody AND surface-bound antibody
Label cells with Fluorescent Antigen
Enrich cells with Antigen-coupled
Magnetic Bead Separation
Magnetic beads
Ag
Ag
OR FACS: Fluorescence-Activated Cell Sorting
PRODUCT NAME CAT. NO.
TMDiSH Kit
TMAbeoClone (HAT Semi-solid Medium)
ENZ-71001
ENZ-70004
TMAbeoClone (Base Semi-solid Medium) ENZ-70009
TMAbeoClone (CHO TCSC Semi-solid Medium) ENZ-70010
PRODUCT NAME CAT. NO.
Adalimumab Conformational ELISA
Alemtuzumab Conformational ELISA
Bevacizumab Conformational ELISA
Cetuximab Conformational ELISA
Conserved mAb Sequence Conformational ELISA
Infliximab Conformational ELISA
Palivizumab Conformational ELISA
Rituximab Conformational ELISA
Trastuzumab Conformational ELISA
ENZ-KIT122
ENZ-KIT119
ENZ-KIT118
ENZ-KIT120
ENZ-KIT123
ENZ-KIT124
ENZ-KIT126
ENZ-KIT125
ENZ-KIT121
The sensitive PEGylated Protein ELISA kit is ideal for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies.
Ÿ Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins
Ÿ Sensitive assay measures < 1ng/ml of PEGylated molecules
Ÿ Quantifies PEGylated target molecules in complex matrices to allow monitoring of drug levels or its accumulation in tissue
PRODUCT NAME CAT. NO.
PEGylated Protein ELISA Kit ADI-900-213
Improved Screening for Biotherapeutics®
Sensitive detection of linear and branched PEG molecules
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
PEGylated Protein ELISA Kit
The NIDS® PEG Immunogenicity ELISA provides a complete set of reagents to detect PEG-specific antibodies in human serum and other matrices.
• Double Antigen Bridging Assay in 96-well format
• Extensively tested with human serum samples
• 2.5 hour turnaround time
• Detects Abs to PEGs of various lengths and with different linking groups
• PEG Precoated plates
• Colorimetric, HRP based
• Limit of Detection: 125 ng/mL Ab
• No matrix effects observed
NIDS® PEG Immunogenicity ELISA
Depletion with 30 kDa PEG
Depletion with 40 kDa PEG
Recognizes PEG polymersof various sizes
NIDS® Streptavidin and Neutravidin Coated Plates
The Streptavidin or Neutravidin-coated ELISA plates are often used when coated proteins are in limited quantities or tend to be denatured during a direct coating process. NIDS® HyperBind High Sensitivity and High Binding streptavidin- and neutravidin-coated ELISA plates are available in clear, black, and white formats for colorimetric, fluorescence and chemiluminescence-based assays
PRODUCT NAME CAT. NO.
NIDS® Polyethylene Glycol (PEG) Immunogenicity ELISA Kit 11-1035-1KT
90-1030-10EA
1L-026-1KT
NIDS® DIY Immunogenicity ELISA Kit (10 Plates)
NIDS® PEG Immunogenicity Rapid Assay Kit (100 Tests)
Improved Screening for Biotherapeutics
• High Efficiency Coating
• Ready to use plates, pre-blocked with BSA
• Specific: High signal to noise ratios with low background
• Sensitive: Detection of < 1 ng/mL of protein is easily attainable
• Long shelf life: > 18 months at room temperature
• Low cost: Reduce reagent use by as much as 90%
0
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0.5
0.6
0.7
0.8
0.9
0.10 1.00 10.00 100.00 1000.00 10000.00
A 4
50
/63
0
Molar Ratio PEG: Ab
0
0.1
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0.5
0.6
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0.8
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A 4
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Molar Ratio PEG: Ab
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0.1 1 10 100 1000
A 4
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Molar Ratio PEG: Ab
Depletion with 20 kDa PEG
Improved Screening for Biotherapeutics ®
40 kDa PEG
H
YHRP
H-40 kDa PEG
ANPEG-1
Anti-H-HRP conjugate
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Sandwich ELISA for mouse IgG using NIDS® HyperBind streptavidin (SA) and neutravidin (NA) plates and the commercially available Pierce SA and NA plates. NIDS® HyperBind plates were coated at 0.5 μg/mL capture Ab and the Pierce plates at 5.0 μg/mL. NIDS® HyperBind plates generate greater signal and sensitivity while using only 10% of the antibody required by the commercial plates. *(Reference: Vallejo YR, Li J, Yin R. Enhancing assay performance using nanoscale detection. IVD Technology, Volume 16, May/June 2010)
PRODUCT NAME
High Sensitivity Plates
NIDS® HyperBind High Sensitivity Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Sensitivity Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Sensitivity Streptavidin Coated Black Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Sensitivity Streptavidin Coated White Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Sensitivity Streptavidin Coated Clear Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Sensitivity Neutravidin Coated Clear Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Sensitivity Streptavidin Coated Black Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Sensitivity Neutravidin Coated Black Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Sensitivity Streptavidin Coated White Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Sensitivity Neutravidin Coated White Stripwell Plates (96 Well) (300 plates)
High Binding Plates
NIDS® HyperBind High Binding Streptavidin Coated Clear Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Binding Neutravidin Coated Clear Solid Plates (96 Well) (5 Plates)
NIDS® HyperBind High Binding Streptavidin Coated Clear Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Binding Neutravidin Coated Clear Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High Binding Streptavidin Coated Black Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High-Binding Neutravidin Coated Black Stripwell Plate 96-Well (300 plates)
NIDS® HyperBind High Binding Streptavidin Coated White Stripwell Plates (96 Well) (300 plates)
NIDS® HyperBind High-Binding Neutravidin Coated White Stripwell Plate (96-Well) (300 plates)
NIDS® HyperBind High-Binding Neutravidin Coated Black Solid Plate (96-Well) (5 Plates)
11-1001-5PK
11-1007-5PK
12-1001-5PK
13-1001-5PK
21-1001-5PK
21-1007-5PK
22-1001-5PK
22-1007-5PK
23-1001-5PK
23-1007-5PK
11-3001-5PK
11-3007-5PK
21-3001-5PK
21-3007-5PK
22-3001-5PK
22-3007-5PK
23-3001-5PK
23-3007-5PK
12-3007-5PK
CAT. NO.
Improved Screening for Biotherapeutics®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Most sensitive ELISA plates for Serological Assays*streptavidin or neutravidin-coated
Comparison of TSH Assays on HyperBindand Commercial Streptavidin Plates
ANP Pierce HBC Pierce Nunc R&D systems
Binding Efficiency: HyperBind Streptavidin (SA)clear plates vs. commercial plates
Vendor 1 Vendor 2 Vendor 3HyperBind
Sandwich Assay Performance at OptimalCoating Concentrations
HyperBind NA plate,0.5μg/mL capture Ab Vendor 1 NA plate, 5.0μg/mL capture Ab
NIDS® DIY Biotinylation and Digoxigenin Labeling Kits
NIDS® DIY Biotinylation and Digoxigenin labeling Kits provide an easy way to label peptides and proteins rapidly and efficiently.
• Successfully used in PK and Immunogenicity Assays
• Short and long linkers are available
• Rapid QC test strip confirms successful labeling.
• Compared to other methods, which often require at least 100 µg of the conjugate, our QC procedure consumes only low nanograms of the labeled reagent.
NIDS® DIY Biotin-1500-NHS Antibody Labeling Kit
NIDS® DIY Biotin-1500-NHS Antibody Labeling Kit
NIDS® DIY Biotin-1500-NHS Protein Labeling Kit
NIDS® DIY Biotin-1500-NHS Protein Labeling Kit
NIDS® DIY Digoxigenin-NHS Antibody Labeling Kit
NIDS® DIY Digoxigenin-NHS Antibody Labeling Kit
NIDS® DIY Digoxigenin-NHS Protein Labeling Kit
NIDS® DIY Digoxigenin-NHS Protein Labeling Kit
PRODUCT NAME
90-1018-1KT
90-1018-10KT
90-1017-1KT
90-1017-10KT
90-1024-1KT
90-1024-10KT
90-1023-1KT
90-1023-10KT
CAT. NO.ANTIBODY/PROTEIN
AMOUNT
Sandwich Pharmacokinetics Assay Sandwich Immunogenicity Assay
Up to 2 mg
Up to 20 mg
Up to 2 mg
Up to 20 mg
Up to 2 mg
Up to 20 mg
Up to 2 mg
Up to 20 mg
Improved Screening for Biotherapeutics ®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Rapid QC Test Strips provided with the kit for confirmation of successful labeling of proteins
Improved Screening for Biotherapeutics®
A simple and easy-to-use kit capable of irreversible removal of interfering endogenous proteins such as drug target compounds, which can cause erroneous results in immunogenicity and PK assays
NIDS® NPX4000 Interfering Protein Removal Kits
PRODUCT NAME CAT. NO.
NIDS® NPX4000 Interfering Protein Extraction Kit (100 samples) (Generic DIY Kit) 90-1026
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
• Irreversibly removes >99% of endogenous interfering protein or drug target in patient samples
• Highly efficient & does not affect original ADA levels
• Simple extraction procedure easily adaptable to high throughput testing
• Significantly greater capacity than microparticles
• Capable of removing up to 400 ng/mL of interfering proteins
• Uses up to 100-fold less capture reagent than microparticles
• Suitable for all bioanalytical methods including PK and IM assays
Effect of Removal of Target Interference on an Immunogenicity Assay For a Biotherapeutic Monoclonal Antibody Using NPX4000 Extraction Particles
0 1 10 100
[Interfering Protein] ng/mL0 1 10 100
Sign
al
Before Extraction After Extraction
YY
Y Y
YY
YY
Y
NPX4000Particles
Serum Sample
Centrifugation
NPX4000 nanoparticles with scavenger/capture reagentY
Interfering protein
Use this kit to attach the specific blocking antibody or receptors to activated nanoparticles and pretreating the sample, followed by a centrifugation step, enables irreversible removal of interference without affecting the dection of ADAs
PRODUCT NAME PRODUCT NAME PRODUCT NAME PRODUCT NAMECAT. NO. CAT. NO.CAT. NO. CAT. NO.
NIDS® NPX4000 INTERFERING PROTEIN REMOVAL KITS
90-1147
90-1148
90-1149
90-1150
90-1151
90-1152
90-1153
90-1154
90-1155
90-1156
90-1157
90-1158
90-1159
90-1160
90-1161
90-1162
90-1163
90-1164
90-1165
90-1166
90-1167
90-1168
90-1169
90-1170
90-1171
90-1172
90-1173
90-1174
90-1175
90-1176
90-1177
90-1178
IL-12 p70
IL-13
IL-15
IL-16
IL-17
IL-1A
IL-1B
IL-1RA
IL-2
IL-3
IL-4
IL-5
IL-6
IL-6R
IL-7
IL-8
I-TAC
Leptin
LIGHT
Lymphotactin
MCP-1
MCP-2
MCP-3
MCP-4
M-CSF
MDC
MIF
MIG
MIP-1A
MIP-1B
MIP-1D
MIP-3A
90-1179
90-1180
90-1181
90-1182
90-1183
90-1184
90-1185
90-1186
90-1187
90-1188
90-1189
90-1190
90-1191
90-1192
90-1193
90-1194
90-1195
90-1196
90-1197
90-1198
90-1199
90-1200
90-1201
90-1202
90-1203
90-1204
90-1205
90-1206
90-1207
90-1208
90-1209
90-1210
MIP-3B
MSP-A
NAP-2
NT-3
NT-4
OPG
OSM
PARC
PCSK9
PDGF-BB
PIGF
RANTES
RF
SCF
SDF-1
sgp130
TARC
TECK
TGF-beta 1
TIMP-1
TIMP-2
TNF RI
TNF RII
TNF-a
TNF-b
TPO
TRAIL R3
TRAIL R4
TrkA
uPAR
VEGF
VEGF-D
Acrp30
AgRP
Amphiregulin
ANG
Angiopoietin-1
AXL
BDNF
bFGF
BLC
BMP-4
BMP-6
b-NGF
BTC
CCL28
CD28
CD3 epsilon
CD39/ENTPD1
CD4
CD5
Ck beta 8-1
CNTF
CTACK
Dtk
EGF
EGF-R
ENA-78
Eotaxin
Eotaxin-2
Eotaxin-3
EPO
ErbB2
Fas
90-1095
90-1096
90-1097
90-1098
90-1099
90-1100
90-1101
90-1102
90-1103
90-1104
90-1105
90-1106
90-1107
90-1108
90-1109
90-1110
90-1111
90-1112
90-1113
90-1114
90-1083
90-1084
90-1085
90-1086
90-1087
90-1088
90-1089
90-1090
90-1091
90-1092
90-1093
90-1094
FGF-4
FGF-6
FGF-7
FGF-9
Flt-3 ligand
Fractalkine
GCP-2
GCSF
GDNF
GITR
GITR-ligand
GM-CSF
GRO-A
HCC-4
HER2neu receptor
HGF
hGH
I-309
ICAM-1
ICAM-3
IFN-gamma
IFN ß-1a
IGF-1
IGF-1 SR
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IL-10
IL-11
IL-12 p40
90-1115
90-1116
90-1117
90-1118
90-1119
90-1120
90-1121
90-1122
90-1123
90-1124
90-1125
90-1126
90-1127
90-1128
90-1129
90-1130
90-1131
90-1132
90-1133
90-1134
90-1135
90-1136
90-1137
90-1138
90-1139
90-1140
90-1141
90-1142
90-1143
90-1144
90-1145
90-1146
®
List of 140 target proteins which can be extracted from test samples using specific antibodies. We can also prepare the specific extraction nanoparticle kit for your unique requirements.
Improved Screening for Biotherapeutics
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
TMaCella -TOX - Bioluminescence non-radioactive cytotoxicity Assay
A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.
TMaCella -TOX use the same general ADCC and CDC processes where target cell apoptosis is induced. This lysis leads to the release of endogenous GAPDH, an enzyme in glycolysis and gluconeogenesis pathways, from the cytoplasm. Through the addition of coupling enzymes, adenosine triphosphate (ATP) is generated, and when coupled to a luciferase/luciferin reaction, produces a measureable bioluminescent signal. This signal is proportional to the amount of GAPDH release and target cell lysis.
CLATOX100-3500
CLATOX100-3L500
CLATOX100-3P500
CLATOX100-41000
PRODUCT NAME CAT. NO.TEST
Non Radioactive Cytotoxicity Assay - aCella -TOXTM
Non Radioactive Cytotoxicity Assay - aCella -TOX (5 Lumi Plates)TM
Non Radioactive Cytotoxicity Assay - aCella -TOX (5 Lumi Plates + 5 Tissue Culture Plates)TM
Non Radioactive Cytotoxicity Assay - aCella -TOXTM
Specifications & Advantages
Versatile - Useful for measuring activity of T Cells, Primary Cells, NK, complement and other lytic agents. Assay can be run in serum supplemented media.
FAST - Results in 3-5 minutes. Chromium 51 or europium release for measurement are time consuming. The inherent sensitivity of luciferase detection is enhanced by the amplification effect of enzyme turnover, which produces thousands, millions or billions of high - energy molecules for each molecule of the enzyme.
Highly Sensitive - Can detect fewer than 500 cells/well in the presence of serum or as few as 10 cells/well in serum-free or heat killed media.
GAPDH- The fact that GAPDH is a natural component of cells, and does not need to be introduced into the cells in any manner, distinguishes this assay from all methods which require prelabelling of cells, transfection, transformation, or other methods of introducing proteins or other molecules into the target cells in order to generate a signal in a later step.
Advantages for measurement of cell mediated or complement mediated cytolysis - It is usually desirable to use smaller numbers of TCells than are needed for the 51Cr – release assay, since excessive numbers of effector cells can
TMincrease the background signal. This is now possible due to the high sensitivity of aCella -TOX.
Improved Screening for Biotherapeutics®
TMaCella -TOX assay principle
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
SMA
LL M
OLE
CU
LES
CEL
L D
EATH
OX
IDA
TIV
E ST
RES
S
Simplify your cell death analysis
Cyto-ID® Autophagy Detection Kit (ENZ-51031)
• No transfection required• Protocol validated with known
inhibitors and activators • Rapidly quantifies autophagy
in native heterogeneous cell populations
• Negligible staining of lysosomes reducing background
GFP-Certified Apoptosis/Necrosis Detection Kit (ENZ-51002)
• True multiplexing capabilities with GFP and other green fluorescent probes
• Readily distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells
• Optimized for both fluorescence microscopy and flow cytometry
TM
Sensitive assays for oxidative stress
High-throughput analysis of mitotoxicity
Cyto-ID® Hypoxia/Oxidative Stress Detection Kit (ENZ-51042)
• Sensitive hypoxia dye fluoresces red when converted by nitroreductates
• Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green)
• Non-toxic, cell permeable dye optimized to avoid artifactual fluorescence
• Suitable for microscopic or flow cytometry analysis of adherent or suspension cells
Mito-ID® Membrane Potential Cytotoxicity Assay Kit (ENZ-51019)
• 10x more sensitive than JC-1 with superior aqueous solubility
• Photostable dual-emission dye• Separate Mito-ID® Red/Green
assays available for detection of mitochondrial mass
• Suitable for high-throughput applications
Bioactives for Drug Development
Ultra-high Purity | Cost effective | Extensively Validated
PRODUCT NAME CAT. NO.
Compound Libraries
Autophagy Library BML-2837
Cardiotoxicity Library BML-2850
Hepatotoxicity Library BML-2851
Hematopoietic Toxicity Library BML-2852
ICCB Known Bioactives Library BML-2840
FDA Approved Drug Library V2 BML-2843
Bioactive Lipid Library BML-2800
Protease Inhibitor Library
Nephrotoxicity Library
BML-2833
ENZ-LIB100
PRODUCT NAME
Autophagy Modulators
3-Methyladenine
LY 294002
Rapamycin
Wortmannin
Osmotic Stress Inhibitors
JX-401
PD 169,316
SB202190
SB220025
Protease Inhibitors
Protease Inhibitor Cocktail
3,4-Dichloroisocoumarin
Actinonin
Chymostatin
Cystatin C (human)
CAT. NO.
BML-ST420
BML-ST415
BML-EI366
ALX-270-258
BML-EI294
ALX-270-325
BML-KI103
BML-PI110
ALX-260-128
ALX-260-005
ALX-850-292
BML-AP502
BML-A275
Solutions for Bioprocess Optimization ®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Cleanascite™ - Lipid adsorption and clarification reagent
Ÿ A high binding capacity for lipids with minimal cross-reactivity with proteins
Ÿ Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon) and it is environmentally friendly.
Ÿ Very low protein binding
Ÿ Does not bind to DNA, RNA, enzymes and proteins
Ÿ Extends the life of membrane and chromatographic columns.
Clarifies:-
• 5
Ascites
• 2
Serum/Plasma
• 7
Bile
• Cohn Paste
• 3
Cell Lysates
• 6
Tissue Culture
• Organ Homogenates
• 4
Saliva/Sputum
• Egg Yolk
• Transgenic Milk
Cleanascite™
Cleanascite™
Cleanascite™
Cleanascite™
100 ml
1 Liter
4 Liters
16 Liters
400 ml
4 Liters
16 Liters
64 Liters
X2555 -100
X2555- 1000
X2555- 4000
X2555- 16000
PRODUCT NAME SIZE CAT. NO.TOTAL SAMPLE VOLUME THAT CAN BE PROCESSED*
TMTreatement With Cleanascite
Insert: PAGE showingLeft: MarkersRight: IgY and other major protein fractionrecovered
Ideal reagent for delipidation treatments fordownstream processing of large-scale therapeuticproteins, enzymes and monoclonal antibodies.
Egg Yolk After (Left) and Before (Right)
Quality & Purity of Biotherapeutics®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Cleanascite™ selectively removes lipids, cell debris, lipoproteins, floating fats, impurities from Cohn paste, transgenic milk, egg yolk and biological samples for pretreatment of samples prior to purification.
The reagent is a solid-phase, non-ionic adsorbent supplied as a suspension in saline, ready for use. Simply add, centrifuge and/or filter. The clarified supernatant is ready for subsequent downstream processing or analysis.
*Based on Cleanascite™ to Sample typical volume ratio. Volume ratio may be adjusted according to lipid levels.
Agarose Beads for purification of Proteins
We offers a wide range of activated agarose beads for affinity chromatography and enzyme and antibody immobilization processes, as well as a variety of non-activated agarose beads for size exclusion chromatography.
PRODUCT NAME CAT. NO. PACK SIZE PRODUCT NAME CAT. N O. PACK SIZE PRODUCT NAME CAT. NO. PACK SIZE
AFFINITY CHROMATOGRAPHY PURIFICATION
HIS-TAG PURIFICATION
LOW PRESSURE: CHELATING AGAROSE BEADS: BULK RESINS
HIGH Density METAL FREE
HIGH Density NICKEL
HIGH Density ZINC
HIGH Density COBALT
LOW Density METAL FREE
LOW Density NICKEL
LOW Density ZINC
LOW Density COPPER
NICKEL CHELATE KIT 6BCL-KNi-2
NICKEL CHELATE KIT + 20 empty mini columns
6BCL-KNiSP-2
NICKEL & COBALT CHELATE KIT 6BCL-KNiCo-2
NICKEL & COBALT CHELATE KIT + 30 empty mini columns
6BCL-KNiCoSP-2
ZINC CHELATE KIT 6BCL-KZn-2
ZINC CHELATE KIT + 20 empty mini columns
6BCL-KZnSP-2
ZINC & COPPER CHELATE KIT 6BCL-KZnCu-2
ZINC & COPPER CHELATE KIT + 30 empty mini columns
6BCL-KZnCuSP-2
HIGH DENSITY CHELATE KIT
Quality & Purity of Biotherapeutics ®
HIGH DENSITY CHELATE KIT + 40 empty mini columns
LOW DENSITY CHELATE KIT
LOW DENSITY CHELATE KIT + 40 empty mini columns
LOW PRESSURE: NTA AGAROSE BEADS: BULK RESINS
NICKEL NTA Agarose Resin
HIGH PRESSURE: CHELATING RAPID RUN™ BEADS: BULK RESINS
NICKEL RAPID RUN™
5 ml
25 ml
100 ml
500 ml
COBALT RAPID RUN™
5 ml
25 ml
HIGH PRESSURE: RAPID RUN™ AGAROSE BEADS: PRE-PACKED COLUMNS
25 ml
100 ml
500 ml
100 ml
500 ml
ANTIBODY PURIFICATION
6BCL-KH-2
6BCL-KHSP-2
6BCL-KL-2
6BCL-KLSP-2
6NiRR-5
6NiRR-25
6NiRR-100
6NiRR-500
6CoRR-5
6CoRR-25
6BCL-QHNi-C8
6BCL-QHNi-C5
6BCL-QHZn-C8
6BCL-QHZn-C5
6BCL-QHCo-C8
6BCL-QHCo-C5
6BCL-QLNi-C8
6BCL-QLNi-C5
6BCL-QLZn-C8
6BCL-QLZn-C5
6BCL-QLCu-C8
6BCL-QLCu-C5
6BCL-NTANi-25
6BCL-NTANi-100
6BCL-NTANi-500
6CoRR-100
6CoRR-500
6RRFNi-Ctg1-2
6RRFNi-Ctg1-5
AF6Ni-Ctg5-1
AF6Ni-Ctg5-5
His-COLUMN HIGH Density NICKEL 8 x 1 ml
HisXL-COLUMN HIGH Density NICKEL 5 x 5 ml
His-COLUMN HIGH Density ZINC 8 x 1 ml
HisXL-COLUMN HIGH Density ZINC 5 x 5 ml
His-COLUMN HIGH Density COBALT 8 x 1 ml
HisXL-COLUMN HIGH Density COBALT 5 x 5 ml
His-COLUMN LOW Density NICKEL 8 x 1 ml
HisXL-COLUMN LOW Density NICKEL 5 x 5 ml
His-COLUMN LOW Density ZINC 8 x 1 ml
HisXL-COLUMN LOW Density ZINC 5 x 5 ml
His-COLUMN LOW Density COPPER 8 x 1 ml
HisXL-COLUMN LOW Density COPPER 5 x 5 ml
HIGH PRESSURE: CHELATING RAPID RUN™ BEADS: PRE-PACKED CARTRIDGE
NICKEL RAPID RUN™ Cartridges 1ml
NICKEL Affinity Cartridges 5ml
2 x 1ml
5 x 1ml
1 x 5ml
5 x 5ml
GST PURIFICATION
6BCL-QH-25 25 ml
6BCL-QH-100 100 ml
6BCL-QH-500 500 ml
6BCL-QHNi-25 25 ml
6BCL-QHNi-100 100 ml
6BCL-QHNi-500 500 ml
6BCL-QHZn-25 25 ml
6BCL-QHZn-100 100 ml
6BCL-QHZn-500 500 ml
6BCL-QHCo-25 25 ml
6BCL-QHCo-100 100 ml
6BCL-QHCo-500 500 ml
6BCL-QL-25 25 ml
6BCL-QL-100 100 ml
6BCL-QL-500 500 ml
6BCL-QLNi-25 25 ml
6BCL-QLNi-100 100 ml
6BCL-QLNi-500 500 ml
6BCL-QLZn-25 25 ml
6BCL-QLZn-100 100 ml
6BCL-QLZn-500 500 ml
6BCL-QLCu-25 25 ml
6BCL-QLCu-100 100 ml
6BCL-QLCu-500 500 ml
LOW PRESSURE: PROTEIN A AGAROSE BEADS: BULK RESINS
PROTEIN A Agarose Resin
PA09-R5 5 ml
PA09-R25 25 ml
PA09-R500
LOW PRESSURE: PROTEIN A AGAROSE BEADS: PRE-PACKED COLUMN
PROTEIN A Agarose Column PA09-C1-1
PROTEIN A Agarose XL Column PA09-C5-1
PROTEIN A Test Kit PA09-K-01
LOW PRESSURE: PROTEIN L AGAROSE BEADS: BULK RESINS
PROTEIN L Agarose Resin
PROTEIN L Test Kit
500 ml
1 x 1 ml
1 x 5 ml
1 X 100 µl
HIGH PRESSURE: PROTEIN A RAPID RUN BEADS: PRE-PACKED CARTRIDGE
TMPROTEIN A Rapid Run Cartridges
TMHIGH PRESSURE: AFFI-MAB BEADS: BULK RESINS
TMAFFI-MAB Fine Agarose Resin
TMAFFI-MAB Fine Test Kit
TMHIGH PRESSURE: PROTEIN G RAPID RUN BEADS: BULK RESINS
PROTEIN G Agarose Resin 4 RAPID RUN™
PROTEIN G Test Kit
4BCLPL-R2 2 ml
4BCLPL-R5 5 ml
4BCLPL-R10 10 ml
4BCLPL-K-01 1 X 100 µl
6RRFPA-Ctg1-2 2 x 1 ml
6RRFPA-Ctg1-5 5 x 1 ml
MABRRF-R10 10 ml
MABRRF-R25 25 ml
MABRRF-R100 100 ml
MABRRF-R200 200 ml
MABRRF-K-01 1 X 100 µl
4RRPG-1 1 ml
4RRPG-5 5 ml
4RRPG-25 25 ml
4RRPG-K-01 1 X 100 µl
GLUTATHIONE Agarose Beads4B-GLU-10 10 ml
4B-GLU-100 100 ml
LOW PRESSURE: GLUTATHIONE AGAROSE BEADS: BULK RESINS
Compatible
withTM TMAKTA & FPLC
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
PRODUCT NAME CAT. NO. PACK SIZE
Quality & Purity of Biotherapeutics®
PRODUCT NAME CAT. NO. PACK SIZE PRODUCT NAME CAT. NO. PACK SIZE
SIZE EXCLUSION CHROMATOGRAPHY
ACCESSORIES
EMPTY COLUMNS & EMPTY CARTRIDGES
PLASTIC MINI COLUMNS MC-100 100
EMPTY SPIN COLUMNS
PLASTIC SPIN COLUMNS SP-25 25
EMPTY COLUMNS
PLASTIC SMALL COLUMNS CS-20 20
PLASTIC COLUMNS C-50 50
PLASTIC XL COLUMNS CXL-50 50
EMPTY CARTRIDGES
EMPTY CARTRIDGES (1ml) EB-Ctg1-5 5
CARTRIDGES ACCESSORIES
STOP PLUG SEB-10 10
SYRINGE CONNECTOR SCEB-1 1
EMPTY FPLC COLUMNS
EMPTY FPLC 8 ml Columns FPLC8-3 3
EMPTY FPLC 30 ml Columns FPLC30-2 2
EMPTY FPLC 45 ml Columns FPLC45-2 2
EMPTY FPLC 80 ml Columns FPLC80-1 1
FPLC Luer lock-male FPLCLM-1 1
FPLC Luer lock-female FPLCLF-1 1
VERY LOW Density AMINOETHYL 4 RAPID RUN™
HIGH Density AMINOETHYL 4 RAPID RUN™
LOW Density AMINOETHYL 6 RAPID RUN™
HIGH Density AMINOETHYL 6 RAPID RUN™
VERY LOW Density AMINOETHYL 6 RAPID RUN™ FINE
ELECTROPHORESIS
AGAROSE POWDER
ABT Agarose LE
ABT Agarose HR
ABT Agarose GA
ABT Agarose LM
EMPTY MINI COLUMNS
4RR-AVL4-25 25 ml
4RR-AVL4-100 100 ml
4RR-AH1-25 25 ml
4RR-AH1-100 100 ml
6RR-AL0-25 25 ml
6RR-AL0-100 100 ml
6RR-AM3-25 25 ml
6RR-AM3-100 100 ml
6RRF-AVL4-25 25 ml
6RRF-AVL4-100 100 ml
A-1270-100 100 g
A-1270-500 500 g
A-1280-100 100 g
A-1280-500 500 g
A-1290-100 100 g
A-1290-500 500 g
A-1300-25 25 g
A-1300-100 100 g
HIGH PRESSURE: AMINOETHYL RAPID RUN™ BEADS: BULK RESINS
AGAROSES
EMPTY FPLC COLUMNS SUPPLIES
AFFINITY COUPLING
AMINO GROUPS
LOW Density GLYOXAL 4BCL
HIGH Density GLYOXAL 4BCL
LOW Density GLYOXAL 6BCL
HIGH Density GLYOXAL 6BCL
VERY HIGH Density GLYOXAL 6BCL
GLYOXAL KIT GLYOXK-2
LOW Density GLYOXAL 4 RAPID RUN™
HIGH Density GLYOXAL4 RAPID RUN™
LOW Density GLYOXAL4 RAPID RUN™ FINE
LOW Density GLYOXAL6 RAPID RUN™
HIGH Density GLYOXAL6 RAPID RUN™
LOW Density GLYOXAL6 RAPID RUN™ FINE
VERY LOW Density AMINOETHYL 4BCL
HIGH Density AMINOETHYL 4BCL
LOW Density AMINOETHYL 6BCL
HIGH Density AMINOETHYL 6BCL
AMINOETHYL LOW KIT AMINOL-2
AMINOETHYL COMPLETE KIT AMINOC-2
6% BCL Agarose Bead FINE (20-50µm)
4% RAPID RUN™ Agarose Bead STANDARD (50-150µm)
6% RAPID RUN™ Agarose Bead STANDARD (50-150µm)
A-1061F-250 250 ml
A-1061F-500 500 ml
A-1061F-1000 1 l
4RRS-500 500 ml
4RRS-1000 1 l
4RRS-10000 10 l
6RRS-500 500 ml
6RRS-1000 1 l
6RRS-10000 10 l
4BCL-GL0-25
4BCL-GL0-100
4BCL-GH1-25
4BCL-GH1-100
6BCL-GL0-25
6BCL-GL0-100
6BCL-GM3-25
6BCL-GM3-100
6BCL-GH1-25
6BCL-GH1-100
25 ml
100 ml
25 ml
100 ml
25 ml
100 ml
25 ml
100 ml
25 ml
100 ml
4RR-GL0-25 25 ml
4RR-GL0-100 100 ml
4RR-GH1-25 25 ml
4RR-GH1-100 100 ml
4RRF-GL0-25 25 ml
4RRF-GL0-100 100 ml
4RR-GL0-25 25 ml
4RR-GL0-100 100 ml
4RR-GM3-25 25 ml
4RR-GM3-100 100 ml
4RRF-GL0-25 25 ml
4RRF-GL0-100 100 ml
4BCL-AVL4-25 25 ml
4BCL-AVL4-100 100 ml
4BCL-AH1-25 25 ml
4BCL-AH1-100 100 ml
6BCL-AL0-25 25 ml
6BCL-AL0-100 100 ml
6BCL-AM3-25 25 ml
6BCL-AM3-100 100 ml
HIGH PRESSURE: RAPID RUN™ AGAROSE BEADS
LOW PRESSURE: GLYOXAL AGAROSE BEADS: BULK RESINS
HIGH PRESSURE: GLYOXAL RAPID RUN™ BEADS: BULK RESINS
ACIDIC GROUPS
LOW PRESSURE: AMINOETHYL AGAROSE BEADS : BULK RESINS
2% BCL Agarose Bead STANDARD (50-150µm)
4% BCL Agarose Bead STANDARD (50-150µm)
6% BCL Agarose Bead STANDARD (50-150µm)
8% BCL Agarose Bead STANDARD (50-150µm)
10% BCL Agarose Bead STANDARD (50-150µm)
4% BCL Agarose Bead MACRO(1) (150-350µm)
6% BCL Agarose Bead MACRO(1)
(150-350µm)
10% BCL Agarose Bead MACRO(1) (150-350µm)
4% BCL Agarose Bead FINE (20-50µm)
A-1021S-500 500 ml
A-1021S-1000 1 l
A-1021S-10000 10 l
A-1041S-500 500 ml
A-1041S-1000 1 l
A-1041S-10000 10 l
A-1061S-500 500 ml
A-1061S-1000 1 l
A-1061S-10000 10 l
A-1081S-500 500 ml
A-1081S-1000 1 l
A-1081S-10000 10 l
A-1101S-500 500 ml
A-1101S-1000 1 l
A-1101S-10000 10 l
A-1041M-500 500 ml
A-1041M-1000 1 l
A-1061M-500 500 ml
A-1061M-1000 1 l
A-1101M-500 500 ml
A-1101M-1000 1 l
A-1041F-250 250 ml
A-1041F-500 500 ml
A-1041F-1000 1 l
2% B Agarose Bead STANDARD (50-150µm)
4% B Agarose Bead STANDARD (50-150µm)
6% B Agarose Bead STANDARD (50-150µm)
8% B Agarose Bead STANDARD (50-150µm)
10% B Agarose Bead STANDARD (50-150µm)
(1) 4% B Agarose Bead MACRO(150-350µm)
(1) 6% B Agarose Bead MACRO(150-350µm)
(1)10% B Agarose Bead MACRO (150-350µm)
4% B Agarose Bead FINE (20-50µm)
6% B Agarose Bead FINE (20-50µm)
A-1020S-500
A-1020S-1000
A-1020S-10000
500 ml
1 l
10 l
A-1040S-500 500 ml
A-1040S-1000 1 l
A-1040S-10000 10 l
A-1060S-500 500 ml
A-1060S-1000 1 l
A-1060S-10000 10 l
A-1080S-500 500 ml
A-1080S-1000 1 l
A-1080S-10000 10 l
A-1100S-500 500 ml
A-1100S-1000 1 l
A-1100S-10000 10 l
A-1040M-500 500 ml
A-1040M-1000 1 l
A-1060M-500 500 ml
A-1060M-1000 1 l
A-1100M-500 500 ml
A-1100M-1000 1 l
A-1040F-250 250 ml
A-1040F-500 500 ml
A-1040F-1000 1 l
A-1060F-250 250 ml
A-1060F-500 500 ml
A-1060F-1000 1 l
LOW PRESSURE: PLAIN AGAROSE BEADS
LOW PRESSURE: CROSSLINKED AGAROSE BEADS
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Quality & Purity of Biotherapeutics ®
Ready to use HPLC lectin Columns
AffiSep® HPLC lectin columns are designed for the analysis, separation and purification of glycoproteins and other glycoconjugates containing lectin-specific carbohydrate residues. The columns are ready-to-use for operation with common HPLC or FPLC equipments and facilitate lectin affinity seperations of glycoproteins in a simple way for fast and reproducible results.
AffiSep® HPLC lectin columns specifications
CAT. NO. PRODUCT DESCRIPTION LECTIN
COVALENTLYIMMOBILIZED LECTIN(mg/ml ADSORBENT)
AMOUNT OF BOUNDGLYCOPROTEIN
(ug/0.6 ml ADSORBENT)SIZE
011031011032011033011034
011041011042011043011044
011051011052011053011054
011061011062011063011064
011071011072011073 011074
011082
011091 011092011093
011121011122011123011124
011131011132011133011134
AffiSep® WGA column
AffiSep® ConA column
AffiSep® LCH column
AffiSep® PNA column
AffiSep® AIL column
AffiSep® VVL column
AffiSep® AAL column
AffiSep® SNA column
AffiSep® MAL column
Triticumvulgaris
Canavaliaensiformis
Lensculinaris
Arachishypogaea
Artocarpusintegrifolia
Hairy vetch
Aleuriaaurantia
Sambucusnigra
Maackiaamurensis
~6
~15
~3
~3
~3
~3
~3
~3
~3
≥ 100
≥ 150
≥ 135
≥ 125
≥ 215
-
-
≥ 50
≥ 125
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL 4.0 mL
1.6 mL
0.8 mL1.6 mL2.5 mL
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
• Pore diameter: 1000 Å• Mean diameter particles: 75 µm
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
CAT. NO. PRODUCT DESCRIPTION LECTIN
COVALENTLYIMMOBILIZED LECTIN(mg/ml ADSORBENT)
AMOUNT OF BOUNDGLYCOPROTEIN
(ug/0.6 ml ADSORBENT)SIZE
Storage:
• Lectin columns should be stored equilibrated in adsorption buffer containing sodium azide at 4 °C.
Handling of buffers and samples:
• All buffer solutions should be filtered through 0,2 µm.
• All samples should be solved in adsorption buffer and filtered through 0,2 µm.
Protocol for column handling:
• Equilibrate column for 30 min with adsorption buffer (flow: 0,8 mL/min)
• Inject prepared sample solution and wash the column with adsorption buffer
• Elute the sample for at least 4 min with the recommended lectin-specific elution buffer at 0,8 mL/min.
• Equilibrate with adsorption buffer for 60 minutes for regeneration.
Operational conditions for Lectin columns:
• Max. column backpressure: 100 PSI (7 bar)
• Recommended flow rate: 0,6 - 0,8 mL/min
• Max. flow rate: 1,2 mL/min
• Detection wavelength: 280 nm for glycoproteins, 300 nm for p-Nitrophenyl-carbohydrates
• Range of pH: AffiSep®HPLC lectin columns can be operated between pH 4.0 and pH 8.0
011141011142011143011144
011151011152011153011154
011161011162011163011164
AffiSep® UEA column
AffiSep® GNA column
AffiSep® ECL column
Ulexeuropaeus
Galanthusnivalis
Erythrinacristagalli
~3
~3
~5
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
0.8 mL1.6 mL2.5 mL4.0 mL
-
≥ 200
≥ 100
Quality & Purity of Biotherapeutics®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Ÿ
biopharmaceutical samples containing surfactants
Ÿ Demasking independent of masking time and endotoxin concentration
Ÿ All needed reagents for developing a formulation-specific protocol included in the kit
Ÿ Detailed technical guidelines provided
Full quantitative endotoxin recovery in
Specifications & Advantages
Endo-RS® (Demasking of Endotoxin)
Endotoxin Recovery Kits (Surfactant)
Endo-RS® Endotoxin Recovery Kit is a novel sample preparation kit enabling complete endotoxin recovery in biopharmaceutical drug formulations containing non-ionic surfactants such as Polysorbate 20, Polysorbate 80 or Octylphenol etho xylates.
Non-ionic surfactants are well known to cause Low Endotoxin Recovery (LER). Intensive studies of the phenomenon have shown that the physicochemical proper ties of surfactants render endotoxin molecules inaccessible (masked) for detection in such samples. The Endo-RS® Endotoxin Recovery Kit now provides the essential reagents and a detailed guideline for evaluating and establishing an optimized endo-toxin demasking protocol for each individual formulation containing surfactants.
Applying the Endo-RS® Endotoxin Recovery Kit prior to endotoxin detection with the EndoLISA® Endotoxin Detection Assay will ensure FDA compliant and accurate results in spike validation studies and routine quality control testing.
Endo-RS® Protocol:
1. Screening:- Selection of the agents suitable for the specific formulation
2. Optimization:- Adjustment of protocol
3. Detection:- Quantification of demasked (accessible) endotoxin with the EndoLISA® assay
Endo-RS® Endotoxin Recovery Kit 609065
PRODUCT NAME CAT. NO.
Toolbox for demasking endotoxin in biopharmaceuticalformulations containing surface active agents
Quality & Purity of Biotherapeutics ®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Endotoxin Detection Assays
EndoZyme® is a homogeneous fluorescence end-point assay for the quantitative determination of endotoxins (lipopolysaccharides). The assay is based on an improved recombinant Factor C (rFC), the endotoxin-specific principal receptor in the LAL enzyme cascade. With a dynamic range of 0-50 EU/ml and a sensitivity of 0.005 EU/ml, EndoZyme® offers state-of-the-art test performance.
EndoZyme® specifically detects endotoxin thereby overcoming the problem with false-positive results due to β-Glucans. EndoZyme® is particularly suited as endotoxin test for biopharmaceuticals, medical devices, water and environmental swabs.
EndoZyme® correlation with LAL-Assay
Specifications & Advantages
Standard curve of the EndoZyme® Assay; concent- rations of the LPS standard are plotted against the corresponding relative fluorescence signal. The log net fluorescence is proportional to the log endotoxin concentration and is linear in the 0.005-50 EU/ml range
EndoZyme® endotoxin detection standard curve
rFC Recommended as LAL alternative in EU & USPharmacopoeia (EP,5.1.10) & (USP33,NF28,6.30)
Better Alternativeto LAL Test
Correlation plot of EndoZyme® versus LAL assay where multiple LPS preparations and dilutions from different bacteria such as E. coli, Salmonella and Pseudomonas, including LPS of mutant strains, were compared. The endotoxin was determined quantitatively according to the manufacturer’s instructions and the results (EU/ml) were plotted against each other. This comparison shows that EndoZyme® correlates well with the commercially available LAL test, with a
2correlation coefficient (R ) of 0.9476.
EndoZyme® (Enhanced Recombinant Factor C)
Quality & Purity of Biotherapentics®
EndoZyme®- 192 tests 609050
PRODUCT NAME CAT. NO.
Ÿ
Ÿ Broad measurement range 0.005-50 EU/ml
Ÿ LPS-specific recombinant technology
Ÿ No false-positive ß-Glucan reaction
Ÿ Excellent reliability and lot-to-lot consistency
Ÿ All needed reagents included in the kit
Ÿ No animal material, therefore saving the diminishing horseshoe crab population
Highly sensitive endotoxin detection assay
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
EndoLISA® (ELISA Based)
EndoLISA® is a new method for determination of endotoxin (LPS) levels where high-affinity binding of endotoxin by LPS-specific phage protein is followed by endpoint fluorescence measurement.
The microtiter plate -based assay format is one of the major advantage of EndoLISA®, as a result of which potentially interfering substances such as organic solvents, chaotropic substances and detergents are removed by a washing step. This enables the EndoLISA® user to precisely determine the endotoxin level without matrix effects, without the need for dilution and with a very broad measurement range.
Standard curve of the EndoLISA® test; concentrations of the LPS standard are plotted against the relative fluorescence signal. EU, endotoxin unit.
EndoLISA® correlation with LAL-Assay
EndoLISA® endotoxin detection standard curve
Correlation plot of EndoLISA® versus LAL assay where multiple LPS preparations and dilutions from different bacteria such as E. coli, Salmonella and Pseudomonas, including LPS of mutant strains, were compared. The endotoxin was determined quantitatively according to the manufacturer’s instructions and the results (EU/ml) were plotted against each other. This comparison shows that EndoLISA® correlates well with the commercially available LAL -assay, with a correlation coefficient of 0.9078.
rFC Recommended as LAL alternative in EU & USPharmacopoeia (EP,5.1.10) & (USP33,NF28,6.30)
Better Alternativeto LAL Test Specifications & Advantages
Quality & Purity of Biotherapeutics ®
EndoLISA®- 192 tests 609033
PRODUCT NAME CAT. NO.
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Ÿ Overcomes limitations of existing methods, such as the need for extension dilution
Ÿ Reduced matrix effects due to integrated washing step
Ÿ Robust assay with excellent reproducibility
Ÿ Broad measurement range (0.05-500 EU/ml)
Ÿ Broad pH range (pH4 - pH9)
Ÿ Less interference at high salt conditions e.g. NaCl, GdnHCl
Ÿ All reagents necessary to run the assay included in the kit
Ÿ Saves the diminishing horseshoe crab population
EndoTrap® (Affinity Chromotography Resins)
Endotoxin Removal Kits
EndoTrap® affinity chromatography for highly specific & efficient endotoxin (LPS) removal. The EndoTrap® ligand is a highly LPS-specific, completely non-toxic protein derived from bacteriophages. EndoTrap® removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rates.
EndoTrap® can be used in column (gravity flow) or batch mode and is suitable for up-scaling on fully automated liquid-chromatographic systems.
EndoTrap ® red EndoTrap ® blue EndoTrap ® HD
LPS 2,000,000 EU/ml resin
3 times
4-9
Suitable for high salt
conditions conditions
(> 150 mM NaCl)
2,000,000 EU/ml
resin
3 times
6-9
Calcium chelators
> 5,000,000 EU/ml resin
More than 10 times
4-10
Suitable for high salt
(> 150 mM NaCl)
Binding capacity:
PH sample buffer:
Sample Applications
Suitable Buffers:
Reusability:
Proteins,
Peptides,
Antibodies
Proteins,
Peptides,
Antibodies,
DNA/RNA,
Antigens,
Plant extracts
Proteins,
Peptides,
Antibodies,
DNA/RNA,
Antigens,
Plant extracts,
Automated systems,
Biomanufacturing processes
*Measured values for BSA, MAK 33 (monoclonal antibody) and POD (peroxidase) with EndoTrap HD ®
Specifications & Advantages
Ÿ Best-in-class endotoxin binding capacity of 5,000,000 EU/ml
Ÿ Removes more than 99.99%* of LPS
Ÿ Excellent sample recovery rate > 95%*
Ÿ Non-toxic phage-derived ligand, no cytotoxic effects
Ÿ Re-use more than 10 times
Ÿ Available in large quantities for up-scaling in biopharmaceutical processes
Quality & Purity of Biotherapeutics®
Removal Rate>99.99%
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
CAT. NO.
321053, 321063321064, 321075321066
311053, 311063311064, 311075311066
800053, 800063
800033
800034, 800035800036, 800037
PRODUCT NAME DESCRIPTION
EndoTrap® red (1/1, 5/1, 10, 50, Bulk)*
Available as pre-filled columns and settles resin.
EndoTrap® HD (1/1, 5/1, 10, 50, 250, Bulk)*
Available as pre-filled columns and settles resin.
EndoTrap® Leakage ELISA (for blue)
EndoTrap® HD & EndoTrap®
EndoTrap® blue (1/1, 5/1, 10, 50, Bulk)*
Available as pre-filled columns and settles resin.
For use with e.g. PBS bufferedsamples
For use with e.g. HEPES, TRISMOPS, MES, PIPES bufferedsamples
For biotech/pharmaevaluation andproduction processes
12x8 well strips, 1 vial POD-Antibody1 vial Standard20 ml ABTS Substrate
*(1/1 = 1 x 1 ml column, 5/1 = 5 x 1 ml column, 10 = 10 ml resin etc.)
EndoGrade®
Endotoxin-free Reagents and Accessories
Microbiological contamination is a well recognized problem when research reagents are used in certain applications. In particular increased endotoxin levels often lead to artefacts and misinterpretations in cell culture or animal model experiments. With our EndoGrade® product range, we meet our customers demand for endotoxin-free reagents and accessories.
PRODUCT NAME PRODUCT
800050
607030
607100
607500
321000
321001
321002
EndoGrade Glass Test Tubes 112 units per pack
EndoGrade Water 30 ml 20 bottles per pack
EndoGrade Water 100 ml 16 bottles per pack
EndoGrade Water 500 ml 6 bottles per pack
EndoGrade Ovalbumin 10 mg
EndoGrade Ovalbumin 100 mg
EndoGrade Ovalbumin 1000 mg
Quality & Purity of Biotherapeutics ®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Automation- Lab Essentials®
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
3Trinity V - Industrial Microbiology System
3Trinity VAn optional colony counting module is available. This complete industrial microbiology system is often used in pharmaceutical QA/QC microbiology laboratories.
utilizes digital imaging to automate zone reading and calculations for antibiotic potency assays.
• Follows USP, EP, BP and JP agar diffusion assay methods
• 21 CFR Part 11 compliant
Ozone Sterilizer- unique PORTABLE laboratory sterilization system
Ÿ Sterilizes surfaces & air spaces in multiple areas & locations
Ÿ Reduction or elimination of Bacteria, Fungus, Viruses, Protozoa, Yeast & Algae
Ÿ No harmful liquids, chemicals, UV rays, or heat.
Ÿ Keeps equipment, tools, and work areas clean without chemical residue or damage
Ÿ Auto De-ozonation at 240 cfm at the end of a sterilization cycle
PRODUCT NAME CAT. NO.
Ozilla™ Ozone Sterilizer, 220V E400220
Antibiotic Potency Zone Reading
Colony Counting
• Automatically counts bacterial, yeast colonies and virus plaque assays on agar plates
• Saves 99% of reading time
3Trinity V
TMOzilla Ozone Sterlizer
Automate and standardize zone reading and calculations for antibiotic potency
ENCLOSURE TYPE
Tissue Culture Hood (3 feet wide)
Tissue Culture Hood (4 feet wide)
Tissue Culture Hood (5-6 feet wide)
Room
MIN. STERILIZATION TIME
4.5 hours
6 hours
8 hours
12 hours
Incubator 2 hours
Applications
*The Ozilla sterilizer is designed for use in sealed cabinets or rooms, and is not to be used in open spaces where people and animals are present
Ÿ
Ÿ IQ/OQ/PQ validation documents
Ÿ 21 inch touch screen
Instant digital reading & calculations
Vial Filling System
10uL to 5mL dispense range
• Reliable, precise, an accurate dispensing
• Up to 8 different solutions simultaneously
• Compact and stand alone design
• Easy XY programming
• Flexible configuration
•
Protégé Vial Filling System
Phone : +91-11-4875-4875 Email : [email protected] Fax : +91-11-2561-2008
Automation- Liquid Handling ®
HTS WASHING HTS COATING & WASHING
• Expandable to >1,000 plates/hour
• 350 plates/hour@100uL dispense with <2% CV
• 96 & 384 well plates & vial filling capable
• 8 independent channelswashing & filling in one pass
• Stand-alone wash & aspiration unit
• Operating programs are self-contained – no external computer required
• Dual function– Micro-plate
HTS DISPENSING
TriFLEX Microplate Coating& Washing System
300-96WW Dual MicroplateWashing System
300 DMC Microplate DispensingSystem
Stand-alone wash and aspiration unit
• Up to 6 wash cycles (fill and aspirate) per pass
• Coaxial wash/aspirate manifold provides fast dispensing & thorough aspiration
•
Biogenuix Medsystems Pvt. Ltd.
412-B, Jyoti Shikhar Building, District Center,Janakpuri,
New Delhi-110058, INDIA
Phone : +91-11-4875-4875 • Fax : +91-11-2561-2008
E-mail : [email protected] • Web : www.biogenuix.com
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