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BINF 634 Fall 2013 - Lec 11 Visualization 1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based in Part on Previous Lecture of J. Grefenstette Outline

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Page 1: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

BINF 634 Fall 2013 - Lec 11 Visualization 1

Plan for Tonight

A synopsis of our progress through the course

Lecture 11 Visualization in PERL

Lab 2

Based in Part on Previous Lecture of J. Grefenstette

Outline

Page 2: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

BINF 634 Fall 2013 - Lec 11 Visualization 2

Where We are and Where We are Going

Program 1, Program 2, turned in and graded Midterm turned in and graded Program 3 turned in, Program 4 assigned tonight

Program 4 due in two week (11/25/13) Quiz 1, Quiz 2, Quiz 3, Quiz 4, lab1 turned in and

graded Lab 2 assigned tonight (11/11/13)

Lab 2 Due next week 11/18/13 Take home final provided 11/25/13

Take home final due to me 12/16/13

Miles to go …

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BINF 634 Fall 2013 - Lec 11 Visualization 3

Data Visualization Numbers are well and good, but often we get more insight by

visualizing data

As always, it's a good idea to break the process down into individual steps:data analysis script => output file => plotting script

=> chart file

This can all be wrapped for Web access:CGI script to get user input =>data analysis script => output file => plotting script

=> chart file=> CGI script displays chart on web page

Viz strategyBased in Part on Previous Lecture of J. Grefenstette

Page 4: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

BINF 634 Fall 2013 - Lec 11 Visualization 4

GD::Graph

Created by Martien Verbruggen

GD::Graph is a Perl module to create charts using the GD module. The following classes for graphs with axes are defined:

GD::Graph::lines -- Create a line chart

GD::Graph::bars and GD::Graph::hbars-- Create a bar chart with vertical or horizontal bars.

GD::Graph::points-- Create an chart, displaying the data as points.

GD::Graph::linespoints-- Combination of lines and points.

GD::Graph::area-- Create a graph, representing the data as areas under a line.

GD::Graph::pie-- Create a pie chart.

GDBased in Part on Previous Lecture of J. Grefenstette

Page 5: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

#!/usr/bin/perluse strict;use GD::Graph::bars;# File: bargraph.pl (based on http://linuxgazette.net/issue83/padala.html)

# All data arrays should have the same number of entriesmy @x = ("Jan", "Feb", "Mar", "Apr", "May", "Jun", "Jul", "Aug",

"Sep", "Oct", "Nov", "Dec");my @y = (23, 5, 2, 20, 11, 33, 7, 31, 77, 18, 65, 52);

# create a 2-dimensional array, where row 1 is x and row 2 is ymy @data = (\@x, \@y);

#create a new bar graph object and give it size (in pixels)my $graph = GD::Graph::bars->new(500, 300);

# set graph features such as labels on the axes, title$graph->set( x_label=>'Month', y_label=>'Number of Hits',

title=>'Number of Hits in Each Month in 2002')or warn $graph->error;

# plot the data to create an image objectmy $image = $graph->plot(\@data) or die $graph->error;

# print the image as a PNG file formatopen IMG, ">hist.png" or die "Can't open hist.png\n";print IMG $image->png;exit; 5BINF 634 Fall 2013 - Lec 11 Visualization

GDBased in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 6

Output

% open hist.png

GDBased in Part on Previous Lecture of J. Grefenstette

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#!/usr/bin/perluse strict;use CGI qw(:standard);use GD::Graph::bars;# File: bargraph.cgi

# All data arrays should have the same number of entriesmy @x = ("Jan", "Feb", "Mar", "Apr", "May", "Jun", "Jul", "Aug",

"Sep", "Oct", "Nov", "Dec");my @y = (23, 5, 2, 20, 11, 33, 7, 31, 77, 18, 65, 52);

# create a 2-dimensional array, where row 1 is x and row 2 is ymy @data = (\@x, \@y);

#create a new bar graph object and give it size (in pixels)my $graph = GD::Graph::bars->new(500, 300);

# set graph features such as labels on the axes, title$graph->set( x_label=>'Month', y_label=>'Number of Hits',

title=>'Number of Hits in Each Month in 2002')or warn $graph->error;

# plot the data to create an image objectmy $image = $graph->plot(\@data) or die $graph->error;

# print the image as a PNG file formatprint "Content-type: image/png\n\n";print $image->png;

7BINF 634 Fall 2013 - Lec 11 Visualization

Let’s create a web version !

GD

Based in Part on Previous Lecture of J. Grefenstette

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8BINF 634 Fall 2013 - Lec 11 Visualization

GDBased in Part on Previous Lecture of J. Grefenstette

Page 9: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

#!/usr/bin/perluse strict;use GD::Graph::bars;# File: bargraph2.pl

# read a file to get the data:my @x = (); my @y = (); open FH, "hist.dat" or die;while (<FH>) { my ($xval, $yval) = split; push @x, $xval; push @y, $yval }close FH;

# create a 2-dimensional array, where row 1 is x and row 2 is ymy @data = (\@x, \@y);

#create a new bar graph object and give it size (in pixels)my $graph = GD::Graph::bars->new(500, 300);

# set graph features such as labels on the axes, title$graph->set( x_label=>'Month', y_label=>'Number of Hits',

title=>'Number of Hits in Each Month in 2002')or warn $graph->error;

# plot the data to create an image objectmy $image = $graph->plot(\@data) or die $graph->error;

# print the image as a PNG file formatopen IMG, ">hist.png" or die "Can't open hist.png\n";print IMG $image->png; 9

BINF 634 Fall 2013 - Lec 11 Visualization

Plotting points from a file.

GD

Based in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 10

% cat hist.dat

Jan 23

Feb 5

Mar 2

Apr 20

May 11

Jun 33

Jul 7

Aug 31

Sep 77

Oct 18

Nov 65

Dec 52

GDBased in Part on Previous Lecture of J. Grefenstette

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#!/usr/bin/perluse strict;use GD::Graph::linespoints;# File: plot.plmy @x = (); my @y = ();

# read a file to get the data:open FH, "hist.dat" or die;while (<FH>) { my ($xval, $yval) = split; push @x, $xval; push @y, $yval }close FH;

# create a 2-dimensional array, where row 1 is x and row 2 is ymy @data = (\@x, \@y);

#create a new bar graph object and give it size (in pixels)my $graph = GD::Graph::linespoints->new(500, 300);

# set graph features such as labels on the axes, title$graph->set( x_label=>'Month', y_label=>'Number of Hits',

title=>'Number of Hits in Each Month in 2002')or warn $graph->error;

# plot the data to create an image objectmy $image = $graph->plot(\@data) or die $graph->error;

# print the image as a PNG file formatopen IMG, ">out.png" or die "Can't open out.png\n";print IMG $image->png; 11BINF 634 Fall 2013 - Lec 11 Visualization

Creating a plot with lines and points.

GD

Based in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 12

GDBased in Part on Previous Lecture of J. Grefenstette

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Getting the Chart on the Web This can all be wrapped for Web access:

CGI script to get user input (e.g., wrapper) =>data analysis script => output file => plotting script (e.g., plot.pl) => chart file (e.g.,

out.png) CGI script (wrapper) displays chart on web page

To display a file on the web, it has to be in a readable directory in the ~/public_html directory tree

1. Create a directory that can be written to by the web server process:

% mkdir ~/public_html/cgi-images% chmod 777 ~/public_html/cgi-images

13BINF 634 Fall 2013 - Lec 11 Visualization

GD

Based in Part on Previous Lecture of J. Grefenstette

Page 14: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

Getting the Chart on the Web2. In the wrapper, create the graphics file in the working directory3. Copy it to the images directory4. Display it using print img{src=>...}

use CGI qw(:standard); ...

# change to working directorymkdir $dir or die "Can't create directory $dir\n";chdir $dir or die "Can't change to directory $dir\n";

# create the data file for plottingsystem "cp /userhomes/faculty/jsolka/binf634/visualization/hist.dat $dir";

# run the plotting programsystem "/userhomes/faculty/jsolka/binf634/visualization/linegraph.pl";

# copy the output image to the web images directorysystem "cp out.png

/userhomes/faculty/jsolka/public_html/cgi-images/out.png";

# display image file on the web pageprint img {src=>"/jsolka/cgi-images/out.png"};

14BINF 634 Fall 2013 - Lec 11 Visualization

GD

Based in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 15

Limitations to GD::Graph

GD::Graph is good for simple graphs but has some severe limits for scientific plotting

X-axis data assumed evenly spaced 2-dimensional Limited control over labels, arrows, etc.

GDBased in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 16

gnuplot

gnuplot is an open source Unix application for creating high quality plots from data

http://www.gnuplot.info/ Runs interactively and in batch mode Interactive mode:

% gnuplot> plot sin(x)

gnuplotBased in Part on Previous Lecture of J. Grefenstette

Page 17: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

gnuplot Batch mode

put commands into a file, for example, histogram.plt run gnuplot with the command file as argument

% gnuplot histogram.plt

% cat histogram.plt# file histogram.pltset xlabel "Length"set ylabel "Sequences"set title "Sequence Length Distribution"plot "plot.dat" with boxesexit

17BINF 634 Fall 2013 - Lec 11 Visualization

gnuplot

Based in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 18

gnuplot accepts input files with an x,y pair on each line:

% cat plot.dat500 22550 14600 12650 13700 3750 7800 0850 7900 6950 31000 2

gnuplotBased in Part on Previous Lecture of J. Grefenstette

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gnuplot gnuplot can create PNG format files, which work in all

common browsers

# file hist.plt# usage: gnuplot hist.plt

set output "hist.png" # name of output file

set terminal png medium mono # type of output file = PNG

set size 0.8, 0.8 # adjusts size of text

unset key # do not display key

set xlabel "Length" # x-axis label

set ylabel "Sequences" # y-axis label

set title "Sequence Length Distribution"

set style fill solid 1.0 # make boxes black

set boxwidth 0.9 relative # leave small space between boxes

plot "plot.dat" with boxes # plot the data file

exit

19BINF 634 Fall 2013 - Lec 11 Visualization

gnuplot

Based in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization

20

Output

gnuplotBased in Part on Previous Lecture of J. Grefenstette

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## File: hist3.plt# usage: gnuplot hist3.plt## assumes data is stored in “myplot.dat"#set terminal png # type of output file = PNGset output "hist3date.png" # name of output fileset title "Hits Per Month" # title of graphset xlabel "Months" 0,-1 # x-axis label (down 1 character)set ylabel "Hits" # y-axis labelset size 0.75,0.75 # reduce graph sizeunset key # do not display keyset boxwidth 0.9 relative # leave small space between boxesset style fill solid 1.0 # make boxes solidset xrange [0:13] # control size of x-axis

# fine tune labels on x axisset xtics rotate ("Jan" 1, "Feb" 2, "Mar" 3, "Apr" 4, "May" 5,

"Jun" 6, "Jul" 7, "Aug" 8, "Sep" 9, "Oct" 10, "Nov" 11, "Dec" 12)

# plot the data file, creating "hist.png"plot “myplot.dat" with boxes

21BINF 634 Fall 2013 - Lec 11 Visualization

gnuplotBased in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 22

gnuplot data file

% cat myplot.dat1 232 53 24 205 116 337 78 319 7710 1811 6512 52

gnuplotBased in Part on Previous Lecture of J. Grefenstette

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BINF 634 Fall 2013 - Lec 11 Visualization 23

hist.png

gnuplotBased in Part on Previous Lecture of J. Grefenstette

Page 24: BINF 634 Fall 2013 - Lec 11 Visualization1 Plan for Tonight A synopsis of our progress through the course Lecture 11 Visualization in PERL Lab 2 Based

Getting the Chart on the Web2. In the wrapper, create the graphics file in the working directory3. Copy it to the images directory4. Display it using print img{src=>...}

use CGI qw(:standard);$ENV{PATH} = "/bin:/usr/bin:/usr/local/bin"; # for gnuplot ...

# change to working directorymkdir $dir or die "Can't create directory $dir\n";chdir $dir or die "Can't change to directory $dir\n";

# create the data file for plottingsystem "cp /userhomes/faculty/jsolka/binf634/visualization/plot.dat $dir";

# run the plotting programsystem "gnuplot /userhomes/faculty/jsolka/binf634/visualization/hist.plt";

# copy the output image to the web images directorysystem "cp hist.png /userhomes/faculty/jsolka/public_html/cgi-images/hist.png";

# display image file on the web pageprint img {src=>"/jsolka/cgi-images/hist.png"};

24BINF 634 Fall 2013 - Lec 11 Visualization

gnuplot

Based in Part on Previous Lecture of J. Grefenstette

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Perl Data Language (PDL)

BINF 634 Fall 2013 - Lec 11 Visualization 25

http://pdl.perl.org/

PDL

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A Few Discussions on PDL

The next several slides were adapted from the PDL tutorial by David Mertens at this URL

http://www.slideshare.net/dcmertens/p-lplot-talk

BINF 634 Fall 2013 - Lec 11 Visualization 26

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 27

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 28

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 29

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 30

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 31

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 32

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 33

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 34

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 35

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 36

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 37

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 38

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 39

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 40

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 41

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 42

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 43

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 44

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 45

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 46

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 47

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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BINF 634 Fall 2013 - Lec 11 Visualization 48

Adapted from David Mertens at this URLhttp://www.slideshare.net/dcmertens/p-lplot-talk

PDL

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More information Online documentation for GD::Graph

% perldoc GD::Graph

Online doc for gnuplot% gnuplot> help

Also see: http://www.gnuplot.info PLplot

http://plplot.sourceforge.net/examples.php PDL

http://pdl.perl.org/

49BINF 634 Fall 2013 - Lec 11 Visualization

Summary

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BINF 634 Fall 2013 - Lec 11 Visualization 50

Homework and Reminders

Program 4 due next week 11/26/12 Lab 2 due by next week No more quizzes No more programming assignments Only the final

Read Chapter 11 Exercise 11.1 and 11.2

Reminders