be nazo caine
TRANSCRIPT
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Preparation of Benzocaine by Acid-catalysed
Esterification of a Carboxylic acid and Alcohol(C9H11NO2 Mol.Wt. 165.2)
Theory:
When a carboxylic acid is heated with an alcohol in presence of mineral acid, e.g. sulphuric acid, hydrochloric
acid or hydrogen chloride gas, the hydrogen atom of the carboxyl group is replaced by an alkyl group in the
resulting product is called an ester. Hence, the reaction of a carboxylic acid with an alcohol to give an ester is
known as esterification. When ethanol is heated with p-Amino benzoic acid in presence of Conc. sulphuric aci
Benzocaine (Ethyl p- amino benzoate) forms. This esterification is Fischer esterification.
H2N + H2SO4 + H2O
p-Amino benzoic acid Ethanol Benzocaine(Ethyl p-amino benzoate)
Procedure:
Weigh and transfer 3.0g of p-Amino benzoic acid in a R.B. flask, then add 30ml of absolute ethanol. Shake we
to dissolve the solid. Add 3ml of sulphuric acid dropwise into the ethanol-amino benzoic acid solution. A so
precipitates out of the solution. Attach a condenser to the round bottom flask , and set up the apparatus for heati
under reflux. Connect the condenser to tap water supply. Heat the solution on a heating mantle under gentle reflu
for approximately 30 minutes. Start heating slowly. Adjust the heat to keep the solution refluxing, but not boili
violently. Refluxing is confirmed when liquid droplets form at the tip of the condenser where the condenser
attached to the round bottom flask.
If any solid precipitate remains after heating, take the condenser off of the round bottom flask and remove th
round bottom flask from the heating mantle. Cool the solution at room temperature for 3-5 minutes. Add 9ml absolute ethanol and 1.5ml of sulfuric acid (dropwise) to the round bottom flask containing the solution an
precipitate. Reattach the condenser to the round bottom flask, and resume heating under gentle reflux until all
the solid has dissolved. It is very important for this experiment to succeed that ALL of the solids dissolve duri
this period.
Once the solid is completely dissolved, continue heating the solution under gentle reflux for another 30 minute
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Remove the round bottom flask from the heat source and allow the reaction mixture to cool to room temperatu
(7-10 minutes). Fill a 250mL beaker with 60ml of water and pour the cooled reaction mixture into the beaker.
Basify the reaction mixture and water solution to a pH of about 8. To do this, calculate the approximate volume
20% aqueous sodium carbonate required to neutralize the total amount of sulfuric acid you used (Usually betwe
27-36 ml). Slowly pour the calculated amount of 20% aqueous sodium carbonate into the beaker containing treaction mixture and water solution. Be sure to add the sodium carbonate slowly as frothing occurs durin
neutralization . A solid should precipitate. This is crude benzocaine.
Filter the mixture. Then transfer the crude benzocaine into a 125ml conical flask. Add about 60ml of water, the
shake to mix properly. Heat the mixture to 60
C. Add minimum quantity of methanol (15-25ml) to dissolve the
precipitates . Do not add more methanol than required. Then remove the flask from the heating source and
allow the solution to cool to room temperature. It takes about 10 minutes. Cool the solution in an ice water bath
for 15 minutes to complete crystallization of the benzocaine (white or colourless crystals) , then filter. Allow to
dry the crystals. Finally calculate the yield and find out its m.p. (89.0 92)
C.
Benzocaine