bch 3033 general biochemistry flipped welcome to the fall-2013 inverted or flipped general...
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BCH 3033General Biochemistry Flipped
Welcome to the Fall-2013Inverted or Flipped General Biochemistry Course
The “Lectures” are OnlineYou Need to Gain Biochemical Knowledge BEFORE Class
Class Time = Quizzes + Problem Solving + Case Studies
CLASS TIME IS TO EXERCIZE YOUR KNOWLEDGE
Syllabus on Blackboard Learn
And at… http://www.fiu.edu/~bch3033
Advantages of Blackboard Learn: 1. The “Lectures” = Narrated Power Points
2. Case studies as pdf files.3. You can create your Discussion Groups4. Grade Book…keeps updating your grades
with each class…Clicker Scores and Exams..includes class statistics
You Need to get an iClicker…the graded part of class time begins next week.
What’s Involved ?
LEARNING BIOCHEM
The Standard 3 Credit Course generally needs 6-8 hours SERIOUS study/week
You will need to LEARN the “Lectures” and Solve End of Chapter Problems BEFORE Class Time
You will see that some “Lectures” are short, ~35 minutes, others are about an hour. BEFORE CLASS you will need to practice what you
learned from the “lecture” by doing end of chapter problems.
Study ? Gain Knowledge ?
What is the “trick”? DO IT BEFORE CLASS !!
BEFORE CLASS LEARNING to develop Deep Knowledge….
not shallow knowledge !
Study ? Gain Knowledge ?
What is the Deal? Each Flipped Class requires Mindful Study.
Flipped Classes: EXERCISE Your Knowledge IN CLASS
aClass
This Inverted or Flipped ClassAll the “Lectures” are on-line narrated PowerPoints…..
You need to LEARN the PowerPoints and DO Selected End of Chapter Problems BEFORE CLASS
Class time will be used doing “advanced homework”: problem solving using chemistry, math, and biochemical
concepts.
Every Class has a Graded Component = 1/3 of Total Grade, Class Grade comes from iClickers
The Syllabus is on Blackboard Learn
Answers and Short Solutions to EOC Problems start after page 1198 in the text.
Class Response System: iClickers
iClickers come in different forms
You can buy new or used iClickersfrom the bookstore or used ones from friends, older
models will work fine as long as the batteries are working.
or get an App for your Smart Phone, Tablet or Laptop
iClicker Registration
You need to REGISTER your iClicker: go to iClicker.com
You need: Your name, PantherID and the iClicker Serial Number (on the back of the iClicker)
If you get a used iClicker, make sure the Serial Number is clearly visible and not rubbed off.
General Biochemistry has 3 Parts
PART 1: Chemical Structure of: Proteins (amino acids), Polysaccharides (sugars), Nucleic Acids (nucleotides) and Lipids (glycerol and fatty acids, steroids, ceramides)…and chemical methods of their purification and their structure determination.
PART 2: Mechanisms: Ionic dissociation, pH, enzyme kinetics, thermodynamics, signaling mechanisms. This part integrates into Parts 1 and 3.
PART 3: Metabolic Pathways and Their Control. We will focus on Central Metabolism: Glycolysis, Gluconeogenesis, Pentose Phosphate Pathway, Citric Acid Cycle, Fatty Acid and Amino Acid catabolism and Electron Transport.
General Biochemistry has 3 Parts
PART 1: Chemical Structure of: Proteins (amino acids), Polysaccharides (sugars), Nucleic Acids (nucleotides) and Lipids (glycerol and fatty acids, steroids, ceramides)…and chemical methods of their purification and their structure determination.
PART 2: Mechanisms: Ionic dissociation, pH, enzyme kinetics, thermodynamics, signaling mechanisms. This part integrates into Parts 1 and 3.
PART 3: Metabolic Pathways and Their Control. We will focus on Central Metabolism: Glycolysis, Gluconeogenesis, Pentose Phosphate Pathway, Citric Acid Cycle, Fatty Acid and Amino Acid catabolism and Electron Transport.
Six kingdoms• Archaea• Bacteria• Protista• Fungi• Plantae• Animalia
Cellular organizationUnicellular prokaryoteUnicellular prokaryoteUnicellular eukaryoteUni- or Multicellular eukaryoteMulticellular eukaryoteMulticellular eukaryote
The Six Kingdoms of Life
Fractionation by Density
Molecules sediment based on mass + friction (shape)…measured in Svedbergs (a multidimensional unit)
OK, Lets Do Some Practice Clicker Questions
1. You have a solution of 4 nM bovine serum albumin. The concentration of bovine serum albumin is:
A. 4 x 10-6 M. B. 40 x 10-8 M. C. 0.4 x 10-10 M. D. 4 x 10-9 M.
OK, Lets Do Some Practice Clicker Questions
1. You have a solution of 4 nM bovine serum albumin. The concentration of bovine serum albumin is:
A. 4 x 10-6 M. B. 40 x 10-8 M. C. 0.4 x 10-10 M. D. 4 x 10-9 M.
m = milli 10-3
μ = micro 10-6
n = nano 10-9
p = pico 10-12
f = femto 10-15
a = atto 10-18
Lets Do Some Practice Clicker Questions
2. Membrane transport proteins are found in:A. Cell membrane. B. Endoplasmic reticulum membrane.C. Mitochondrial membrane. D. Nuclear MembraneE. All of the above.
Lets Do Some Practice Clicker Questions
3. Which pair below is wrong in showing oxidized/reduced forms of carbon.
A. R-COOH/R-CHO. B. R-CH2OH/R-CHO
C. R-CHO/R-CH3 D. R-CH=CH2/R-CH2-CH3
E. All are wrong.
Practice Graphing QuestionsHere is a pH titration of a purified but unknown organic molecule in
100mL of water. Examine in the titration carefully.
Graphing Practice Question
4. Which titration graph is the most meaningful?
A. 1. B. 2.
Graph 1 Graph 2
Same Data in Excel
0 100 200 300 400 500 6000
2
4
6
8
10
12
14pH Titration Unknown Compound
WaterUnk Cpd in Water
μL 2M KOH
pH
Clicker Question: What is going on? (back to General Chemistry !)
The unknown is a:a. strong base.b. strong acid.c. ester.d. aldehyde.e. none of these
Clicker Practice Questions
5. Water at Room Temperature is a(n):A. Acid. B. Organic compound. C. Metal.
D. Solid. E. None of these.
Clicker Practice Questions
6. 5 μmoles/mL isA. 5 x 10-6 M. B. 5 x 10-3M. C. 5 x 10-9M.D. 50 nM. E. none of these.
Clicker Practice Questions
7. Arrange in order of reduced oxidized forms of nitrogen.A. Amine/Amide/Nitro.B. Nitro/Amide/Amine.C. Nitro/Amine/Amide.D. Amide/Amine/Nitro.E. Amide/Nitro/Amine.
Where Are They
Here is the drug morphine. How many Hydrogen atoms does it have ?
A. 7. B. 19. C. 14. D. 18. E. 17.
Where Are They
Here is the drug morphine. How many Hydrogen atoms does it have ?
A. 7. B. 19. C. 14. D. 18. E. 17.
11
1
2
11
1
1 2
11
2
3
1
More Clicker Questions
9. “femto” is between:A. milli and nano. B. micro and pico.C. atto and pico. D. micro and macro.E. sad and happy.
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the protein lacR. What is the concentration of the protein in the cell?
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the protein lacR. What is the concentration of the protein in the cell?
Solution: Cell volume = 4/3 πr3 = 4/3 π (5 x 10-7m)3 = 5.24 x 10-19m3 = 5.24 x 10-16 L
8 molecules / (6.02 x 1023 molecules/mole) = 1.33 x 10-23 moles
1.33 x 10-23 moles / 5.24 x 10-16 L = 2.5 x 10-8 M or ?
More Clicker Questions
10. A spherical cell, 1 µm in diameter, has eight molecules of the protein lacR. What is the concentration of the protein in the cell?
What is 2.5 x 10-8 M?A. 25 nM. B. 0.025 mM. C. 2.5 µM.
Class Periods
Can Start with an iClicker Quiz on the Lecture and
End of Chapter Problems (EOC problems).
These are Graded Activities that are 1/3rd of your Grade!
Some classes will have a Case Study.
The Case Studies will NOT be on any Exam, but will have Graded iClicker Questions exercising what you learned
from Narrated Power Point and Interpreting Biochemical Data and solving Problems for that Case Study.
STUDY to LEARN before EACH CLASS1. Mindfully Examine (do not passively
listen) the Narrated PowerPoint. .. Mindfully test yourself on it to make sure you know this biochemistry…so you can use it in Class.
2. Do the practice End of Chapter Problems…be able to do them with the book closed….
3. Check out the Case Study for that day on Blackboard (some will not have a Case Study, most do).
The Course is in Blackboardand on the Web
http://www.fiu.edu/~bch3033
The Web version Lacks what Blackboard Has:1. The Lectures as MP4 files on FIU Media
Site.
2. Grade book…you can keep track of how you are doing.
3. and more.