B ZERO AFLA M1

The most rapid and cost effective ELISA test kit for aflatoxin M1

Aflatoxin M1

• Aflatoxin M1 is a toxic metabolite resulting from the

digestion of Aspergillus Flavus mycotoxin aflatoxin B1

in dairy animals; the main source of contamination are feed and cereals.

• Aflatoxin M1 is particularly toxic for liver and kidneys.

• Aflatoxin M1 is excreted in milk within 24 hours from

intake of contaminated feed. Clearance is reached within 1 and 4 days from contamination.

Product overview

• Assay principle: competitive enzyme immunoassay

• Application: raw milk

• Measuring range: 10 – 200 ng/l

• Detection limit: 10 ng/l aflatoxin M1 in milk

• Assay time: 30 minutes

• Sample preparation: none

• Limit of quantification: 20 ng/l(100% sensitivity)

• Mean intra-assay CV: 5%

• Mean inter-assay CV: 8%

The B ZERO format

The B ZERO format

• The use of just one standard and the absence of any replicate make B ZERO AFLA M1 the most cost effective solution for aflatoxin M1 screening.

• B ZERO brings together speed, ease of use, and reliability in a single ELISA kit.

Ideal for who implements few

samples per analysis as well as who has

high throughput needs!

About sample preparation

• Sample preparation is a fundamental step for any analysis; its complexity has a relevant incidence on the total time the analysis requires.

• For aflatoxin M1 analysis, traditional ELISA kits usually require to cool

the milk sample, to centrifuge it for approximately 10 minutes and to take away the fat layer.

Bottle-neck procedure when many samples have to be analysed

Additional source of error

How many samples can you centrifuge together?

How many vials do you need to defat your samples?

How much time do you need to prepare your milk samples?

Avoidingmilk manipulation

• TECNA B ZERO AFLA M1 is

the first ELISA kit for

aflatoxin M1 that brings

together the high quality

performances of

traditional ELISA tests and

strip-tests ease of use.

Thanks to the highly specific antibody and to the perfect in-matrix calibration B ZERO AFLA

M1 is the first ELISA kit

that does not require defatting to analyze

aflatoxin M1 in milk!

• Specific experiments were performed in order to evaluate the correlation

between results of analysis of defatted and not defatted milk on more than 60 samples

• No accuracy or precision statistical differences aroused when analyzing not defatted milk (correlation between defatted and not defatted milk was r2=0.96)

ng/L defatted milk samples

ng/L

not

def

atted

Performance assessment

Why B ZERO AFLA M1?

NO milk manipulation- Save on time and lab equipment- Reduce any source of error

Results in just 30 MINUTESNO calibration curve needed- Save on wells dedicated to calibrators

No need to run any replicate

Visit us at www.tecnalab.com!