automated diagnostics: tbdx project presentation
TRANSCRIPT
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TB‐dx Final Design
J. Bentley, K. Cooley, D. Crowe, M. Evans, L. Sehgal
University of Rochester
Department of Biomedical Engineering
05/06/2009
Compiled for Benjamin Castaneda and Fanny Casado by Automated
Diagnostics under the supervision of Edward Brown III, Ph.D.
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Outline Problem Statement
Customer Scenario
Standard of Care in Peru
Overall System Design
Division of Tasks
Design Process
Final Design
Future Prospects
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The Issue
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Medicine World
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Customer Scenario Hospital Nacional Dos de Mayo
in Lima, Peru
Current Situation in Peru
TB is a serious public health threat in Peru
44% of the population is below the poverty line
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Standard of Health Care in Peru Tuberculosis Diagnostic Procedure
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Image source: PUCPDigital image of Bacilloscopy
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Overall System Design
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TB-dx Setup NEEDS NEW PIC!!!
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User Interface Quick Summary of user interface…
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Pontificia Universidad Catolica del Peru Image processing and
detection of mycobacteria
Count bacilli
Future: Auto Focusing
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Image Processing Team from PUCP
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University of Rochester
Receive output # of bacilli from PUCP
Automate stage movement for 1 slide
Quantify/qualify focus problems
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Automated Diagnostics
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Experimental Results Conducted experiments to determine the sensitivity of
focus across a slide with 100X oil lens
Used parallel slices of rat femur and uneven human TB-free sputum samples to quantify
Attempted to move as many millimeters as possible until the sample was out of focus
On average, human samples had to be re-focused every millimeter
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Prototype Iteration #1
Flat slide bed Rubber band belts
Plastic motor housing
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Prototype Iteration #2
Multi-layered slide bed
Rubber band belts
Machined aluminum motor housing
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Final Design: TB-dx
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Final Design: TB-dx
Existing slide holder on Olympus CX31 microscope stage
Flat NBR‐Coated Nylon Fabric Belts
Machined aluminum motor housing
NEED PICTURE WITH NEW BELTS HERE
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Olympus CX31 Manual
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Future Prospects Multiple Slides
Automated Focus
Compatibility
Zeiss
Computer Free Product
Entire System Testing
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Carl Zeiss’ Primo Star iLED fluorescence microscope
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Thank You
• Ben Castaneda
• Fanny Casado
• Professor Ed Brown
•
• Art Salo
• Paul Osborne
• Professor Scott Seidman
• Professor Amy Lerner
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