autodisplay biotech gmbh - a novel approach to the expression of challenging enzymes
DESCRIPTION
If enzymes resemble a scientific or economic bottleneck, a unique expression system can help: Our proprietary cell surface display technologies MATE and Autodisplay combine the benefits of soluble enzymes and whole cell biocatalysts. Enzymes are naturally immobilized on the surface of gram negative bacteria. The reaction is facilitated on the outside of the cell – no transport across the membrane is needed. This means there are virtually no limitations regarding substrate or product (toxicity or size). We have broad expertise with especially challenging enzymes such as multimeric enzymes (e.g. nitrilases) or enzymes that require cofactors (e.g. P450 enzymes). Find out how Autodisplay Biotech's custom-tailored products and services can contribute to driving your R&D activities to the next level.TRANSCRIPT
THE FOLLOWING
QUESTIONS!
a s k
your-
s e l f
1
HAVE ENZYMES EVER
BEEN A LIMITING FACTOR IN
YOUR PROCESSES?
HAS INTRACELLULAR
EXPRESSION OF ENZYMES
BEEN A BOTTLENECK?
2
HAVE YOU EVER
WISHED, TO BE ABLE TO
RECOVER ENZYMES FROM
YOUR PROCESSES?
3
WAS IMMOBILISATION OF
ENZYMES DISCARDED BECAUSE
OF HIGH COSTS?
4
ONETHERE IS
OPTION
COMBINING THE POSITIVE
CHARACTERISTICS OF
free
enzymes
whole cell
biocatalysts
immo-
bilisation
WHILE RETAINING
MAXIMUM FLEXIBILITY
bacterial
surface
display
NATURAL IMMOBILISATION OF
ENZYMES THROUGH…
SP PASSENGER LINKER β-BARREL
IM
OM
PP
CP
OUR TECHNOLOGY…
IM
OM
PP
CP
SP PASSENGER LINKER β-BARREL
… IS A SELF-SUFFICIENT SYSTEM
WHAT ARE THE BENEFITS
simply
centrifuge or
filter
FOR A COST-EFFICIENT
PURIFICATION OF ENZYMES…
1
FOR THE RECOVERY OF ENZYMES
FROM A CONVERSION PROCESS…
2
simply
centrifuge or
filter
100%
#5#1
CYCLES
@24h each#2 #3 #4
CONVERSION
NITRILASE EXPERIMENTS PROVE:
RECOVERY IS SUCCESSFUL
SCHEMATIC DRAWING
70%
50%
30%
10%
UNLOCK ENHANCED ENZYME
STABILITY THROUGH…
3natural
immobilisation
smart
choice of host
organisms
ENABLE METABOLIZATION OF
ENZYME INHIBITORS THROUGH…
4
integrated
cleavage sites
(& HIS-tag)
RELEASE ENZYMES EXPRESSED
WITH AUTODISPLAY THROUGH…
5
resuspendent
cells
SUPERNATANT
HIGH CELL DENSITY
FERMENTATION
CONCENTRATED
SUPERNATANT
CONCENTRATION OF RELEASED
ENZYMES IS SIMPLE
FILTRATE1 2 N
HOW MANY
MOLECULES ARE LOCATED
ON THE SURFACE?
you
may
a sk
MOLECULES/CELL
100,000 200,000–
WE CAN HELP, WHEN
OTHER SYSTEMS FAIL
ESPECIALLY IN THE CASE OF
CHALLENGING ENZYMES
WHAT ARE
CHALLENGING
ENZYMES?
you
may
a sk
inclusion
bodies
multimeri-
sation
incorrect
folding
co-factors
lack of
stability
ALL ENZYMES WHICH ARE
DIFFICULT TO HANDLE, DUE TO…
+
ENZYME PROBLEM NAME THE ENZYME GENE SEQUENCE
GACTTCGAAGTC
TTTAAGAATGACT
TCGAAGTCTTTAT
PLASMIDHOST
ORGANISM
gram –
WHOLE CELL
BIOCATALYST
1 2
3
4
WE ARE DEDICATED TO SOLVE
BIOCATALYTIC BOTTLECKS
enzyme
recovery/
re-use
enzyme
stability
enzyme
purification
enzyme
portfolio
enzyme
costs
sales and
profitability
RECAP: VALUE PROPOSITION
TALK TO US, IF YOU
WANT TO KNOW MORE