attila szautner...attila szautner tma technology marked area matching area on the donor block...
TRANSCRIPT
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Attila Szautner
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TMA technology
Marked area
Matching area on the donor block
Recipient block
1.
2.
3.
Embedding
4.
5.
Scoring
Glass slide
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TMA technology
Marked area
Matching area on the donor block
Recipient block
1.
2.
3.
Embedding
4.
5.
Scoring
Glass slide
![Page 4: Attila Szautner...Attila Szautner TMA technology Marked area Matching area on the donor block Recipient block 1. 2. 3. Embedding 4. 5. Scoring Glass slide TMA technology Marked area](https://reader036.vdocuments.us/reader036/viewer/2022062609/60faa3e2da715c68d01c4c49/html5/thumbnails/4.jpg)
TMA technology
Marked area
Matching area on the donor block
Recipient block
1.
2.
3.
Embedding
4.
5.
Scoring
Glass slide
![Page 5: Attila Szautner...Attila Szautner TMA technology Marked area Matching area on the donor block Recipient block 1. 2. 3. Embedding 4. 5. Scoring Glass slide TMA technology Marked area](https://reader036.vdocuments.us/reader036/viewer/2022062609/60faa3e2da715c68d01c4c49/html5/thumbnails/5.jpg)
TMA technology
Marked area
Matching area on the donor block
Recipient block
1.
2.
3.
Embedding
4.
5.
Scoring
Glass slide
IHC, FISH
![Page 6: Attila Szautner...Attila Szautner TMA technology Marked area Matching area on the donor block Recipient block 1. 2. 3. Embedding 4. 5. Scoring Glass slide TMA technology Marked area](https://reader036.vdocuments.us/reader036/viewer/2022062609/60faa3e2da715c68d01c4c49/html5/thumbnails/6.jpg)
TMA technology
Marked area
Matching area on the donor block
Recipient block
1.
2.
3.
Embedding
4.
5.
Scoring
Glass slide
IHC, FISH
![Page 7: Attila Szautner...Attila Szautner TMA technology Marked area Matching area on the donor block Recipient block 1. 2. 3. Embedding 4. 5. Scoring Glass slide TMA technology Marked area](https://reader036.vdocuments.us/reader036/viewer/2022062609/60faa3e2da715c68d01c4c49/html5/thumbnails/7.jpg)
Advantages
• Save:
– Reagent
– Time
– Tissue
– Workforce
• Standardized laboratory conditions
• Central data handling
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Case Selection
• Collecting donor blocks
• Preparing clinical data
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Analysis of digital slides, Creating markers
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Workflow, Evaluation of TMA slides
TMA BLOCK CREATION
Case Center
SCANNING THE TMA SLIDES
EVALUATION & ANALYSIS
Slide preparation tools
DNA isolation, DNA amplification, DNA sequencing
PCR extraction
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TMA Module Software
• The TMA Module deals with simultaneous handling of many slides and cores
• Multi-user option (Consolidator function)
• Filter the spots in the gallery by different parameters (unscored – rescored)
• Statistical analysis
• Simple or Combined scoring scheme
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Workflow steps of the TMA Module
• Preparing the Excel sheet for the TMA project
• Spot finding
• Scoring and managing slides
• Export data and images
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Block1
Block3
Block2
Preparing the Excel sheet for TMA project
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Scoring and managing slides
• Combined Scoring
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Scoring and managing slides, Gallery
• Scoring
spots
• Ordering
spots
• Filtering
spots
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TMA Grand Master
• 72 block capacity:
12 recipient blocks, 60 donor blocks
• 4 donor punch and drill bit sizes:
0.6, 1, 1.5, 2 mm
• 2 digital cameras (1 for label and 1 for block view)
• Automated block and tool height measurement by sensor
• Separately controlled puncher and drill bit
• Parallel block feeding, drilling, punching and image capture
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TMA Grand Master
• Faster movements and faster workflow
• Computer-aided tasks:
– Recipient block layout design
– Assign a digitized slide to a donor block
– Data export to Excel
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TMA Grand Master
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+ =
Jpg image overlay
• Set the transparency near the block image
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Barcode reading
• Auto Barcode button
• 1D Barcode Types – Code 128, Code 93, EAN8, EAN13, UPC_A
• 2D Barcode Types – Datamatrix, Maxicode, Micropdf417, QRcode
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PCR in Grand Master
• Dedicated position
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Cleaning block
• Set the cleaning block
• Clean before each PCR extraction