assay development and drug repurposing core
TRANSCRIPT
Assay Development and Drug Repurposing Core
Drug Discovery Forum
Tom Dexheimer, Ph.D.Manager, MSU Assay Development and Drug Repurposing Core
May 14, 2015
MSU Assay Development and Drug Repurposing Core
• To provide equipment and expertise for high-throughput screening techniques to MSU researchers
• To provide MSU researchers with access to cost-effective chemical library screening for identification of molecules to further interrogate particular biological processes or potential leads for drug development (or drug repurposing)
• To promote a training environment that will expose students to the techniques of high-throughput assay design, development, and HTS screen execution
• To provide assistance and/or the generation of preliminary data for grant/funding opportunities
Goals of the ADDRC
Equipment – Liquid handling
Multidrop 384 Reagent Dispenser
• Peristaltic pump based cassette dispenser • Dispense 96- and 384-well plates• 5 to 395 mL volume range (5 mL
increments)
EL406 Microplate Washer Dispenser
• Peristaltic pump based cassette dispenser
• 1 mL and 5 mL cassettes• 96-well washer module• Syringe pumps dispensers• Variable dispense and
aspiration rates
Biomek FX Laboratory Automation Workstation• Automated/programmable robotic dual arm
system with 96-multichannel pipettor and Span-8 pipettor (8 individually controlled channels)
• Equipped with 384-pin tool• Small volume plate to plate transfers (i.e. compound plate to assay plate)• Volume range - 100 nL to 200 nL
Cytation 3 Cell Imaging Reader
Equipment – Readers
• Automated digital microscopy• Objectives: 4x and 20x (currently available)• Brightfield, 365 nm, 465 nm, 590 nm• Temperature and CO2/O2 control
Synergy Neo Multi-mode Reader• Combines filter-based optical system and monochromator optics,
providing both excellent performance and assay versatility• Detection modes: Fluorescence, time-resolved fluorescence,
fluorescence polarization, Alpha, luminescence, UV-visible absorbance
• Multi-plate stacker (30 plates)
Functional Drug Screening System FDSS/µCELL• Kinetic plate reader with an
integrated dispensing head and imaging-based detector
• High-speed digital CCD camera• 0.5 s to 100 s/data point • 480 nm /540 nm• 96- or 384-well compatible• Ca2+, cAMP, membrane
potential, etc.
Chemical Libraries and Informatics
GREENScreen• Integrated compound management and
high throughput screening data storage and analysis system
Chemical Libraries• 6571 compounds
MSU Drug Discovery
Drug Discovery listserv ([email protected])• Bring together researchers from all disciplines at MSU who are interested
or involved in drug discovery and development
Drug Discovery Seminar Series• Fridays from 9:00 to 10:00 AM in B448 Life Science Building
• http://phmtox.msu.edu/core-facilities/Assay/DDIG.htm
Michigan Drug Discovery Symposium
• SAVE THE DATE September 9th-10th
Contact InformationTom Dexheimer, Ph.D. B411 Life Science BuildingEmail: [email protected]: 517-884-4998
*The ADDRC facility was established through the generous support of the Vice President for Research and Graduate Studies, the Pharmacology and Toxicology Department, CNS, COM, CHM, CVM, and AgBioResearch.