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8. Appendix

8.1. Sequences of mouse and human VITO-1/2 genes and their alignments

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Fig. 8.1.1. Coding region of the mouse VITO-1 gene displayed as nucleotide and

amino acid sequences (DNAStar software).

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Fig. 8.1.2. Coding region of the human VITO-1 gene displayed as nucleotide and

amino acid sequences (DNAStar software).

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Fig. 8.1.3. Coding region of the mouse VITO-2 gene displayed as nucleotide and

amino acid sequences (DNAStar software).

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Fig. 8.1.4. Coding region of the human VITO-2 gene displayed as nucleotide and

amino acid sequences (DNAStar software).

Fig. 8.1.5. Alignment between mouse and human VITO-1 genes on the protein level

using the Clustal W method.

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Fig. 8.1.6. Alignment between mouse and human VITO-2 genes on the DNA level

using the Clustal W method.

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Fig. 8.1.7. Alignment between mouse and human VITO-1/2 genes on the protein

level using the Clustal W method.

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8.2. Curriculum Vitae

DATE OF BIRTH: 21 September 1976

STATUS: married

NATIONALITY: Polish

EDUCATION:

1995-2000 Student of Adam Mickiewicz University of Poznań, Faculty

of Chemistry, Bioorganic Chemistry Specialization (G.P.A.:

4,5/5,0); Master of Science Thesis: Modified bases in nucleic

acids. Kinetin – properties and reactions (5,0/5,0)

Major: Chemistry

Minor: Molecular Biology

RESEARCH EXPERIENCE:

1997-1999 volunteer work in Biochemistry of tRNA Laboratory at the

Institute of Bioorganic Chemistry, Polish Academy of Sciences.

1999-2000 preparation of the Master of Science Diploma at the

Institute of Bioorganic Chemistry, Polish Academy of

Sciences.

2000-2001 PhD student at the Institute of Bioorganic Chemistry Polish

Academy of Sciences - not finished.

XII 2001 – I.2005 PhD student at the Martin Luther University Halle -

Wittenberg, Medicine Faculty, Institute of Physiological

Chemistry

II-2005-VI-2005 PhD student at the Martin Luther University Halle -

Wittenberg, Medicine Faculty, Institute of Physiological

Chemistry; Max-Planck Institute of Heart and Lung Research –

Bad Nauheim.

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VII. 2005 – VI. 2006 PhD student at the University Klinik Giessen – Marburg, Max-

Planck Institute of Heart and Lung Research – Bad Nauheim.

VII- 2006 PhD student at the Max-Planck Institute of Heart and Lung

Research – Bad Nauheim.

CONFERENCES AND COURSES

1. Polish School of Chemistry organized by Academic Federation of Chemistry

Students:

5th

Polish School of Chemistry, Murzasichle (1996), poster: “New faces of

fullerens” Mielcarek M., Ozdowy P.

6th

Polish School of Chemistry, Szklarska Poreba (1997), lecture: ”Oxygen –

friend or enemy” Mielcarek M.

7th

Polish School of Chemistry, Zajaczkowo (1997), lecture: “N6-furfuryladenine

as new modified bases of DNA” Mielcarek M., Barciszewski J.

9th

Polish School of Chemistry, Smrek (1998), lecture: “Application of high

pressure in chemistry and biology” Mielcarek M., Barciszewski J.

11th

Polish School of Chemistry, Szklarska Poreba (1999), lecture: “A new

methods in protein biosynthesis in vivo and their application in biotechnology

and medicine” Mielcarek M., Jarmolowski A., poster: “A molecular mechanism

of kinetin actions” Mielcarek M., Clark, B.F.C., Barciszewski J.

12th

Polish School of Chemistry, Puławy (2000) lecture: “Biological catalyst of

Diels-Alder reaction” Mielcarek M., Barciszewski J., poster: “Kinetin prevents

tRNA cleavage with OH radicals” Mielcarek M., Clark, B.F.C., Barciszewski J.

2. I Congress Polish Society of Cosmetic Chemists “Science for beauty”, Warsaw,

Poland (1998), poster: “A possibility of kinetin application in cosmoceutical

industries” Mielcarek, M., Barciszewski, J.

3. I Congress of Biotechnology, Wroclaw, Poland (1998), poster: “Kinetin –

biological properties and commercial applications” Mielcarek, M.,

Barciszewski, J., Clark, B.F.C.

4. 18th

tRNA Workshop “tRNA 2000” Cambridge, UK (2000), poster: “Kinetin

inhibits tRNA degradation with hydroxyl radicals” Mielcarek, M., Clark,

B.F.C., Barciszewski, J.

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5. Polish-American Conference Physico-Chemical Methods in Biotechnology and

Material Science, Poznan, Poland (2000), poster: “Kinetin inhibits tRNA

degradation with hydroxyl radicals” Mielcarek, M., Barciszewski, J.

6. Course: RNA: Biochemistry & Biotechnology FEBS, Poznan, Poland (1998)

7. Forum “Horizon Chimie”, Paris, France (1999) organized by ECPM, ENSCP,

ENSIC, ESPCI

8. International Conference “Nucleic Acids and Their Constituents: Chemical

Evolution underlying Biological Evolution” Poznan, Poland (1998)

9. International Conference “Molecular Architecture of Evolution: Primary and

Secondary Determinants” Poznan, Poland (2000), poster: “Identification kinetin

in human urine” Mielcarek, M., Barciszewski, J.

PUBLICATIONS:

1. Mielcarek, M., Barciszewski, J., (1998) Chemical modification of DNA (in

Polish), in: On the frontier of Chemistry and Biology, Koroniak, H.,

Barciszewski, J., Markiewicz, W.T., Ziemnicki, K., Eds., A. Mickiewicz

University Press, Poznan, Poland, pp 217-242

2. Barciszewski, J., Mielcarek, M., Barciszewska, M.Z., (1999) Modified

nucleosides of nucleic acids. Occurrence and properties of kinetin (in Polish), in:

On the frontier of Chemistry and Biology, Koroniak, H., Barciszewski, J., Eds.,

A. Mickiewicz University Press, Poznan, Poland, pp 77-111

3. Barciszewski, J., Mielcarek., M., Stobiecki, M., Siboska, G., Clark, B.F.C.,

(2000) Identification of 6-furfuryladenine (kinetin) in human urine. Biochem.

Biophys. Res. Commun. 279, 69-73

4. Mielcarek, M., Barciszewska, M.Z., Salanski, P., Stobiecki, M., Jurczak, J.,

Barciszewski, J., (2002) Native transfer RNA catalyzes Diels-Alder reaction.

Biochem. Biophys. Res. Commun. 294, 145-148

AFFILIATIONS:

Member of Federation of Polish Biochemical Society (FEBS Constituent Society)

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8.3. Publications and scientific activity in congresses during PhD studies

8.3.1. Publications

1. Mielcarek, M., Gunther, S., Kruger, M., Braun, T., (2002) Vito-1, a novel

vestigial related protein is predominantly expressed in the skeletal muscle

lineage. Mech. Dev. 119S, S269-S274

2. Gunther, S.*, Mielcarek, M.*, Kruger, M., Braun, T., (2004) Vito-1 is an

essential cofactor of TEF-1 dependent muscle specific gene regulation. Nucl.

Acid Res., 32, 791-802.

3. Mielcarek, M., Piotrowska, I., Kostin, S., Schneider, A., Gossler, A., Braun, T.,

VITO-2, a novel member of VITO family genes depends on Myf-5 during early

embryogenesis. (manuscript in preparation)

4. Mielcarek, M., Kostin, S., Piotrowska, I., Braun, T., Distinct role of VITO

genes in muscle formation. (manuscript in preparation)

8.3.2. Presentation

I Myores Annual Meting Rome, Italy (2005), poster: “Vito proteins are essential

new cofactors of the muscle regulatory programme” Mielcarek, M., Braun, T.,