antioxidant and sensorial properties of acacia …sensorial properties introduction honey is a...

APTEFF, 43, 1-342 (2012) UDC: 638.165+634.22]:542.943’78:543.92 DOI: 10.2298/APT1243293T BIBLID: 1450-7188 (2012) 43, 293-304

Original scientific paper

293

ANTIOXIDANT AND SENSORIAL PROPERTIES OF ACACIA HONEY SUPPLEMENTED WITH PRUNES

Vesna T. Tumbas*a, Jelena J. Vulića, Jasna M. Čanadanović-Bruneta, Sonja M. Djilasa,

Gordana S. Ćetković a, Slađana S. Stajčić a, Dubravka I. Štajner b and Boris M. Popovićb

a

University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia b

University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia The changes in total phenol and flavonoid content, as well as antioxidant activity was monitored in acacia honey supplemented with prunes in 20, 30 and 40% mass concentra-tions. The total phenolic content increased by 2.5 times (from 16.18 to 41.64 mg GAE/100 g) with increasing concentration of prunes in honey, while the increase in fla-vonoid content was even higher, approximately 11.5-fold (from 2.65 to 30.86 mg RE/100 g). The addition of prunes also improved th eantioxidant activity of acacia honey. The honey samples with highest content of prunes, 40%, exhibited the best antioxidant activity measured by hydroxyl radical sacvenging assay (EC50

•OH=4.56 mg/ml), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay (EC50

DPPH=16.48 mg/ml), and re-ducing power (EC50

RP=81.17 mg/ml). Judging from the high correlation coefficients, ran-ging from 0.771 to 0.947 for total phenolics, and from 0.862 to 0.993 for total flavonoids, it is obvious that these compounds were associated with the antioxidant mechanisms. On the other hand, sensorial properties of supplemented honeys were lower than that of pure acacia honey, where flavor of supplemented honey was the least affected. Our results in-dicate that the supplementation of honey with prunes improves antioxidant activity of ho-ney by enriching the phenolic composition, with slight modifications in sensorial charac-teristics. KEY WORDS: Acacia honey, prunes, phenolics and flavonoids, antioxidant activity,

sensorial properties

INTRODUCTION

Honey is a natural sweet substance that bees produce by transforming flower nectar or other sweet secretions of plants (1). As an easily assimilable food, honey makes a valuable nutritive product for children, athletes and convalescents (2). It was reported that honey contains about 200 substances (3). It is essentially a con-centrated aqueous solution of inverted sugar, but it also contains a very complex mixture

* Corresponding author: Vesna T. Tumbas, Faculty of Technology, University of Novi Sad, Bulevar cara

Lazara 1, 21000 Novi Sad, Serbia, e-mail: [email protected]



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of other saccharides, enzymes, amino and organic acids, polyphenols, carotenoid-like substances, Maillard reaction products, vitamins and minerals (4). Honey and other bee products have health-promoting properties which make them usable in pharmacy and medicine, both as drug components, prophylactic agents and diet supplements. Honey activity in gastrointestinal disorders has been reported, providing gastric protection against acute and chronic lesions (5). Also, honey antimicrobial proper-ties have been known for thousands of years and have been attributed to phenolic com-pounds derived directly from the honey (6). It was reported that the composition and antioxidant activity of honey depend on the floral source used to collect nectar by honeybee, seasonal and climatic factors. Also, pro-cessing may have an effect on honey composition and antioxidant activity (7, 8). The components in honey responsible for its antioxidative effect are flavonoids, phenolic acids, ascorbic acid, catalase, peroxidase, carotenoids, and products of the Mail-lard reaction. The content of these components varies widely according to the floral and geographical origin of honey (8, 9). Serbia has a very long tradition of beekeeping. Its favourable climate, good geogra-phical conditions and a variety of botanical species provide a great potential for the de-velopment of apiculture (10). The most common unifloral honeys are the acacia (Robinia pseudoacacia), sunflower (Helianthus annuus) and linden (Tilia cordata) honey. In order to expand the range of bee products taking into account the interest and satis-faction of the consumers, new technological solutions are constantly being sought. One method is to enrich the honey with some fruits which contain high-valuable organic com-pounds such as phenolics. In view of this the purpose of the present study was to determine the total phenolic and flavonoid content, as well as antioxidant activity of acacia honey supplemented with prunes, by three different assays, hydroxyl and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays and reducing power. Besides, a sensory analysis of the supplemented honey was also performed.

EXPERIMENTAL

Chemicals and instruments The chemicals used for these investigations were Folin-Ciocalteu reagent (Fluka Che-mical Co., Buchs, Switzerland), trichloroacetic acid, 2,2-diphenyl-1-pichrylhydrazyl (DPPH), 5,5-dimethyl-1-pyroline-N-oxide (DMPO), rutin and gallic acid (Sigma Chemi-cal Co., St. Louis, Mo, USA). All other chemicals and reagents were of the highest ana-lytical grade, obtained from J.T. Baker (Deventer, Holland). The total phenolic, flavonoid, DPPH free radical scavenging assay and reducing po-wer were determined using a UV-1800 spectrophotometer (Schimadzu, Kyoto, Japan), while the antioxidant activity against reactive hydroxyl radicals was evaluated by elec-tron spin resonance (ESR) spectroscopy (Bruker 300E ESR spectrometer, Rheinstetten, Germany).



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Honey and prunes samples The honey sample (AH), monofloral honey form of acacia (Rudnik region) (obtained during 2009 from the honeybee farm, Simonović, Beograd) was supplemented with Stan-ley variety prunes (Blace region) (obtained from the producer Tehno-Božići, Šabac). Pru-nes were cut into four pieces and added to the acacia honey in mass concentrations of 20% (AH20), 30% (AH30) and 40 % (AH40).

Total phenolic content The total phenolics were determined spectrophotometrically by the Folin-Ciocalteu method (11). The content of total phenolics was expressed as mg of gallic acid equiva-lents per 100 g of honey sample (mg GAE/100 g).

Total flavonoid content Total flavonoids were measured by the aluminium chloride spectrophotometric assay (12). Total flavonoid content was expressed as mg of rutin equivalents per 100 g of honey sample (mg RE/100 g).

Hydroxyl radical scavenging activity

Hydroxyl radicals (•OH) were generated in the Fenton reaction system obtained by mixing 0.2 ml of 112 mM DMPO, 0.2 ml of H2O, 0.2 ml of 2 mM H2O2, and 0.2 ml of 0.3 mM Fe2+ (control) (13). The influence of honey samples, at the range of concentrati-ons 5.0-25.0 mg/ml, on the formation and stabilization of hydroxyl radicals was investi-gated by ESR spin trapping method. The ESR spectra were recorded after 5 min, with the following spectrometer settings: field modulation 100 kHz, modulation amplitude 0.226 G, receiver gain 5 x105, time constant 80.72 ms, conversion time 327.68 ms, center field 3440.00 G, sweep width 100.00 G, x-band frequency 9.64 GHz, power 20 mW and tem-perature 23°C. The SA•

OH value of the honey samples was defined as:

SA•OH (%)= 100 × (h0 – hx) / h0

where h0 and hx are the heights of the second peak in the ESR spectrum of DMPO-OH spin adduct of the control and the samples, respectively.

DPPH free radical scavenging assay The scavenging activity of honey samples was determined spectrophotometrically using the modified DPPH method (14). Briefly, honey samples were dissolved in metha-nol, and 1.5 ml of each sample or 1.5 ml of methanol (blank) was mixed with 3 ml of DPPH in methanol (0.02 mg/ml). The range of investigated concentrations was 0.33-166.67 mg/ml. The mixtures were left for 15 min at room temperature and then the ab-sorbances was measured at 517 nm against reference mixtures that were prepared in the



296

similar manner, by replacing the DPPH solution with methanol. The capability to scaven-ge the DPPH radicals, DPPH scavenging activity (SA), was calculated using the follow-ing equation:

SADPPH• (%) = (A0 – Ax)/A0 × 100

where A0 is the absorbance of the blank and Ax is the absorbance of the sample.

Reducing power

The reducing power of honey samples was determined by the method of Oyaizu (15). For this puropse, the solution of honey samples (10–120 mg) in 1 ml of distilled water or 1 ml of distilled water (blank) was mixed with 1 ml of phosphate buffer (pH 6.6) and 1 ml of 1% potassium ferricyanide K3[Fe(CN)6]. The mixture was incubated at 50°C for 20 min and then rapidly cooled. Following this, 1 ml of trichloroacetic acid (10%) was ad-ded and the mixture was then centrifuged at 3000 rpm for 10 min. An aliquot (2 ml) of the upper layer, mixed with 2 ml of distilled water and 0.4 ml of 0.1% FeCl3, was left to stand for 10 min. The absorbance of the mixture was measured at 700 nm against the blank.

Sensory analysis

The main sensory attributes evaluated in the analysis were: color, viscosity, aroma, and flavor of honey samples, according to the Regulation on quality evaluation of bee products in Novi Sad fair (16). The sensory analysis were carried out by means of analy-tical descriptive analysis method, based on points (grades) from 0 (unacceptable product) to 3 (optimal quality level). The panel consisted of 5 expert descriptors.

Statistical analysis

All analyses were run in triplicate and the results were expressed as means ± standard deviation (SD). Statistical analyses were done by using Origin 7.0 SRO software package (OriginLab Corporation, Northampton, MA, USA, 1991–2002) and Microsoft Office Ex-cel 2007 software. Significant differences were calculated by ANOVA test followed by the least significant difference (LSD) test (p ≤ 0.05).

RESULTS AND DISCUSSION Supplemented acacia honey samples, AH20, AH30 and AH40, were subjected to spectrophotometric analysis of total phenolic and flavonoid contents. The results were compared to the contents in pure acacia honey, AH, obtained in our previous study (17) and presented in Figure 1.

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297

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298

In the recent years, there has been an increasing interest in the determination of the antioxidant activity of honey. Beretta et al. (20) have reported that it is necessary to use a combination of antioxidant tests, comparative analyses and statistical evaluation to deter-mine the antioxidant behavior of honey. Therefore, we used three different antioxidant tests to assess the activity of acacia honey samples. In our experiments, the decrease of •OH radical concentration, generated via Fenton reaction, was monitored using ESR spec-troscopy, and the decrease in the DPPH radical concentration was monitored spectropho-tometrically. Reducing power, another spectrophotometrical test, was used as a signifi-cant indicator of honey potential antioxidant activity. Antioxidant activities were expres-sed as EC50

•OH and EC50DPPH• value (the amount of antioxidant necessary to decrease the

initial concentration of hydroxyl or DPPH radicals by 50%) and EC50RP value (the effec-

tive concentration assigned at 0.5 value of absorption) and presented in Table 1.

Table 1. EC50 values of pure (AH) and supplemented acacia honeys (AH20, AH30 and AH40) measured by three antioxidant assays

Honey sample EC50

•OH (mg/ml) EC50DPPH• (mg/ml) EC50

RP (mg/ml) AH 19.14 0.76 164.09 7.38* 100.80 4.54*

AH20 16.89 0.54 24.48 1.05 96.75 3.82 AH30 15.89 0.63 18.65 0.84 92.13 4.25 AH40 4.56 0.18 16.48 0.69 81.17 3.95

* Taken form Savatović et al. (17) The results of all three antioxidant tests presented the same trend. The supplemented honeys, AH20, AH30 and AH40, exhibited higher antioxidant activity than pure honey, AH. The antioxidant activity increased with increasing the concentration of prunes in the honey. The greatest increase of the antioxidant activity was noted for the DPPH free radicals, where EC50

DPPH• value decreased 10 times compared to pure honey. The result of the AH antioxidant acitivity was in agreement with Meda et al. (19). It has been shown that dried plums have one of the highest ORAC values (5770) out of a group of 22 fruits and vegetables studied, and that phenolic compounds appear to be the main contributors to their antioxidant capacity (27). Many studies have shown that the antioxidant activity is strongly correlated with the content of total phenolics (7, 20, 8, 19). Gheldof et al. (4) stated that phenolic compounds significantly contribute to the antioxidant activity of honey. Also, the authors suggested that the antioxidant activity appeared to be a result of the combined activity of the honey phenolics, peptides, organic acids, enzymes and Maillard reaction products. For the correlation analysis, the EC50 values were transformed into their reciprocal values (1/EC50). Table 2 shows Pearson’s correlation coefficients between the analyzed antioxidant actvities of acacia honeys and composition variables. Our results confirm that phenolic compounds in general, and especially flavonoids, seem to be dominant compounds in honey-participating antioxidant reactions. The high-est correlation coefficient of 0.993 reveals a very good correlation between the flavonoid contents and reducing power of analyzed honey samples.



299

Table 2. Correlation matrix (Pearson’s correlation coefficients) between the composition variables and the antioxidant activities of pure and supplemented acacia honeys

Variable Total

flavonoids Total

phenolics 1/EC50

RP 1/EC50

DPPH• 1/EC50•OH

1/EC50•OH 0.895 0.771 0.928 0.605 -

1/EC50DPPH 0.862 0.921 0.851 -

1/EC50RP 0.993 0.947 -

Total phenolics 0.974 - Total flavonoids -

The relation between the three methods for the determination of antioxidant activity, and between the results of total phenolics and total flavonoids, was also good (r was from 0.605 to 0.974). Cimpoiu et al. (28) found a significant correlation (r = 0.9928) between the antioxidant activities determined by the DPPH and ABTS assays, suggesting that these two assays are almost comparable and interchangeable in the case of honey, when an evaluation of antioxidant activity is required. In our study, the antioxidant assays cor-related very well except for the correlation between the DPPH and hydroxyl radical assays, which was lower, but still good enough (Table 2). It is assumed that honeys contain compounds that can quench hydroxyl radicals also by additional reactions. Ghel-dof and Engeseth (8) found that the antioxidant capacities of honeys, evaluated by the oxygen radical absorbance capacity (ORAC) assay, showed a linear dependence on the total phenolics content. Zalibera et al. (29) investigated radical-scavenging capacities of 15 Slovak honeys using ESR and cation radical of ABTS (2,20-azino-bis(3-ethylben-zothiazoline-6-sulfonate) diammonium salt), DPPH and hydroxyl radicals generated by the photochemical decomposition of hydrogen peroxide. They found good ABTS•+ and DPPH free radical scavenging capacity of tested honeys, and that this capacity correlated well with the phenolics content. However, no correlation was found between the moni-tored ability to scavenge hydroxyl radicals and either phenolics content. Beretta et al. (20) found that the close interdependence between the phenolics content, FRAP, DPPH, and ORAC indicates that the antioxidant capacity of the honeys is due to their phenolic constituents, which are able to interact with Mo(VI) and Fe(III) with a H/e− transferring mechanism, and less to other chemical entities. Honey samples with and without prunes were evaluated by a 5-member trained expert descriptive attribute sensory panel. The samples were evaluated for color, viscosity, aro-ma and flavor. Samples were scored using the 0 to 3 intensity scale and the results are presented in Figure 2. The consumer evaluations of acacia honey samples indicated that pure honey, AH, exhibited best sensorial properties, while the addition of prunes affected viscosity at the highest level, and only slightly affected the flavor (3.0, 2.6 and 2.5 for AH20, AH30 and AH40, respectively) and aroma (2.0, 2.6 and 2.0 for AH20, AH30 and AH40, respective-ly) of the honey. However, the color of the supplemented honeys was rated lower, related to the changes in pale yellow color of pure acacia honey due to the dark brown pigmen-tation of the dried plum (2.0, 2.6 and 2.0 for AH20, AH30 and AH40, respectively).

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ONCLUSION

supplemented witplemented honeys

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542.943’78:543.92 (2012) 43, 293-304 nal scientific paper

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301

bioactive compounds, originating from honey itself and also from the added prunes. Ex-perimental results and statistical analysis show that these phytochemicals increase the honey’s antioxidant activity but also slightly affect sensorial properties. Considering the-se findings, the content of prunes could be optimized to obtain the best combination of the antioxidant and sensorial properties of the product. Also, the future studies can be fo-cused on the identification and quantification of phenolics and other compounds present in the supplemented honeys, and revealing other biological activities of these products in respect of health benefits that make these honeys a consumer-valuable product. This pro-duct can be recommended to complete other polyphenol sources such as vegetables and fruits.

Acknowledgement

This research is part of the Project No. 114-451-2093/2011-03, which is financially supported by the Provincial Secretariat for Science and Technological Development of the Autonomous Province of Vojvodina, Republic of Serbia.

REFERENCES

1. Lazarević, K., Andrić, F., Trifković, J., Tešić, Ž., Milojković-Opsenica, D.: Characte-risation of Serbian unifloral honeys according to their physicochemical parameters. Food Chem. 132 (2012) 2060-2064.

2. Socha, R., Juszczak, L., Pietrzyk, S., Fortuna, T.: Antioxidant activity and phenolic composition of herbhoneys. Food Chem. 113 (2009) 568-574.

3. Ferreira, I.C.F.R., Aires, E., Barreira, J.C.M., Estevinho, L.M.: Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem. 114 (2009) 1438-1443.

4. Gheldof, N., Wang, X.-H., Engeseth, N. J.: Identification and quantification of anti-oxidant components of honeys from various floral sources. J. Agric. Food Chem. 50 (2002) 5870-5877.

5. Ali, A.T.M.M.: Natural honey exerts its protective effects against ethanolinduced gas-tric lesions in rats by preventing depletion of glandular nonprotein sulfhydryls. Trop. Gastr. 16 (1995) 18-26.

6. Lee, H., Churey, J.J., Worobo, R.W.: Antimicrobial activity of bacterial isolates from different floral sources of honey. Int. J. Food Microb. 126 (2008) 240-244.

7. Al-Mamary, M., Al-Meeri, A., Al-Habori, M.: Antioxidant activities and total phe-nolics of different types of honey. Nutr. Res. 22 (2002) 1041-1047.

8. Gheldof, N., Engeseth, N.J.: Antioxidant capacity of honeys from various floral sour-ces based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J. Agric. Food Chem. 50 (2002) 3050-3055.

9. Wang, X.-H., Gheldof, N., Engeseth, N.J.: Effect of processing and storage on anti-oxidant capacity of honey. J. Food Sci. 69, 2 (2004) 96-101.

10. Mačukanović-Jocić, M.: The biology of melliferous plants with an atlas of Serbian apiflora. Belgrade, Serbia, Faculty of Agriculture (2010), p. 420.



302

11. Singleton, V. L, Orthofer, R., Lamuela-Raventos, R. M.: Methods in Enzymology, Oxidant and Antioxidants (Part A). In: Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Ed. Packer L. Academic Press, San Diego (1999) pp. 152-178.

12. Zhishen J., Mengcheng T., Jianming W.: The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64 (1999) 555-559.

13. Čanadanović-Brunet, J. M., Ćetković, G. S., Djilas, S. M., Tumbas, V. T., Savatović, S. S., Mandić, A. I., Markov, S. L. and Cvetković, D. D.: Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. Int. J. Food Sci. Techn. 44 (2009) 269-278.

14. Yen, G.C. and Chen H. Y.: Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43 (1995) 27-32.

15. Oyaizu M.: Studies on product of browning reaction prepared from glucose amine. Japan. J. Nutr. 44 (1986) 307-315.

16. Pravilnik o ocenjivanju kvaliteta pčelinjih proizvoda na Novosadskom sajmu, Novo-sadski sajam AD, Novi Sad, 2010 (in Serbian).

17. Savatović, S., Dimitrijević, D., Djilas, S., Čanadanović-Brunet, J., Ćetković, G., Tum-bas, V., Štajner, D.: Antioxidant activity of three different Serbian floral honeys. APTEFF 42 (2011) 145-155.

18. Amiot, M. J., Aubert, S., Gonnet, M., & Tacchini, M.: Les composés phénoliques des miels: étude préliminaire sur l'identification et la quantification par familles. Apidolo-gie 20 (1989) 115-125.

19. Meda, A., Lamien, C.E., Romito, M., Millogo, J., Nacoulma, O.G.: Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem. 91 (2005) 571-577.

20. Beretta, G., Granata, P., Ferrero, M., Orioli, M., Facino, R.M.: Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal. Chim. Acta 533 (2005) 185-191.

21. Bertoncelj, J., Doberšek, U., Jamnik, M., Golob, T.: Evaluation of the phenolic con-tent, antioxidant activity and colour of Slovenian honey. Food Chem. 105 (2007) 822-828.

22. Ramanauskiene, K., Stelmakiene, A., Briedis, V., Ivanauskas, L., Jakštas, V.: The quantitative analysis of biologically active compounds in Lithuanian honey. Food Chem. 132 (2012) 1544-1548.

23. Yao, L., Jiang, Y., Singanusong, R., Arcy, B.D., Datta, N., Caffin, N., Raymont, K.: Flavonoids in Australian Melaleuca, Guioa, Lophostemon, Banksia and Helianthus honeys and their potential for floral authentication. Food Res. Int. 37 (2004) 166-174.

24. Tomas-Barberan, F. A., Martos, I., Ferreres, F., Radovic, B. S., Anklam, E.: HPLC flavonoid profiles as markers for the botanical origin of European unifloral honeys. J. Sci. Food Agric. 81 (2001) 485-496.

25. Donovan, J. L., Meyer, A. S., Waterhouse, A. L.: Phenolic composition and antioxi-dant activity of prunes and Plum juice (Prunus domestica), J. Agric. Food Chem. 46 (1998) 1247-1252.



303

26. Fang, N., Yu, S., Prior, R. L.: LC/MS/MS characterization of phenolic constituents in dried plums, J. Agric. Food Chem. 50 (2002) 3579-3585.

27. Nuñez de Gonzalez, M.T., Boleman, R.M., Miller, R.K., Keeton, J.T., Rhee, K.S.: Antioxidant Properties of Dried Plum Ingredients in Raw and Precooked Pork Sau-sage. J. Food Sci. 73, 5 (2008) H63-H71.

28. Cimpoiu, C., Hosu, A., Miclaus, V., Puscas, A.: Determination of the floral origin of some Romanian honeys on the basis of physical and biochemical properties, Spectro-chimica Acta Part A, Spectrochim. Acta A: Mol. Biomol. Spectrosc. (2012) in press. http://dx.doi.org/10.1016/j.saa.2012.04.008

29. Zalibera, M., Staško, A., Šlebodová, A. Viera Jančovičová, Tatiana Čermáková, Vlas-ta Brezová: Antioxidant and radical-scavenging activities of Slovak honeys – An elec-tron paramagnetic resonance study. Food Chem. 110 (2008) 512-521.

30. Baltrusaityté, V., Venskutonis, P. R., Čeksteryté, V.: Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chem. 101 (2007) 502-514.

31. González-Miret, M. L., Terrab, A., Hernanz, D., Fernández-Recamales, M. A., Here-dia, F. J.: Multivariate correlation between color and mineral composition of honeys and by their botanical origin. J. Agric. Food Chem. 53 (2005) 2574-2580.

32. Frankel, S., Robinson, G. E., Berenbaum, M. R.: Antioxidant capacity and correlated characteristics of 14 unifloral honeys. J. Apicult. Res. 37 (1998) 27-31.

АНТИОКСИДАТИВНЕ И СЕНЗОРНЕ КАРАКТЕРИСТИКЕ БАГРЕМОВОГ

МЕДА СА ДОДАТКОМ СУВИХ ШЉИВА

Весна Т. Тумбаса*, Јелена Ј. Вулића, Јасна М. Чанадановић-Брунета, Соња М. Ђиласа, Гордана С. Ћетковића, Слађана С. Стајчића, Дубравка И. Штајнерб

и Борис М. Поповићб

а Универзитет у Новом Саду, Технолошки факултет Нови Сад, Булевар цара Лазара 1, 21000 Нови Сад,

Србија б Универзитет у Новом Саду, Пољопривредни факултет, Трг Доситеја Обрадовића 8, 21000 Нови Сад,

Србија

У багремов мед су додате суве шљиве у масеним концентрацијама 20, 30 и 40%. Утицај сувих шљива на особине меда испитан је мерењем садржаја укупних фенол-них једињења и флавоноида, као и антиоксидативне активности. Са повећањем концентрације сувих шљива у меду укупан садржај фенолних једињења повећао се 2,5 пута (од 16,18 до 41,64 mgGAE/100g), док је повећање садржаја флавоноида још веће, око 11,5 пута (од 2,65 до 30,86 mgRE/100g). Додатак сувих шљива утицао је и на повећање антиоксидативне активности меда. Узорци меда са 40% сувих шљива показали су највећу антиоксидативну активност, која је одређена на слободне хид-роксил радикале (EC50

•OH=4,56 mg/ml) и 2,2-дифенил-1-пикрилхидразил (DPPH) ра-дикале (EC50

DPPH=16,48 mg/ml), као и тестом редукционе способности (EC50RP=81,17

mg/ml). Судећи по утврђеним високим коефицијентима корелације, у опсегу од



304

0,771 до 0,947 за фенолна једињења, односно у опсегу од 0,862 до 0,993 за фла-воноиде, може се закључити да ова једињења учествују у механизмима антиокси-дативног деловања узорака меда. Са друге стране, сензорне карактеристике меда са додатком сувих шљива оцењене су нижом оценом од чистог багремовог меда, при чему је додатак шљива имао најмањи утицај на укус. Резултати испитивања су показали да се додатком сувих шљива у мед побољшава антиоксидативна актив-ност меда повећањем садржаја полифенолних једињења у њему, уз мале модифик-ације сензорних карактеристика. Кључне речи: багремов мед, суве шљиве, фенолна једињења и флавоноиди, анти-

оксидативна активност, сензорне карактеристике

Received: 03 September 2012 Accepted: 29 October 2012

antioxidant and sensorial properties of acacia …sensorial properties introduction honey is a...

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