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ANTIMICROBIAL FINISHING IN TEXTILES ASSIGNMENT

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Page 1: Antimicrobial Finish in Textiles

ANTIMICROBIAL FINISHING IN TEXTILES

ASSIGNMENT

Page 2: Antimicrobial Finish in Textiles

INTRODUCTION

The use of antimicrobials dates back to ancient Egypt, where these were used in the treatment of

mummies . The first antimicrobial textile material, in modern history, was developed by Lister in

1867. Over the last few years there has been increased interest in antimicrobial finishes. The

main reasons for this increased interest include: the promotion of healthier and physically active

lifestyle; an increased awareness of the harmful effects of organisms on textiles as well as on

human hygiene and freshness , and the greater use of synthetic fibres and blends in items such as

shirts, hosiery, blouses and underwear, which tend to cause greater ‘perspiration wetness’

because of poor moisture transport properties as compared to natural fibres.

Other factors that are responsible for the current level of interest include the increasing use of

carpeting and architectural fabrics . Consumers are becoming more conscious of carpet odours

created by bacteria, mold and mildew. Protection of fabrics, used in buildings, from fungi that

cause staining, odour and damage is critical in the outdoor environment.

Antimicrobial finish in textiles :

The consumers are now increasingly aware of the hygienic life style and there is a necessity and

expectation for a wide range of textile products finished with antimicrobial properties, says O L

Shanmugasundaram.

The inherent properties of the textile fibres provide room for the growth of micro organisms.

Besides, the structure of the substrates and the chemical processes may induce the growth of

microbes. Humid and warm environment still aggravate the problem. Infestation by microbes

cause cross infection by pathogens and development odour where the fabric is worn next to skin.

In addition, the staining and loss of the performance properties of textile substrates are the results

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of microbial attack. Basically, with a view to protect the wearer and the textile substrate itself

antimicrobial finish is applied to textile materials.

Historical Account:

During World War II, when cotton fabrics were used extensively for tentage, tarpaulins and truck

covers, these fabrics needed to be protected from rotting caused by microbial attack. This was

particularly a problem in the South Pacific campaigns, where much of the fighting took place

under jungle like conditions. During the early 1940s, the US army Quartermaster Crops collected

and compiled data on fungi, yeast and algae isolated from textiles in tropical and subtropical

areas throughout the world.

Cotton duck, webbing and other military fabrics were treated with mixtures of chlorinated

waxes, copper and antimony salts that stiffened the fabrics and gave them a peculiar odour. At

the time, potential polluting effects of the application of, these materials and toxicity-related

issue were not a major consideration. After World War II, and as late as the mid-to-late 1950's

fungicides used on cotton fabrics were compounds such as 8-hydroxygiunoline salts, copper

naphthenate, copper ammonium fluoride and chlorinated phenols. As the government and

industrial firms became more aware of the environmental and workplace hazards these

compounds caused. Alternative products were sought. A considerable amount of work was done

by the Southern Regional Research Laboratory of the US Department of Agriculture, the

Institute of Textile Technology (ITT) and some of the ITTs member mills to chemically modify

cotton to improve its resistance to rotting and improve other properties by acetylation and

cyanoethylation of cotton. These treatments had limited industry acceptance because of relatively

high cost and loss of fabric strength in processing. In addition, the growing use of man-made

fibres such as nylon, acrylics and polyester, which have inherent resistance to microbial

decomposition, came into wider use to replace cotton in many industrial fabrics.

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What are Microbes?

Microbes are the tiniest creatures not seen by the naked eye. They include a variety of micro

organisms like bacteria, fungi, algae and viruses. Bacteria are unicellular organisms, which grow

very rapidly under warmth and moisture. Further, sub divisions in the bacteria family are Gram

positive (Staphylococcus aureus), Gram negative (E-Coli), spore bearing or non-spore bearing

type.

Some specific types of bacteria are pathogenic and cause cross infection. Fungi, molds or mildew

are complex organisms with slow growth rate. They stain the fabric and deteriorate the

performance properties of the fabrics. Fungi are active at a pH level of 6.5. Algae are typical

micro organisms, which are either fungal or bacterial. Algae require continuous sources of water

and sunlight to grow and develop darker stains on the fabrics. Algae are active in the PH range of

7.0-8.0. Dust mites are eight legged creatures and occupy the household textiles such as blankets

bed linen, pillows, mattresses and carpets. The dust mites feed on human skin cells and liberated

waste products can cause allergic reactions and respiratory disorders.

Some harmful species of the bacteria and fungi are listed in Table 1.

Bacteria FungiGram positive bacteria Cloth damaging fungi

Staphylococcus aurues or Pyogens Aspergillus nigerStaphylococcus epidermidis Aspergillus furnigatus

Corynebacterium Curvularia lunotaGram negative bacteria Crop damaging fungi

Escherichia coli Fusarium speciesProteus vulgaris Sclerotium rolfsii

Effects of Microbes on Textiles and Humans

Although microbes can be useful in many ways, for example in brewing, baking and

biotechnology, they can also be harmful to both textiles and humans. Different substances added

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to textiles, such as size, hand modifiers, antistats, thickeners, lubricants and dirt as well as

grease, sweat and dead skin from the human body provide a great source of nourishment for

microorganisms. Following are some of the possible effects of micro-organisms on textiles:

1. Bad odour

2. Discoloration, unsightliness or staining of the fabric

3. A slick slimy handle

4. Loss of functional properties like elasticity and tensile strength

5. Decrease in the life of the textiles, especially cotton and wool

Natural fibres are particularly prone to microbial attack as they are readily digestible by bacteria

and fungi but the synthetic fibres are not completely immune either as they might contain some

additives that may be a nutrient for microbes. Wool is more likely to be attacked by bacteria than

cotton while cotton is more likely to be attacked than wool by fungi. Many different finishes

applied on textiles are excellent source of nutrients for microbes, making textiles susceptible to

microbial attack. Work in progress in textile manufacturing is also susceptible to microbial

attack, particularly if the goods are left for a long time in conditions conducive for microbial

growth.

Apart from their effects on textiles, microbes can be a source of many problems to human

beings, ranging from simple unpleasant odour to physical irritation, allergy, toxic responses,

infection and diseases. An unpleasant odour results when, among other things, human

perspiration is converted by bacteria into foul-smelling substances such as aldehydes, amines,

ammonia, sulphides, mercaptans, aromatics, lactones and fatty acids (acetic, propionic, butyric,

valerianic, caproic).

The minute particles of house dust mite excrements adhering to dust particles can cause dust

allergies while mould fungi of the aspergillus type can produce lung disease.

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Necessity of antimicrobial finishes :

Antimicrobial treatment for textile materials is necessary to fulfill the following objectives:

1. To avoid cross infection by pathogenic micro organisms.

2. To control the infestation by microbes.

3. To arrest metabolism in microbes in order to reduce the formation odour.

Requirements for antimicrobial finish :

Textile materials, in particular the garments are more susceptible to wear and tear. It is important

to take into account the impact of stress strain, thermal and mechanical effects on the finished

substrates. The following requirements need to be satisfied to obtain maximum benefits out of

the finish:

1. Durability to washing, dry-cleaning and hot pressing.

2. Selective activity to undesirable micro organisms.

3. Should not produce harmful effects to the manufacturer, user and the environment.

4. Should comply with the statutory requirements of regulating agencies.

5. Compatibility with the chemical processes.

6. Easy method of application. No deterioration of fabric quality.

7. Resistant to body fluids; and resistant to disinfections/sterilisation.

Antimicrobial finishing methodologies :

The antimicrobial agents can be applied to the textile substrates by exhaust, pad-dry-cure,

coating, spray and foam techniques. The substances can also be applied by directly adding into

the fibre spinning dope. It is claimed that the commercial agents can be applied online during the

dyeing and finishing operations. Various methods for improving the durability of the finish

include:

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1. Insolubilisation of the active substances in/on the fibre.

2. Treating the fibre with resin, condensates or cross-linking agents.

3. Micro encapsulation of the antimicrobial agents with the fibre matrix.

4. Coating the fibre surface.

5. Chemical modification of the fibre by covalent bond formation.

6. Use of graft polymers, homo polymers and/or co-polymerisation on to the fibre.

Mechanism of antimicrobial finishes :

Normal home-washing of textiles, which is generally under mild conditions, does not completely

remove the microbes. In order to eliminate microbes, very severe laundering conditions, e.g., a

temperature of 95 °C and strong detergents followed by bleach, are essential. Any surviving

microbes can quickly multiply again at each further wearing. This can be avoided by the

application of antimicrobial finishes.

Antimicrobial is an agent that works against microbes. It can either inhibit their growth and

b.reduce the undesired by-products or kill them altogether. Agents which inhibit the growth of

microorganisms and do not kill them are known as biostats, i.e. bacteriostats, fungistats. Agents

which actually kill the microorganisms are known as biocides, i.e. bacteriocides, fungicides.

While biostats are subject to fewer regulations, biocides are more strictly controlled by

governmental regulations.

All antimicrobials do not work in the same manner. They primarily function in two different

ways. The conventional leaching types of antimicrobials work by leaching or moving from the

surface on which they are applied. They leave the textile and chemically enter or react with the

microorganism acting as a poison. Like an arrow shot from a bow or a bullet shot from a gun,

they are used up in the process of working, wasted in random misses, or complexed with other

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chemicals in the environment for use or abuse. Leaching types of antimicrobials can be effective

against microbes on the fibre surface or in the surrounding environment. However, they may

have the following disadvantages:

1. Poor durability, as the antimicrobial reservoir will be eventually depleted

2. Can contact the skin and potentially affect the normal skin bacteria

3. Can cross the skin barrier and have the potential to cause rashes and other skin irritations

4. Allow for the adaptation of microorganisms

The second type of antimicrobials consists of molecules that are chemically bound to fibre

surfaces. The bound antimicrobials remain affixed to the substrate, killing microbes as they

contact the surface to which antimicrobials have been applied. They control only those microbes

that are present on the fibre surface and are not effective in the surrounding environment. They

can potentially be abraded away from the fibre surface or may become deactivated and lose long

term durability.

The actual mechanism of control of microorganisms by antimicrobials is extremely varied,

ranging from poisoning the cell from within, preventing cell reproduction, blocking of enzymes,

and reaction with cell membrane to the destruction of cell walls.

Textiles Normally Subjected to Antimicrobial Finishes

Apparel/Sportswear/leisurewear/workwear: to get rid of the bad odour produced by

the bacterial decomposition of sweat and other body fluids.

Undergarments (including underwear worn by astronauts): to control the growth of

bacteria which thrive on suitable body temperature and moisture conditions, leading to

skin diseases.

Socks: to make them free from unpleasant odour by controlling the growth of microbes.

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Shoes: to avoid shoe odour and the stains on the shoelaces caused by bacterial growth,

especially in rainy season.

Home Furnishings and Upholstery: to inhibit the growth of bacteria leading to

degradation and/or staining in carpets, shower curtains, mattress ticking, pillow covers,

pillow fills and upholstery.

Industrial Fabrics Exposed to Weather: to protect fabrics used for awnings, tents,

tarpaulins and the like from rotting and mildew.

Sized Fabrics: to protect microbial growth due to the presence of warp size.

Wet Textiles: to prevent degradation of fabrics by microbial attack during prolong

storage between processing steps.

Medical Textiles: to impart antimicrobial properties on wound dressings and get

freedom from microbes/pathogens from textiles used in hospitals, nursing homes,

medical and research facilities, and veterinary clinics for protection against infectious

diseases.

Textiles in Museums: to preserve textile monuments from microbial attack.

Antimicrobial substances and their effect:

Many antimicrobial agents used in the textile industry are known from the food stuff and

cosmetics sector. These substances are incorporated with textile substrates comparatively at

lower concentrations. It must be ensured that these substances are not only permanently effective

but also that they are compatible with skin and the environment. A wide palette of antimicrobial

compounds is now in use but differ in their mode of action. The following list demonstrates the

polyvalent effect of the various antimicrobial substances:

1. Materials with active finishes contain specific active antimicrobial substances, which act

upon micro organisms either on the cell, during the metabolism or within the core

substance (genome). However, due to the very specific nature of their effect, it is

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important to make a clear distinction between antibiotics and other active substances,

which have abroad range of uses.

2. Oxidising agents such as aldehydes, halogens and proxy compounds attack the cell

membrane, get into the cytoplasm and affect the enzymes of the micro organisms.

3. Coagulants, primarily alcohols irreversibly denature the protein structures. Radical

formers like halogens, isothiazones and peroxo compounds are highly reactive due to the

presence of free electrons. These compounds virtually react with all organic structures in

particular oxidising thiols in amino acids. Even at the lowest level of concentrations,

these substances pose particular risk to nucleic acids by triggering mutations and

dimerisation.

4. One of the most durable types of antimicrobial products is based on diphenyl ether (bis-

phenyl) derivative known as either 2, 4, 4'-trichloro-2' hydroxy dipenyl ether or 5-chloro-

2-(2, 4-dichloro phenoxyl) phenol. Triclosan products have been used for more than 25

years in hospitals and personal care products such as antimicrobial soap, toothpaste and

deodorants. Triclosan inhibits growth of micro organisms by using an electro-chemical

mode of action to penetrate and disrupt their cell walls. When the cell walls are

penetrated, leakage of metabolites occurs and other cell functions are disabled, thereby

preventing the organism from functioning or reproducing. The Triclosan when

incorporated within a polymer migrates to the surface, where it is bound. Because, it is

not water-soluble, it does not leach out, and it continuously inhibits the growth of bacteria

in contact with the surface using barrier or blocking action.

5. Quaternary ammonium compounds, biguanides, amines and glucoprotamine show poly

cationic, porous and absorbent properties. Fibres finished with these substances bind

micro organisms to their cell membrane and disrupt the lipo polysaccharide structure

resulting in the breakdown of the cell.

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6. Complexing metallic compounds based on metals like cadmium, silver, copper and

mercury cause inhibition of the active enzyme centers (inhibition of metabolism).

Amongst these, the silver compounds are very popular and already been used in the

preparation of antimicrobial drinking water.

7. Chitosan is an effective natural antimicrobial agent derived from Chitin, a major

component in crustacean shells. Coatings of Chitosan on conventional fibres appear to be

the more realistic prospect since; they do not provoke an immunological response. Fibres

made from Chitosan are also available in the market place. Natural herbal products can

be used for antimicrobial finishes since, there is a tremendous source of medicinal plants

with antimicrobial composition to be the effective candidates in bringing out herbal

textiles.

Commercial antimicrobial agents and fibres :

Thomsan Research Associates markets a range of antimicrobials under the trade name Ultrafresh

for the textile and polymer industry. Ultra fresh products were developed to be used in normal

textile processes. Most ultra fresh treatments are non-ionic and are compatible with a wide range

of binders and finishes. To incorporate antibacterial into high temperature fibres like polyester

and nylon, it is necessary to use an inorganic antimicrobial like Ultrafresh CA-16 or PA-42.

These must be added as a special master batch to the polymer mixture before the extrusion

process.

For fibres such as polypropylene, which are extruded at lower temperatures, it is possible to use

organic antimicrobials such as Ultra fresh Nm-100, Dm-50 or XQ-32. In the case of Rossari.s

Fabshield with AEGIS microbe shield programme, the cell membrane of the bacteria get

ruptured when the microbes come in contact with the treated surface; thus, preventing

consumption of antimicrobial over a period of time and remain functional throughout the life of

the product. The active substance 3-Trimethoxy silyl propyl dimethyl octadecyl ammonium

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chloride gets attached to the substrate either through bond formation on the surface or by

micropolymer-sing and forming a layer on the treated surface; the antimicrobial agent disrupts

the cell membrane of the microbes through physical and ionic phenomena.

Ciba Specialty Chemicals markets Tinosan AM 110 as a durable antimicrobial agent for textiles

made of polyester and polyamide fibres and their blends with cotton, wool or other fibres.

Tinosan contains an active antimicrobial (2, 4, 4'-Trichloro-2' - hydroxyl-dipenylether) which

behaves like a colourless disperse dye and can be exhausted at a very high exhaustion rate on to

polyester and polyamide fibres when added to the dye bath. Clariant markets the Sanitised range

of Sanitized AG, Switzerland for the hygienic finish of both natural and synthetic fibres. The

branded Sanitised range function as a highly effective bacteriostatic and fungistatic finishes and

can be applied to textile materials such as ladies hosiery and tights.

ANTIMICROBIAL TEXTILES

Actigard finishes from Clariant are used in carpets to combat action of bacteria, house dust mites

and mould fungi. Avecia.s Purista-branded products treated with Reputex 20 which is based on

poly (hexamethylene) biguanide hydrochloride (PHMB) claimed to posses a low mammalian

toxicity and broad spectrum of antimicrobial activity. PHMB is particularly suitable for cotton

and cellulosic textiles and can be applied to blends of cotton with polyester and nylon. In

addition to the aforesaid antimicrobial agents, the fibres derived from synthetic with built-in

antimicrobial properties are listed in Table 2.

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Benefits of antimicrobial textiles

A wide range textile product is now available for the benefit of the consumer. Initially, the

primary objective of the finish was to protect textiles from being affected by microbes

particularly fungi. Uniforms, tents, defence textiles and technical textiles, such as, geo-textiles

have therefore all been finished using antimicrobial agents. Later, the home textiles, such as,

curtains coverings, and bath mats came with antimicrobial finish. The application of the finish is

now extended to textiles used for outdoor, healthcare sector, sports and leisure.

Novel technologies in antimicrobial finishing are successfully employed in nonwoven sector

especially in medical textiles. Textile fibres with built-in antimicrobial properties will also serve

the purpose alone or in blends with other fibres. Bioactive fibre is a modified form of the finish,

which includes chemotherapeutics in their structure, ie, synthetic drugs of bactericidal and

fungicidal qualities. These fibres are not only used in medicine and health prophylaxis

applications but also for manufacturing textile products of daily use and technical textiles.

The field of application of the bioactive fibres includes sanitary materials, dressing materials,

surgical threads, materials for filtration of gases and liquids, air conditioning and ventilation,

constructional materials, special materials for food industry, pharmaceutical industry, footwear

industry, clothing industry, automotive industry, etc.

Antimicrobial finishes add value to the product for both the producer and the consumer in

the following ways:

1. Protection of the raw material from decay or degradation

2. Control of staining caused by microbial growth

3. Added freshness to the fabrics

4. Elimination of smells created by microorganisms

5. Increased life period of the fabric because of control of the growth of microbes

6. Improvement in the handle for most of the fabrics

7. No irritation of the skin and no physiologic impact to human health

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Requirements for Effective Antimicrobial Finishes

Following are the major requirements for an effective antimicrobial finishing agent:

Quick acting and effective in killing or inhibiting the growth of a broad spectrum of

microorganisms

Non-selective and non-mutable to pathogens

Fast to repeated laundering, dry-cleaning and exposure to light

Safe and easy to apply

Safe and comfortable to wear (no irritation of the skin/dermatologically tested)

Minimal environmental impact

Compatible with other finishing agents

Low cost

There is no such thing as universal antimicrobial. The choice of a suitable antimicrobial depends

on whether it is meant to combat odour or staining, or inhibit the growth of fungi, mildew or

bacteria. Furthermore, the different ‘anatomy’ of the microorganism also plays a role in the

selection of a suitable antimicrobial.

Chemistry of Antimicrobial Finishes for Textiles

Antimicrobial chemicals are mainly of two types: those which work by controlled release

mechanism and those which remain bound to the textile substrate. Examples of antimicrobials

for controlled release are: Copper naphthenate, copper-8-quinolinate and various organo mercury

compounds; tributyl tin oxide, dichlorophene and 3-iodopropynylbutyl carbamate; Benzimidazol

derivatives, salicylanilides and alkylolamide salts of undecylenic acid; Formaldehyde; Triclosan

(2,4,4’-trichloro-2’-hydroxydiphenyl ether); Quaternary ammonium salts; Organo-silver

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compounds and silver zeolites. Examples of bound antimicrobials are:

Octadecylaminodimethyltri-methoxysilylpropylammonium chloride; Polyhexamethylene

biguanide (PHMB) and Methylol-5,5-dimethyldyantoin + hypochlorite.

Chitosan, manufactured from inexpensive natural waste product (crustacean shells such as crab

etc.), has been used with great success in Japanese domestic market for surgical dressings

because of its skin-healing properties and also has undergone successful trials in EU and UK

markets. It can be applied by microencapsulation or by reactive bonding to cellulose and by

crosslinking. When applied to cellulose by crosslinking, it gives antimicrobial as well as

moisture control properties. It offers many advantages including high absorbency properties,

moisture control, promotion of wound healing, non-allergenic, non-toxic and biodegradable

properties.

Application of Antimicrobial Finishes on Textiles

Technologies that are utilized to help keep the active antimicrobial ingredients on textile

materials wash after wash can be classified into four categories: surface application, chemical

bonding, internal antimicrobial release, and micro-encapsulation.

Surface application:

This method has the following characteristics:

It is applicable to all fibres

Washing durability depends normally on the affinity of antimicrobials

In the case that polymeric coating products are used together, washing durability depends

on the strength with which the polymers can bind with the textile surface

For certain fibres, such as PAN, ionic charge could be another factor to be considered

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Chemical bonding

This method has the following characteristics:

It is applicable only to those fibres that have a suitable reactive group, e.g. cellulose,

wool and polyamide.

Washing durability depends normally on the strength of the chemical bond between the

antimicrobial agent and the fibre.

It is theoretically the best way to achieve durable antimicrobial finish.

Internal antimicrobial release

This method has the following characteristics:

Antimicrobials are incorporated into the fibres when they are spun.

It is a viable option only for synthetic fibres.

It does not work well on cotton.

Micro-encapsulation

This method has the following characteristics:

It is a hybrid of “chemical bonding and controlled internal release”.

In this method, instead of antimicrobials making the chemical bond with fibres, micro-

capsules that contain the antimicrobials are covalently fixed on the fibres.

Effective antimicrobial effect depends on the particular capsule system to regulate the

release of antimicrobials in a sustained way.

Problems with Antimicrobial Finishes

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Some common problems related with the application of antimicrobial finishes are given as

follows:

1. Stiff Hand and Fabric Strength Loss: this may be caused by the use of binders and

resins with controlled-release finishes.

2. Colour Change: this may be due to inappropriate choice of antimicrobial finishes.

3. Selectivity: Some antimicrobial finishes are efficient against Gram-positive bacteria or

Gram-negative bacterial, while others against fungi. A formulation that is a mixture of

several substances will be more effective for good all-round protection against microbes.

4. Toxicological and environmental problem: include skin irritation, sensitizing, allergy

or dermatitis on prolonged contact with skin, biodegradability and bioaccumulation.

5. Handling: the higher the biocide activity, the higher the need for safe handling and

prevention from toxicity.

6. Development of resistant microbes: an area of concern regarding the use of

antibacterial finishes is that their long term use may lead to the development of resistant

microbes which might have deadly consequences for humans.

Considerations for Antimicrobial Textile Product Development

Antimicrobial finishing should not be considered as a simple add-on finishing process. Some of

the important points that must be considered for the development of a textile product with

antimicrobial properties are given as follows :

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1. Identification and definition of the customer and the customer’s needs

2. Selection of the most appropriate fabric

3. Selection of the most appropriate antimicrobial on the basis of safety considerations,

regulatory compliance, application technology and efficacy

4. Selection of the most appropriate application technology

5. Setting up of a manageable testing and quality control program

Useful Terms and Definitions

Activity, n.: (of an antimicrobial agent) a measure of effectiveness of an antimicrobial

agent.

Antibacterial agent, n.: any chemical which kills bacteria (bactericide) or interferes with

the multiplication, growth or activity of bacteria (bacteriostat).

Antifungal agent, n.: any chemical which kills or inhibits the growth of fungi.

Antimicrobial agent, n.: any chemical which kills or inhibits the growth of

microorganisms.

Bacterial resistance, n.: (in textiles) resistance to the development of visible bacterial

growth and accompanying odours, resulting from bacterial degradation of fibres or soil

on them, as distinguished from musty fungal odours.

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Mildew resistance, n.: (in textiles) resistance to the development of unsightly fungal

growths and accompanying unpleasant, musty odours on carpet materials exposed to

conditions favouring such growth.

Rot resistance, n.: (in textiles) resistance to deterioration of carpet material as a result of

fungal growth in or on it.

Zone of inhibition, n.: clear area of no growth of a microorganism, cultured onto the

surface of agar growth medium, in proximity to the borders of a specimen placed in direct

contact with this agar surface.

Evaluation of the influence of module and fungi

The influence of mould fungi is evaluated by three practical test methods:

1. At the growth test with a mixture of five different mould fungi it is evaluated how far the

textile is supporting the fungus growth. The evaluation is not done only visually, but also

material specific force elongation ratio is measured.

2. In an inhibition zone test, the question is answered, if the tested finishing agent is

protecting the textile from mould stains and mould over growth. The evaluation is done

by rating the fungus growth in contact to test material and the viewing of the inhibition

zone around the test sample in consequence of the diffusion of the antifungal agent.

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3. The third test the so called wet chamber test answers the question how a mould fungus

contaminated textile performs in the wet chamber the evaluation is done visually by

viewing the degree of growth or through tensile strength test.

Conclusion

With advent of new technologies, the growing needs of the consumer in the wake of health and

hygiene can be fulfilled without compromising the issues related to safety, human health and

environment. Taping new potential antimicrobial substances, such as, Chitosan from nature can

considerably minimise the undesirable activities of the antimicrobial products.

Scientists all over the globe are working in the area and a few of them reported to have used

antimicrobial finishes and fluoro chemicals to make the fabric having antimicrobial as well as

blood repellant properties. Chitosan and fluoro polymers are reported to be most suitable

finishing agents for medical wears with barriers against micro organisms and blood. To carve a

niche for textile materials, this kind of value adding finishes are the need of the hour.

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