annotated spectra for table ii ps: phosphoserine pt: phosphothreonine
DESCRIPTION
Annotated spectra for table II pS: phosphoserine pT: phosphothreonine p(SS) phosphorylation site localised to the two serines in brackets p(XXX) phosphorylation site localised to the residues in brackets * denotes oxidation # denotes deamidation. AVpSDIKHEVQSQLLQNEK Triply charged - PowerPoint PPT PresentationTRANSCRIPT
Annotated spectra for table II
pS: phosphoserinepT: phosphothreoninep(SS) phosphorylation site localised to the two serines in bracketsp(XXX) phosphorylation site localised to the residues in brackets* denotes oxidation# denotes deamidation
AVpSDIKHEVQSQLLQNEKTriply chargedProtein:At2g01680Score:62
051114_sm2013_56 #3134 RT: 35.21 AV: 1 NL: 2.31E5T: ITMS + c NSI d w Full ms2 [email protected] [ 185.00-2000.00]
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
683.6483
631.2993
959.4132744.4744
545.5248
931.0585830.0607
1096.5028481.2786
390.2550
316.25791186.2246
245.0992 1281.9001 1391.5133 1596.6573 1730.6359
b3-98y5
2+
b5-98
b82+
y3
[b10-98]2+
y5
[M+3H+-98]3+
y8y6
[b15-98]2+
y10
y9
YQp(SHS)FDDGEINPFANPTSVPAATSKProtein:At2g20840Score:52
060619_sm3001_41 #2889 RT: 46.60 AV: 1 NL: 4.99E2T: ITMS + c NSI d w Full ms2 [email protected] [ 250.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
574.3379
694.5594
1072.4230
895.4314
1473.4564
1359.3922
751.3501
430.2075 1246.4811
958.4524479.8795
1788.9009
1903.7140381.2576 1527.7191 1658.5365
1936.8412
y14
y10
y6
y102+
y142+
y152+
[M+2H+-98]2+
y11 y12
y14
b11
b10
b15
b16
MNSGSMApSPPVPEGGAGTGVVRProtein:At2g28070Score:97
060619_sm3001_05 #2117 RT: 40.64 AV: 1 NL: 1.72E4T: ITMS + c NSI d w Full ms2 [email protected] [ 280.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
999.5374
1292.7090
972.5265
773.46721139.3287
748.25291459.6622
1195.6908908.5154588.2815 1531.72201866.72021365.4768531.3669 1661.7997430.3354
y16
y15
y14
y13
y11
[M+2H+-98]2+
b12
b21-98y17
y9
b8-98
y6
y5y4
GAp(TS)GPLDPPAGEISRProtein:At2g35350Score:72
051008_sm1096_10 #2976 RT: 42.25 AV: 1 NL: 2.20E5T: ITMS + c NSI d w Full ms2 [email protected] [ 210.00-1620.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
826.5321663.3594
745.0962
941.4194548.3125
1151.54111044.3931375.4149 452.2943 1343.5231262.2230 857.4508 1456.8458
b7-98
y3y2
y5
b7 y8
[M+2H+-98]2+
y10
y9
TMPSMpSPSSVSTEKProtein:At2g36330Score:57
060619_sm3001_11 #1986 RT: 39.30 AV: 1 NL: 5.77E3T: ITMS + c NSI d w Full ms2 [email protected] [ 200.00-1560.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
659.0012
725.9451
834.3873
1316.51101034.3794
1001.3088463.2272
495.3430385.1769 903.46531244.79641132.5262292.2166 1420.7203
y4
y112+
y6
y122+
y8
[M+2H+-98]2+
y9-98y9
y10-98
y10
y12
TSEPpTPASGTSTITQRProtein:At2g38480Score:78
051114_sm2013_29 #1713 RT: 26.25 AV: 1 NL: 9.77E3T: ITMS + c NSI d w Full ms2 [email protected] [ 225.00-1725.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
699.0162
649.9877 808.3412
1118.5811
950.5161
404.3408
498.1473517.4042 1292.3635 1396.4559863.4966 1021.4980279.1914 1521.4886
y3
y11
y9
y132+
[y13-98]2+
y10y8y13
y13-98
SSDLYGLSLpSSRProtein:At2g39660Score:73
060718_sm3007_33 #6084 RT: 69.65 AV: 1 NL: 1.40E3T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-1380.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
799.2805
629.3328
962.3173
736.1974
566.1932
718.3081
429.1410531.4181331.3426
701.4973918.1875444.2563
823.3660290.1288 403.2125
864.6121262.23761092.4807
1190.3333237.4301
y4-98
y3-98y3
y2
b5
y5-98
y5
[M+2H+-98]2+
b7
y7-98
y7
y8
b9
p(SS)DLYGLSLSSRProtein:At2g39660Score:68
051114_sm2013_11 #3172 RT: 36.30 AV: 1 NL: 2.19E3T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-1380.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
633.8369
719.4729
549.3590
882.4878
349.2318
462.4709
815.98171016.4117483.0226 900.4338255.0922
1103.2015210.6552
y5
y4
y3
[M+2H+-98]2+
b9
y8
y7
p(TSFT)LEEDPHASPLSPKProtein:At3g17650Score:50
051010_sm1096_33 #3050 RT: 44.15 AV: 1 NL: 3.17E4T: ITMS + c NSI d w Full ms2 [email protected] [ 255.00-1950.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
Re
lative
Ab
un
da
nce
920.18
1003.28
887.35
1177.471048.53
772.24628.44
541.51
834.69 1306.57643.32
1595.541141.53
1210.42699.43514.26 1419.521279.30
331.31 1507.35496.411395.48
1674.52413.40
393.351870.441803.76
1897.85
b15-98
y13
y5
y3
y6
y8
y7
b7-98
y11y10
b8
y12
NYDETGpSQLQASPSVVSRProtein:At3g22180Score:80
060718_sm3007_13 #2291 RT: 35.33 AV: 1 NL: 1.87E4T: ITMS + c NSI d w Full ms2 [email protected] [ 265.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
960.5638644.4410
811.3420882.3600
1123.4652
1276.3877784.4607
1207.3320361.2935
1395.5499
1496.55111639.2726713.4091
1559.5038 1756.4015
1025.5406
460.3500547.5125
1825.5253
y6
y5
y4
y3
y8-98
y8
y7-98
y7
[M+2H+-98]2+
y10-98
y10
b11
y14
y13
b12-98
b15-98b16
AVWADMGPAMpSDHGDGKProtein:At3g26935Score:86
060619_sm3001_37 #3369 RT: 43.87 AV: 1 NL: 1.14E5T: ITMS + c NSI d w Full ms2 [email protected] [ 240.00-1840.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
864.1112
996.42831053.4022
1151.3245 1282.4865
1184.4811778.7562
543.2580 731.1179
373.2037 674.3067 1621.4803513.3375
1523.4851628.2536428.1774
958.7233 1370.5323
1468.4519
1675.5679
b7
y7
b6
b5
y5
b4y6
[M+2H+-98]2+
y12-98
y11-98
y11
y10-98
y10
y12
b15
b15-98
NAHPPEPETLDGNM*DAGAGQpTPQLRTriply chargedProtein:At3g26935Score:90
051010_sm1096_45 #2614 RT: 39.34 AV: 1 NL: 2.25E4T: ITMS + c NSI d w Full ms2 [email protected] [ 235.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
513.4319
1007.4139
872.6711781.4689
1052.0490
950.4496
694.32011269.6345
1193.4094596.3979
1372.5562416.3462324.1938 1705.5085
1519.4736 1890.6724
y5-98
y5
y4
y3
y7-98
b19b16b14
b13
y9
[b21-98]2+
y11
b242+
KDEEPTPFpSGPVPRProtein:At3g27390Score:58
051010_sm1096_52 #2945 RT: 41.41 AV: 1 NL: 6.45E4T: ITMS + c NSI d w Full ms2 [email protected] [ 215.00-1650.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
769.5615
1036.5593
838.7152
525.4799
936.35471134.5867
700.4758502.3932
1369.17221165.5488682.3107355.2473
448.3433
1437.4619 1637.9629
y6-98y2
y5
b4
[M+2H+-98]2+
y10-98
b9-98
y8
y7
y11-98
y10
SSpSANSVTGPSTIKProtein:At3g28345Score:38
051114_sm2013_18 #1950 RT: 28.04 AV: 1 NL: 1.15E4T: ITMS + c NSI d w Full ms2 [email protected] [ 185.00-1430.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
650.6076
572.4866
889.4022
545.2811802.3997
1003.4163
1074.5601
730.3480527.0211
315.1927255.1508 423.2726 853.40641171.5374
1251.4778986.51311338.6211
Assignment of S3 based solely on [y12-98] 2+
Otherwise phosphorylation site can only be isolatedto either S1, S2 or S3
y12-98
b4-98
y5
y6
[M+2H+-98]2+
y11
y10
y9
y8
b13-98
SFpSFDVGPN#GGK# denotes deamidatedProtein:At3g28850Score:72
051008_sm1096_10 #3690 RT: 47.56 AV: 1 NL: 1.30E4T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1305.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
597.8915530.3112
665.3511
480.8105
744.41151058.4148
891.4489763.1699376.2939
960.4124722.3157235.1033451.1212
332.1455
402.2086820.2908 991.4429
1089.3301 1164.2943 1250.4125
y4
b3-98
y3
b2
y5
b4-98
y6
[M+2H+-98]2+
y10-98
y10
y9
y8
b5
ADFASAAIApTPPISKProtein:At1g01540Score:70
051010_sm1096_22 #3015 RT: 44.79 AV: 1 NL: 4.40E3T: ITMS + c NSI d w Full ms2 [email protected] [ 200.00-1555.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
541.4456
901.2242
722.3353
793.3325
818.3571
999.1973977.3928
634.2222
616.1967523.4048 1306.3430413.3773 1135.5256
1393.39091206.4967234.2880 377.34761477.5972
y9
y7
y6
y5
[b10-98]2+
y11
y10
b14b13
b9
y8
SGPLVAQpSPDSVIVKProtein:At1g18390 Score:69
051010_sm1096_13 #3371 RT: 44.62 AV: 1 NL: 3.08E4T: ITMS + c NSI d w Full ms2 [email protected] [ 205.00-1590.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1123.5123
740.1537
757.3994
924.3990
1025.4894454.2712
716.88441222.4625681.3773
436.2498 525.3045337.1746 1430.6295
906.3712 1332.5780954.5465632.2800
246.2446
y11
y10
y8
y7
y5
y10-98
b14b13
060718_sm3007_31 #4120 RT: 59.86 AV: 1 NL: 4.59E3T: ITMS + c NSI d w Full ms2 [email protected] [ 275.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1001.8042
1556.5701
910.21611102.4750
829.0289
446.2427 1499.65301231.5511503.2547
750.6333 1828.4880560.2202 1655.6222 1926.51121185.4287 1299.3939345.2589681.3394
y13
y11
y17
y202+
y10
y4
b19-982+
b19-98
pSGGYVGGPNEGGGGGGGWATAVR Protein:At1g30440 Score:123
y16
y14
[M+2H+-98]2+
Y18
Y19
p(SAS)FKEESDFFADLK Protein:At1g30690Score:72
051010_sm1096_42 #4134 RT: 50.07 AV: 1 NL: 8.71E4T: ITMS + c NSI d w Full ms2 [email protected] [ 235.00-1815.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
852.1208
773.1017
738.7195
1200.5138
1443.4978
1541.35851328.56421071.4844942.4374
260.2727375.2342 593.3627
1654.45131793.8342
[M+2H+-98]2+
y10
y2
b13-98
b13y9
b9
y8
y122+
y11
TLpSDPFSNGK Protein:At1g33990Score:56
051114_sm2013_26 #2730 RT: 33.13 AV: 1 NL: 3.73E3T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1160.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
524.3513 931.2563
649.3110
833.4088215.0392 417.2647
187.1904496.9889 537.0549
764.4056
730.3191
914.1727474.3753 712.4993325.1845 632.2982261.0578 899.0359 981.5924 1055.4941
[M+2H+-98]2+
y8
y7
y6
y8-98b2
y2
b4
TDSEVTSLAASSPARpSPR (triply charged) Protein:At1g45688 Score:60
051010_sm1096_43 #2530 RT: 38.09 AV: 1 NL: 4.56E3T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1925.00]
200 400 600 800 1000 1200 1400 1600 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
848.7085
690.9358
740.6575
560.1964
272.1995 415.2499
905.9037 1032.4048 1326.68881208.3497 1424.5144
y2
[y17-(98+18)]2+
y132+
y142+
[b4-18]
y3
y6
NIEDIFp(SSGS)RR (doubly phosphorylated)Protein:At1g59870Score:51
051010_sm1096_31 #3639 RT: 47.63 AV: 1 NL: 1.98E6T: ITMS + c NSI d w Full ms2 [email protected] [ 200.00-1555.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
722.0490
809.2621599.3581 956.2649437.4804
331.2461 1014.3059 1184.8262 1313.4037357.3824313.4263
y5
y112+
[M-98-98]2+
b4
[y8-196]2+
y6
y7
LpSPLPPEPVGFDYGR Protein:At1g61250 Score:73
060718_sm3007_13 #3603 RT: 43.13 AV: 1 NL: 4.98E4T: ITMS + c NSI d w Full ms2 [email protected] [ 225.00-1735.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
813.6040617.5012 1233.5260
910.4618
722.5609
491.2175
393.2087
1136.5266365.3130
1329.3396510.4414280.1413 1039.4210 1540.7603
1443.6217 1620.4753
y11
y8
y7
y132+
y112+
y10
y12b12
b4
b3-98y3
b4-98
p(SS)PVNSGAFVSK Protein:At1g62320 Score:45
060718_sm3007_11 #1967 RT: 33.91 AV: 1 NL: 1.19E4T: ITMS + c NSI d w Full ms2 [email protected] [ 160.00-1275.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
581.4633
503.3770 809.3234345.0449 400.2176244.1358 695.4353
1005.4438480.3348 910.3517664.3871286.1221 1026.2275893.2244 928.4543 1102.3422792.4969
[M+2H+-98]2+
y102+
[b4-98]2+
y10y9
y8y7y3
TVpSDVTIGRProtein:At1g66480Score:69
051114_sm2013_29 #1970 RT: 28.05 AV: 1 NL: 3.21E4T: ITMS + c NSI d w Full ms2 [email protected] [ 130.00-1040.00]
200 300 400 500 600 700 800 900 1000m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
465.4693 827.3296
446.3328
729.4436
232.2684484.2137173.0778
545.3901345.3061
660.4077
582.0706778.1522365.2217
698.2834414.7710 839.3347270.2207 647.3022
926.2787
y6
y5
y4
y3
y2
b2 [y7-98]2+
[y8-98]2+
b5
FHYQSGGSGGGGGAHYPAPNPGMFpSPNTGGGGGTAAK (triply charged) Protein: At1g73590Score:74
060619_sm3001_39 #2979 RT: 40.71 AV: 1 NL: 2.40E4T: ITMS + c NSI d w Full ms2 [email protected] [ 310.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1130.7258
987.5266
1201.3008950.7074
745.11651520.5453
1093.57191601.9991
850.66821357.4640 1688.7260
1900.83251250.2773543.3792448.3607 675.4132 1799.1379 1965.9025
y162+
y182+
y12
b202+
y192+
b13-18
[M+3H+-98]3+
[b25-98]2+
b16
b15
y18-98
b18 b20
b232+
GPTPRPpSNYEEDGGPAKPTAAGTAAGAGR (triply charged) Protein:At1g73590Score:64
060619_sm3001_19 #2273 RT: 34.45 AV: 1 NL: 1.88E4T: ITMS + c NSI d w Full ms2 [email protected] [ 250.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
912.9224 1000.5012
706.1671
860.4427
827.8031
1325.49411031.1826
1423.5624731.6096509.4018675.4318
1163.0056 1607.6488564.7716481.2579 1705.6223283.1243 1913.5353
[M+3H+-98]3+ y12
y17 2+
Y192+
y9b7-98
y7
b5
y22 2+ b12-98
b13-98
b12
b14-98
b16-98b16
SDAVWDEPTpSPKProtein:At1g76070Score:54
051114_sm2013_27 #2625 RT: 32.99 AV: 1 NL: 1.68E4T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-1425.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
1039.3566
853.2858
373.0942 609.3479 738.4299274.0980
559.1682
673.96151138.4297
344.9702520.3168
803.2987
389.1833 871.4733256.3924 1070.3914 1209.52671306.9185
y7
y5b4b3
b9
y9
y8
y9-98
y6b5
y10
SEADLDSQVVDFQpSPKProtein:At1g76660Score:92
051114_sm2013_12 #3107 RT: 36.16 AV: 1 NL: 8.12E3T: ITMS + c NSI d w Full ms2 [email protected] [ 240.00-1860.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
801.2692
999.3447
1214.37991329.4423873.9924
686.30071044.2823
1434.4150
1159.4189411.2032 945.2738
631.19961503.2891588.3484
385.14241601.4800
1687.6243
y6
y5
y3
y5-98
b10
b8
y8
b9
y13y12
b11
y11
Dp(TT)PVAAIGKProtein:At1g77110Score:50
060718_sm3007_31 #2131 RT: 40.24 AV: 1 NL: 1.25E3T: ITMS + c NSI d w Full ms2 [email protected] [ 135.00-1065.00]
200 300 400 500 600 700 800 900 1000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
369.8604
655.4219
468.8776
398.1385
836.3174
204.1569317.2890
751.2083
638.1324491.2401 738.4608849.2008282.1383 549.2483 947.4008189.1618 818.3729
707.3434 867.2356 995.7072
y7
y8
b3
y3
b5
b2y2
[y8-98]2+
[y9]2+
b8-98
ApSWDGSLIGRProtein:At3g09070Score:45
051114_sm2013_29 #2822 RT: 35.18 AV: 1 NL: 1.18E4T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1155.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
522.3370
812.2902
602.3953
717.4124232.2487
1000.4869
442.1964345.1918
699.3717586.0064 755.1985 909.9969681.1676327.3040
289.2527 427.4305 937.4443215.0527 869.0844666.49831070.3086
y7
y6y5
y4
y3
y2
y8
[M+2H+-98]2+
b8-98
b4-98
b3-98
QpTPSVQIPRProtein:At4g24630Score:57
060619_sm3001_25 #2114 RT: 39.41 AV: 1 NL: 7.62E3T: ITMS + c NSI d w Full ms2 [email protected] [ 140.00-1120.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
495.4948
272.1856
513.3362
817.3289
796.5045385.3219291.0966
459.0719
233.0977 719.2185350.1544 558.1815 606.3390
194.0175 736.5723686.1459 834.3588 913.3766 971.3137 1067.2570
y7
y5
y4
y3
y2
b5-98
NAISpSPYIDPRProtein:At4g32410Score:53
060718_sm3007_33 #4353 RT: 55.10 AV: 1 NL: 3.34E3T: ITMS + c NSI d w Full ms2 [email protected] [ 170.00-1325.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
272.1621 1041.2189
760.4889
299.2747 599.0434
925.4407810.3002387.3495
828.3049 1127.3334
564.2943
943.3036
697.3140254.2392 437.1281
507.9222240.1103 368.2513 1177.7780
y5
y2
y9b5-98
b3
y3
y4b4
y6
y10 2+
Y92+
b8-98
b9-98
QDTSGEFSAApSPERProtein:At5g05170Score:75
051114_sm2013_29 #1725 RT: 26.34 AV: 1 NL: 4.24E3T: ITMS + c NSI d w Full ms2 [email protected] [ 205.00-1575.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
723.7164
944.3575
797.3180
568.2409
639.2485401.3075
888.1833541.3789977.3091
470.4284 1130.3733846.4526 1318.4872994.3217375.1396 1272.6725 1352.4729243.9968 1545.1158
y4
y7
y8
y9-98y3
y6-98
y5
y6y5-98y4-98
y10
y11
pSVGSPAPVTPSKProtein:At5g12080Score:85
060619_sm3001_03 #1406 RT: 32.92 AV: 1 NL: 2.23E3T: ITMS + c NSI d w Full ms2 [email protected] [ 155.00-1220.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
554.8397 796.5305
313.1917
432.2610 628.4177
940.6050331.2274481.2092
411.0941 883.5178677.3910
760.4531226.1441
858.2482699.4471
962.4202 1060.3737
b3-98
b4-98
y3
[M+2H+-98]2+
y4
y8
y6
y10y9
GGLpSPVIVPRProtein:At5g39865Score:54
060619_sm3001_25 #2827 RT: 44.35 AV: 1 NL: 4.31E4T: ITMS + c NSI d w Full ms2 [email protected] [ 135.00-1090.00]
200 300 400 500 600 700 800 900 1000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
488.9150
371.2582
606.3550
705.4130272.2810
431.8226297.1233 583.5042 847.5957175.1422 247.1924 749.4888 900.2242656.9475563.2560 960.4032
y8
y7
y6
y5
y4
y3
y2
[M+2H+-98]2+
b8-98
b7-98
b6-98
b4-98
b9
QpTPTVQIPRProtein:At5g50020Score:52
060619_sm3001_23 #2188 RT: 39.85 AV: 1 NL: 1.59E4T: ITMS + c NSI d w Full ms2 [email protected] [ 140.00-1135.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
502.4846
272.2178
810.5769
831.4207385.3065 571.9674
405.7445
733.3195612.4048
718.2043 750.3939175.1870 292.0111 447.3485 700.3108
879.4602 985.2190 1081.7104
y7
y4
y2
[M+2H+-98]2+
b82+
[y8-98]2+
y72+
y5
y6
y3
TLpSPKPPSPR (triply charged)Protein:At2g43680Score:42
060619_sm3001_47 #1865 RT: 33.34 AV: 1 NL: 5.98E3T: ITMS + c NSI d w Full ms2 [email protected] [ 95.00-1175.00]
100 150 200 250 300 350 400 450 500 550 600 650 700 750m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
473.37
424.41
277.32
553.37479.33
187.11
215.15 464.34354.73 429.97304.13255.28
348.72 587.39530.01
226.24175.22 389.92309.81129.18 607.21511.99 671.45633.35 696.92 761.68
y82+
y5
[y8-98]2+
y52+
y72+
b62+
y3
b2
[b5-98]2+
b52+
FGSAPTTAASRpSPEMKProtein:At5g67470Score:66
060619_sm3001_15 #1998 RT: 34.08 AV: 1 NL: 1.71E4T: ITMS + c NSI d w Full ms2 [email protected] [ 225.00-1730.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
810.6473
629.53141116.5833
678.6021
504.2530
757.55901257.6351942.3363
1342.5500
887.4031278.2602 1440.51061047.6672 1214.5991
345.0822 573.4274427.1852 1473.6476
1631.4537
[M+2H+-98]2+
y4
y3
y2
y122+
y112+
y5-98
y142+
y132+
y8-98
y8y7
b12-98
b11
y12-98
b14
b14-98
IPNpSGNLVFCGESRProtein:At5g67200Score:105
051114_sm2013_24 #2944 RT: 34.97 AV: 1 NL: 4.83E3T: ITMS + c NSI d w Full ms2 [email protected] [ 210.00-1645.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
766.5818
710.0303
854.3328608.3397
1138.4767967.3912
661.3588 1321.4861262.1081 448.3474 548.2428
1022.3022924.5007 1207.3713 1368.4955394.1992
1500.2377
y10
y8
y7y5
y3y2
y9
[M+2H+-98]2+
y6
y12-98
y11-98b4-98
QNpSENVLIDMEKProtein:At5g61740Score:65
060718_sm3007_11 #2254 RT: 35.78 AV: 1 NL: 5.96E4T: ITMS + c NSI d w Full ms2 [email protected] [ 195.00-1530.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
538.2863
700.5994
651.2505
961.2144848.2239
423.1921 977.5516474.2545
880.3428764.43371075.4310
359.3125 1273.45901175.3073 1369.3494295.1885
y4
y6
y5
y3
b7-18
y7
y8
b9 b11
AVpTPSSSLEFRProtein:At5g41620Score:66
051010_sm1096_19 #3024 RT: 43.61 AV: 1 NL: 8.44E3T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1285.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
503.3150 922.4531
588.4150
352.1403
322.2654 1103.4297
651.4308254.1045 738.5038451.3366 552.2794825.3405
952.2760789.6014 1085.3541666.6796369.1749194.1402 1132.8645
y8
y7y6
y5y3
y2
y9
b3
y9-98
[y9-98]2+
SVGpSPAPVpTPSKProtein:At5g12080Score:67
060619_sm3001_23 #1432 RT: 32.66 AV: 1 NL: 9.75E2T: ITMS + c NSI d w Full ms2 [email protected] [ 155.00-1220.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
554.8259
461.8964
796.4098
331.2159628.4252
432.27841020.4624
778.3716
481.2130
858.3180313.2437
677.3270922.4088
757.3127
699.3516187.1376 414.5898295.0055 1002.2219 1042.0544 1195.9229
y10
y8
y7
y6
y4
y3
Y10-98
[M+2H+-98]2+
b2
VTVMPIIpSPERPVKProtein:At4g03390Score:75
051010_sm1096_40 #3454 RT: 44.71 AV: 1 NL: 3.45E5T: ITMS + c NSI d w Full ms2 [email protected] [ 215.00-1660.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
774.6823
608.57531215.5458
559.6367
1117.6602
725.5435
892.4351674.6259 1303.6001
1043.5728499.4152
343.4521929.5826
455.28681346.7252 1499.6414
1653.3950
y11
y10
y8
y7
y6
y4
y3
y132+
y102+
y92+
y8-98
b11
y10-98
FDWDHPLHLQPM*pSPTTVK (triply charged)Protein:At3g29360Score:42
051114_sm2013_59 #3295 RT: 37.04 AV: 1 NL: 2.25E4T: ITMS + c NSI d w Full ms2 [email protected] [ 195.00-2000.00]
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
716.7598
645.3155802.1024
956.3046545.3334
479.0293858.3400 1289.42081048.4902
1161.4105429.9077
246.33701335.5590 1594.0851 1700.3276
y5
y82+
b102+
b4
b92+
[M+3H+-98]3+
y72+
[b13-98]2+
y8-98
y7
y8
b8b10
b9
TV#NGGM*SpSPSLQKProtein:At3g48760Score:97
051114_sm2013_26 #1559 RT: 23.91 AV: 1 NL: 8.31E2T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-1415.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
601.5202
572.2853
652.4377
543.56091202.4561
1087.5416739.3225
826.40161030.4584
486.9364275.2163 973.3153 1238.2660388.3693 1110.6213906.8080
258.1608 794.7780 1370.8605
y11
y10
y8
y7
y6y3y2
y9
y5
y102+
y82+
y112+
SVAGGFMpSPNMGKProtein:At3g26935Score:99
060619_sm3001_23 #2779 RT: 43.79 AV: 1 NL: 2.10E3T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-1375.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
588.7292
844.2747539.7880713.3279
1105.3789
1176.3623
1048.3311
746.3252501.1654
991.4574354.2096
650.1241
950.3507305.2590204.2243 473.1793240.0641 795.3719 1216.2833
y7
b5
y3
y2
y10
y11
y8
y10-98
y11-98y8-98
y9-98
y9y7-98
y6
y5
y4
SLFLSSTSApSPARProtein:At3g07540Score:83
051114_sm2013_20 #2912 RT: 33.95 AV: 1 NL: 4.99E3T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-1415.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
943.3309
510.2235 653.2079
668.3083
856.3209
769.3066
581.2089
820.3433907.3195
1056.4088
343.3101
461.0713963.3093
412.3571303.1085 736.2841 1203.66281229.2278
246.1117 1131.6980 1264.8832
y11
y10
y8
y7
y6
y5
y4
y3
y2
y9
y4-98
Vp(SSS)PTASPTFVSTPKProtein:At2g33490Score:57
051008_sm1096_08 #2685 RT: 40.79 AV: 1 NL: 2.96E3T: ITMS + c NSI d w Full ms2 [email protected] [ 220.00-1685.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
778.9088
963.5011
876.4531
686.1763
1232.6292
651.65061034.5061594.3012
1095.4586495.3265
1135.54411370.3777379.2645
244.3385 1497.72231583.7726
y14
y12
y11
y10
y8
y7
y5y4
y3
y2
y9
p(TS)FSAADELFDGGKProtein:At2g15760Score:73
051008_sm1096_10 #4075 RT: 50.79 AV: 1 NL: 6.80E3T: ITMS + c NSI d w Full ms2 [email protected] [ 200.00-1540.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
713.9332
691.8793
880.4111
951.4260
529.37321022.3456 1109.5282
636.4279376.1651
618.4154458.1467 773.3944261.2092 1256.41821353.4340318.2112
1433.4160
y12y11y10
y8
y7
y6
y5
y4y3
y9
[M+2H+-98]2+
GLDIDTAGHHYpTVProtein:At2g18960Score:48RAWfile:sm3007_37_msScanNumber:5323
060718_sm3007_37 #5323 RT: 61.49 AV: 1 NL: 1.99E3T: ITMS + c NSI d w Full ms2 [email protected] [ 190.00-1490.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
690.9748632.3904
590.8068
1017.4010
982.3108
1080.3185
867.4485793.2544509.3118577.3386 766.3370
286.2013 1180.4574371.3474912.5886301.3156 399.1901
1162.6176229.1458
b3
b4y4--98
b102+
y7-98
y6
y7
y8-98y8
y9-98b10
b11
y9
b112+
[b11-98]2+[M+2H+-98]2+
[b12-98]2+
GSEGQTPPGEpSRTPPR (triply charged) Protein:At2g26730Score:44
051114_sm2013_48 #1509 RT: 24.36 AV: 1 NL: 1.47E4T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1750.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
638.0034
730.5157
587.4910
538.5967
518.5325
369.2989
397.2892786.0793
256.2020922.1305 1091.6661 1174.6481
b3
b3-18
y3
y62+
[y8-98]2+
b5
y102+
y92+
y112+
y142+
y132+
y122+
GLTPVSEpSPRVSpTKProtein:At1g78610Score:55
051114_sm2013_33 #2384 RT: 30.08 AV: 1 NL: 1.29E5T: ITMS + c NSI d w Full ms3 [email protected] [email protected] [ 195.00-1535.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
711.5712
624.9762
576.0560
1052.4338
1248.51681150.6776836.3893317.2742 526.8618 965.4426450.3189 1272.4969
[M+2H+-98]2+
y3
y2 y4
b5-18b4-18
y102+
y92+
y112+
y6
y7
y9
y8
y9-98y8-98y11-98 y10
y12-98
b11
y11
QDQSpSPINFEMSSRProtein:At5g40300Score:72
060619_sm3001_07 #2448 RT: 42.82 AV: 1 NL: 4.45E3T: ITMS + c NSI d w Full ms2 [email protected] [ 220.00-1720.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1080.4023
795.3671
870.3523
609.4386
756.3588
1218.5483480.2675
349.3033 1334.5471510.1882
1247.4896703.1649
933.37951149.5645441.2568262.3335 1444.4054983.5393
1531.0499
y12
y11
y10y8
y7
y6
y5
y4
y3
y2
y9
APEYALVTQNpSDPTSPRProtein:At4g08330Score:87
060718_sm3007_11 #2525 RT: 37.39 AV: 1 NL: 4.71E3T: ITMS + c NSI d w Full ms2 [email protected] [ 255.00-1940.00]
400 600 800 1000 1200 1400 1600 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1182.4407
637.31841289.4573
905.4276
744.2582
539.3767
1064.43921394.5745855.4202 1556.3702495.3853
1465.5496973.4618461.2217
341.4048 1628.56531751.4419 1854.5033
y3-98y3
y2
b4
y4
y5
b7
y7
y8-98
b9
y10-98
b10
y10
y11-98
y11
b12
y12 b15-98
y13
y14
GEDKDVEAGp(TSS)FTEYEDSPFDIASTK (triply charged)Protein:At4g29900Score:97
060619_sm3001_13 #4304 RT: 48.01 AV: 1 NL: 7.54E3T: ITMS + c NSI d w Full ms2 [email protected] [ 265.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
964.0731
878.4529
1080.4895
1372.60601209.5000
844.2620
644.37241327.1685
406.2838
519.3662 1501.6132
335.3287 1633.5277
1761.79211929.0022
b5
y5
y4
y3
b8
y8
b7
b6
y6
y10
[b17]2+
y9
y11
y12
b13
y14
y13
b15
y15
HTDp(ST)VPVFNLDDIEEGVEGAGFDDEK (triply charged)Protein:At5g05170Score:78
060619_sm3001_39 #5316 RT: 56.64 AV: 1 NL: 3.60E2T: ITMS + c NSI d w Full ms2 [email protected] [ 265.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1148.1648
967.2676
710.4059838.3524
1435.31681255.73241089.9617
781.3909 1370.7102623.2349
1764.5217391.2594
1635.8218506.4039
1863.6693
b6-98
y5
y3
y8
b7
y9
y6
Y202+
y202+
b232+
y12
b262+
b252+
b242+
b15
b14
y13
y11
051010_sm1096_27 #3064 RT: 44.84 AV: 1 NL: 1.58E4T: ITMS + c NSI d w Full ms2 [email protected] [ 270.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
975.00
1191.32
907.46
1093.48839.32
505.33 822.20576.451404.52
1290.40691.43 1687.531444.37448.35 1774.61626.26415.10
ADNNNNNVDAFpSDAGWSR Protein: At1g05150Score: 86
y3
y4
y5
y6
y7-98
y7
y8-98
[M+2H+-98]2+
y9-98
y9
y10
y10-98
y11
y12
b16-98b17-98
051114_sm2013_30 #3023 RT: 35.61 AV: 1 NL: 1.01E4T: ITMS + c NSI d w Full ms2 [email protected] [ 185.00-1460.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
676.06
908.36
697.42
795.28
810.47
1007.42
571.35618.29
523.22
505.15
407.08
738.35379.23304.21 1047.47 1274.391120.28 1176.54837.37286.18 935.25 1377.74
SSGPIVLGpSGPIER Protein: At1g07630
Score: 53
y9
b12-98b13-98
y8
y8-98
y7
y7-98
[M+2H+-98]2+
y6
y5
y2
y4 y9-98
NM*EDIFNTSpSR
051114_sm2013_29 #2440 RT: 31.60 AV: 1 NL: 4.69E3T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-1425.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
647.44 791.30
638.66
656.44619.15
506.18
904.38
1019.42
1130.39766.20530.39
1148.33432.44 600.28313.20 749.35489.09262.22 546.35 964.29331.19 880.271032.60
391.12 1050.60244.48
846.30731.33236.09 1209.33 1305.43
Protein At1g15210
Score: 64
y9
y8
y7
y6[M+2H+-98]2+
y5
b5
y4
b4
y3-98b2
y4-98
y2-98
VSGPLDpSSGLMK
051114_sm2013_17 #2395 RT: 30.17 AV: 1 NL: 2.39E3T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1300.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
551.05
594.60
522.58
464.48833.30
719.23
603.25
1044.45 1101.36617.43 735.39452.04383.22 985.58946.47327.13 1140.80654.17426.16268.23 877.51 1188.58742.68225.93
Protein: At1g16860
Score: 71
y11b11
y10
b11-98\ y9
y8
y7
y7-98
y6
y5
[M+2H+-98]2+
y92+
y4
SLpSINLMK
051114_sm2013_20 #2765 RT: 32.75 AV: 1 NL: 1.10E5T: ITMS + c NSI d w Full ms2 [email protected] [ 125.00-1015.00]
200 300 400 500 600 700 800 900 1000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
452.40 801.23
352.35
703.30
521.29201.07173.11 443.58
634.32383.06 469.45
757.32294.29
407.27270.19 595.14343.56 739.29 855.18
474.77147.01 320.60 557.25 686.32 925.16252.81 873.41
Protein: At1g18890
Score: 47
b7
y6
b7-98
y6-98
y5
y4
[M+2H+-98]2+
y3
b4-98
[y6-98]2+
y2
b2
a2
051114_sm2013_59 #3347 RT: 37.40 AV: 1 NL: 4.88E3T: ITMS + c NSI d w Full ms2 [email protected] [ 170.00-2000.00]
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
634.27
592.45
717.45518.72
746.73454.29
315.27882.38 1108.34
1183.79
364.31261.05 864.44
1036.51931.171491.591272.41
1600.871453.15
HGVpSEHYDEFPLVFNK(triply charged)
Protein: At1g27190
Score: 47
[M+3H+-98]3+
b12
b10-98
y9[y15-98]2+
or [ b13-98]2+b122+
y6
[b10-98]2+
[b9-98]2+
[b8-98]2+
y2
b62+
KEEFLIGSIEEESQSQpSPR
051010_sm1096_48 #3448 RT: 46.90 AV: 1 NL: 1.96E4T: ITMS + c NSI d w Full ms2 [email protected] [ 300.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
11.0
11.5
12.0
12.5
Re
lative
Ab
un
da
nce
1088.36
1513.49
1415.55760.35
1904.66
1238.521017.47869.35
1626.59
1528.651275.46 1886.70
556.43 999.57654.31 1158.45817.43
742.60 905.561834.71
439.20 732.361740.57629.59
387.441984.80538.37
Protein: At1g53590
Score: 85
b17-98
b16y15
b14 y14
y13
y13-98
b12
y11
b11
y10
[M+2H+-98]2+
y10-98
b9
b8
y7
b6
y5-98 y5
y3
y3-98
ASSGVVVVGESpSVTK
051114_sm2013_21 #2351 RT: 30.88 AV: 1 NL: 2.35E4T: ITMS + c NSI d w Full ms2 [email protected] [ 190.00-1500.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
787.27
693.53
985.34
886.38
1084.36
685.48
1283.35
600.26 712.28593.34
972.28395.37501.25
954.36582.25494.29248.25 1140.41769.17 1339.46868.49 1070.10 1238.41366.36313.06 849.30
1392.27
Protein: At1g54610
Score: 45
b14y12b13
b13-98
y10
y9
y8
y7
[M+2H+-98]2+
b7
y4
b6b5
y4-98
y2
AAAASSWSDNSGTESpSPR
051114_sm2013_26 #1831 RT: 26.02 AV: 1 NL: 7.23E3T: ITMS + c NSI d w Full ms2 [email protected] [ 245.00-1875.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1216.36
872.99
813.29900.29
1402.38
1129.41
1014.401422.33
1317.381048.28
1489.44 1576.55795.28526.20 627.20996.34756.41
439.27 655.28
469.10 556.22398.11 714.35 1286.51
1718.66
Protein: At1g59610
Score: 123
y2
y3
y4
y5
y6
y7
[M+2H+-98]2+
y8
y9
y10
y11
y12
y14y13
NIEDIFSSGpSR
051114_sm2013_26 #3108 RT: 37.22 AV: 1 NL: 4.98E3T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1320.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
720.25
573.25
1077.39
603.72
1059.26948.31
732.27
979.36
833.36622.30486.21
819.36200.11
388.40301.29502.15
357.32 850.53399.15 802.28 1041.321200.42702.28455.32253.66 758.20 1130.43
Protein: At1g59870
Score: 68
y9-98
y9
y8-98
y8
y7
b6
y6
y6-98
b5
y5
y4
y3
y2
y3-98
TQpSVNDDEEALK
051114_sm2013_05 #2002 RT: 26.27 AV: 1 NL: 2.97E3T: ITMS + c NSI d w Full ms2 [email protected] [ 185.00-1440.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
665.94
933.47
1199.30
1101.53
639.37
374.14 488.30398.26 679.40
299.23
1032.54589.42 871.31819.33460.47
230.12725.40522.81 1184.50356.20 1264.47
1000.52
Protein: At1g59870
Score: 60
[M+2H+-98]2+
y10
b2
b3-98
y10-98
b10-98y9
y8
y7
y5y4
b3
b4-98
VLPQEAPSLHQVEVGQp(TS)PR
051010_sm1096_37 #2889 RT: 41.71 AV: 1 NL: 2.16E4T: ITMS + c NSI d w Full ms2 [email protected] [ 300.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
1068.79
1020.70
962.77
1614.70
949.80
1882.741527.72
1299.56
725.36 935.50 1317.53
1428.55638.37 824.52 1685.861163.47583.44426.38 1864.98355.29 1980.731332.72
Protein:At1g68720
Score: 71
b17 or y16
b18-98
y15
y14
b14
y14-98b13
b12
y11
[M+2H+-98]2+
y182+
y172+
b9
y7
y6
b6
051010_sm1096_40 #3913 RT: 47.68 AV: 1 NL: 8.25E4T: ITMS + c NSI d w Full ms2 [email protected] [ 275.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
987.22889.63
853.431163.45
1379.601278.43
1016.34
759.01635.37
750.05504.36815.94433.35
1236.431138.17 1517.03612.36 1630.72713.30 1102.38346.31 1778.581656.23 1914.50
Gp(SS)FNHTDFYSMMASGGGR Protein: At1g73590
Score: 78
[M+2H+-98]2+
y16
y15
y14
y13y12
y11
y10
y9
y8
y7
y6y5
y4
LGGDGSApSPTASTVR
051114_sm2013_27 #1788 RT: 27.29 AV: 1 NL: 2.55E4T: ITMS + c NSI d w Full ms2 [email protected] [ 190.00-1470.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
Re
lativ
e A
bu
nd
an
ce
679.57462.36
871.43
661.35
898.331113.47
609.27 969.37
594.45
731.48
800.45533.32 1015.54
375.38
426.32 1056.481183.45
274.22 1263.32
1281.39
Protein: At2g17480
Score: 78
y14-98
y11
b14-98y10
y11-98
y9
y8
y9-98
y8-98
y7
[M+2H+-98]2+
y5
y4
y3
y2
pSGPLAATTSAATR
051114_sm2013_11 #2134 RT: 27.49 AV: 1 NL: 9.41E2T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1295.00]
200 300 400 500 600 700 800 900 1000 1100 1200m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
408.19
593.39778.61
707.19
506.20
606.33 760.43479.12
849.47
577.17
425.53 910.01530.42347.17
919.24335.51 671.39224.34 972.68380.01259.28 841.68 903.46830.83642.70
Protein: At2g23140
Score: 50
[M+2H+-98]2+
b5-98
y6
b8 or y8
y7
b11-98
y9
b6-98
b5 or y5
VDNNNVDAFSEAGWpSR
051010_sm1096_21 #3109 RT: 46.09 AV: 1 NL: 1.09E4T: ITMS + c NSI d w Full ms2 [email protected] [ 245.00-1875.00]
400 600 800 1000 1200 1400 1600 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
882.16
1205.40
1019.34656.32
1090.36774.49
487.36972.41558.34 1304.39
754.35 1418.37430.29 1501.43342.24
1674.92 1772.60
Protein: At2g32450Score: 77
[M+2H+-98]2+
y11-98
y10
y11
y12
b14-98
y10-98
y9
y8
y7-98
b6
y5
y4
SGLFMpSGPIER
051114_sm2013_29 #2750 RT: 34.47 AV: 1 NL: 1.22E4T: ITMS + c NSI d w Full ms2 [email protected] [ 165.00-1305.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
596.45
885.30516.77738.32 787.40
571.33
304.201032.29467.73
613.24 934.41405.12 870.46258.06 678.21 986.41 1115.23377.24213.11 1212.76
Protein At2g35350
Score: 43
b10
y8
y9-98
y7
[M+2H+-98]2+
y7-98y6
b6-98
b7-98
y5
y82+
b4
y2
FTHTQVIGM*TSGSp(SS)R
051114_sm2013_51 #2137 RT: 27.53 AV: 1 NL: 4.14E4T: ITMS + c NSI d w Full ms2 [email protected] [ 235.00-1805.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
838.57
806.53
965.25
714.35
864.451078.31797.63
980.40
696.35
1177.31615.38
1031.30947.27 1406.411132.45 1308.46
475.31 562.471363.67349.25 400.31 487.08
331.27 1501.73 1599.37
Protein: At2g36910
Score: 87
y3-98
y13
b13
y5
y13-98
b11
y11
b10
y10
b9
y10-98
y9
[M+2H+-98]2+
[y14-98]2+
[y14-(98+18)]2+
b5
y6-98
GASPQAISSKPPpSPR
051114_sm2013_47 #1756 RT: 25.22 AV: 1 NL: 3.23E5T: ITMS + c NSI d w Full ms2 [email protected] [ 200.00-1575.00]
200 400 600 800 1000 1200 1400m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
Re
lative
Ab
un
da
nce
731.66633.27
935.36
673.07535.38
623.90
1190.39837.41272.21
1288.35
799.27 848.37 1048.62439.17 1119.56
341.31 950.50
1345.53
Protein At2g43680Score: 69
y3-98y3
y2
b13
b13-98
y10
y9b9
y8-98
y8
[M+2H+-98]2+y5
y122+
y4
GASPQAISpSKPPpSPR
051114_sm2013_36 #1888 RT: 27.17 AV: 1 NL: 1.10E4T: ITMS + c NSI d w Full ms2 [email protected] [ 215.00-1655.00]
400 600 800 1000 1200 1400 1600m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
Re
lativ
e A
bu
nd
an
ce
771.39713.36
535.31
1270.451007.24272.21
917.37
505.39 882.30
830.331172.56 1368.13439.39
1154.38341.20
1425.06 1527.17
Protein At2g43680Score: 47
y3-98y3
y2
y5
y4
b14-196
y10 b13b13-196
b8
y122+
b10
y8
y7
LDAPRPp(TT)PKPPpSPRTriply charged
051114_sm2013_48 #2167 RT: 28.47 AV: 1 NL: 2.22E6T: ITMS + c NSI d w Full ms2 [email protected] [ 150.00-1805.00]
200 400 600 800 1000 1200 1400 1600 1800m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
564.79
711.06
521.47272.18
633.29 1157.46834.45465.26
429.80 781.50 858.44226.11 1210.471059.41
Protein: At2g43680Score: 42
y5
y2
[M+3H+-98]3+
[b14-196]2+
b8-98 b10
SLSPKPPpSPR
051114_sm2013_56 #1655 RT: 24.84 AV: 1 NL: 3.56E5T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1160.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
Re
lative
Ab
un
da
nce
524.52
272.19
776.32515.49
424.46
473.42
633.25
535.36380.93
858.46
226.12760.41
847.59945.44175.08 295.33 663.09542.77 840.36 1010.41
Protein: At2g43680Score: 37
y8
y2
y3
y1
[M+2H+-98]2+
y5-98
y5
y7
ALAVVEKPIEEHpTPK
051114_sm2013_56 #2092 RT: 27.67 AV: 1 NL: 4.78E4T: ITMS + c NSI d w Full ms2 [email protected] [ 225.00-1755.00]
400 600 800 1000 1200 1400 1600m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
822.12
1030.37
813.09
932.42
1399.58700.521158.35 1288.51583.28 800.35355.21
664.53 837.72454.22 1497.41283.26 1571.69
Protein: At2g45820Score: 60
b13-98
y11-98y9
y8
y8-98
[M+2H+-98]2+
[b13-98]2+
b6b4
y3
VDVEpSPAVLAPAKEPTPAPVEVADEKTriply charged
Protein: At2g45820
Score: 88
060619_sm3001_33 #2991 RT: 43.92 AV: 1 NL: 9.96E3T: ITMS + c NSI d w Full ms2 [email protected] [ 240.00-2000.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
840.18
881.13
1252.631054.52
1065.38779.38
931.90
1148.77
981.39
528.08
695.24 1204.77626.89
755.70 1468.59426.451100.16561.26479.34 1382.58
1586.84408.44297.44 1278.61
b5-98y10
2+
y122+
[b14-98]2+
b7-98
b8-98
[M+3H+-98]3+
y182+
y10
y222+
y12
b14
TNpSLLVLPR
051114_sm2013_21 #3262 RT: 37.31 AV: 1 NL: 7.94E2T: ITMS + c NSI d w Full ms2 [email protected] [ 140.00-1105.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
385.26
484.33
511.17
610.27
597.34
272.19723.24
397.90519.08
877.63
779.48821.21
592.14457.98285.10 710.44444.25 943.08370.17 759.95215.96 321.91175.13 826.92628.44
927.32 959.14
Protein: At3g05200
Score: 68
b4-98
y4
b2
y2
y3
b8-98
b7-98
b6-98
b5-98
y7
y7-98
FEESSIDDLSGVp(SM*S)K Protein: At3g46290
Score: 69
060619_sm3001_03 #2497 RT: 42.33 AV: 1 NL: 3.26E3T: ITMS + c NSI d w Full ms2 [email protected] [ 240.00-1840.00]
500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
864.87
855.84
693.27 791.23 1134.35
1036.48548.22 704.28 1279.46 1383.55675.24606.27
1261.36806.39 877.28 1019.40450.27 717.63 1334.661105.37657.37481.49 1421.55 1582.25580.49 1149.221054.44 1495.43923.27 1000.35
[M+2H+-98]2+
y4-98
y4
or y15-98
b15-98
y13
y12
b12
y10
or y10-98b9
or y7-98
b6
y7
HGGFAYM*YFDDDEpSHKTriply charged
051114_sm2013_63 #2937 RT: 34.08 AV: 1 NL: 2.07E5T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-2000.00]
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
639.84
618.48
780.28
943.33
925.13
827.36470.28 1072.29390.65
1137.39712.38 862.23
569.37356.79 1235.21
348.181321.50
1430.61 1631.99 1707.08
Protein: At4g13510
Score: 68
y9
y9-98y8
[M+3H+-98]3+
y7
y7-98y8-98
[y9-98]2+
b5
051114_sm2013_14 #2519 RT: 31.54 AV: 1 NL: 4.27E4T: ITMS + c NSI d w Full ms2 [email protected] [ 115.00-940.00]
150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
414.35
632.38365.22 660.32
730.35316.79 563.42197.11
827.22405.89330.25 427.06175.10 526.31 624.34 752.19266.18 464.06 713.59217.05 793.26 839.40
VPpSFIQR Protein: At4g19930
Score: 61
[M+2H+-98]2+
b2y1
y2
y4
y3y5-98
y6
INpSGALSATM*SPK
051114_sm2013_06 #1831 RT: 28.55 AV: 1 NL: 3.05E3T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-1385.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
637.7730
737.3344
850.4143
538.2628
425.2190
978.3721
1047.3392 1145.5034921.4037331.2648
228.0681 1270.2595
Protein: At5g48800Score: 76
y10
y9
y8
y7
y6
[M+2H+-98]2+
b6-98
b5-98
y5
y3
INSGALSATMpSPK Protein: At5g48800Score: 89
060718_sm3007_33 #3532 RT: 48.91 AV: 1 NL: 4.44E4T: ITMS + c NSI d w Full ms2 [email protected] [ 175.00-1370.00]
200 300 400 500 600 700 800 900 1000 1100 1200 1300m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
801.29
914.33
630.00
1129.36
621.05
643.26 714.22
556.28
1042.31
411.21
538.30426.14
696.25 985.39443.15 613.44896.37783.23313.08 1111.31928.31398.12228.18 512.24 816.46
297.01 381.11 1243.27494.39 887.55 1093.60765.30 1156.39
y12
y11
y10
y9
y8
b8
y7
or y6
[M+2H+-98]2+
b7
or y5
y3
b2
GLDIETPpSHYTV
051010_sm1096_12 #3668 RT: 47.56 AV: 1 NL: 5.54E4T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-1425.00]
200 400 600 800 1000 1200 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Rela
tive A
bundance
648.06
657.55
639.00 995.36884.32
598.82
1193.42
1030.39528.25 866.43
1126.45783.26286.23 1241.41977.43500.35
382.331294.30219.22 667.38
1312.30
Protein: At4g30190Score: 52
b10
y10
y9
b9
y7
y6
[M+2H+-98]2+
b5
y3
b3
y2
SSPASM*M*pSDPELADSK
051114_sm2013_18 #1868 RT: 27.44 AV: 1 NL: 3.83E3T: ITMS + c NSI d w Full ms2 [email protected] [ 230.00-1780.00]
300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
795.98747.03
1006.18
1531.25
1422.44
420.28 842.03349.31 533.44
737.75 850.661041.27 1380.39
908.18712.08 1188.39 1513.42988.27
663.79 1232.251134.43619.36 926.221618.32541.37515.27
331.24
Protein At5g02620
Score; 61
y5
y4
y3
y7
y9-98
y9
y10
b14y12
b15
NLGSDpSFNSVEM*SR
051010_sm1096_16 #2744 RT: 40.89 AV: 1 NL: 6.00E3T: ITMS + c NSI d w Full ms2 [email protected] [ 215.00-1650.00]
400 600 800 1000 1200 1400 1600m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lativ
e A
bu
nd
an
ce
771.02
538.32
762.15
724.34
657.39
838.46
637.33 1411.44985.39 1152.32474.33409.25
1313.421169.431003.40
968.33573.46262.13 345.25 1464.35
1534.56
Protein At5g03700
Score; 88
y12-98
y12
y11-98 y11
y10-98b10-98
y7
[M+2H+-98]2+
y6
y5
y4
y3
y2
SM*AVSTQVLpSPSLR
051010_sm1096_19 #2884 RT: 42.49 AV: 1 NL: 5.96E3T: ITMS + c NSI d w Full ms2 [email protected] [ 205.00-1585.00]
400 600 800 1000 1200 1400m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Rela
tive A
bundance
737.53
639.30472.33 1167.52
1002.32752.38
933.34851.42
621.30405.14 1080.55
541.40306.061337.50
1422.49
Protein: At5g10470Score: 74
b4
y4
b3 y5-98
y5
[M+2H+-98]2+
b9y7
y6
b10-98
y10
y9
y12
051114_sm2013_44 #4204 RT: 42.45 AV: 1 NL: 4.27E4T: ITMS + c NSI d w Full ms2 [email protected] [ 325.00-2000.00]
400 600 800 1000 1200 1400 1600 1800 2000m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
1172.11 1283.62
958.53
1059.41857.49
1482.53
1384.581041.50 1265.67
873.401616.71
1583.621367.55534.37 726.45 824.30 1729.84 1945.811808.93613.30397.37 1712.87511.43
KQApSSELFSVPIDTPPELVFR Protein: At5g23450
Score: 81
b4-98y17y15
y14
b17-98
b14-98
b13-98
y11[M+2H+-98]2+
b10-98
y8
y7
y4 b7-98b6-98
GAFpSGPLGRPK
051114_sm2013_53 #2452 RT: 29.87 AV: 1 NL: 1.08E5T: ITMS + c NSI d w Full ms2 [email protected] [ 150.00-1180.00]
200 300 400 500 600 700 800 900 1000 1100m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
Re
lativ
e A
bu
nd
an
ce
534.81
724.41470.88
776.49457.35 923.31667.55
612.45397.16
825.57 940.57244.22 345.01306.34226.15 555.07 888.44 1038.69
Protein: At5g47910
Score: 54
[M+2H+-98]2+
b9
y9
y8-98b9-98
y7
y6
y4
y2
ILpSQMLSQK
051010_sm1096_18 #2684 RT: 40.89 AV: 1 NL: 4.54E3T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1155.00]
200 400 600 800 1000m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Rela
tive A
bundance
523.39
466.86684.32
917.45
410.26899.42
362.27
819.33
540.37
750.47622.46
544.35199.14 345.32
311.59 1030.581041.03
Protein: At5g47910
Score: 42
[M+2H+-98]2+
y8
y7
y7-98
y6
b6-98
y5
y3
y4
ILSQM*LpSQK
051010_sm1096_25 #2129 RT: 36.40 AV: 1 NL: 1.07E4T: ITMS + c NSI d w Full ms2 [email protected] [ 145.00-1155.00]
200 400 600 800 1000m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Rela
tive A
bundance
523.45
514.49
917.31
819.40
410.35
227.21 702.29401.26
899.38531.46 684.48 813.26 932.53 1030.53314.23
Protein: At5g47910
Score: 44
[M+2H+-98]2+
y7
b2
y7-98
y5 or b6y5-98
y3 or b4
b3
RPpSPAVR
051114_sm2013_63 #1301 RT: 22.49 AV: 1 NL: 9.06E3T: ITMS + c NSI d w Full ms2 [email protected] [ 105.00-875.00]
150 200 250 300 350 400 450 500 550 600 650 700 750 800 850m/z
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Re
lative
Ab
un
da
nce
382.86254.20
442.27373.96
511.34323.23
609.28
391.88
195.21591.28
343.05
175.21688.35279.23
493.35 554.26237.29 403.21
158.07 544.32 660.20 705.68461.22220.20 803.99
Protein: At5g47910
Score: 39
[M+2H+-98]2+b2
y5-98
y4
y5
b3-98
y1
TSpSAAIHALK
051114_sm2013_53 #1861 RT: 25.90 AV: 1 NL: 5.76E4T: ITMS + c NSI d w Full ms2 [email protected] [ 135.00-1090.00]
200 300 400 500 600 700 800 900 1000m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
Re
lativ
e A
bu
nd
an
ce
481.96396.96
723.39
473.02
446.07
581.27652.43
240.02 553.37932.30508.89
382.14 820.42775.41331.22
703.34 890.31646.36189.05 834.42258.02
949.26 1064.59
Protein: At5g47910
Score: 50
b3-98
b2
[y8-98]2+ [M+2H+-98]2+
b9
y8
y7
y6
y5
LTVDVQpTPPQpSPPVKPARTriply charged
051114_sm2013_36 #2763 RT: 32.82 AV: 1 NL: 1.69E5T: ITMS + c NSI d w Full ms2 [email protected] [ 180.00-2000.00]
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Re
lative
Ab
un
da
nce
627.58
664.99
889.24
578.63 773.24
919.38
724.27939.13
840.59382.78
510.33 1253.511033.30466.53343.28 1228.55994.08246.83
1126.44 1434.471308.64 1702.411604.57
Protein: At5g65660
Score: 58
b5b3
y72+
[M+3H+-98]3+
or y102+
[y11-98]2+
y112+
y152+
[y16-196]2+
y162+
y11