ang ii (+)
DESCRIPTION
Ang II (–). Ang II (+). SA- b -gal. DAPI. Supplemental Figure 1. A. 1. Ang II (+) Ang II (–). *. *. 0.8. *. 0.6. Aldosterone concentration (nM). *. 0.4. *. *. 0.2. *. 0. 1. 2. 3. 4. 5. 6. 7. (days). Supplemental Figure 2A. 80. *. 60. *. SA- b -gal-positive cells - PowerPoint PPT PresentationTRANSCRIPT
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Ang II (+)Ang II (–)
SA-
-gal
D
AP
I
Supplemental Figure 1
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Ald
oste
rone
con
cent
ratio
n (n
M)
0
0.2
0.4
0.6
0.8
1
1
Ang II (+)
Ang II (–) **
*
*
**
2 3 4 5 6 7 (days)
*
A
Supplemental Figure 2A
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Supplemental Figure 2B,2C
B
*
*
*
0
80
60
40
20
SA
--g
al-p
osit
ive
cell
s(%
of
tota
l cel
ls)
0 0.2 0.4 0.8 1
Aldo (nM)
0
16
4
8
12
**
**p21
0
2
4*
*
p53
0
2
4
6 ***
*
p16
0 0.2 0.4 0.8 1
Aldo (nM)
0
2
4
6
*
**
*p27
Fol
d ch
ange
Fol
d ch
ange
Fol
d ch
ange
Fol
d ch
ange
p21
p53
p16
p27
-SMactin
0 0.2 0.4 0.8 1
Aldo (nM)
C
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-SM actin
Ki-ras2A
Ang II+Aldo 0 1 3 (days)
0
*
*
**
3
2
1
4
5
5 7
Ki-
ras2
A(f
old
chan
ge)
Supplemental Figure 3
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Name Sequence
S
AS
S
AS
S
AS
Ki-ras2A-15’- CGAAACAGGCUCAGGAGUUTT -3’
5’- AACUCCUGAGCCUGUUUCGTT -3’
5’- CCGAGAAAUUCGAAAACAUTT -3’
5’- AUGUUUUCGAAUUUCUCGGTT -3’
5’- AAGAAGAAGUCAAGGACAATT -3’
5’- UUGUCCUUGACUUCUUCUUTT -3’
Supplemental Table 1: Sequences for cocktail of siRNA targeting Ki-ras2A
Ki-ras2A-2
Ki-ras2A-3
Supplemental Table 1
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Name Sequence
S
AS
S
AS
S
AS
5’- GCGCCGUACGAGGUAUAAATT -3’
5’- UUUAUACCUCGUACGGCGCTT -3’
5’- AUAGAACGACAACGCUAUATT -3’
5’- UAUAGCGUUGUCGUUCUAUTT -3’
5’- CGAUUAAGAAACGAAUAGGTT -3’
5’- CCUAUUCGUUUCUUAAUCGTT -3’
Scrambled control-1
Scrambled control-2
Scrambled control-3
Supplemental Table 2: Sequences for cocktail of scrambled version of Ki-ras2A-siRNA as control
Supplemental Table 2