FOR THE DETECTION OF BIOFILMS AND CONTAMINATION ON SURFACES

DESCRIPTION..................................................................................................................................................................................................................

Biofinder is a specialized product for the detection of biofilms and surface contamination in the food, pharma-ceutical and restaurant industries to aid in hygiene control; it is especially recommended for open surfaces.

CHARACTERISTICS - APPLICATIONS..................................................................................................................................................................................................................

It reacts to detect the group of microorganisms attached to surfaces, called Biofilms. It immediately reveals contaminated areas by simple visual inspection. It can be applied to the most commonly used surfaces in the food industry, such as stainless steel, polypropylene and epoxy-coated surfaces.

Biofinder has advantages over other methods, with time and cost reduction being the most significant ones. It simplifies monitoring surface hygiene of industrial processes. Its formula and packaging type make it possible to treat large areas. It doesn’t stain or leave residues on surfaces due to its high water solubility which aids in rinsing. Thanks to its simple application and response type, technical staff is not required for handling. It is not considered hazardous to the environment, according to current legislation.

Its Formula is registered in the Spanish Patent and Trademark Office.

PHYSICAL AND CHEMICAL PROPERTIES..................................................................................................................................................................................................................

Viscosity: 300 – 500 cP

pH: 4,5 – 5,5

Appearance: Orange, crystalline liquid

COMPOSITION OF INGREDIENTS:..................................................................................................................................................................................................................

See Material Safety Data Sheet (MSDS).

DIRECTIONS..................................................................................................................................................................................................................

Spray BioFinder gently on surfaces at a distance of 10 to 15 cm and visually inspect the surface thirty seconds after application. A positive reaction with BioFinder immediately produces a foaming reaction in the form of white microbubbles which clearly contrast with the product’s orange original colour and thus becoming easily recognisable. The reaction is visible for at least 10 minutes, which is clearly due to its viscous nature. A negative reaction is the absence of production of microbubbles after a lapse of one minute, indicating no contamination on surfaces. Finally, rinse the surfaces with abundant water.

RECOMMENDATIONS..................................................................................................................................................................................................................

Do not dilute the product for its use. Do not shake. Apply on surfaces after cleansing and disinfection. In case of a positive reaction, repeat sanitation procedure in the affected areas. Avoid exposure to direct sunlight and high temperatures (> 55 ° C) to prevent thermal decomposition.

HANDLING AND STORING:..................................................................................................................................................................................................................

Store below 20 °C.

WARNINGS..................................................................................................................................................................................................................

In case of contact with eyes, rinse immediately with plenty of water for several minutes. If eye irritation persists: seek medical advice/attention. See product safety data sheet for more information.

PACKAGING..................................................................................................................................................................................................................

500 ml in poly bottle. This

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ENVIRONMENT FRIENDLY PRODUCT

An effective tool for the control of hygiene

The innovative solution for the detection of biofilms and contamination on surfaces

The presence of biofilms in industrial working surfaces within processing operations is the main cause of contamination of the final product.

Biofilms are groups of microorganisms attached to surfaces which produce a number of extracellular polymeric substances (EPS) that protect them from adverse environmental conditions.

EPS make microorganisms resistant and form a barrier against conventional cleaning and disinfection procedures, making it more difficult to remove the biofilm. Biofilms easily develop in hydrated environments, with a source of carbon and nutrients such as nitrogen, phosphorus and potassium, among others.

DETECTION OF BIOFILMSBiofilms are invisible to the naked eye, but they can be detected.ITRAM HIGIENE has developed BioFinder, an innovative solution for the detec-tion of biofilms in open surfaces and an effective tool for hygiene monitoring.

BioFinder reacts in the presence of biofilmsYou can immediatelly spot any contaminated areas by a simple visual inspection just after spraying BioFinder on the facilities’ surfaces.

BioFinder offers a series of significant advantages over other detection methods.

· It’s a quick and affordable technique.· It simplifies monitoring processes in

surface hygiene.· It can treat large areas with its formula

and packaging types.· It doesn’t stain or leave residues on

surfaces due to its high water solubility which aids in rinsing.

· Thanks to its simple application and response type, handling by technical staff is not required.

1.Stainless steel surface moiste-

ned with water.

2.Negative reaction after using

BioFinder on a non contamina-ted surface.

3.Positive reaction after using

BioFinder on a surface contami-nated with biofilms.

3b.Side view of a positive reaction

after using BioFinder on a surface contaminated with

biofilms.

BioFinder has been tested on the most relevant microorganisms found in the food industry. From a microbiological standpoint of food safety: those capable of generating foodborne disease outbreaks such as Listeria monocytogenes, Salmonella spp., Escherichia coli, Staphylo-coccus aureus and Cronobacter sakazakii. From a technological standpoint: spoilage flora that can affect food product shelf life mainly as Pseudomonas spp.

Biofinder is effective in detecting monoespecies and multispecies biofilms, i.e. biofilms formed by only one type of microorganism, and those consisting of two or more species.

REMOVAL OF BIOFILMSEnzyme based products.ITRAM HIGIENE has developed an enzyme based range of products which currently are the most effective solution for the removal of biofilms, as they work specifically on EPS.

Itram HigieneC. Miramarges, 7, 1-4. 08500 Vic (Barcelona) - SPAINTel. +34 93 886 97 33. Fax +34 93 883 49 94E-mail: info@itramhigiene.com / www.itramhigiene.com

BIOFINDER TEST DEMOSTRATION

Esta información está basada en el estado actual de nuestros conocimientos y puede ser modificada sin previo aviso. Itram Higiene,

S.L. no se hace responsable del uso incorrecto del producto.

08500 Vic (Barcelona)

Tel.: +34 93 886 97 33

Fax: +34 93 883 49 94

info@itramhigiene.com

www.itramhigiene.com

www.biofilremove.com

Última actualización: 26-oct.-15

Página 1 de 2

DESCRIPTION

The Biofinder Demonstration Kit is a tool consisting of two agar plates of 90 mm, one red and one green.

This kit is used to demonstrate the positive (foaming) and negative reaction of Biofinder.

PHYSICOCHEMICAL PROPERTIES

COLOR pH COMPOSITION

Positive control Intense red 7,2 ± 0,2 Contains casein peptone, soy

peptone, sodium chloride,

bacteriological agar, yeast extract,

corn starch, sheep blood

Negative control Intense green 8,6 ± 0,2 Contains sucrose, sodium chloride,

sodium thiosulfate, sodium citrate,

meat peptone, casein peptone,

yeast extract, bile, sodium cholate,

ferric ammonium citrate, thymol

blue, bromothymol blue,

bacteriological agar

BIOFINDER TEST DEMOSTRATION

Esta información está basada en el estado actual de nuestros conocimientos y puede ser modificada sin previo aviso. Itram Higiene,

S.L. no se hace responsable del uso incorrecto del producto.

08500 Vic (Barcelona)

Tel.: +34 93 886 97 33

Fax: +34 93 883 49 94

info@itramhigiene.com

www.itramhigiene.com

www.biofilremove.com

Última actualización: 26-oct.-15

Página 2 de 2

HOW TO USE:

1. For optimum results, allow the plates to reach room temperature with a moist but not wet surface.

2. Open the plate and spray with a small amount of Biofinder.

3. Wait between 30 and 60 seconds.

4. On the red plates, a dense, white foam will appear (see picture below). This is the reaction you will

see when the presence of biofilm is detected. In contrast, on the green plates, will be no reaction.

This is what will be seen on a surface with no biofilms present.

STORAGE

The prepared medium should be stored under refrigeration, between 4 and 15°C and preferably in the

dark. Avoid excessive thermal shocks.

RECOMMENDATIONS

Use before expiration date. The expiration and the lot number are labelled underneath the plate.

PRESENTATION

Boxes of 40 units of positive plates.

Boxes of 40 units (20 units of positive control plates and 20 units of negative control plates).

Rev.: 0

Date: October 2015