welcome to the macroworld of microbiology 13 2

Welcome to the Macroworld of Microbiology

Second year Lab Medicine (Females)1433- 1434 A.H. / 2012- 2013

Microbiology Practical (Course I)

By:

Mrs. Hibah Abdul-Wahab I. Abu-Sulaiman

What are the life forms?

Humans.Animals.Plants.

– And…

Microbes

4 WH Questions

• What are microbes?

• Where do they live?

• When do they survive?

• Why they are participating other life forms?

So the study of microbes is called…

Microbiology

Why do we study microbiology??

Microbes have dominated Earth for more than 3 billion years.

Why do we study medical microbiology???

Nine out of ten cells in our bodies are microbes

So, why are we not suffering from any disease all the time?

When microbes can cause a disease?

Weak immune system.Pathogenic microbial invasion.Invasion to a sterile body site.

Objectives

Types of microbes.Definition of microbes.A sample story in bacteriology lab.

Microbes Include…

Bacteria.Viruses.Parasites.Fungi.

Bacteria

• Unicellular prokaryotes.

• Examples:– Staphylococcus spp.– Vibrio cholera.

Viruses

• Smallest infectious particle.

• Example:– Rotavirus.

Parasites

• Eukaryotes, unicellular or multicelluar.

• Example:– Giardia lambia.

Fungi

• Eukaryotes, in a unicellular or filamentous form.

• Example:– Trichophyton rubrum

An ending story…

Please…

Don’t sleep

Once upon a time…

Hinds’ 12th birthday

In the next day…

She went to A Gastroenterologist

A Stool Sample Was Requested

In Microbiology Lab

In the Next Day

And After Reading the Culture Media

And Performing the Biochemical Tests

It was Escherichia coli

The Microbiologist Decided to

Perform Antibiotic Susceptibility Testing (AST)

In the Next Day of AST

After 3 Days of Medication

Am I right when I said the Macroworld of Microbiology

When someone speaks about another,

What is the first thing that comes into your mind??!

Face

Is it…?

Rectangular face

Spherical face

Elongated face

Square shaped face

Triangular face

His Behavior

Does he like to work in a group, alone or…?

So now after knowing these two things, you can speak

to him/ her

They live and behave like we do in our society

Objectives

• To define different bacterial morphologies.

• To define their arrangements together.

Face

Is it…?

Rectangular faceSpherical face

Elongated face

Bacteria are exactly the same…

Spherical face

Elongated face

Rectangular face

What about their arrangements?!

How does the Turkish pilgrims look like in their groups?

Accordingly…

Staphylococci

Staphylococci Staff

How does the Malaysian pilgrims look like in their groups?

Accordingly…

Streptococci

Streptococci strip

In pairs

Diplococci

The same is applied for bacilli

Spirilla

Is there any other type?!

Vibrio

Some are flagellated

Hmm, did you like bacteria?!

Lets revise their morphologies and arrangements

Before entering

The Microbiology Lab

Be sure of following the safety rules

Safety in Microbiology Laboratory

Aims of following safety rules and practices

• To prevent the occurrence of accidental infections in the lab and reduce them if they occur.

• Prevent potentially harmful microbes from leaving the lab.

Safety in the Microbiology Lab

• Wash your hands with soap and water before you begin and after you finish your work and before leaving the lab.

• Decontaminate your work surface before and after you work

Safety in the Microbiology Lab

• No bags inside the lab.

Safety in the Microbiology Lab

• Wear only closed-toed shoes in the laboratory.

Safety in the Microbiology Lab

• Wear lab scrub and fully- buttoned lab coat.

Safety in the Microbiology Lab

Safety in the Microbiology Lab

• Tight your hair.• No cosmetics

• If you are:– Injured…– Immunocompromised..– Pregnant…– Allergic to latix…

Safety in the Microbiology Lab

• Be careful not to burn yourself when using Bunsen burner.

Safety in the Microbiology Lab

• Keep your hands away from your mouth and eyes…

Safety in the Microbiology Lab

• Do not eat, drink or chew gum while you are in the lab

Safety in the Microbiology Lab

• Avoid using electronic devices …– Mobiles, MP3 players,

laptops…

Safety in the Microbiology Lab

• Never mouth pipetting.

Safety in the Microbiology Lab

• Always keep cultures in a test tube rack whether you are working at your bench or walking about in the lab.

Safety in the Microbiology Lab

• Place all contaminated tubes, plates and waste materials in appropriate receptacles for subsequent sterilization.

Safety in the Microbiology Lab

• If you spill a chemical on your skin, immediately wash the affected area. If a chemical or a culture gets into your eye, immediately wash your eye for 5 min.

Safety in the Microbiology Lab

• If anything containing viable bacteria spills, cover the spell or broken glass with a paper towel and a generous amount of disinfectant to remain on the slip for at least 20 min. before cleaning it up.

Safety in the Microbiology Lab

• Sweep up pieces of broken glass (after disinfection if necessary) and place them in the specified container.

Safety in the Microbiology Lab

• Place sharps, such as syringes and slides, in a designated sharp containers.

Safety in the Microbiology Lab

• Do not keep cultures in your lab drawer or locker when you have finished with them.

• Do not remove cultures, reagents, or other materials from the lab unless you have permission to do so.

Safety in the Microbiology Lab

• Know the first aid kit location in your lab.

Safety in the Microbiology Lab

Recommended Book

Stay Tuned

To Contact Me

• Register on FB:

Hibah’s microclasses

Note: please send your name and Gp#

• e-mail: abusulaiman.hw1@gmail.com

Thank you for paying attention

Any Questions???