welcome to the macroworld of microbiology 13 2
DESCRIPTION
My classical and favorite welcome presentation for 2nd yr LM (females), UQU.TRANSCRIPT
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Welcome to the Macroworld of Microbiology
Second year Lab Medicine (Females)1433- 1434 A.H. / 2012- 2013
Microbiology Practical (Course I)
By:
Mrs. Hibah Abdul-Wahab I. Abu-Sulaiman
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What are the life forms?
Humans.Animals.Plants.
– And…
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Microbes
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4 WH Questions
• What are microbes?
• Where do they live?
• When do they survive?
• Why they are participating other life forms?
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So the study of microbes is called…
Microbiology
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Why do we study microbiology??
Microbes have dominated Earth for more than 3 billion years.
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Why do we study medical microbiology???
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Nine out of ten cells in our bodies are microbes
So, why are we not suffering from any disease all the time?
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When microbes can cause a disease?
Weak immune system.Pathogenic microbial invasion.Invasion to a sterile body site.
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Objectives
Types of microbes.Definition of microbes.A sample story in bacteriology lab.
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Microbes Include…
Bacteria.Viruses.Parasites.Fungi.
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Bacteria
• Unicellular prokaryotes.
• Examples:– Staphylococcus spp.– Vibrio cholera.
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Viruses
• Smallest infectious particle.
• Example:– Rotavirus.
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Parasites
• Eukaryotes, unicellular or multicelluar.
• Example:– Giardia lambia.
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Fungi
• Eukaryotes, in a unicellular or filamentous form.
• Example:– Trichophyton rubrum
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An ending story…
Please…
Don’t sleep
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Once upon a time…
Hinds’ 12th birthday
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In the next day…
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She went to A Gastroenterologist
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A Stool Sample Was Requested
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In Microbiology Lab
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In the Next Day
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And After Reading the Culture Media
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And Performing the Biochemical Tests
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It was Escherichia coli
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The Microbiologist Decided to
Perform Antibiotic Susceptibility Testing (AST)
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In the Next Day of AST
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After 3 Days of Medication
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Am I right when I said the Macroworld of Microbiology
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When someone speaks about another,
What is the first thing that comes into your mind??!
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Face
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Is it…?
Rectangular face
Spherical face
Elongated face
Square shaped face
Triangular face
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His Behavior
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Does he like to work in a group, alone or…?
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So now after knowing these two things, you can speak
to him/ her
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They live and behave like we do in our society
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Objectives
• To define different bacterial morphologies.
• To define their arrangements together.
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Face
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Is it…?
Rectangular faceSpherical face
Elongated face
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Bacteria are exactly the same…
Spherical face
Elongated face
Rectangular face
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What about their arrangements?!
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How does the Turkish pilgrims look like in their groups?
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Accordingly…
Staphylococci
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Staphylococci Staff
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How does the Malaysian pilgrims look like in their groups?
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Accordingly…
Streptococci
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Streptococci strip
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In pairs
Diplococci
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The same is applied for bacilli
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Spirilla
Is there any other type?!
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Vibrio
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Some are flagellated
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Hmm, did you like bacteria?!
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Lets revise their morphologies and arrangements
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Before entering
The Microbiology Lab
Be sure of following the safety rules
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Safety in Microbiology Laboratory
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Aims of following safety rules and practices
• To prevent the occurrence of accidental infections in the lab and reduce them if they occur.
• Prevent potentially harmful microbes from leaving the lab.
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Safety in the Microbiology Lab
• Wash your hands with soap and water before you begin and after you finish your work and before leaving the lab.
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• Decontaminate your work surface before and after you work
Safety in the Microbiology Lab
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• No bags inside the lab.
Safety in the Microbiology Lab
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• Wear only closed-toed shoes in the laboratory.
Safety in the Microbiology Lab
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• Wear lab scrub and fully- buttoned lab coat.
Safety in the Microbiology Lab
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Safety in the Microbiology Lab
• Tight your hair.• No cosmetics
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• If you are:– Injured…– Immunocompromised..– Pregnant…– Allergic to latix…
Safety in the Microbiology Lab
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• Be careful not to burn yourself when using Bunsen burner.
Safety in the Microbiology Lab
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• Keep your hands away from your mouth and eyes…
Safety in the Microbiology Lab
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• Do not eat, drink or chew gum while you are in the lab
Safety in the Microbiology Lab
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• Avoid using electronic devices …– Mobiles, MP3 players,
laptops…
Safety in the Microbiology Lab
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• Never mouth pipetting.
Safety in the Microbiology Lab
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• Always keep cultures in a test tube rack whether you are working at your bench or walking about in the lab.
Safety in the Microbiology Lab
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• Place all contaminated tubes, plates and waste materials in appropriate receptacles for subsequent sterilization.
Safety in the Microbiology Lab
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• If you spill a chemical on your skin, immediately wash the affected area. If a chemical or a culture gets into your eye, immediately wash your eye for 5 min.
Safety in the Microbiology Lab
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• If anything containing viable bacteria spills, cover the spell or broken glass with a paper towel and a generous amount of disinfectant to remain on the slip for at least 20 min. before cleaning it up.
Safety in the Microbiology Lab
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• Sweep up pieces of broken glass (after disinfection if necessary) and place them in the specified container.
Safety in the Microbiology Lab
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• Place sharps, such as syringes and slides, in a designated sharp containers.
Safety in the Microbiology Lab
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• Do not keep cultures in your lab drawer or locker when you have finished with them.
• Do not remove cultures, reagents, or other materials from the lab unless you have permission to do so.
Safety in the Microbiology Lab
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• Know the first aid kit location in your lab.
Safety in the Microbiology Lab
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Recommended Book
Stay Tuned
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To Contact Me
• Register on FB:
Hibah’s microclasses
Note: please send your name and Gp#
• e-mail: [email protected]
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Thank you for paying attention
Any Questions???