the principle of uvc disinfection

The principle of UVC disinfectionwith UVC254nm against all types of microorganisms

UV radiation is generally divided into the areas of UVA, UVB and UVC. UV radiation has a shorter wavelength and more energy than visible light. By the term UVC disinfection, it therefore does not bond the entire range of 100-300 nm, but particularly the range around 260 nm, where the DNA is most sensitive. There, the energy of the photons reacts with the DNA and destroys it.

Hygiene and sterilization with UVC is more important than ever in times of convenience food, MRSA in hospitals, globalization and industrial agriculture. Particularly where quality and safety are of great importance for humans, animals and products, this effi cient and powerful method is used. Why and how the UVC technology operates without residue around the clock and without the use of chemicals against all types of microorganisms, has been scientifi cally proven.

Thymine dimer formation in the DNA

Uracile dimer in the RNA

DNA and RNA are destroyed

Transcription and translation are stoppedNo DNA copy or protein biosynthesis

Cell division, proliferation and cell function are blocked

Microorganisms of all kinds die

An analysis of the disinfection level of UVC (green line) shows the highest effi ciency between 240-280 nm with a maximum at 260 nm. If you compare the absorption spectrum of the genetic material (DNA, blue line), you see an almost identical course.

The DNA molecule consists of four basic building blocks (adenine, thymine, guanine and cytosine) in a double helix. If two thymines are next to each other, the UVC acts by destroying the connection to the opposite strand and linking them to form stable dimers. UVpro tubes emit most of their photons at 254 nm (red bar), exactly at the maximum of DNA destruction.

The higher the UVC intensity [mW / cm²] and longer the irradiation time [s], the higher the UVC dose [mJ / cm²] and thus the number of lethal DNA damages for the microorganisms.

www.UVpro.de

orca GmbH · Hungenbach 1d · 51515 Kürten · Tel. +49 (0)2268/90830-0 · info@UVpro.de · www.UVpro.de

1µm

Plasmamembran

cell wall

flagellum

cyto plasm

DNA (RNA)

BAKTERIUM

cytoplasm nuclear Zellkern

chromatin (DNA)nucleus

plasma membrane1µm

YEAST

envelope

1µm

plasma membrane

„packed“ DNAcytoplasm

cell wall

SPORE

Bacteria and viruses

• DNA (RNA) barely protected

• small cells• simple cell without

nucleus

low LD90 dose

Yeasts and vegetativefungal cells

• DNA as chromosomes• big cell with organelles• complex cell with

nucleus

medium LD90 dose

Mold spores

• DNA packed in a complex way

• dense cell volume• robust cell wall with

pigments

high LD90 dose

DNA as chromosomesbig cell with organelles

dose

VirusesPRRS Virus Infl uenza A VirusHerpes Virus Hepatitis A VirusRota Virus SA11

BacteriaCampylobacter spec.Legionella spec.Escherichia coliSalmonella spec.Pseudomonas specStreptococcus spec.Staph. aureusListeria spec.Bacillus subtilis (Sp.)

YeastsSaccharomyces. ellip.Sacch. cerevisiaeSacch. carlsbergensis Candida albicans

Mold sporesPenicillium roquefortiiMucor mucedo Penicillium digitatumAspergillus glaucusAspergillus niger

1,82,14,36,77,5

2,22,32,54,34,54,54,85,06,8

3,56,27,511,0

1318384498

LD90-dose as a measure for disinfectionSize, structure and pigmentation infl uence the UVC eff ect

1-6 mJ/cm²

4-10 mJ/cm²

8-100 mJ/cm²

UVC disinfection with UVpro• Residue-free measures against germs in water, air and

on surfaces around the clock

• Easy installation, retrofi ttable into existing systems,variable use

We plan your project with a software-based 3D simulation and develop effi cient solutions tailored to your needs.

Up to 99,99% fewer germs

without UVC with UVC

In UVC disinfection, there is a specifi c lethal dose (LD) for each type of microorganism. Resistances do not exist! The standard is the LD90, the dose at which 90% of a population is killed.

*Exemplary values from the literature without guarantee and claim to completeness

Typical LD90 Doses* [mJ/cm²]