t thieme
Post on 26-Jan-2015
105 Views
Preview:
DESCRIPTION
TRANSCRIPT
Impact of Roundup Ready® maize production system on NTO’s
“North Europe”
NTO’s Thomas Thieme (BTL)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Application, weed Sabine Kappes (Biovativ GmbH)
Soil characterisation Andre Schlichting (STZ Soil Biotechnology)
Sponsored by Monsanto
Methods: Trial design
T2
T2
T2T3
T3
T3
T4
T4
T4
T5
T5
T5
T1
T1
T1
N�
Test site
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
T2
T2
T3
T3
T4
T4 T5
T5
T1
T1
27m
30m
Treatment 1: seed MONUMENTALHerb Metusolam+Flufenacet (A) A Pre-emergenceHerb Bromoxynil (C)Herb Iodosulfuron+Foramsulfuron (C) (2007-05-10, 2008-05-02)
Treatment 2: seed MONUMENTAL B V2-3Herb Metolachlor+Terbutylazine (B)Herb Mesotrione (B) (2007-05-23, 2008-05-27/29)Herb Rimsulfuron (C)
Treatment 3: seed RR CORN C V6-8
Methods: Treatment schedule in 2007 and 2008
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Treatment 3: seed RR CORN C V6-8Herb MON 78044 = Glyphosate (B)Herb MON 78044 = Glyphosate (C) (2007-06-11, 2008-06-17)
Treatment 4: seed RR CORNHerb MON 69447 = Acetochlor (B)Herb MON 78044 = Glyphosate (B)Herb MON 78044 = Glyphosate (C)
Treatment 5: seed RR CORNHerb Metolachlor + Terbotylazine (B)Herb Mesotrione (B)Herb Rimsulfuron (C)
Methods: Sampling with pitfall traps (PT)
4 PT/plot; sampling dates: 2007 May-Sep, 9; 2008 May-Sep, 9
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Methods: Sampling with yellow sticky traps (YST)
4 YST/plot; sampling dates: 2007 Jun-Oct, 8; 2008 May-Sep, 9
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Methods: Visual assessments (VA)
15/20 stems/plot in 2007/2008; assessment dates: 2007 Jun-Oct, 9; 2008 May-Sep, 9
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Number of NTO’s per treatment caught/observed
2007 2008* Sampling method T1 T2 T3 T4 T5 T2 T3 T4 T5
PT 6868 5984 6599 6693 5597 7911 8332 8458 8847
YST 2693 2735 2409 2635 2974 1589 1724 1572 2010
VA 436 467 520 492 500 1660 12997 5933 1463 ∑ 9997 9186 9528 9820 9071 11160 23053 15963 12320
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
* The very first application (A) at pre-emergence failed, therefore the arthropods
in plots of T1 are not taken into consideration
Total/year 47602 62496
Total 110098
Number of arthropod taxa in PT, YST and VA
Taxa in 2007 (n) Taxa in 2008 (n) Sampling method Identified
T2 Sig. differences
T4
Identified T2
Sig. differences T4
PT 132 11 124 5
YST 11 4 20 1
VA 11 3 17 4
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Taxa showing significant differences using ANOVA (AN) or Kruskal-Wallis (KW)
Herbivores (H) and predators (P) caught 2007 and 2008 in PT
Taxon Test Sample T1 T2 T5 T3 T4
2007
H & P Amara spp. KW 8 a ab abc c bc
KW 9 ac b c ab abc
H Harpalus spp. KW 3 ab a a b a
H Harpalus affinis KW 3 a a a b a
P Anchomenus dorsalis AN 4 a a a b ab
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
P Anchomenus dorsalis AN 4 a a a b ab
AN 5 ab ab b ab a
P Bembidion spp. KW 7 a b ab b a
P Calathus fuscipes KW 6 ac b ab c ab
P Clivina fossor KW 9 ab ac ac b c
P Pterostichus spp. AN 4 ab a ab ab b
KW 9 a a ab b a
P Pterostichus melanarius AN 4 ab a ab ab b
KW 9 a a ab b a
H & P Staphylinidae AN 5 ab ab ab a b
KW 7 a a a a b
H & P Aleocharinae AN 5 ab ab ab a b KW 7 a a a a b
Taxa showing significant differences using ANOVA (AN) or Kruskal-Wallis (KW)
Herbivores (H) and predators (P) caught 2007 and 2008 in PT
Taxon Test Sample T2 T5 T3 T4
2008
P Oedothorax spp. AN 2 a ab b ab
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
P AN 2 a ab b ab
P Oedothorax apicatus AN 2 a ab b ab
P Oedothorax retusus AN 5 a b ab ab
P Calathus spp. KW 1 a a a b
P Trechus quadristriatus KW 3 a a b a
Predators in PT: Araneae
2007 2008
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
30
40
50
60
70
80
App
l 2
Ap
pl 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
20
30
40
50 Ap
pl 1
Ap
pl 2
Ap
pl 3
T1
T2
T3
T4
T5
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
T1 T2 T5 T3 T4a a a a a
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap0
10
20
30
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
10
20
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
2007 2008
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
20
30
40
50
App
l 2
Ap
pl 3
Ap
pl 1 T1
T2
T3
T4
T5
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
15
20
25
30
35
Ap
pl 1
Ap
pl 2
Ap
pl 3
Predators in PT: Oedothorax apicatus (Araneae: Linyphiidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0
10
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
5
10
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
T2 T5 T3 T4a ab b ab
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
1.5
2.0
2.5
3.0
3.5T2
T3
T4
T5
App
l 2
Ap
pl 3
Ap
pl 1
2007 2008
Predators in PT: Oedothorax retusus (Araneae: Linyphiidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0.0
0.5
1.0
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
T2 T5 T3 T4a b ab ab
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
3
4
5
6
7
8
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
2
3
4
5
App
l 2
Ap
pl 3
Ap
pl 1 T1
T2
T3
T4
T5
Herbivores in PT: Amara spp. (Col.: Carabidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
1
2
3
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
0
1
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
T1 T2 T5 T3 T4a ab abc c bc
T1 T2 T5 T3 T4ac b c ab abc
10
15
20
25
App
l 2
Ap
pl 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
10
15
20
25
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Predators in PT: Bembidion spp. (Col.: Carabidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
0
5
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
5
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
T1 T2 T5 T3 T4a b ab b a
7.5
10.0
12.5
15.0
App
l 2
Ap
pl 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
4
6
8
10
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Predators/Herbivores in PT: Aleocharinae (Col.: Staphylinidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
0.0
2.5
5.0
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
Days after sowing0 10 20 30 40 50 60 70 80 90 100 110 120 130
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
2
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
T1 T2 T5 T3 T4ab ab ab a b
T1 T2 T5 T3 T4a a a a b
Taxa showing significant differences using ANOVA (AN) or Kruskal-Wallis (KW)
Herbivores (H) and predators (P) caught on YST
Taxon Test Sample T1 T2 T5 T3 T4
2007
H Miridae AN 5 a b b b b
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
H & P Syrphidae KW 4 a a a b ab
P Coccinellidae KW 2 a a b b a P Propylea
quatuordecimpunctata KW 2 a a b ab a
2008
H Auchenorrhyncha AN 2 a ab b ab
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
6
9
12
15
Ap
pl 2
App
l 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
30
40
50
60
70
80
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Herbivores in YST: Auchenorrhyncha (Hemiptera)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 20 40 60 80 100 120 140
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0
3
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
17
.09.
Days after sowing0 20 40 60 80 100 120 140 160
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
10
20
30
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
20.0
9.
30.0
9.
10.1
0.
T2 T5 T3 T4a ab b ab
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
1.0
1.5
2.0
2.5
3.0
Ap
pl 2
App
l 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
4
6
8
10
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Predators in YST: Propylea quatuordecimpunctata (Col.: Coccinellidae)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 20 40 60 80 100 120 140
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0.0
0.5
1.0
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
17
.09.
Days after sowing0 20 40 60 80 100 120 140 160
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
2
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
20.0
9.
30.0
9.
10.1
0.
T1 T2 T5 T3 T4a a b ab a
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
4
6
8
10
Ap
pl 2
App
l 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
6
9
12
15
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Predators in YST: Syrphidae (Diptera)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 20 40 60 80 100 120 140
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0
2
4
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
17
.09.
Days after sowing0 20 40 60 80 100 120 140 160
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0
3
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
20.0
9.
30.0
9.
10.1
0.
T1 T2 T5 T3 T4a a a b ab
Dipteranean families caught on colour traps during trials located in Germany and The Netherlands
(natural enemies of pests on crop analyzed;
not associated with crop, but attracted by colour)
Species
Episyrphus balteatus
Eupeodes corollae
Family Sum
… …
Do we have sufficient information if only the family is known?
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Helophilus affinis
Melanostoma mellinum
Sphaerophoria scripta
Syrphus torvus
Syrphus vitripennis
Trichopsomyia lucida
Eristalis abusiva
Brachyopa pilosa
…
Anthomyiidae
Cecidomyiidae
Chloropidae
Drosophilidae
Phoridae
Sciaridae
Syrphidae
…
…
6929
7424
7176
18395
7506
11342
6063
…
Taxon Test Sample T1 T2 T5 T3 T4
2007 H Miridae AN 3 a ab c bc bc P
Taxa showing significant differences using ANOVA (AN) or Kruskal-Wallis (KW)
Herbivores (H) and predators (P) observed in VA
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
P Anthocoridae KW 3 a a b b ab P Nabidae KW 2 a a a ab b 2008
H Aphididae KW 4 ac c b ab H Aphis frangulae KW 4 a a b b P Neuroptera KW 5 a b b b P Chrysopidae KW 5 a b b b P Anthocoridae AN 5 ab b a ab
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
150
200
250
300
350T2
T3
T4
T5
Ap
pl 2
App
l 3
Ap
pl 1
2007 2008
Herbivores in VA: Aphididae (Hemiptera: Sternorrhyncha)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 20 40 60 80 100 120 140
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap0
50
100
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
17
.09.
T2 T5 T3 T4ac c b ab
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0.4
0.6
0.8
1.0
Ap
pl 2
App
l 3
Ap
pl 1
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0.10
0.15
0.20
0.25
0.30
Ap
pl 1
Ap
pl 2
Ap
pl 3
2007 2008
T1
T2
T3
T4
T5
Predators in VA: Chrysopidae (Neuroptera)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Days after sowing0 20 40 60 80 100 120 140
Me
an
nu
mbe
r o
f a
rthro
pod
s p
er
trap
0.0
0.2
30
.04.
10
.05.
20
.05.
30
.05.
09
.06.
19
.06.
29
.06.
09
.07.
19
.07.
29
.07.
08
.08.
18
.08.
28
.08.
07
.09.
17
.09.
Days after sowing0 20 40 60 80 100 120 140 160
Mea
n n
um
ber
of a
rthro
po
ds p
er
trap
0.00
0.05
0.10
03.0
5.
13.0
5.
23.0
5.
02.0
6.
12.0
6.
22.0
6.
02.0
7.
12.0
7.
22.0
7.
01.0
8.
11.0
8.
21.0
8.
31.0
8.
10.0
9.
20.0
9.
30.0
9.
10.1
0.
T2 T5 T3 T4a b b b
Effect of weeds to the number of aphids observed during visual assessments
T3
T3
T4
T5
T5
Herbivores in VA: Aphididae (Hemiptera: Sternorrhyncha)
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
T3
T3
T4
Weed
Mean percentage coverage (± 95% confidence interval) for Total weed coverage
assessed along with and after application B in different treatments
Perc
enta
ge tota
l weed c
overa
ge 30.0
T2
20.0
T2T1
2007 2008
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
Perc
enta
ge tota
l weed c
overa
ge
Days after application B42190
20.0
10.0
0.0
-10.0
T2T3T4T5
Days after application B297
10.0
0.0
-10.0
T4T5
T3T2
The new method of controlling weeds using NK603 provided a delayed butsimilar level of control and had no negative indirect effects on NTO's when
tested under field conditions.
Conclusion
BTL
Bio-Test Labor GmbH Sagerheide Roundup Ready® Maize: The European Perspective, Brussels, March 22-24, 2010
top related