supplementary data cysteine multi-analytes selective dansyl … · 2019-04-01 · supplementary...

Supplementary Data

Multi-analytes selective dansyl derivative for the fluorescent detection of Cu(II) and

cysteine

Thangaraj Anand, Vinita Bhardwaj and Suban K Sahoo*

Department of Applied Chemistry, SV National Institute of Technology (SVNIT), Surat-395007,

Gujarat, India.

*Corresponding Author (Dr Sahoo): E-mail: suban_sahoo@rediffmail.com; Tel.: 91-261-

2201855.

Fig. S1. 1H NMR spectrum of DA in DMSO-d6.

Electronic Supplementary Material (ESI) for Photochemical & Photobiological Sciences.This journal is © The Royal Society of Chemistry and Owner Societies 2019

Fig. S2. 13C NMR spectrum of DA in DMSO-d6.

Fig. S3. HRMS spectrum of DA.

Fig. S4. ATR-FTIR spectrum of DA.

Fig. S5. UV-Visible absorbance spectra of DA (2.5×10-5 M) in DMSO:H2O (1:1, v/v) upon the

addition of Cu2+, Co2+, Ni2+, Mn2+, Mg2+, Fe3+, Fe2+, Zn2+, Cd2+, Hg2+, Pb2+, Al3+ and Cr3+ ions

(20 µL, 1×10-3M, H2O).

Fig. S6. (a) Benesi-Hildebrand expression fitting of fluorescence titration curve of DA upon

addition of Cu2+ ion.

Fig. S7. HRMS spectrum of DA with Cu2+ ion.

Fig. S8. DFT computed distribution of electron densities: HOMO’s and LUMO’s diagrams of

DA and its complex with Cu2+.

Fig. S9. HRMS Spectrum of DA-Cu2+ complex with Cys.

4000 3500 3000 2500 2000 1500 1000

DA+Cu2++Cys

%T

(a.u

.)

DA + Cu2+

Wavenumber, cm-1

Fig. S10. FT-IR spectra of the DA-Cu2+ and DA-Cu2+-Cys complexes.

Fig. S11. Benesi-Hildebrand expression fitting of fluorescence titration curve of DA-Cu2+ upon

addition of Cys.

Table S1. Comparison table of DA with some reported fluorescent sensor for Cu2+.

Systems Medium Analyte Detection limit Applications Ref*

Bodipy system CH3CN:H2O Cu2+ 0.87 µM Live cell imaging

1

Rhodamine-derived

Tris buffer Cu2+ 2 µM Live cell imaging

2

Pyrene-thiophene conjugate

Tris-HNO3 buffer Cu2+ 2×10-8M Real sample analysis

3

Julodine Schiff base

CH3CN:H2O Cu2+ 23.5 µM 4

Rhodamine Schiff base

CH3CN/Tris–HCl solution

Cu2+ 2.43×10-8 M 5

Rhodamine derivative

HEPES buffer Cu2+ 3 ppb bio imaging 6

NBD system HEPES buffer Cu2+ 0.84 µM Live cell imaging

7

Pyrazole system CH3CN:H2O Cu2+ 24 µM Live cell imaging

8

Azine based schif base

CH3CH2OH:buffer Cu2+ 3.4 × 10− 7 M Live cell imaging

9

Naphthol Derivative

DMSO:H2O Cu2+ 0.27 µM Live cell imaging

10

Thiazoline-Pyrene

DMSO:H2O Cu2+ 0.101mm Real sample analysis

11

Oligothiophene derivative

DMSO:H2O Cu2+ 4.2×10-7 M Real sample analysis

12

Naphthalene Schiff base

DMSO:H2O Cu2+ 7.25×10-8 M Test strip detection

13

Salicylaldehyde Schiff base

Tris buffer:DMSO Cu2+ 8.77 µM Real sample analysis

14

DA DMSO:H2O Cu2+ and Cys

0.979 µM and 1.76 µM

Real sample analysis

This work

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