southeastern grape improvement and distribution program …ncpngrapes.org/files/262325.pdf · 2017....

PRESENTED BY

Florida Agricultural and Mechanical University

PD Violeta Tsolova

Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences

Tallahassee, Florida.

Southeastern Grape Improvement and Distribution Program

Our Program The Southeastern NCPN Regional Clean Center for Grape is serving the viticulture region of Florida and neighboring Gulf States positioned in the PD’s (Pierce’s Disease) zone of the American Southeast. The mission of our Center is to provide high quality clonally-propagated grapevines regionally adapted muscadines, and PD’s tolerant American natives hybrids free of targeted transmittable, diseases through testing, diagnosis, and therapy.

Website: http://www.famu.edu/index.cfm?viticulture&FAMUNationalCleanPlant(NCPN)CenterforGrape

Florida A&M University’s Center for Viticulture and Small Fruit Research is maintaining for industry use the disease free G1/G2 nuclear planting stock of single copy origin grapevines and 4.5 acres Foundation Vineyard (G2) which currently is planted with 25 economically important muscadine and Florida native hybrid varieties.RESEARCH TEAMDr. Violeta Tsolova- Professor, Viticulture and Developmental Biology, Program Leader: (850) 412-7394; violeta.tsolova@famu.edu

Dr. Anthony. Ananga- Assistant Professor, Food Biotechnology: (850) 412-7393; anthony.ananga@famu.edu

Dr. Islam El-Sharkawy - Assistant Professot, Breeding and Crop Production, (850) 599-8685 islam.elsharkawy@famu.edu

Dr. James Obuya- Post Doctoral Research Associate, Plant Pathologist: (850) 412-5195; james.obuya@famu.edu

Our Program

Program Partners and Beneficiary

We work closely with the current programs of the NCPN, other academic institution in the region, local nurseries, Florida Viticulture Advisory Council, the Florida Wine and Grape Growers Association and the Florida Department of Agriculture through research, methods development, risk management, educational workshops and field days to advance the competitiveness and growth of the regional grape and wine industry and secure disease free planting stock for nurseries and individual growers.

Program ObjectivesProducing high quality disease free muscadine and Florida hybrid grape varieties by in vitro meristem culture.

Conduct research and developed rapid methods for diagnostic of muscadine and PD tolerant American native grape varieties for crown gall, viruses and Eutypa dieback.

Sustain the foundation block G1.

Operate the “Diagnostic Facility for American Native Southern Grapes”

Assist the extension and certification program.

Trends in FL Wine Industry

Growth of Certified Florida Wineries

1991- 3%1997-6%2003- 14%2010- 16%2014- 24%

www. FWGGA.orgwww. tryfloridawines.com

Industry Demand

Increase demand from nurseries in Florida, Georgia and Texas for licensing of our proprietary muscadinevariety “Majesty” and inclusion of new muscadinevarieties into the Southeastern Clean Vine Program due to an interest for exporting muscadineplanting stock abroad (China and Brazil).

Majesty

Patent US20100242143 - Muscadinegrape plant named 'Majesty

Grant/Onyx

FAMU Proprietary Selection

Floriana

FAMU Proprietary Selection

On going projects:New varieties (6) and breeding lines (3) are undergoing disease elimination and to be included in the “Clean Vine Foundation Block" by nursery request (partial NCPN funding FY 2015).

A rapid highly specific early monitoring program for Eutypa dieback in muscadine grapes using molecular diagnostics ( in state funding FY 2015)

Surveying GLRaV-1 in southern vineyards and in house diagnostic capability (in state funding FY 2016 )

To continue to evaluate the healthy status of the 1st generation vines in the Foundation vineyard in harmonization with the accepted guidelines of the Grape NCPN (1st audit completed in 2015 with partial NCPN FY2015 funding).

Two additional multi[purpose labs are under development in the newly designed "Viticulture Field/Head House Facilities Project: Postharvest Evaluation and Plant Pathology (Evans –Allen Formula Grant Funding).

Deaback in Muscadine Grapes

Reasons are complex and poorly understood:

Several factors (Rubio and Garzón, 2011) could be involved in recent trunk diseases

development:

i) Increased number of the contaminated asymptomatic stocks in vineyards.

ii) Infections brought to vineyards by infected planting material. Grapevine Trunk Diseases.

iii) Cultural practices in vineyards. These usually focused to have grape yield during the first years and, giving a poor pruning wound protection.

OIV Review: International Council on Grapevine Trunk Diseases (ICGTD)April 2016

Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines

‘Magnolia’ variety with GLRaV-1 symptoms

Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines

Observation and Scouting for Local Vectors

Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines

2015 Audit of the Foundation Vineyard

Viruses:

GLRAV-1muscadine samples only Grapevine leaf roll

associated virus-1

RBLenoir and Black Spanish cvs foundation material)

Grapevine red blotch associated virus

Source BlockBMP vineyard CV&SFR

(‘mother vines’ after 4 years quality observations

Disease Elimination:In vitro 0.3-05. mm meristem culture

25 varieties:19 muscadines+2 rootstocks

+ 6 FL hybrid var.

Pathogen Testing:18 viruses+Eytypa, Agro, PD

Pathogen Testing: after 6-12 months growth

21 viruses+Eytypa, Agro, PD

Disease Free G1 Nuclear Planting Stock -1 copy in screen house

Foundation vineyard

Certified Block in Registered NurseriesCertified Planting Stock for the Industry

Southeastern Vine Improvement and Distribution Program

Southeast Grape Certification Program

In state support

FL Ag Commissioner Adam Putnam touring our Center

In service of the Industry and Community

Viticulture Center Helps Invigorate Muscadine Wine Industry http://sustainability.famu.edu/latest/news/item/41-vit-center-invigorates-muscadinewine-industry

FAMU Hosts Grape Harvest Festivalhttp://www.famunews.com/2016/08/famu-hosts-grape-harvest-festival/

Sweet Florida Native Helps FAMU Enhance its Brandhttp://www.tallahassee.com/story/life/food/2016/02/24/sweet-florida-native-helps-famu-enhance-brand/80874308/

FAMU's Center for Viticulture and Small Fruit Research ...www.youtube.com/watch?v=y-yUUTtpjKoEnhancing and promoting research productivity - grantsmanship, publication, and participation in

professional organizations.

“At FAMU, Great Things Are Happening Every Day.”

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