scientific talks in general and masterpiece theatre in particular

Scientific Talks

in general and

Masterpiece Theatre

in particular.

The Ten Minute Rule

Distill your data down to one point

Then state the significance in one sentence

Step 1 :

Isolate the Take Home Message (THM)

Tarun Kapoor Examining mechanisms of cell division using real-time microscopy and chemical genetics

Mike Cox Genome restoration after radiation damage in Deinococcus radiodurans

Fall 2004 Speakers

Step 2 The Introduction

Purpose- Start from nothing and get to the THM

Remember:

Who’s your Daddy?

Just the Facts

•Determine the “main question” or hypothesis

•Very Specific

•The answer will have broad implications and be the THM

Remember:

Who’s your Daddy?

Just the Facts

Introduction

(The question)

Conclusion

Results

(The data and interpretation)

(The THM )

Q. How much data?

Q. How many slides?

A. = T/2(rule of thumb)

The main question dictates the data you present

Data orSlide orOverhead

?YES

NO

Who’s your Daddy?

Results Section

•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)

Conclusion:

Mutations in abc1 cause abnormal eye development

THM:

Eye development is regulated by ubiquitylation

Know Your Data

•Practice without it

•What the data are•What the data mean•Where the data lead

Step 3 The Conclusion

•Recap the question

•Recap the most important conclusion(s)

•State the THM

The Three Tellums

1. Tellum what you’re gonna tellum

2. Then tellum

3. Then tellum what you toldum

Slides - audiovisual aids

Tor signaling

•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases

YEPD YEPD + Ben

Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2

A

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

B

C WCE IgG IP Sup-IgG IP

Mad3-PrA

Mad2

Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +

1 2 3 4 5 6 7 8 9

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

D

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

YEPD YEPD + Ben

Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2

A

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

B

C WCE IgG IP Sup-IgG IP

Mad3-PrA

Mad2

Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +

1 2 3 4 5 6 7 8 9

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

D

Immunodeplete Mad3-PrA (IgG Sepharose)

(IgG IP)

WCE

SUP(IgG IP)

Immunodeplete Cdc20-13Myc (anti-Myc beads)

(Myc IP) SUP(Myc IP)

Pull down Mad3-PrA

(Blot Mad2)

WCE

SUP

Pulldown Cdc20-13Myc

Mad3

PrA

Mad2 Mad2

Cdc20

Myc

(Blot Mad2)

Sequential Immunoprecipitation

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Mad3

PrA

Mad2 Mad2

Cdc20

Myc

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Mad2

Cdc20

Myc

Mad3

PrA

Mad2

Sup-IgGIP IgG IP Myc IP Sup- Myc IP

Mad2

Cdc20-13Myc

Mad3-PrA - + + - + + - + + - +

Cdc20-13Myc + - + + - + + - + + +

1 2 3 4 5 6 7 8 9 10 11

Mad2

Cdc20

Myc

Mad3

PrA

Mad2

“Every product of genius is a product of enthusiasm”

Benjamin Disreali

The Delivery

And finally…………..

Dress for Success

The Ten Minute Rule

No matter what the talk/seminar is:

T/2 slides

Be professional