scientific talks in general and masterpiece theatre in particular
TRANSCRIPT
Scientific Talks
in general and
Masterpiece Theatre
in particular.
The Ten Minute Rule
Distill your data down to one point
Then state the significance in one sentence
Step 1 :
Isolate the Take Home Message (THM)
Tarun Kapoor Examining mechanisms of cell division using real-time microscopy and chemical genetics
Mike Cox Genome restoration after radiation damage in Deinococcus radiodurans
Fall 2004 Speakers
Step 2 The Introduction
Purpose- Start from nothing and get to the THM
Remember:
Who’s your Daddy?
Just the Facts
•Determine the “main question” or hypothesis
•Very Specific
•The answer will have broad implications and be the THM
Remember:
Who’s your Daddy?
Just the Facts
Introduction
(The question)
Conclusion
Results
(The data and interpretation)
(The THM )
Q. How much data?
Q. How many slides?
A. = T/2(rule of thumb)
The main question dictates the data you present
Data orSlide orOverhead
?YES
NO
Who’s your Daddy?
Results Section
•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)
Conclusion:
Mutations in abc1 cause abnormal eye development
THM:
Eye development is regulated by ubiquitylation
Know Your Data
•Practice without it
•What the data are•What the data mean•Where the data lead
Step 3 The Conclusion
•Recap the question
•Recap the most important conclusion(s)
•State the THM
The Three Tellums
1. Tellum what you’re gonna tellum
2. Then tellum
3. Then tellum what you toldum
Slides - audiovisual aids
Tor signaling
•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases
YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Pull down Mad3-PrA
(Blot Mad2)
WCE
SUP
Pulldown Cdc20-13Myc
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
(Blot Mad2)
Sequential Immunoprecipitation
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
“Every product of genius is a product of enthusiasm”
Benjamin Disreali
The Delivery
And finally…………..
Dress for Success
The Ten Minute Rule
No matter what the talk/seminar is:
T/2 slides
Be professional