recombinant dna, probes and techniques

Cindy Vargas Forero Third SemesterMedicine – UPB2010

Recombinant DNA, involves creating

VOLUNTARILY DNA molecules

consisting of DNA fragments coming

from different sources

probes are short fragments of

nucleic acids are used for

identification of gens

Also improved their ability to detect

smaller concentrations of reactive oxygen

species.

Replacing a hydrogen atom with

a deuterium

Increased the stability and shelf life of the dyes,

Deuterium is an isotope of hydrogen

that has a single proton and single

neutron in its nucleus.

Its atomic weight is therefore twice that of the much more common hydrogen atom, which lacks a

neutron.

When they designed and synthesized various

fluorescent probes with deuterium instead of

hydrogen

the dyes were not as susceptible to

spontaneous degradation by air and light as their hydrogen counterparts

which made them significantly more accurate

at detecting reactive oxygen species in cells and

animals.

It is important to understan that reactive oxygen

species are the produc of cellular stress and reacts

better on probes that have change a hydrogen in

fluoresent dyes

Researchers from MBIC are turning up the brightness on a group of

fluorescent probes called fluoromodules.

Fluoromodules that are used

to monitor biological

activities of individual proteins in real-time.

This latest advance enhances their fluormodule technology by causing it to glow an order of magnitude brighter than typical fluorescent proteins. The new fluoromodules are five- to seven-times brighter than enhanced green

fluorescent protein (EGFP)

This flouromodules makes brighter

proteis, because it amplified the signal, so it makes possible to follow an specific protein in real time.

Fluorescent probes are essential to studying a variety of inflammatory

diseases, including cancer and

atherosclerosis.

The probes detec and measure reactive

oxygen species, which play an important role in disease processes

The probes detect and measure reactive oxygen species, which play an important role in disease processes.The probes detect and measure reactive oxygen species, which play an important role in disease processes.The probes detect and measure reactive oxygen species, which play an important role in disease processes.

Fluoro modules, can be used to monitor biological activities of

individual proteins in living cells in real time.

nervous system diseases such as

Parkinson's, autism, Alzheimer.

the expression of genes and proteins in people with

mutations, evaluate specific proteins of different types of

cancer

MARTINEZ, S. Lina Maria. Biologia Molecular. Quinta edición. Medellin. UPB. Fac de Medicina, 2009.

“Replacing Hydrogen in Fluorescent Dyes Improves Detection Ability, Stability and Shelf Life”. Science Daily. July 20, 2010

“Brightness on Fluorescent Probes Used to Monitor Biological Activities of Individual Proteins Increased”. Science Daily. Aug. 19, 2010