8/19/2019 Recombinant Dna Vaccines

1/16

RECOMBINANT

DNAVACCINES

8/19/2019 Recombinant Dna Vaccines

2/16

VACCINE

  - a substance that is usuallyinjected into a person or anialto protect a!ainst a particulardisease

" a preparation o# $illedicroor!aniss% that isadinistered to produceiunity to a particular disease

Accordin! to the Merria & 'ebsterDictionary

8/19/2019 Recombinant Dna Vaccines

3/16

-is a techni(ue #orprotectin! an or!anis

a!ainst disease byinjectin! it )ith

!enetically en!ineeredDNA to produceiunolo!ical response

8/19/2019 Recombinant Dna Vaccines

4/16

*istory

+,-. & the start of

experiments outliningthe transfer of DNAinto cells of livinganimals

+,,/ &  Tang and Johnson reported therst successful growthhormone in mice

+,,0 & developmentof the 1st humanantibodies

8/19/2019 Recombinant Dna Vaccines

5/16

Construction

Copose o#bacterial

plasid

8/19/2019 Recombinant Dna Vaccines

6/16

8/19/2019 Recombinant Dna Vaccines

7/16

8/19/2019 Recombinant Dna Vaccines

8/16

8/19/2019 Recombinant Dna Vaccines

9/16

8/19/2019 Recombinant Dna Vaccines

10/16

8/19/2019 Recombinant Dna Vaccines

11/16

Types o# Vaccines1i2e% AttenuatedVaccines

 These vaccines are good“teachers of the immunes!stem" The! elicit strong

cellular and antibod! responsesand often confer to lifelongimmunit! with onl! one to two

doses

8/19/2019 Recombinant Dna Vaccines

12/16

Types o# Vaccines1i2e% AttenuatedVaccines-

#iruses undergo growinggeneration"- #accines against measles$

mumps and chic%enpox aremade b! this method

8/19/2019 Recombinant Dna Vaccines

13/16

Types o# VaccinesInacti2ated Vaccines- &cientists produce

inactivated vaccines b!%illing the disease causingmicrobe with chemicals$

heat or radiation"- &uch vaccines are moresafer and stable than live

vaccines

8/19/2019 Recombinant Dna Vaccines

14/16

Types o# VaccinesTo3oid Vaccines- #accines for those bacteria-

causing diseases thatsecretes toxin or harmfulchemicals

-

 Ta%en from the host andin'ected to the immunes!stem for ad'ustment and

stabilit!-

8/19/2019 Recombinant Dna Vaccines

15/16

Types o# VaccinesConju!ate Vaccines- #accines that are ta%en from

bacterium with outer coatingof sugar molecules calledpol!saccharides

-

#accine against in(uen)at!pe * is an example

8/19/2019 Recombinant Dna Vaccines

16/16

Types o# VaccinesDNA Vaccines- +enes from a microbe is

anal!sed and will be inducedas a vaccine and be in'ectedto the host so that the bod!

will create its antigenmolecules as a response