prosessing non foodmaterial into food...mannan marint protein nettverk - bjørge westereng, 5/11...

Norwegian University of Life SciencesMarint Protein Nettverk - Bjørge Westereng, 5/11 2020 1

Prosessing non food material into food

Bioraffinering ved NMBU

Campus Aas makes major efforts in results-based food research in line withWhite paper. 9 (2011-2012) – “Velkommen til bords”!

White paper. 7 (2014–2015), «Langtidsplan for forskning og høyere utdanning 2015–2024»

FoodPilotPlant Norway meets tomorrow’s needs for excellent research, education and

innovative solutionsKick off meeting 24/11

Phase 2: 2018 New application for further upgrading, renewal and optimization, 50 MNOK.

Slaughter wasteLignocellulose

Hydrolysate Yeast in solution(Single cell protein)

Dry Yeast

Ingredient in feed

Enzymatichydrolysis Fermentation Spraydrying

Enzymes Yeast

BiochemicalsFrom carbohydratesand lignin

Isolation and refining of biomolecules; WoodPrebiotics

pretreatment Enzymatic hydrolysis

Enzyme Production

Ultra/nanoFiltration

Spraydrying

Produksjon av nye typer gjær

Production and characterization of yeasts grown on media composed of spruce-derived sugars and protein hydrolysates from chicken by-products. Lapena et al. Microbial Cell Factories (2020)

Carbohydrates in disguise

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Main objective: “How to prepare wood caramels, Even healthy ones?” Wood Prebiotics

Woody biomass

ProducingCarbohydrates

PrebioticsSelectively promote growth of good gut microbes in animals and humans

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020 Steam explosion rig

Wood prebiotics koncept

Spruce caramel

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Xylan

Mannan

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Ester boundAcetic acid

Contains all featuresOf ‘food mannans’, 20% of the tree

Norwegian University of Life Sciences 10

Wood Prebioticsmicrobes

El Kaoutari et al. Nature Reviews, 2013

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Lignocellulose

Ingredient in feed

BiochemicalsFrom carbohydratesand lignin

Isolation and refining of biomolecules; WoodPrebiotics

pretreatment Enzymatic hydrolysis

Ultra/nanoFiltration

Spraydrying/Vacuum evaporation

Variousfiltrations

Choice ofmembraneessential

OtherdryingtechniquesSeverity and

pH matters

DP essentialGlucoseequivalents

SubstratesVery different

Filtration

Product mayaffect pelletdurability

Depth-, Bag-filtering

Pretreatment Ingredient production Feed production

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Bacteria and their enzymes, our best friends

Gran-karameller

Leanti La Rosa, Nature Communications, 2019

MicrobiologyMolecular biology, biochemistry

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Esterases

Michalak et al. PNAS, 2020

Multi-omics

Proteomics

MetaGenomicsFeeding trials

Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020 Michalak et al. Nature Communications (2020) in press

Secondary fermentation in beer

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Sour beer: long production time poor process control

Mannan and xylan to aid secondary fermentation. Sour beer fermenters simpler degradation systems. Need help (enzyme assisted substrate utilization) (PhD, Anna Dysvik)

https://radio.nrk.no/podkast/studio_2/nrkno-poddkast-25559-148146-21012019171400

https://forskning.no/menneskekroppen-bakterier-nmbu-norges-miljo-og-biovitenskapelige-universitet/harmoniske-tarmbakterier-sorger-for-effektiv-fordoyelse/271166

Media

Fermentation technology

https://pubs.acs.org/doi/pdf/10.1021/acs.est.8b00216

Area efficientProd. = 1600 cows/week

Seaweed Applications 17

The NMBU biorefineryApplied enzymologyFermentationBiogasMicrobial communitiesBiorefining/fractionationSteam explosion pretreatmentBiorefinery pilot plantAnalytics

Essential facilities – an advanced Analytical platform

System: Bruker UltrafleXtremeDetection: MS

MS modes: MS and MS/MS(PSD fragmentation)

Systems:• Dionex Ultimate 3000• Thermo Iontrap Velos Pro

Detection: UV, CAD and MS

MS modes: MS and MS/MS(CID and HCD fragmentation)

Discovery LC-MS

Column types:• Reversed-Phase C18• Porous Graphitic Carbon (PGC)• HILIC

Components:• Oligosaccharides (various forms)• VFAs

Components:• Di- and oligosaccharides• Peptides• Proteins

MALDI-MS

NMBU: KBM (Vincent Eijsink and Svein J.Horn, pictures+ Åsmund Røhr, Knut Rudi, Siv Skeie), Faculty ofBiosciences, Animal and aquaculturesciencesMargareth Øverland, Leidy Lagos, Ricardo Tavares Benicio, Jon Øvrum HansenNofimaSvein Knutsen, Simon BallanceNTNUFinn L. Aachmann

CAMBIPaal Jahre NielsenUniversity of Michigan Medical SchoolEric Martens, Nicolas Pudlo,Robert Glowacki, Gabriel Pereira

DTUMaher A. Hachem, Maria Louise LethMorten Ejby, Christopher Workman

University of ReadingGlenn Gibson,

Sustainable, a word with many facets

All contains considerableportions of exoticingredients. Replacingfat→long list ofsubstituting ingredients.

Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020 Tropical/exotic Nordic/local

Mayo

Light mayonaise, approx. 4% stabilizers ie. Polysaccharides.

Skogn

Industrial potensial: Hemicellulose production from Skogn could serve the entire pig-population in Norway, with a 4% inclusion level in the feed.

Marint Protein Nettverk - Bjørge Westereng, 5/11 2020

Other equipment to be installed during 2016

Steam explosion

Prefiltering- cell disrupter- High drymatterPumping equipment

modules «Off-site» Analytical platformState of the art/experienced users at the institute is instrumental

SFE-Supercritical-fluid Extraction system

4 Bench topfermentors

➢Many projects; FME, Foods of Norway, WoodPrebiotics, BioFeed, NorZymeD and BioMim (enzyme production)

Mass Spectrometry

NMR

Norwegian University of Life Sciences23

Carbohydrate esterases, structural biology/biochemistry• Carbohydrate esterases catalyze the de-O or de-N-acylation of substituted saccharides• Migration

Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020

Lab.

Ben

ch25

9*60

cm

Fum

eho

od17

9*80

cm

2/3 phaseSeparator190*80

Spray dryer190*190 cm

Sink110*80 cm

EmergencyEye washand shower

Sca

ffold

185*

75 c

m

Reo

l 3x4

box

es60

x80

195*

80 c

m

PalletStacker160*70 cmGafler 115 cm

FermentorTechfors S30 L80*80 cm

CIPTech-CIP108*75 cm

Microfluidizer100*90 cm

NF/U

FFiltration100*60 cm

Meat

grinder

Meat

grinder

HydrolysisTank200 l162*154 cm

Hydrolysis

Tank x230 l

HydroPressØ=60 cm

LenticFiltrationØ=60 cm

Mob

ile p

umps

110*

45 c

m

Electrisity, water, compressed air

Electrisity, compressed air

Vent.Canal

Vent.Canal

Vent.Canal

Vent.Canal

Vent.Canal

Wall support Crane

Wall support Crane

Pump

EL

EL

Wall support Crane

Ballast Tank 400Lit 100x100 cm

Heat exchanger3000x50x1300 LxBxH mm

Vac

uum

evap

orat

or16

0x80

cm

W x D x H = 1100 x 790 x 1800 mm

Twin screwMove solids

FermentorFermentor150 L110*75 cm

HomogenizerInbetween

meat grinder and

separatorxLxB cm

Steam