prosessing non foodmaterial into food...mannan marint protein nettverk - bjørge westereng, 5/11...
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Norwegian University of Life SciencesMarint Protein Nettverk - Bjørge Westereng, 5/11 2020 1
Prosessing non food material into food
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Bioraffinering ved NMBU
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Campus Aas makes major efforts in results-based food research in line withWhite paper. 9 (2011-2012) – “Velkommen til bords”!
White paper. 7 (2014–2015), «Langtidsplan for forskning og høyere utdanning 2015–2024»
FoodPilotPlant Norway meets tomorrow’s needs for excellent research, education and
innovative solutionsKick off meeting 24/11
Phase 2: 2018 New application for further upgrading, renewal and optimization, 50 MNOK.
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Slaughter wasteLignocellulose
Hydrolysate Yeast in solution(Single cell protein)
Dry Yeast
Ingredient in feed
Enzymatichydrolysis Fermentation Spraydrying
Enzymes Yeast
BiochemicalsFrom carbohydratesand lignin
Isolation and refining of biomolecules; WoodPrebiotics
pretreatment Enzymatic hydrolysis
Enzyme Production
Ultra/nanoFiltration
Spraydrying
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Produksjon av nye typer gjær
Production and characterization of yeasts grown on media composed of spruce-derived sugars and protein hydrolysates from chicken by-products. Lapena et al. Microbial Cell Factories (2020)
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Carbohydrates in disguise
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
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Main objective: “How to prepare wood caramels, Even healthy ones?” Wood Prebiotics
Woody biomass
ProducingCarbohydrates
PrebioticsSelectively promote growth of good gut microbes in animals and humans
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020 Steam explosion rig
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Wood prebiotics koncept
Spruce caramel
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
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Xylan
Mannan
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
Ester boundAcetic acid
Contains all featuresOf ‘food mannans’, 20% of the tree
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Norwegian University of Life Sciences 10
Wood Prebioticsmicrobes
El Kaoutari et al. Nature Reviews, 2013
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Lignocellulose
Ingredient in feed
BiochemicalsFrom carbohydratesand lignin
Isolation and refining of biomolecules; WoodPrebiotics
pretreatment Enzymatic hydrolysis
Ultra/nanoFiltration
Spraydrying/Vacuum evaporation
Variousfiltrations
Choice ofmembraneessential
OtherdryingtechniquesSeverity and
pH matters
DP essentialGlucoseequivalents
SubstratesVery different
Filtration
Product mayaffect pelletdurability
Depth-, Bag-filtering
Pretreatment Ingredient production Feed production
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
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Bacteria and their enzymes, our best friends
Gran-karameller
Leanti La Rosa, Nature Communications, 2019
MicrobiologyMolecular biology, biochemistry
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
Esterases
Michalak et al. PNAS, 2020
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Multi-omics
Proteomics
MetaGenomicsFeeding trials
Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020 Michalak et al. Nature Communications (2020) in press
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Secondary fermentation in beer
Marint Protein Nettverk - Bjørge Westereng, 5/11 2020
Sour beer: long production time poor process control
Mannan and xylan to aid secondary fermentation. Sour beer fermenters simpler degradation systems. Need help (enzyme assisted substrate utilization) (PhD, Anna Dysvik)
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https://radio.nrk.no/podkast/studio_2/nrkno-poddkast-25559-148146-21012019171400
https://forskning.no/menneskekroppen-bakterier-nmbu-norges-miljo-og-biovitenskapelige-universitet/harmoniske-tarmbakterier-sorger-for-effektiv-fordoyelse/271166
Media
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Fermentation technology
https://pubs.acs.org/doi/pdf/10.1021/acs.est.8b00216
Area efficientProd. = 1600 cows/week
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Seaweed Applications 17
The NMBU biorefineryApplied enzymologyFermentationBiogasMicrobial communitiesBiorefining/fractionationSteam explosion pretreatmentBiorefinery pilot plantAnalytics
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Essential facilities – an advanced Analytical platform
System: Bruker UltrafleXtremeDetection: MS
MS modes: MS and MS/MS(PSD fragmentation)
Systems:• Dionex Ultimate 3000• Thermo Iontrap Velos Pro
Detection: UV, CAD and MS
MS modes: MS and MS/MS(CID and HCD fragmentation)
Discovery LC-MS
Column types:• Reversed-Phase C18• Porous Graphitic Carbon (PGC)• HILIC
Components:• Oligosaccharides (various forms)• VFAs
Components:• Di- and oligosaccharides• Peptides• Proteins
MALDI-MS
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NMBU: KBM (Vincent Eijsink and Svein J.Horn, pictures+ Åsmund Røhr, Knut Rudi, Siv Skeie), Faculty ofBiosciences, Animal and aquaculturesciencesMargareth Øverland, Leidy Lagos, Ricardo Tavares Benicio, Jon Øvrum HansenNofimaSvein Knutsen, Simon BallanceNTNUFinn L. Aachmann
CAMBIPaal Jahre NielsenUniversity of Michigan Medical SchoolEric Martens, Nicolas Pudlo,Robert Glowacki, Gabriel Pereira
DTUMaher A. Hachem, Maria Louise LethMorten Ejby, Christopher Workman
University of ReadingGlenn Gibson,
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Sustainable, a word with many facets
All contains considerableportions of exoticingredients. Replacingfat→long list ofsubstituting ingredients.
Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020 Tropical/exotic Nordic/local
Mayo
Light mayonaise, approx. 4% stabilizers ie. Polysaccharides.
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Skogn
Industrial potensial: Hemicellulose production from Skogn could serve the entire pig-population in Norway, with a 4% inclusion level in the feed.
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Other equipment to be installed during 2016
Steam explosion
Prefiltering- cell disrupter- High drymatterPumping equipment
modules «Off-site» Analytical platformState of the art/experienced users at the institute is instrumental
SFE-Supercritical-fluid Extraction system
4 Bench topfermentors
➢Many projects; FME, Foods of Norway, WoodPrebiotics, BioFeed, NorZymeD and BioMim (enzyme production)
Mass Spectrometry
NMR
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Norwegian University of Life Sciences23
Carbohydrate esterases, structural biology/biochemistry• Carbohydrate esterases catalyze the de-O or de-N-acylation of substituted saccharides• Migration
Bærekraftig entreprenørskap - fremtidens mat- Bjørge Westereng, 8/1 2020
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Lab.
Ben
ch25
9*60
cm
Fum
eho
od17
9*80
cm
2/3 phaseSeparator190*80
Spray dryer190*190 cm
Sink110*80 cm
EmergencyEye washand shower
Sca
ffold
185*
75 c
m
Reo
l 3x4
box
es60
x80
195*
80 c
m
PalletStacker160*70 cmGafler 115 cm
FermentorTechfors S30 L80*80 cm
CIPTech-CIP108*75 cm
Microfluidizer100*90 cm
NF/U
FFiltration100*60 cm
Meat
grinder
Meat
grinder
HydrolysisTank200 l162*154 cm
Hydrolysis
Tank x230 l
HydroPressØ=60 cm
LenticFiltrationØ=60 cm
Mob
ile p
umps
110*
45 c
m
Electrisity, water, compressed air
Electrisity, compressed air
Vent.Canal
Vent.Canal
Vent.Canal
Vent.Canal
Vent.Canal
Wall support Crane
Wall support Crane
Pump
EL
EL
Wall support Crane
Ballast Tank 400Lit 100x100 cm
Heat exchanger3000x50x1300 LxBxH mm
Vac
uum
evap
orat
or16
0x80
cm
W x D x H = 1100 x 790 x 1800 mm
Twin screwMove solids
FermentorFermentor150 L110*75 cm
HomogenizerInbetween
meat grinder and
separatorxLxB cm
Steam