production of transgenic farm animals

PRODUCTION OF

TRANSGENIC FARM

ANIMALS

Prepared by: MUHSIN ALI AHMED

M.Sc. in Biotechnology

from Bangalore University-India

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CONTENTS:

Introduction

Extraction of Desire Gene

Methods of Transgenic Animal

Application of Animal Transgenic

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INTRODUCTION

Foreign DNA(gene) is introduced into the animal,

using recombinant DNA technology.

Deoxyribonucleic acid (DNA) is unit of genetic

which is responsible of all quality and quantity

animal characters.

DNA coiled and coiled with histone protein to

produce chromosome .

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EXTRACTION DESIRE GENE

For extraction of specific gene from of millions of

gene we need to Polymerase Chain Reaction

(PCR) technique and include:

DNA extraction

Thermo cycle device

Gene primer

Some chemical materials

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METHODS OF TRANSGENIC ANIMAL

1- Retroviral Vector Method

2- Sperm Mediated Gene Transfer

3- Microinjection Method

4- Embryonic Stem Cell

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1- RETROVIRAL VECTOR METHOD

when the virus vectors infect a host cell, the viral RNA is reverse transcribed in the cytoplasm making linear double-stranded DNA.

A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA.

In this method, retroviruses are used as vectors to transfer genetic material into the host cell

Infecting mouse embryo with a retrovirus which carry the new gene. 6

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2- SPERM MEDIATED GENE TRANSFER

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3- MICROINJECTION METHOD

1) transfer of a desired gene construct (of a single

gene or a combination of genes that are

recombined and then cloned) from another member

of the same species or from a different species into

the pronucleus of a reproductive cell;

2) in vitro culture of the manipulated cells to

develop to a specific embryonic phase; and

3) then transfer of the embryonic cells to the

recipient female.9

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4- EMBRYONIC STEM CELL

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APPLICATION OF ANIMAL TRANSGENIC

1- Agriculture like improve Biomass

2- Disease Resistance like avian influenza.

3- Recombinant Vaccines for poultry

4- Livestock Pharming – Products like

myelin production

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ANIMAL PRODUCTED FROM

TRANSGENIC

1- Transgenic Sheep

2- Transgenic Goat

3- Transgenic Pigs

4- Transgenic Cattle

5- Transgenic Fish

6- Transgenic Chicken

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REFERENCES

Robl JM, Wang Z, Kasinathan P, Kuroiwa Y (2007)

Transgenic animal production and animal

biotechnology. Theriogenology 67:127–133

Gorman, C. (1986). ‘High efficientcy gene transfer

into mammalian cells’, In: DNA cloning Vol. II,

apractical approach, ed., E.M. Glover., IRL Press,

Oxford

Jogdand, S.N. (2003). ‘Gene Biotechnology’,

Second reversed Edition, Himalaya Publishing

House.

Gordon, J.W. Ruddle, F.H.( 1983). ‘Gene trancfer

into mouse embryo’, first edition, Kalyani Publishing

House.

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TANK YOU

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